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The map label for this gene is pcaK [H]

Identifier: 66045364

GI number: 66045364

Start: 2481384

End: 2482733

Strand: Direct

Name: pcaK [H]

Synonym: Psyr_2124

Alternate gene names: 66045364

Gene position: 2481384-2482733 (Clockwise)

Preceding gene: 66045363

Following gene: 66045365

Centisome position: 40.72

GC content: 63.93

Gene sequence:

>1350_bases
ATGACGCTTTCCGCCGGCAGCCCCGCGCCGAGTACGCTCGACGTTCAGGCCTTCATCAATGCGCAACCGCTATCCGGGTA
CCAGTGGCGAGTGGTTGCGCTGTGTTTTCTGATCGTGTTTCTCGACGGCCTCGATACTGCTGCAATGGGCTTCATTGCCC
CCGCGCTCAGCCAGGATTGGGGGATTGATCGGGCCAGTCTGGGGCCGGTGATGAGCGCTGCGCTGATCGGCATGGTGTTC
GGCGCGCTGGGTTCCGGCCCGCTGGCGGACCGCTTTGGACGCAAGGTGGTGCTGGTGGTCGCGGTGCTGCTGTTCGGTCT
ATTCAGCCTGGCATCAGCCTATAGCAGCAACATCGATCAATTGCTGGTCCTGCGCCTGTTGACCGGGCTCGGGCTTGGCG
CAGCGATGCCCAACGCCACCACGTTGCTGTCGGAATACACCCCCGAACGGCTCAAGTCGTTGCTGGTGACGAGCATGTTC
TGCGGTTTCAATCTGGGCATGGCCTGCGGCGGTTTTGTGTCGGCCAAGCTGATCCCCAGCATGGGCTGGCACAGCCTGCT
GATGCTGGGCGGTATCCTGCCCCTCCTGCTGGGGTTGGTGCTGATGGTCTGGCTGCCGGAGTCGGCGCGGTTTCTGGTGG
TTCGCAATCGCGGTGCGGAGCGGATCCGCAAGGTACTGGCACCGATCGCGCCGAAGGCACTGGCCGGCATCACTGACTTC
AGTGTTCCCGAACAGAAAACGGTCAGCAGCCGCAACGTGCTGAAAGTGATTTTTTCCGGCACCTACAGCGCCGGCACGCT
GTTGCTGTGGCTGACGTATTTCATGGGCCTGGTCATCGTCTACCTGCTGACCAGCTGGCTGCCGACGCTGATGCGCGACA
GCGGCGCGAGCATGGAACAGTCGGCTTTCATCGGCGCGCTGTTTCAGTTCGGCGGCGTGTTGAGCGCTGTGGGCGTGGGC
TGGGCGATGGATCGTTACAACCCGCACAAGGTCATTGGTTGTGCCTATGCGCTGGCGGGTGTGTTCGCCTGGCTGGTCGG
GCAGAGTCTGGGTAACGTCGCGCTGTTGGCGACACTGGTGCTGCTGGCCGGTATGTGCATCAACGGCGCGCAGTCGGCGA
TGCCGTCACTGGCAGCGCGTTTTTACCCCACCCAGGGGCGTGCCACGGGGGTGTCGTGGATGCTCGGGATCGGCCGTTTC
GGCGCCATTCTCGGCGCCTGGGCCGGTGCCACGCTGCTGGGCCTGGGCTGGACGTTCGAGCAGGTGCTGACCGCGCTGGT
GATTCCGGCGGCGCTGGCCGCGATTGCGGTGCTGATCAAGGGCTGGGTCAGTCATTCGGACGCTACCTGA

Upstream 100 bases:

>100_bases
CTTCGATTTCTGAATCGCTTGTCGATTCCGAAATCAATGTGCGTGCGATTCAAGGCGCCTGCATCGGCAGGCGACCCTAT
AAAAATAAAAGGACACCTCC

Downstream 100 bases:

>100_bases
AACCTTTCAATCTGGAGTGGTGATGTGAACAGAGCGAGCAATCAACTTTTCGATGCCTACTTCATGCAACCCGAAATGCG
CGAGATCTTTTCCGATGAAG

Product: benzoate transport

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTLSAGSPAPSTLDVQAFINAQPLSGYQWRVVALCFLIVFLDGLDTAAMGFIAPALSQDWGIDRASLGPVMSAALIGMVF
GALGSGPLADRFGRKVVLVVAVLLFGLFSLASAYSSNIDQLLVLRLLTGLGLGAAMPNATTLLSEYTPERLKSLLVTSMF
CGFNLGMACGGFVSAKLIPSMGWHSLLMLGGILPLLLGLVLMVWLPESARFLVVRNRGAERIRKVLAPIAPKALAGITDF
SVPEQKTVSSRNVLKVIFSGTYSAGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQSAFIGALFQFGGVLSAVGVG
WAMDRYNPHKVIGCAYALAGVFAWLVGQSLGNVALLATLVLLAGMCINGAQSAMPSLAARFYPTQGRATGVSWMLGIGRF
GAILGAWAGATLLGLGWTFEQVLTALVIPAALAAIAVLIKGWVSHSDAT

Sequences:

>Translated_449_residues
MTLSAGSPAPSTLDVQAFINAQPLSGYQWRVVALCFLIVFLDGLDTAAMGFIAPALSQDWGIDRASLGPVMSAALIGMVF
GALGSGPLADRFGRKVVLVVAVLLFGLFSLASAYSSNIDQLLVLRLLTGLGLGAAMPNATTLLSEYTPERLKSLLVTSMF
CGFNLGMACGGFVSAKLIPSMGWHSLLMLGGILPLLLGLVLMVWLPESARFLVVRNRGAERIRKVLAPIAPKALAGITDF
SVPEQKTVSSRNVLKVIFSGTYSAGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQSAFIGALFQFGGVLSAVGVG
WAMDRYNPHKVIGCAYALAGVFAWLVGQSLGNVALLATLVLLAGMCINGAQSAMPSLAARFYPTQGRATGVSWMLGIGRF
GAILGAWAGATLLGLGWTFEQVLTALVIPAALAAIAVLIKGWVSHSDAT
>Mature_448_residues
TLSAGSPAPSTLDVQAFINAQPLSGYQWRVVALCFLIVFLDGLDTAAMGFIAPALSQDWGIDRASLGPVMSAALIGMVFG
ALGSGPLADRFGRKVVLVVAVLLFGLFSLASAYSSNIDQLLVLRLLTGLGLGAAMPNATTLLSEYTPERLKSLLVTSMFC
GFNLGMACGGFVSAKLIPSMGWHSLLMLGGILPLLLGLVLMVWLPESARFLVVRNRGAERIRKVLAPIAPKALAGITDFS
VPEQKTVSSRNVLKVIFSGTYSAGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQSAFIGALFQFGGVLSAVGVGW
AMDRYNPHKVIGCAYALAGVFAWLVGQSLGNVALLATLVLLAGMCINGAQSAMPSLAARFYPTQGRATGVSWMLGIGRFG
AILGAWAGATLLGLGWTFEQVLTALVIPAALAAIAVLIKGWVSHSDAT

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=436, Percent_Identity=26.3761467889908, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI7662270, Length=332, Percent_Identity=23.4939759036145, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI166064021, Length=229, Percent_Identity=30.1310043668122, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI4507005, Length=364, Percent_Identity=25.8241758241758, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI87081723, Length=391, Percent_Identity=35.8056265984655, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87082231, Length=417, Percent_Identity=25.1798561151079, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082159, Length=388, Percent_Identity=24.7422680412371, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789207, Length=270, Percent_Identity=26.2962962962963, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786229, Length=181, Percent_Identity=27.0718232044199, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1788074, Length=359, Percent_Identity=25.0696378830084, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788068, Length=418, Percent_Identity=22.488038277512, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082404, Length=190, Percent_Identity=25.7894736842105, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789312, Length=233, Percent_Identity=28.755364806867, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789152, Length=338, Percent_Identity=23.9644970414201, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=425, Percent_Identity=24.9411764705882, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323653, Length=266, Percent_Identity=25.9398496240602, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320595, Length=147, Percent_Identity=29.2517006802721, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922874, Length=444, Percent_Identity=27.027027027027, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640198, Length=480, Percent_Identity=24.5833333333333, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640196, Length=480, Percent_Identity=24.5833333333333, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640200, Length=480, Percent_Identity=24.5833333333333, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161077756, Length=465, Percent_Identity=22.5806451612903, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47199; Mature: 47067

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSAGSPAPSTLDVQAFINAQPLSGYQWRVVALCFLIVFLDGLDTAAMGFIAPALSQDW
CCCCCCCCCCCCEEHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GIDRASLGPVMSAALIGMVFGALGSGPLADRFGRKVVLVVAVLLFGLFSLASAYSSNIDQ
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHH
LLVLRLLTGLGLGAAMPNATTLLSEYTPERLKSLLVTSMFCGFNLGMACGGFVSAKLIPS
HHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
MGWHSLLMLGGILPLLLGLVLMVWLPESARFLVVRNRGAERIRKVLAPIAPKALAGITDF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHCCCCC
SVPEQKTVSSRNVLKVIFSGTYSAGTLLLWLTYFMGLVIVYLLTSWLPTLMRDSGASMEQ
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
SAFIGALFQFGGVLSAVGVGWAMDRYNPHKVIGCAYALAGVFAWLVGQSLGNVALLATLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LLAGMCINGAQSAMPSLAARFYPTQGRATGVSWMLGIGRFGAILGAWAGATLLGLGWTFE
HHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QVLTALVIPAALAAIAVLIKGWVSHSDAT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLSAGSPAPSTLDVQAFINAQPLSGYQWRVVALCFLIVFLDGLDTAAMGFIAPALSQDW
CCCCCCCCCCCEEHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC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HHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]