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The map label for this gene is pcaG [H]

Identifier: 66045363

GI number: 66045363

Start: 2480694

End: 2481296

Strand: Direct

Name: pcaG [H]

Synonym: Psyr_2123

Alternate gene names: 66045363

Gene position: 2480694-2481296 (Clockwise)

Preceding gene: 66045362

Following gene: 66045364

Centisome position: 40.71

GC content: 61.86

Gene sequence:

>603_bases
ATGCCCGTTCAATTGCTGCAGGAAACCCCTTCGCAAACCGCCGGCCCCTACGTCCACATCGGTCTCGCACCGCAGGTGGC
CGGCAACCCGACCCGCGAGCTGGAAATCTGGAACGAGATGGCCAGGCCCGAGGCGCCCGGCGAGCACATCGTGTTGCTGG
GCAACGTCTTCGACGGCAACGGCCATCTGATCCGCGATGCCTACCTGGAATTCTGGCAGGCGGATCATCAGGGCGCGTAT
CACAGCGAATTCCACCCGGACAGACCGTTCAACGGCTTTGGTCGCACGGCAACCGCGGACGATGGCCAGTGGATCCTGAA
GACCATCAAGCCAGGCTCTGCGCGCAATGCGGCGGGTGTGCCGATGGCGGCGCACATCAATGTTTCGCTGTTTGCGCGTG
GCATCAATATTCATCTGCAGACGCGGTTGTATTTCGACGACGAAGCCCAGGCGAATGCGCAGGACCCGGTGCTCAATCTG
ATCGAGCAGCCCCCGCGTCGCGAAACCCTGGTGGCGCGCCGCTGCACGTACGAGGGGCAGACGGCCTATCGTTTTGATAT
CCGTGTGCAGGGTGAAGGCGAGACAGTGTTCTTCGATTTCTGA

Upstream 100 bases:

>100_bases
TGGGCATGGGCAATCCAATGGATTGCCTGGCGTATCGCTTTGATATCGTGTTGCGCGGCCAGCGCAAGACTCACTTCGAA
AACTGCTGAGGAGGCCCGAC

Downstream 100 bases:

>100_bases
ATCGCTTGTCGATTCCGAAATCAATGTGCGTGCGATTCAAGGCGCCTGCATCGGCAGGCGACCCTATAAAAATAAAAGGA
CACCTCCATGACGCTTTCCG

Product: protocatechuate 3,4-dioxygenase

Products: NA

Alternate protein names: 3,4-PCD [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MPVQLLQETPSQTAGPYVHIGLAPQVAGNPTRELEIWNEMARPEAPGEHIVLLGNVFDGNGHLIRDAYLEFWQADHQGAY
HSEFHPDRPFNGFGRTATADDGQWILKTIKPGSARNAAGVPMAAHINVSLFARGINIHLQTRLYFDDEAQANAQDPVLNL
IEQPPRRETLVARRCTYEGQTAYRFDIRVQGEGETVFFDF

Sequences:

>Translated_200_residues
MPVQLLQETPSQTAGPYVHIGLAPQVAGNPTRELEIWNEMARPEAPGEHIVLLGNVFDGNGHLIRDAYLEFWQADHQGAY
HSEFHPDRPFNGFGRTATADDGQWILKTIKPGSARNAAGVPMAAHINVSLFARGINIHLQTRLYFDDEAQANAQDPVLNL
IEQPPRRETLVARRCTYEGQTAYRFDIRVQGEGETVFFDF
>Mature_199_residues
PVQLLQETPSQTAGPYVHIGLAPQVAGNPTRELEIWNEMARPEAPGEHIVLLGNVFDGNGHLIRDAYLEFWQADHQGAYH
SEFHPDRPFNGFGRTATADDGQWILKTIKPGSARNAAGVPMAAHINVSLFARGINIHLQTRLYFDDEAQANAQDPVLNLI
EQPPRRETLVARRCTYEGQTAYRFDIRVQGEGETVFFDF

Specific function: Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway [H]

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000627
- InterPro:   IPR015889
- InterPro:   IPR012786 [H]

Pfam domain/function: PF00775 Dioxygenase_C [H]

EC number: =1.13.11.3 [H]

Molecular weight: Translated: 22407; Mature: 22276

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00083 INTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVQLLQETPSQTAGPYVHIGLAPQVAGNPTRELEIWNEMARPEAPGEHIVLLGNVFDGN
CCCCHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCC
GHLIRDAYLEFWQADHQGAYHSEFHPDRPFNGFGRTATADDGQWILKTIKPGSARNAAGV
CCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
PMAAHINVSLFARGINIHLQTRLYFDDEAQANAQDPVLNLIEQPPRRETLVARRCTYEGQ
CEEEEEEEEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCC
TAYRFDIRVQGEGETVFFDF
EEEEEEEEEECCCCEEEEEC
>Mature Secondary Structure 
PVQLLQETPSQTAGPYVHIGLAPQVAGNPTRELEIWNEMARPEAPGEHIVLLGNVFDGN
CCCHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCC
GHLIRDAYLEFWQADHQGAYHSEFHPDRPFNGFGRTATADDGQWILKTIKPGSARNAAGV
CCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
PMAAHINVSLFARGINIHLQTRLYFDDEAQANAQDPVLNLIEQPPRRETLVARRCTYEGQ
CEEEEEEEEEEEEEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCC
TAYRFDIRVQGEGETVFFDF
EEEEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407791; 465136; 3194022; 7990141; 9254599; 9254600; 9298971 [H]