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The map label for this gene is purR [H]

Identifier: 66045394

GI number: 66045394

Start: 2510220

End: 2511236

Strand: Direct

Name: purR [H]

Synonym: Psyr_2154

Alternate gene names: 66045394

Gene position: 2510220-2511236 (Clockwise)

Preceding gene: 66045393

Following gene: 66045395

Centisome position: 41.19

GC content: 60.37

Gene sequence:

>1017_bases
ATGGCAACCATCAAGGATGTCGCGGCCCTGGCGGGGATCTCGTACACCACGGTTTCCCACGTGTTGAACAATACCCGGCC
GGTGAGCGAGCCGGTCAGGCTCAAGGTCGAAGCCGCCATTGCACAGCTCGATTATGTGCCCAGTGCAGTGGCGCGTTCGC
TGAAGGCCAAGACCACCTCGACCATCGGCCTGTTGATTCCCAACGGCATGAACCCGTATTTCGCCGAGTTGGCGCGGGGC
ATCGAGGATTACTGCGAGCGCAACGGTTTTTGCGTGATCCTCTGCAACTCCGATGATAATCCGGAGAAACAGCGTGGTTA
TCTGCGCGTGCTGCTGGAAAAGCGCGTGGACGGGCTGATTGTTTCTTCGGTGGGTGGCGACGCGGGTATTGCAGGTGGCC
TTGCCGAGGTACGTACGCCGCTGGTGATCGTTGACCGGGAGCTGGACGGTATCGATGCCGACATGGTCCGTATCGACCAT
GAACAGGGTGCTTATCTGGCAACCCGGCACTTGCTGGACCTGGGACATCGCGCCATTGCCTGTATTGGTGGGCCATTTCA
CAGCACAGTGGCCGAGATGCGTCTGGCGGGTTATCGGCGCGCCTTGCAACAGGCGTCCGTTCCGGTCCGTGCCGAATGGG
CGCTGCACAGTGAGTTCACCAGCTCTGCCGGTCATGAGGCAGCGTGTCAGCTTCTGGCAGCGCATCCGCCGACCGCGATT
TTTGCCGGCAACGACGTCATCGCCATCGGTGTGCTGCGCGCCGCCGCCGAGCGCTTGATTCGTGTGCCTCAGGACCTGTC
GGTGATCGGTTTCGATGATATCCAGATGAGTCGCTACGTTTATCCGGCGCTGACCACCGTGGGGCAGTCAATCATGCAAC
TGGGCGAAACCGCTGCAGAAATGCTGCTGAGCAGAATCGCCACACCCCATGCGCTTCCTGCCGAAAAAAGGCTCGTGACG
CCCAGCGTCGTGGTGCGCGAGTCTACGGCAGCGCCAAACACTTTATCCAACGAATGA

Upstream 100 bases:

>100_bases
GCGACCGAGCCGACCAAGCGGATCATCACCGGTGCGGTGATCGTGATTGCTGTGGTGCTGGACACCTACCGCAGCCACCG
GGCTCGGCGCCAGGGCTGAT

Downstream 100 bases:

>100_bases
CGCGCAAGCACAGCAACGGATTAATCAGCCATGCAAGCAAAAATAGTGATAGTAGGCAGTCTGAACATGGATCTGGTCAT
CCGCGCCCAGCGTCTGCCAC

Product: LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MATIKDVAALAGISYTTVSHVLNNTRPVSEPVRLKVEAAIAQLDYVPSAVARSLKAKTTSTIGLLIPNGMNPYFAELARG
IEDYCERNGFCVILCNSDDNPEKQRGYLRVLLEKRVDGLIVSSVGGDAGIAGGLAEVRTPLVIVDRELDGIDADMVRIDH
EQGAYLATRHLLDLGHRAIACIGGPFHSTVAEMRLAGYRRALQQASVPVRAEWALHSEFTSSAGHEAACQLLAAHPPTAI
FAGNDVIAIGVLRAAAERLIRVPQDLSVIGFDDIQMSRYVYPALTTVGQSIMQLGETAAEMLLSRIATPHALPAEKRLVT
PSVVVRESTAAPNTLSNE

Sequences:

>Translated_338_residues
MATIKDVAALAGISYTTVSHVLNNTRPVSEPVRLKVEAAIAQLDYVPSAVARSLKAKTTSTIGLLIPNGMNPYFAELARG
IEDYCERNGFCVILCNSDDNPEKQRGYLRVLLEKRVDGLIVSSVGGDAGIAGGLAEVRTPLVIVDRELDGIDADMVRIDH
EQGAYLATRHLLDLGHRAIACIGGPFHSTVAEMRLAGYRRALQQASVPVRAEWALHSEFTSSAGHEAACQLLAAHPPTAI
FAGNDVIAIGVLRAAAERLIRVPQDLSVIGFDDIQMSRYVYPALTTVGQSIMQLGETAAEMLLSRIATPHALPAEKRLVT
PSVVVRESTAAPNTLSNE
>Mature_337_residues
ATIKDVAALAGISYTTVSHVLNNTRPVSEPVRLKVEAAIAQLDYVPSAVARSLKAKTTSTIGLLIPNGMNPYFAELARGI
EDYCERNGFCVILCNSDDNPEKQRGYLRVLLEKRVDGLIVSSVGGDAGIAGGLAEVRTPLVIVDRELDGIDADMVRIDHE
QGAYLATRHLLDLGHRAIACIGGPFHSTVAEMRLAGYRRALQQASVPVRAEWALHSEFTSSAGHEAACQLLAAHPPTAIF
AGNDVIAIGVLRAAAERLIRVPQDLSVIGFDDIQMSRYVYPALTTVGQSIMQLGETAAEMLLSRIATPHALPAEKRLVTP
SVVVRESTAAPNTLSNE

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=43.8438438438438, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=42.1686746987952, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=32.4324324324324, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=34.9253731343284, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=33.7313432835821, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=33.7349397590361, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=31.629392971246, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=32.9931972789116, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1787906, Length=334, Percent_Identity=28.4431137724551, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1789456, Length=330, Percent_Identity=31.2121212121212, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=28.4345047923323, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=27.6190476190476, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790715, Length=309, Percent_Identity=26.2135922330097, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=26.791277258567, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790192, Length=229, Percent_Identity=27.0742358078603, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36224; Mature: 36093

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAALAGISYTTVSHVLNNTRPVSEPVRLKVEAAIAQLDYVPSAVARSLKAKTTS
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCC
TIGLLIPNGMNPYFAELARGIEDYCERNGFCVILCNSDDNPEKQRGYLRVLLEKRVDGLI
EEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VSSVGGDAGIAGGLAEVRTPLVIVDRELDGIDADMVRIDHEQGAYLATRHLLDLGHRAIA
EECCCCCCCHHCCHHHHCCCEEEEECCCCCCCCHHEEEECCCCCHHHHHHHHHHCCCEEE
CIGGPFHSTVAEMRLAGYRRALQQASVPVRAEWALHSEFTSSAGHEAACQLLAAHPPTAI
EECCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCHHHHHHHHCCCCCEE
FAGNDVIAIGVLRAAAERLIRVPQDLSVIGFDDIQMSRYVYPALTTVGQSIMQLGETAAE
EECCCEEEHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLSRIATPHALPAEKRLVTPSVVVRESTAAPNTLSNE
HHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAALAGISYTTVSHVLNNTRPVSEPVRLKVEAAIAQLDYVPSAVARSLKAKTTS
CCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCC
TIGLLIPNGMNPYFAELARGIEDYCERNGFCVILCNSDDNPEKQRGYLRVLLEKRVDGLI
EEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VSSVGGDAGIAGGLAEVRTPLVIVDRELDGIDADMVRIDHEQGAYLATRHLLDLGHRAIA
EECCCCCCCHHCCHHHHCCCEEEEECCCCCCCCHHEEEECCCCCHHHHHHHHHHCCCEEE
CIGGPFHSTVAEMRLAGYRRALQQASVPVRAEWALHSEFTSSAGHEAACQLLAAHPPTAI
EECCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCHHHHHHHHCCCCCEE
FAGNDVIAIGVLRAAAERLIRVPQDLSVIGFDDIQMSRYVYPALTTVGQSIMQLGETAAE
EECCCEEEHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLSRIATPHALPAEKRLVTPSVVVRESTAAPNTLSNE
HHHHHHCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA