Click here to switch to the map view.

The map label for this gene is ispG

Identifier: 71892304

GI number: 71892304

Start: 659362

End: 660492

Strand: Reverse

Name: ispG

Synonym: BPEN_551

Alternate gene names: 71892304

Gene position: 660492-659362 (Counterclockwise)

Preceding gene: 71892305

Following gene: 71892303

Centisome position: 83.43

GC content: 34.57

Gene sequence:

>1131_bases
ATGAATAATGTTACGAACATTGTTCGTCGAAAATCTACACGTATTTATATTGGCAATGTTCCTATTGGAGATGGCGCTCC
AATTGCGGTACAATCCATGGTAAATACTCGTACTACAGATATTTTAGAAACAGTTACTCAAATTAACGCCCTAAAAAAAG
CAGGAGTAGATATTGTCCGAATATCGATACCTACTATGGATGCAGCTGAAGCTTTCAAAATAATTAAACAACAAGTAATT
AATACTCCATTAGTAGCAGATATTCATTTTGATTACCGTATTGCATTAAAAGTAGCTGAATACGGAGCAGATTGCCTACG
TATCAATCCCGGTAATATCGGGAATAATAAACGTATTCGATCTGTAGTGCATTGTGCTCAAGATAAAAATATATCTATTC
GTATTGGTGTAAATTCTGGATCTCTTGAGCGTGATTTACAAAAAAAGTATGACGGAAATCCAACAGAAAATGTATTATTA
GAATCAGCAATGAGACATGTAGATATTTTAGATAAATTAAACTTTCATCAATTTAAAGTTAGCGTTAAATCTTCTGATGT
GCTAATAACAATACAAGCCTACCGCGCATTGGCATCAAAAATAGATCAACCATTACATATAGGTCTTACTGAAGCTGGAG
CTTTGCGTAATGGGACAGTAAAATCTGCAATGGCTGTAGGTTTCTTATTAAACGAAGGCATCGGGGATACAATACGTATA
TCATTAGCTACAGATCCTATAGAAGAAGTTAAGGTGGCATTTGACATTTTAAGAGCTTTGAGAATTCGCGCTCATGGTAT
TAATTTTATAGCTTGTCCGGGATGTGCTCGACAAGAATTTAACGTAATCAATGTAGTTAATGCGTTAGAAGAAAAAGTAA
GTGATATTACAACGCCTATGGATGTATCTATTATTGGATGTGTAGTTAACGGACCAGGAGAAGCATTAAAATCAACCATT
GGTGTTGCCGGTGCACGTAATAAAAGTGGTATATATGAAGATGGTATACGGCAAAAAAAACGCTGCGATAACATATCAAT
AATTGAAGAATTAGAACGTCGTATTCGAGCTAAATCTAAATTATTAGACCCAAATAACCACAACATTACCCCTACCACAT
ATAATAAATAA

Upstream 100 bases:

>100_bases
CGATTCCTATTATAAATATAATTAAATCTCTTAATTGATATAATCAATAAATTCAAAAAATATATTTATTGTGTAAAAAC
ATAAAAATATGGGAGATTAT

Downstream 100 bases:

>100_bases
TTGAAGATTACAAATGCTTAAATATGATAAAAATATCCAATCTGTCCGCGGTATGCATGATTATTTGCCGAAAGATACGA
TTCTATGGCAATACATAGAA

Product: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNNVTNIVRRKSTRIYIGNVPIGDGAPIAVQSMVNTRTTDILETVTQINALKKAGVDIVRISIPTMDAAEAFKIIKQQVI
NTPLVADIHFDYRIALKVAEYGADCLRINPGNIGNNKRIRSVVHCAQDKNISIRIGVNSGSLERDLQKKYDGNPTENVLL
ESAMRHVDILDKLNFHQFKVSVKSSDVLITIQAYRALASKIDQPLHIGLTEAGALRNGTVKSAMAVGFLLNEGIGDTIRI
SLATDPIEEVKVAFDILRALRIRAHGINFIACPGCARQEFNVINVVNALEEKVSDITTPMDVSIIGCVVNGPGEALKSTI
GVAGARNKSGIYEDGIRQKKRCDNISIIEELERRIRAKSKLLDPNNHNITPTTYNK

Sequences:

>Translated_376_residues
MNNVTNIVRRKSTRIYIGNVPIGDGAPIAVQSMVNTRTTDILETVTQINALKKAGVDIVRISIPTMDAAEAFKIIKQQVI
NTPLVADIHFDYRIALKVAEYGADCLRINPGNIGNNKRIRSVVHCAQDKNISIRIGVNSGSLERDLQKKYDGNPTENVLL
ESAMRHVDILDKLNFHQFKVSVKSSDVLITIQAYRALASKIDQPLHIGLTEAGALRNGTVKSAMAVGFLLNEGIGDTIRI
SLATDPIEEVKVAFDILRALRIRAHGINFIACPGCARQEFNVINVVNALEEKVSDITTPMDVSIIGCVVNGPGEALKSTI
GVAGARNKSGIYEDGIRQKKRCDNISIIEELERRIRAKSKLLDPNNHNITPTTYNK
>Mature_376_residues
MNNVTNIVRRKSTRIYIGNVPIGDGAPIAVQSMVNTRTTDILETVTQINALKKAGVDIVRISIPTMDAAEAFKIIKQQVI
NTPLVADIHFDYRIALKVAEYGADCLRINPGNIGNNKRIRSVVHCAQDKNISIRIGVNSGSLERDLQKKYDGNPTENVLL
ESAMRHVDILDKLNFHQFKVSVKSSDVLITIQAYRALASKIDQPLHIGLTEAGALRNGTVKSAMAVGFLLNEGIGDTIRI
SLATDPIEEVKVAFDILRALRIRAHGINFIACPGCARQEFNVINVVNALEEKVSDITTPMDVSIIGCVVNGPGEALKSTI
GVAGARNKSGIYEDGIRQKKRCDNISIIEELERRIRAKSKLLDPNNHNITPTTYNK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=363, Percent_Identity=72.4517906336088, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_BLOPB (Q492E0)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278038.1
- STRING:   Q492E0
- GeneID:   3562941
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_551
- eggNOG:   COG0821
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- BioCyc:   CBLO291272:BPEN_551-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 41237; Mature: 41237

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVTNIVRRKSTRIYIGNVPIGDGAPIAVQSMVNTRTTDILETVTQINALKKAGVDIVR
CCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ISIPTMDAAEAFKIIKQQVINTPLVADIHFDYRIALKVAEYGADCLRINPGNIGNNKRIR
EECCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCEEEECCCCCCCCHHHH
SVVHCAQDKNISIRIGVNSGSLERDLQKKYDGNPTENVLLESAMRHVDILDKLNFHQFKV
HHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
SVKSSDVLITIQAYRALASKIDQPLHIGLTEAGALRNGTVKSAMAVGFLLNEGIGDTIRI
EEECCCEEEEHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SLATDPIEEVKVAFDILRALRIRAHGINFIACPGCARQEFNVINVVNALEEKVSDITTPM
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCC
DVSIIGCVVNGPGEALKSTIGVAGARNKSGIYEDGIRQKKRCDNISIIEELERRIRAKSK
CEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLDPNNHNITPTTYNK
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNVTNIVRRKSTRIYIGNVPIGDGAPIAVQSMVNTRTTDILETVTQINALKKAGVDIVR
CCCHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ISIPTMDAAEAFKIIKQQVINTPLVADIHFDYRIALKVAEYGADCLRINPGNIGNNKRIR
EECCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCEEEECCCCCCCCHHHH
SVVHCAQDKNISIRIGVNSGSLERDLQKKYDGNPTENVLLESAMRHVDILDKLNFHQFKV
HHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
SVKSSDVLITIQAYRALASKIDQPLHIGLTEAGALRNGTVKSAMAVGFLLNEGIGDTIRI
EEECCCEEEEHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SLATDPIEEVKVAFDILRALRIRAHGINFIACPGCARQEFNVINVVNALEEKVSDITTPM
EEECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCC
DVSIIGCVVNGPGEALKSTIGVAGARNKSGIYEDGIRQKKRCDNISIIEELERRIRAKSK
CEEEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLDPNNHNITPTTYNK
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA