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The map label for this gene is trpE [H]

Identifier: 71892200

GI number: 71892200

Start: 518841

End: 520403

Strand: Direct

Name: trpE [H]

Synonym: BPEN_438

Alternate gene names: 71892200

Gene position: 518841-520403 (Clockwise)

Preceding gene: 71892199

Following gene: 71892201

Centisome position: 65.54

GC content: 34.74

Gene sequence:

>1563_bases
ATGAAATATCAAAAAAATCAGATGAAATGTCTAAATATTCAAGTGCGCTATCATTCTAATCCAGCAGCCGTTTTTAATCA
ATTATGTAATAAACGTAATTCGACATTATTATTAGAATCAGCAGAAATTAATAAAAAACACAACATAGAAAGTATGATGA
TTGTTGATAGCGCATTGCGTATTACCGCTTATGGATCAACTGTTACTGTACAAGCTTTAACTAAAAATGGTGCAAGTTTA
CTTCCATTACTAGTACAAACATTACCCACAAAAATACAAATTCATAAAACTTCTAACAGCATCAAATTTATGTTTCCTAC
AACACAGACCATGACTGACGAAGATACACGTCTACGATCGATTTCAATATTTGATTGTCTACGCTCATTGTTAAAAATAA
TACAACCTCTTCATGATACCCCCAAAGCTACATTTTTAGGAGGTTTATTTTCCTACGATTTAATATCTTGTTTTGAATAT
TTTCCTCGGTTAATCAATACTTATACATGCCCAGACTACTGTTTCTATTTAGCCGAAACATTATTAGTATTTGATCATCA
ACAATATACTGCTTCGTTACAAATTACCTTGTTTAGTCCAAATATCTTAGAAAAAGATCGATTACAAACTAGGATGAATC
AATTAAAAACCCAAATTAACCAAACACCGCAATCTATTCCTTATAAAAAAATACATAATATGACATTACGCTGTGATCCA
AATGACGAAAAATACAAACGAGTTATACGTAAAATACAAAGATCTATACGTCAAGGAGAAATATTACAAGCTGTCCCTTC
ACGACGATTTTTCCTTCCCTGTCCTTCACCATTAGCAGCATACCATGTATTAAAAACTAATAATCCTAGTCCCTACATGT
TTTTTATGCAAGACATAGCATTTACACTTTTCGGAGCTTCTCCTGAAAGTTCTTTAAAATACAATGCAGATACTCGACAA
ATTGAAATTTACCCCATCGCTGGTACACGAGCTAGAACTTATCGTACTGACGGATCATTGGATACAGATGCAGATAACCG
TATAGAATTAGAAATGCGCTTAAATCATAAAGAATTATCAGAACATCTCATGTTAGTAGACTTAGCAAGAAATGATTTAG
CACGTGTCTGTAAAACGGGTAGCCGTTATGTCGCTGATTTATTAAAAGTAGATAGATACTCTTTTGTTATGCATTTAGTG
TCTAGAGTGGTGGGAGAATTGCGTCATGATCTTGATATACTACATGCCTATCGTGCATGTATGAATATGGGCACATTAAC
TGGAGCTCCAAAAATTAGAGCCATGGAACTGATTTATGAAGTAGAAGGTACACGTCGTAACACTTACGGAGGCGCTATTG
GATACCTAACTGGTGCTGGAAATTTAGACACATGTATCATCATTCGATCAGCCTACGTCACAAATCAAATAGCTACAATA
CAAGCAGGAGCCGGTGTGGTAATAGATTCCATACCTCAATCAGAAGCTGATGAAAGCCGTAGCAAAGCGCGCGCTGTTTT
ACATGCCATTTCTACAGCACATGGTTCTAAGGAGTTATTTTAA

Upstream 100 bases:

>100_bases
CAATTATTTAGAATATTACTATGATTGAAAATATTGCAATTAGATCCTTAAATATAAATGAGGTTTTTGATAAATAAATC
ACTGGGAAATTTAAAAAATA

Downstream 100 bases:

>100_bases
CAATGGTTAATGTAATATTACTAGATAACATTGACTCATTTACGTATAATTTAGTAGATCAGTTACGCAATCACGGCCAT
CAAGTACTAATATATACTAA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKYQKNQMKCLNIQVRYHSNPAAVFNQLCNKRNSTLLLESAEINKKHNIESMMIVDSALRITAYGSTVTVQALTKNGASL
LPLLVQTLPTKIQIHKTSNSIKFMFPTTQTMTDEDTRLRSISIFDCLRSLLKIIQPLHDTPKATFLGGLFSYDLISCFEY
FPRLINTYTCPDYCFYLAETLLVFDHQQYTASLQITLFSPNILEKDRLQTRMNQLKTQINQTPQSIPYKKIHNMTLRCDP
NDEKYKRVIRKIQRSIRQGEILQAVPSRRFFLPCPSPLAAYHVLKTNNPSPYMFFMQDIAFTLFGASPESSLKYNADTRQ
IEIYPIAGTRARTYRTDGSLDTDADNRIELEMRLNHKELSEHLMLVDLARNDLARVCKTGSRYVADLLKVDRYSFVMHLV
SRVVGELRHDLDILHAYRACMNMGTLTGAPKIRAMELIYEVEGTRRNTYGGAIGYLTGAGNLDTCIIIRSAYVTNQIATI
QAGAGVVIDSIPQSEADESRSKARAVLHAISTAHGSKELF

Sequences:

>Translated_520_residues
MKYQKNQMKCLNIQVRYHSNPAAVFNQLCNKRNSTLLLESAEINKKHNIESMMIVDSALRITAYGSTVTVQALTKNGASL
LPLLVQTLPTKIQIHKTSNSIKFMFPTTQTMTDEDTRLRSISIFDCLRSLLKIIQPLHDTPKATFLGGLFSYDLISCFEY
FPRLINTYTCPDYCFYLAETLLVFDHQQYTASLQITLFSPNILEKDRLQTRMNQLKTQINQTPQSIPYKKIHNMTLRCDP
NDEKYKRVIRKIQRSIRQGEILQAVPSRRFFLPCPSPLAAYHVLKTNNPSPYMFFMQDIAFTLFGASPESSLKYNADTRQ
IEIYPIAGTRARTYRTDGSLDTDADNRIELEMRLNHKELSEHLMLVDLARNDLARVCKTGSRYVADLLKVDRYSFVMHLV
SRVVGELRHDLDILHAYRACMNMGTLTGAPKIRAMELIYEVEGTRRNTYGGAIGYLTGAGNLDTCIIIRSAYVTNQIATI
QAGAGVVIDSIPQSEADESRSKARAVLHAISTAHGSKELF
>Mature_520_residues
MKYQKNQMKCLNIQVRYHSNPAAVFNQLCNKRNSTLLLESAEINKKHNIESMMIVDSALRITAYGSTVTVQALTKNGASL
LPLLVQTLPTKIQIHKTSNSIKFMFPTTQTMTDEDTRLRSISIFDCLRSLLKIIQPLHDTPKATFLGGLFSYDLISCFEY
FPRLINTYTCPDYCFYLAETLLVFDHQQYTASLQITLFSPNILEKDRLQTRMNQLKTQINQTPQSIPYKKIHNMTLRCDP
NDEKYKRVIRKIQRSIRQGEILQAVPSRRFFLPCPSPLAAYHVLKTNNPSPYMFFMQDIAFTLFGASPESSLKYNADTRQ
IEIYPIAGTRARTYRTDGSLDTDADNRIELEMRLNHKELSEHLMLVDLARNDLARVCKTGSRYVADLLKVDRYSFVMHLV
SRVVGELRHDLDILHAYRACMNMGTLTGAPKIRAMELIYEVEGTRRNTYGGAIGYLTGAGNLDTCIIIRSAYVTNQIATI
QAGAGVVIDSIPQSEADESRSKARAVLHAISTAHGSKELF

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=520, Percent_Identity=61.7307692307692, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI1788114, Length=402, Percent_Identity=31.3432835820896, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI87082077, Length=332, Percent_Identity=25.9036144578313, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=428, Percent_Identity=32.4766355140187, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324361, Length=355, Percent_Identity=25.9154929577465, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 59040; Mature: 59040

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQKNQMKCLNIQVRYHSNPAAVFNQLCNKRNSTLLLESAEINKKHNIESMMIVDSALR
CCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEHHCEE
ITAYGSTVTVQALTKNGASLLPLLVQTLPTKIQIHKTSNSIKFMFPTTQTMTDEDTRLRS
EEEECCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHE
ISIFDCLRSLLKIIQPLHDTPKATFLGGLFSYDLISCFEYFPRLINTYTCPDYCFYLAET
EHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LLVFDHQQYTASLQITLFSPNILEKDRLQTRMNQLKTQINQTPQSIPYKKIHNMTLRCDP
HHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEECC
NDEKYKRVIRKIQRSIRQGEILQAVPSRRFFLPCPSPLAAYHVLKTNNPSPYMFFMQDIA
CHHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCHHHHEEEEECCCCCCEEHHHHHHH
FTLFGASPESSLKYNADTRQIEIYPIAGTRARTYRTDGSLDTDADNRIELEMRLNHKELS
HHHCCCCCCCCEEECCCCEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEEECCHHHHH
EHLMLVDLARNDLARVCKTGSRYVADLLKVDRYSFVMHLVSRVVGELRHDLDILHAYRAC
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLTGAPKIRAMELIYEVEGTRRNTYGGAIGYLTGAGNLDTCIIIRSAYVTNQIATI
HHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEEEHHHHCCEEEE
QAGAGVVIDSIPQSEADESRSKARAVLHAISTAHGSKELF
ECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKYQKNQMKCLNIQVRYHSNPAAVFNQLCNKRNSTLLLESAEINKKHNIESMMIVDSALR
CCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEHHCEE
ITAYGSTVTVQALTKNGASLLPLLVQTLPTKIQIHKTSNSIKFMFPTTQTMTDEDTRLRS
EEEECCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHE
ISIFDCLRSLLKIIQPLHDTPKATFLGGLFSYDLISCFEYFPRLINTYTCPDYCFYLAET
EHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LLVFDHQQYTASLQITLFSPNILEKDRLQTRMNQLKTQINQTPQSIPYKKIHNMTLRCDP
HHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEECC
NDEKYKRVIRKIQRSIRQGEILQAVPSRRFFLPCPSPLAAYHVLKTNNPSPYMFFMQDIA
CHHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCCHHHHEEEEECCCCCCEEHHHHHHH
FTLFGASPESSLKYNADTRQIEIYPIAGTRARTYRTDGSLDTDADNRIELEMRLNHKELS
HHHCCCCCCCCEEECCCCEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEEECCHHHHH
EHLMLVDLARNDLARVCKTGSRYVADLLKVDRYSFVMHLVSRVVGELRHDLDILHAYRAC
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLTGAPKIRAMELIYEVEGTRRNTYGGAIGYLTGAGNLDTCIIIRSAYVTNQIATI
HHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEEEHHHHCCEEEE
QAGAGVVIDSIPQSEADESRSKARAVLHAISTAHGSKELF
ECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7038627; 7021857; 9097039; 9278503; 351195; 4598537 [H]