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The map label for this gene is holB [H]

Identifier: 71892179

GI number: 71892179

Start: 488141

End: 489142

Strand: Reverse

Name: holB [H]

Synonym: BPEN_412

Alternate gene names: 71892179

Gene position: 489142-488141 (Counterclockwise)

Preceding gene: 71892180

Following gene: 71892178

Centisome position: 61.79

GC content: 29.44

Gene sequence:

>1002_bases
ATGAGATGGTATCCTTGGCTAGATAGTACTTATAATCAAATATTAAATGCTTATCGACAAGATAGAGGACATCATGCTTT
ACTTTTAAATAGTAAACGAAACAATGGTGAAAATACTTTAATTTATGCTATTGCACGTTGGTTAATTTGTTCTCATCCTG
ACGCAAATCAATGTTGCAATATTTGTTATGATTGTAGATTAATGAACGTAGGACATCATCCAGATTATTACGAAATAAGT
CCTGAAAATAATACTCAAACTATAGGTGTTGATACTATCCGTGCTTGTATTAATTCTCTACACAAATATGCATACCAAAG
TAAAGTAAAAATTATATATATAGAATATGTGGAATGTTTAACGGATCAGGCTGTTAATGTATTGTTAAAAACACTTGATG
AACCACCAACAAATACTTATTTTTTTTTTAAAACTCGAGATTATATGAGAATATCATTAACGTTGTTGAGTCGGTGTATG
CGATGGCCTATTATATCTCCTAAAGAATCGATTGGATTACAGTGGTTAATGAAAAATAGAGAAGAGATAAATGATATTTT
GTTGGCGCAGTGTGCATTACGTTTGTGTGATGGATCTCCTATAGAAGCTGAAGATATGTTTAAATTAGGTTTATGGAAAC
AACGATTAGAATTGTGCAAATGTATAAATTATGTCATTGCAAACGGGGATTTTTTAAAACTTGTGCCATTTTTAAATATT
TGTAAGAATGATATATATTTGTATTGGTTTATAACTGTACTTGTAGATGCATTAAAATGGAAGCAAGGGATAAAAAAAAG
ATTTTTAATAAATTTAGATCAAGTAGACTTGATTACTGTTATTACAGTTCATTGGGGTATTGTATCTTTAAGTCGTCAGT
TACAACAATGGTTGGTGCTGTTACGTTATTTTCAAGAATTTACTAATATTGATCGCGAATTATTATTGACTTATCGATTA
TTAAATTGGCAACAAGATATTGTTGAATCTTGTTTGCATTAA

Upstream 100 bases:

>100_bases
TATTATCACGATTGATGCTACTCAGAGTTTAGAAGAAGTTTCCATGTCTATTTATAGACATTTAGATTGGTGGTTCTTGC
GTCCGCAGGAATAATCTATA

Downstream 100 bases:

>100_bases
TGGAGTGTATAATGTTATGTTTTTGGTGGATTCCCATTGTCATTTAAATCAACTGAATTATCAAGATGTTCACAAAAATG
TTTCAGATGTGTTGAACAAA

Product: DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRWYPWLDSTYNQILNAYRQDRGHHALLLNSKRNNGENTLIYAIARWLICSHPDANQCCNICYDCRLMNVGHHPDYYEIS
PENNTQTIGVDTIRACINSLHKYAYQSKVKIIYIEYVECLTDQAVNVLLKTLDEPPTNTYFFFKTRDYMRISLTLLSRCM
RWPIISPKESIGLQWLMKNREEINDILLAQCALRLCDGSPIEAEDMFKLGLWKQRLELCKCINYVIANGDFLKLVPFLNI
CKNDIYLYWFITVLVDALKWKQGIKKRFLINLDQVDLITVITVHWGIVSLSRQLQQWLVLLRYFQEFTNIDRELLLTYRL
LNWQQDIVESCLH

Sequences:

>Translated_333_residues
MRWYPWLDSTYNQILNAYRQDRGHHALLLNSKRNNGENTLIYAIARWLICSHPDANQCCNICYDCRLMNVGHHPDYYEIS
PENNTQTIGVDTIRACINSLHKYAYQSKVKIIYIEYVECLTDQAVNVLLKTLDEPPTNTYFFFKTRDYMRISLTLLSRCM
RWPIISPKESIGLQWLMKNREEINDILLAQCALRLCDGSPIEAEDMFKLGLWKQRLELCKCINYVIANGDFLKLVPFLNI
CKNDIYLYWFITVLVDALKWKQGIKKRFLINLDQVDLITVITVHWGIVSLSRQLQQWLVLLRYFQEFTNIDRELLLTYRL
LNWQQDIVESCLH
>Mature_333_residues
MRWYPWLDSTYNQILNAYRQDRGHHALLLNSKRNNGENTLIYAIARWLICSHPDANQCCNICYDCRLMNVGHHPDYYEIS
PENNTQTIGVDTIRACINSLHKYAYQSKVKIIYIEYVECLTDQAVNVLLKTLDEPPTNTYFFFKTRDYMRISLTLLSRCM
RWPIISPKESIGLQWLMKNREEINDILLAQCALRLCDGSPIEAEDMFKLGLWKQRLELCKCINYVIANGDFLKLVPFLNI
CKNDIYLYWFITVLVDALKWKQGIKKRFLINLDQVDLITVITVHWGIVSLSRQLQQWLVLLRYFQEFTNIDRELLLTYRL
LNWQQDIVESCLH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=321, Percent_Identity=36.7601246105919, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1786676, Length=149, Percent_Identity=28.8590604026846, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39478; Mature: 39478

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWYPWLDSTYNQILNAYRQDRGHHALLLNSKRNNGENTLIYAIARWLICSHPDANQCCN
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
ICYDCRLMNVGHHPDYYEISPENNTQTIGVDTIRACINSLHKYAYQSKVKIIYIEYVECL
HHHHCEEEECCCCCCCEEECCCCCCEEECHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHH
TDQAVNVLLKTLDEPPTNTYFFFKTRDYMRISLTLLSRCMRWPIISPKESIGLQWLMKNR
HHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCH
EEINDILLAQCALRLCDGSPIEAEDMFKLGLWKQRLELCKCINYVIANGDFLKLVPFLNI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
CKNDIYLYWFITVLVDALKWKQGIKKRFLINLDQVDLITVITVHWGIVSLSRQLQQWLVL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRYFQEFTNIDRELLLTYRLLNWQQDIVESCLH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRWYPWLDSTYNQILNAYRQDRGHHALLLNSKRNNGENTLIYAIARWLICSHPDANQCCN
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
ICYDCRLMNVGHHPDYYEISPENNTQTIGVDTIRACINSLHKYAYQSKVKIIYIEYVECL
HHHHCEEEECCCCCCCEEECCCCCCEEECHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHH
TDQAVNVLLKTLDEPPTNTYFFFKTRDYMRISLTLLSRCMRWPIISPKESIGLQWLMKNR
HHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCH
EEINDILLAQCALRLCDGSPIEAEDMFKLGLWKQRLELCKCINYVIANGDFLKLVPFLNI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
CKNDIYLYWFITVLVDALKWKQGIKKRFLINLDQVDLITVITVHWGIVSLSRQLQQWLVL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRYFQEFTNIDRELLLTYRLLNWQQDIVESCLH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]