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The map label for this gene is gltX

Identifier: 71892276

GI number: 71892276

Start: 623765

End: 625171

Strand: Reverse

Name: gltX

Synonym: BPEN_521

Alternate gene names: 71892276

Gene position: 625171-623765 (Counterclockwise)

Preceding gene: 71892277

Following gene: 71892274

Centisome position: 78.97

GC content: 30.77

Gene sequence:

>1407_bases
ATGAATATTAAAACTCGATTCGCACCCAGTCCTACAGGTTCCATACATATTGGAAATATTCGCACTGCTTTGTATTCTTG
GTTGTTTGCTCGAAAGCAAGGTGGTAAATTTTTATTACGTATAGAAGATTCTGATTTGCAACGTTCTATAGATGATACAG
TTGAGCTGATTGTTGCAGGGATGCGTTGGCTTAGTTTAGATTGGGATGAAGGTCCTTATTTTCAAACTAATAGGTTTAGT
CGTTACAATAGTATAATAAGTTATATGATTCAACATGATATGGCTTATAAGTGTTATTGTTCATCAGAAAGATTAGAATC
GTTGCGATCAAATCAAATAAAAAACGGAGAAAAACCTAAATATGATGGTTATTGTCGTTTTAAATCTACGGATATATATA
GTTCTTCTATTAGTTCTTATGTGGTACGTTTTTGTAATCCTCAGGATGGCGTAGTAATATTTCATGATCAAATCAGAGGT
ACAATTACATTTAATAACAAAGAATTAGATGATCTAATTATCCGCAGAGCCGATGGTTCTCCAACGTATAATTTTTGCGT
TGTAATAGACGATATGGATATGCAAATTACTCACATCATACGAGGTGAAGAACACATTAATAATACTCCGCGTCAGATTA
ATATTTTAAAAGCATTGCGAGCTCCGATACCAACGTATGCTCATGTCTCAATGATATTAGATAATAATTTAAAAAAATTA
TCAAAACGCTGTGGAACATTGGGTATTATGCAATATCGTAATGATGGATTTCTCCCTGAAGCAATACTTAATTATTTGGT
AAGGTTAGGATGGTCGCATGGAGATCAAGAAATTTTTAGTATTGAAGAGATGATAAAATATTTTGATTTGAGTAGAATTA
GTAAATCTCCTAGTATTTTAAATCTTGAAAAATTACTTTGGTTGAATCATTATTATATTAATCATTTACCAATAGACTAC
GTAGCTTCACATTTATCATGGCATATGCATCAGCAAAAAATCAATATACAAAATGGTCCAAAATTAACTGATATAATAAA
ATTATTTGCAAGACGTTCACGTACATTAAAAGAAATTGTAAATAATTGTCTATATTTTTATATAGATTTTGATTTATTCG
ATAATAAGGTAGCTAAAGATTATTTAAAACCTGTTGCGATAACACCATTAAAATTTTTAAGAAAAAAATTCAGTAATATA
GTTGATTGGACACCAGAGATTATTAAATCTATAATTATAGAAACCGTCAATGAATTTAATACTAGCATAGATAAAATAGG
TATGCCGCTTCGTGTAGCATTAACCGGAACAAATTGTTCACCAACGCTTAGTATTACCATACATGCAATTGGTCAGTCTC
GTGTATTAGAGCGTATTGATCAGGCTATACGCTATATTTCTACATAG

Upstream 100 bases:

>100_bases
GATTTTTTAATTGTATTTTATTAATGTGAAAACAAAATTTCATTTTGTATTGACTTAAATGAAAATTTAATGATTATCTA
CAGTCAGGTACTTAATTTTT

Downstream 100 bases:

>100_bases
TATTAGATGGGAATATAAAAACAATAATTGTTGTGCATATACTTACTTTTAATTATATCATGTTAAGGTCATATCGGCTA
TAATATGGAGTTTTAATAGT

Product: glutamate tRNA synthetase, catalytic subunit

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MNIKTRFAPSPTGSIHIGNIRTALYSWLFARKQGGKFLLRIEDSDLQRSIDDTVELIVAGMRWLSLDWDEGPYFQTNRFS
RYNSIISYMIQHDMAYKCYCSSERLESLRSNQIKNGEKPKYDGYCRFKSTDIYSSSISSYVVRFCNPQDGVVIFHDQIRG
TITFNNKELDDLIIRRADGSPTYNFCVVIDDMDMQITHIIRGEEHINNTPRQINILKALRAPIPTYAHVSMILDNNLKKL
SKRCGTLGIMQYRNDGFLPEAILNYLVRLGWSHGDQEIFSIEEMIKYFDLSRISKSPSILNLEKLLWLNHYYINHLPIDY
VASHLSWHMHQQKINIQNGPKLTDIIKLFARRSRTLKEIVNNCLYFYIDFDLFDNKVAKDYLKPVAITPLKFLRKKFSNI
VDWTPEIIKSIIIETVNEFNTSIDKIGMPLRVALTGTNCSPTLSITIHAIGQSRVLERIDQAIRYIST

Sequences:

>Translated_468_residues
MNIKTRFAPSPTGSIHIGNIRTALYSWLFARKQGGKFLLRIEDSDLQRSIDDTVELIVAGMRWLSLDWDEGPYFQTNRFS
RYNSIISYMIQHDMAYKCYCSSERLESLRSNQIKNGEKPKYDGYCRFKSTDIYSSSISSYVVRFCNPQDGVVIFHDQIRG
TITFNNKELDDLIIRRADGSPTYNFCVVIDDMDMQITHIIRGEEHINNTPRQINILKALRAPIPTYAHVSMILDNNLKKL
SKRCGTLGIMQYRNDGFLPEAILNYLVRLGWSHGDQEIFSIEEMIKYFDLSRISKSPSILNLEKLLWLNHYYINHLPIDY
VASHLSWHMHQQKINIQNGPKLTDIIKLFARRSRTLKEIVNNCLYFYIDFDLFDNKVAKDYLKPVAITPLKFLRKKFSNI
VDWTPEIIKSIIIETVNEFNTSIDKIGMPLRVALTGTNCSPTLSITIHAIGQSRVLERIDQAIRYIST
>Mature_468_residues
MNIKTRFAPSPTGSIHIGNIRTALYSWLFARKQGGKFLLRIEDSDLQRSIDDTVELIVAGMRWLSLDWDEGPYFQTNRFS
RYNSIISYMIQHDMAYKCYCSSERLESLRSNQIKNGEKPKYDGYCRFKSTDIYSSSISSYVVRFCNPQDGVVIFHDQIRG
TITFNNKELDDLIIRRADGSPTYNFCVVIDDMDMQITHIIRGEEHINNTPRQINILKALRAPIPTYAHVSMILDNNLKKL
SKRCGTLGIMQYRNDGFLPEAILNYLVRLGWSHGDQEIFSIEEMIKYFDLSRISKSPSILNLEKLLWLNHYYINHLPIDY
VASHLSWHMHQQKINIQNGPKLTDIIKLFARRSRTLKEIVNNCLYFYIDFDLFDNKVAKDYLKPVAITPLKFLRKKFSNI
VDWTPEIIKSIIIETVNEFNTSIDKIGMPLRVALTGTNCSPTLSITIHAIGQSRVLERIDQAIRYIST

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=330, Percent_Identity=32.7272727272727, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788743, Length=467, Percent_Identity=61.4561027837259, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=239, Percent_Identity=33.8912133891213, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=317, Percent_Identity=38.801261829653, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=29.3522267206478, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321192, Length=231, Percent_Identity=25.1082251082251, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=29.0983606557377, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_BLOPB (Q492G6)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278010.1
- ProteinModelPortal:   Q492G6
- SMR:   Q492G6
- STRING:   Q492G6
- GeneID:   3562945
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_521
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- BioCyc:   CBLO291272:BPEN_521-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 54323; Mature: 54323

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKTRFAPSPTGSIHIGNIRTALYSWLFARKQGGKFLLRIEDSDLQRSIDDTVELIVAG
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHH
MRWLSLDWDEGPYFQTNRFSRYNSIISYMIQHDMAYKCYCSSERLESLRSNQIKNGEKPK
HHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCC
YDGYCRFKSTDIYSSSISSYVVRFCNPQDGVVIFHDQIRGTITFNNKELDDLIIRRADGS
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCHHHHHEECCCCC
PTYNFCVVIDDMDMQITHIIRGEEHINNTPRQINILKALRAPIPTYAHVSMILDNNLKKL
CCEEEEEEEECCCCEEHHEECCHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHH
SKRCGTLGIMQYRNDGFLPEAILNYLVRLGWSHGDQEIFSIEEMIKYFDLSRISKSPSIL
HHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
NLEKLLWLNHYYINHLPIDYVASHLSWHMHQQKINIQNGPKLTDIIKLFARRSRTLKEIV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHH
NNCLYFYIDFDLFDNKVAKDYLKPVAITPLKFLRKKFSNIVDWTPEIIKSIIIETVNEFN
HCCEEEEEEEEHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TSIDKIGMPLRVALTGTNCSPTLSITIHAIGQSRVLERIDQAIRYIST
CHHHHCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIKTRFAPSPTGSIHIGNIRTALYSWLFARKQGGKFLLRIEDSDLQRSIDDTVELIVAG
CCCCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHH
MRWLSLDWDEGPYFQTNRFSRYNSIISYMIQHDMAYKCYCSSERLESLRSNQIKNGEKPK
HHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCC
YDGYCRFKSTDIYSSSISSYVVRFCNPQDGVVIFHDQIRGTITFNNKELDDLIIRRADGS
CCCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCHHHHHEECCCCC
PTYNFCVVIDDMDMQITHIIRGEEHINNTPRQINILKALRAPIPTYAHVSMILDNNLKKL
CCEEEEEEEECCCCEEHHEECCHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCHHHH
SKRCGTLGIMQYRNDGFLPEAILNYLVRLGWSHGDQEIFSIEEMIKYFDLSRISKSPSIL
HHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
NLEKLLWLNHYYINHLPIDYVASHLSWHMHQQKINIQNGPKLTDIIKLFARRSRTLKEIV
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHH
NNCLYFYIDFDLFDNKVAKDYLKPVAITPLKFLRKKFSNIVDWTPEIIKSIIIETVNEFN
HCCEEEEEEEEHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TSIDKIGMPLRVALTGTNCSPTLSITIHAIGQSRVLERIDQAIRYIST
CHHHHCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA