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The map label for this gene is ligA

Identifier: 71892277

GI number: 71892277

Start: 625784

End: 627571

Strand: Reverse

Name: ligA

Synonym: BPEN_524

Alternate gene names: 71892277

Gene position: 627571-625784 (Counterclockwise)

Preceding gene: 71892281

Following gene: 71892276

Centisome position: 79.27

GC content: 30.82

Gene sequence:

>1788_bases
ATGAAATTTGTTAAACAAAAAATACAAAAATTAAGAAAAAAATTACGTCATTGGGAATATTTATACTATACAAAAAATGA
ATCTGCAGTATCCGATGAAAAGTATGATGCGATGTTAGAAAAATTAAATCAGTTGGAACAAATTTATCCACATTTGATTG
CAGAAAGTTCTCCTACTCAACGTATAGGAGGTGTGTCTCAATATAATTTCAAAAAAATACATCATAAGGTGCCAATGCTA
TCTTTAAATAGTATCGTTGCATCGTTTCAATTATTATCATTTGATAAAAGAATAAAAATTAAACTACATGCTAATCATGT
TATGTCATATTGTTGTGAATTAAAAATAGATGGGGTGGCAGTGAGTTTATTGTATAAAGAAGGGAAATTAATTTATGCGG
CAACTCGAGGTGATGGTAAAATCGGAGAGGATGTTACTGAAAATATAAGCACTATTCGCGCAGTACCTATGTGTTTAAAA
ATAGATTCTAATAAATATGGTAAATTACCATATTTATTAGAAATTAGAGGAGAAGTATTTATATCTAAATTATGTTTTTT
AGAATTGAACAAAATAACGATACAACAAGGCAACAAACCTTTTTCTAATGCGCGAAATGCTGCTTCTGGTTCCTTGCGTC
AGTTAGATCCTAGTGTTACAGCCACACGCCCATTAAGTTTTTATTGTTATGGCATTAGTAATTATTGCGGAGAAAAGGAA
TTACCAGATAGTCATTGGGAACGATTACAATTATGCGAAAATTGGGGTTTACCGATTAATAATTATATCCGCTTAATTAG
TGGAGTTAATAAAGTATTAGAATATTATTCTTATATAAAGACAATACGATCTAATTTGGAATTTAACATTGATGGAATAG
TTATTAAGGTAAATAGTTGCGCATATCAGAGTAAATTAGGTTGTGGATCTAGAGCACCTCATTGGGCTCTTGCCTATAAA
TTTCCTTCGGAAGTAAGTTCTGCTAAAGTAGATAACGTAATTTTCCAAGTTGGGCGAACAGGTATTATAACTCCAATAGC
TTATTTAGAGCCTATTGTAATTTCTGATGTTACAATTAGAAAAGTAAACATGCATAACATCAATGAAGTTAAAAGACTTG
GATTAATGATTGGCGATACAGTTCGTATACAAAGATCTGGCGATGTGATTCCTAAAATTGTAGAAGTAATTTTATCAGAA
CGTACCGATCATGTAAAAACTATAGAATTACCCCGATTTTGTCCGGTATGCGGGTCTCGTATTAAAACATGGCGTAATCA
ATCAATATTACGCTGTACGGCCGGATTATCGTGCCTTGCTCAACGAAAGGCAACGCTTGAGCATTTTGTTTCTAAAAAAG
CAATGAACATTTATGGAATGGGAAATAAAATTATTGATCAATTAGTTAATCAAGGTTTAATATTTACTTCATCTGATGTT
TTTCGTTTAAACAAAAATAAGTTACTTTGTTTAGAGGGCTTTGGTTTAGAAAATATTGAACGTTTATTAAGGTCTATCGA
GGATTCTAAAAAAATTACTTTGGCTCGTTTTATATATGCGCTAGGTATTTATGGTGTAGGTGAAACAGTAGCTAGTAATT
TAGCTATTGTATATAAAACTATAGAAAATTTGGCCGCTGCTGATTTGCAATCATTATCAAATTTGAAATATGTAGGGCCA
ATTATAGCTAATAATATATATCATTTTTTCAGAAATCCAGACAATTTAAAAAATGTTCAAGATTTAATTGATCCGGCTAT
TGGTATTCAGTTATATGTTATTACATGA

Upstream 100 bases:

>100_bases
AGATATTTTTATTATAAAATGTTAAATCACGTGTAAATAAAAATAACGATTTTAAATGTGAATTAATTATCGCAACCCAT
ATAATTGGGATATTTATATT

Downstream 100 bases:

>100_bases
AAATAAGAATTTAAAAGATTAAAAAATTATAGGTATCTCAAAATTAGAGTTGTGTATATTAGCGATATCTATGATTAACA
ATTAAAAGGATGCAAATTTA

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MKFVKQKIQKLRKKLRHWEYLYYTKNESAVSDEKYDAMLEKLNQLEQIYPHLIAESSPTQRIGGVSQYNFKKIHHKVPML
SLNSIVASFQLLSFDKRIKIKLHANHVMSYCCELKIDGVAVSLLYKEGKLIYAATRGDGKIGEDVTENISTIRAVPMCLK
IDSNKYGKLPYLLEIRGEVFISKLCFLELNKITIQQGNKPFSNARNAASGSLRQLDPSVTATRPLSFYCYGISNYCGEKE
LPDSHWERLQLCENWGLPINNYIRLISGVNKVLEYYSYIKTIRSNLEFNIDGIVIKVNSCAYQSKLGCGSRAPHWALAYK
FPSEVSSAKVDNVIFQVGRTGIITPIAYLEPIVISDVTIRKVNMHNINEVKRLGLMIGDTVRIQRSGDVIPKIVEVILSE
RTDHVKTIELPRFCPVCGSRIKTWRNQSILRCTAGLSCLAQRKATLEHFVSKKAMNIYGMGNKIIDQLVNQGLIFTSSDV
FRLNKNKLLCLEGFGLENIERLLRSIEDSKKITLARFIYALGIYGVGETVASNLAIVYKTIENLAAADLQSLSNLKYVGP
IIANNIYHFFRNPDNLKNVQDLIDPAIGIQLYVIT

Sequences:

>Translated_595_residues
MKFVKQKIQKLRKKLRHWEYLYYTKNESAVSDEKYDAMLEKLNQLEQIYPHLIAESSPTQRIGGVSQYNFKKIHHKVPML
SLNSIVASFQLLSFDKRIKIKLHANHVMSYCCELKIDGVAVSLLYKEGKLIYAATRGDGKIGEDVTENISTIRAVPMCLK
IDSNKYGKLPYLLEIRGEVFISKLCFLELNKITIQQGNKPFSNARNAASGSLRQLDPSVTATRPLSFYCYGISNYCGEKE
LPDSHWERLQLCENWGLPINNYIRLISGVNKVLEYYSYIKTIRSNLEFNIDGIVIKVNSCAYQSKLGCGSRAPHWALAYK
FPSEVSSAKVDNVIFQVGRTGIITPIAYLEPIVISDVTIRKVNMHNINEVKRLGLMIGDTVRIQRSGDVIPKIVEVILSE
RTDHVKTIELPRFCPVCGSRIKTWRNQSILRCTAGLSCLAQRKATLEHFVSKKAMNIYGMGNKIIDQLVNQGLIFTSSDV
FRLNKNKLLCLEGFGLENIERLLRSIEDSKKITLARFIYALGIYGVGETVASNLAIVYKTIENLAAADLQSLSNLKYVGP
IIANNIYHFFRNPDNLKNVQDLIDPAIGIQLYVIT
>Mature_595_residues
MKFVKQKIQKLRKKLRHWEYLYYTKNESAVSDEKYDAMLEKLNQLEQIYPHLIAESSPTQRIGGVSQYNFKKIHHKVPML
SLNSIVASFQLLSFDKRIKIKLHANHVMSYCCELKIDGVAVSLLYKEGKLIYAATRGDGKIGEDVTENISTIRAVPMCLK
IDSNKYGKLPYLLEIRGEVFISKLCFLELNKITIQQGNKPFSNARNAASGSLRQLDPSVTATRPLSFYCYGISNYCGEKE
LPDSHWERLQLCENWGLPINNYIRLISGVNKVLEYYSYIKTIRSNLEFNIDGIVIKVNSCAYQSKLGCGSRAPHWALAYK
FPSEVSSAKVDNVIFQVGRTGIITPIAYLEPIVISDVTIRKVNMHNINEVKRLGLMIGDTVRIQRSGDVIPKIVEVILSE
RTDHVKTIELPRFCPVCGSRIKTWRNQSILRCTAGLSCLAQRKATLEHFVSKKAMNIYGMGNKIIDQLVNQGLIFTSSDV
FRLNKNKLLCLEGFGLENIERLLRSIEDSKKITLARFIYALGIYGVGETVASNLAIVYKTIENLAAADLQSLSNLKYVGP
IIANNIYHFFRNPDNLKNVQDLIDPAIGIQLYVIT

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigA subfamily

Homologues:

Organism=Escherichia coli, GI1788750, Length=583, Percent_Identity=45.1114922813036, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI87082305, Length=402, Percent_Identity=24.6268656716418, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BLOPB (Q492G5)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_278011.1
- ProteinModelPortal:   Q492G5
- SMR:   Q492G5
- STRING:   Q492G5
- GeneID:   3562951
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_524
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   HNQDYID
- ProtClustDB:   CLSK280905
- BioCyc:   CBLO291272:BPEN_524-MONOMER
- HAMAP:   MF_01588
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 67382; Mature: 67382

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 178-178 BINDING 296-296 BINDING 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVKQKIQKLRKKLRHWEYLYYTKNESAVSDEKYDAMLEKLNQLEQIYPHLIAESSPTQ
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RIGGVSQYNFKKIHHKVPMLSLNSIVASFQLLSFDKRIKIKLHANHVMSYCCELKIDGVA
HHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCEEEEEEEHHHHHHHHHHEEECCEE
VSLLYKEGKLIYAATRGDGKIGEDVTENISTIRAVPMCLKIDSNKYGKLPYLLEIRGEVF
EEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEHHHHH
ISKLCFLELNKITIQQGNKPFSNARNAASGSLRQLDPSVTATRPLSFYCYGISNYCGEKE
HHHHHHHEECEEEEECCCCCCHHCCCCCCCCHHHCCCCCCCCCCCEEEEEEHHHHCCCCC
LPDSHWERLQLCENWGLPINNYIRLISGVNKVLEYYSYIKTIRSNLEFNIDGIVIKVNSC
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECC
AYQSKLGCGSRAPHWALAYKFPSEVSSAKVDNVIFQVGRTGIITPIAYLEPIVISDVTIR
CHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEECCEEEE
KVNMHNINEVKRLGLMIGDTVRIQRSGDVIPKIVEVILSERTDHVKTIELPRFCPVCGSR
EECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
IKTWRNQSILRCTAGLSCLAQRKATLEHFVSKKAMNIYGMGNKIIDQLVNQGLIFTSSDV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCEEEECCCE
FRLNKNKLLCLEGFGLENIERLLRSIEDSKKITLARFIYALGIYGVGETVASNLAIVYKT
EEECCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IENLAAADLQSLSNLKYVGPIIANNIYHFFRNPDNLKNVQDLIDPAIGIQLYVIT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MKFVKQKIQKLRKKLRHWEYLYYTKNESAVSDEKYDAMLEKLNQLEQIYPHLIAESSPTQ
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
RIGGVSQYNFKKIHHKVPMLSLNSIVASFQLLSFDKRIKIKLHANHVMSYCCELKIDGVA
HHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCEEEEEEEHHHHHHHHHHEEECCEE
VSLLYKEGKLIYAATRGDGKIGEDVTENISTIRAVPMCLKIDSNKYGKLPYLLEIRGEVF
EEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEHHHHH
ISKLCFLELNKITIQQGNKPFSNARNAASGSLRQLDPSVTATRPLSFYCYGISNYCGEKE
HHHHHHHEECEEEEECCCCCCHHCCCCCCCCHHHCCCCCCCCCCCEEEEEEHHHHCCCCC
LPDSHWERLQLCENWGLPINNYIRLISGVNKVLEYYSYIKTIRSNLEFNIDGIVIKVNSC
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECC
AYQSKLGCGSRAPHWALAYKFPSEVSSAKVDNVIFQVGRTGIITPIAYLEPIVISDVTIR
CHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEECCEEEE
KVNMHNINEVKRLGLMIGDTVRIQRSGDVIPKIVEVILSERTDHVKTIELPRFCPVCGSR
EECCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHH
IKTWRNQSILRCTAGLSCLAQRKATLEHFVSKKAMNIYGMGNKIIDQLVNQGLIFTSSDV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCEEEECCCE
FRLNKNKLLCLEGFGLENIERLLRSIEDSKKITLARFIYALGIYGVGETVASNLAIVYKT
EEECCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IENLAAADLQSLSNLKYVGPIIANNIYHFFRNPDNLKNVQDLIDPAIGIQLYVIT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA