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The map label for this gene is pcaB [H]

Identifier: 66045365

GI number: 66045365

Start: 2482758

End: 2484119

Strand: Direct

Name: pcaB [H]

Synonym: Psyr_2125

Alternate gene names: 66045365

Gene position: 2482758-2484119 (Clockwise)

Preceding gene: 66045364

Following gene: 66045366

Centisome position: 40.74

GC content: 64.83

Gene sequence:

>1362_bases
GTGAACAGAGCGAGCAATCAACTTTTCGATGCCTACTTCATGCAACCCGAAATGCGCGAGATCTTTTCCGATGAAGGTCG
GGTGCAGGGCATGCTCGATTTCGAAGCTGCGCTGGCGCGGGCGCAGGCACGGGTCGGCCTGATCCCGCCTGAAGTGGTCG
CCGACATCGAGCTGAGCTGCGACGCACGGCTGTTCGATTTCGACGCGCTGGCAATCGCCATCGGCAGTGCCGGCAATTCG
GCGATTCCGCTGGTCAAGGCGCTGGGCAAACAGATTGCCGCACGCAGCGCTGAAGCCGAGCGCTACGTGCACATGGGCGC
GACCAGTCAGGATGTGATGGACAGCGGGCTGATCCTGCAACTGCGTCGAGCTATCGTGCTGCTTGAGCATGACCTTACCC
GACTGGCCGATGCGATGGCTGAGCAGGCGCAGCATCATGCTGGCACGCCGCTGGCCGGACGCACCTGGCTGCAGCAGGCC
ACGCCGGTCACCCTGGGGATGAAAATCGCCGGCTGGCTGGGCGCGGTCACTCGCCATCGACAGCGTTTGAACGAGATCAA
ACCGCGCCTGCTGTGCCTGCAGTTCGGCGGCGCTTCCGGCAGCCTCGCGGCGCTGGGCGATCAGGCCTTTTCGGTGGCCG
AAGCGCTGGCCGGTGAACTGCAGCTGGCGTTGCCCGAGCAGCCCTGGCACACCCAGCGCGACCGACTGGTGGAGTTTGCC
AGCCTGCTGGGCCTGATCGCCGGCAGTCTCGGCAAACTCGGGCGCGACGTGAGCCTGCTGATGCAGACCGAAGTGGGCGA
GGTCTTCGAGCCTTCGGCACCGGGCAAAGGCGGGTCTTCGACCATGCCGCACAAACGCAACCCGGTCGGCGCTGCGGTCA
TGATCAGCACCGCCACCCGCGCACCGGGGCTGGTGGCCACGATGCTGGCGGCCATGCCGCAGGAGCACGAACGCAGTCTG
GGCCTGTGGCATGCCGAGTGGGAGACCTTGCCGGAGCTGTGTTGTCTGGTCTCCGGTTCGCTGCAGCAGGCATTGCAGGT
GATACCCGGTCTGCAGGTCGATGCCGAGCGCATGGCGGTCAATCTGCAATCCACCAAAGGCCTGATTCTTGCCGAGGCAG
TGAGCATTGCGCTGGCTCAGCGCATCGGGCGCGATGCAGCCCACCATCTGGTTGAACAGTGCTGCCGGCGTGCGGTGGAG
CAGGGCGCGCATTTGCGTCAGGTGCTCGACGAAACCCCGCAGGTCAGCGAGCAGTTTTCTTCTGACGAGCTGGATCGTCT
GCTCGATCCGGCCCATTACCTTGGCCAGGCTCGCCATTGGGTCGAGCGCGCCGTGGCCGAACACACAAGGATTTCCCGAT
GA

Upstream 100 bases:

>100_bases
GCTGGTGATTCCGGCGGCGCTGGCCGCGATTGCGGTGCTGATCAAGGGCTGGGTCAGTCATTCGGACGCTACCTGAAACC
TTTCAATCTGGAGTGGTGAT

Downstream 100 bases:

>100_bases
CCGAAGACGAACGTTACGAAGCCGGCATGCAAGTGCGCCGCGCGGTGCTGGGCGACGCGCATGTCGATAACAGCCTGAGC
AAACTGACGCCGTTCAATGA

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNRASNQLFDAYFMQPEMREIFSDEGRVQGMLDFEAALARAQARVGLIPPEVVADIELSCDARLFDFDALAIAIGSAGNS
AIPLVKALGKQIAARSAEAERYVHMGATSQDVMDSGLILQLRRAIVLLEHDLTRLADAMAEQAQHHAGTPLAGRTWLQQA
TPVTLGMKIAGWLGAVTRHRQRLNEIKPRLLCLQFGGASGSLAALGDQAFSVAEALAGELQLALPEQPWHTQRDRLVEFA
SLLGLIAGSLGKLGRDVSLLMQTEVGEVFEPSAPGKGGSSTMPHKRNPVGAAVMISTATRAPGLVATMLAAMPQEHERSL
GLWHAEWETLPELCCLVSGSLQQALQVIPGLQVDAERMAVNLQSTKGLILAEAVSIALAQRIGRDAAHHLVEQCCRRAVE
QGAHLRQVLDETPQVSEQFSSDELDRLLDPAHYLGQARHWVERAVAEHTRISR

Sequences:

>Translated_453_residues
MNRASNQLFDAYFMQPEMREIFSDEGRVQGMLDFEAALARAQARVGLIPPEVVADIELSCDARLFDFDALAIAIGSAGNS
AIPLVKALGKQIAARSAEAERYVHMGATSQDVMDSGLILQLRRAIVLLEHDLTRLADAMAEQAQHHAGTPLAGRTWLQQA
TPVTLGMKIAGWLGAVTRHRQRLNEIKPRLLCLQFGGASGSLAALGDQAFSVAEALAGELQLALPEQPWHTQRDRLVEFA
SLLGLIAGSLGKLGRDVSLLMQTEVGEVFEPSAPGKGGSSTMPHKRNPVGAAVMISTATRAPGLVATMLAAMPQEHERSL
GLWHAEWETLPELCCLVSGSLQQALQVIPGLQVDAERMAVNLQSTKGLILAEAVSIALAQRIGRDAAHHLVEQCCRRAVE
QGAHLRQVLDETPQVSEQFSSDELDRLLDPAHYLGQARHWVERAVAEHTRISR
>Mature_453_residues
MNRASNQLFDAYFMQPEMREIFSDEGRVQGMLDFEAALARAQARVGLIPPEVVADIELSCDARLFDFDALAIAIGSAGNS
AIPLVKALGKQIAARSAEAERYVHMGATSQDVMDSGLILQLRRAIVLLEHDLTRLADAMAEQAQHHAGTPLAGRTWLQQA
TPVTLGMKIAGWLGAVTRHRQRLNEIKPRLLCLQFGGASGSLAALGDQAFSVAEALAGELQLALPEQPWHTQRDRLVEFA
SLLGLIAGSLGKLGRDVSLLMQTEVGEVFEPSAPGKGGSSTMPHKRNPVGAAVMISTATRAPGLVATMLAAMPQEHERSL
GLWHAEWETLPELCCLVSGSLQQALQVIPGLQVDAERMAVNLQSTKGLILAEAVSIALAQRIGRDAAHHLVEQCCRRAVE
QGAHLRQVLDETPQVSEQFSSDELDRLLDPAHYLGQARHWVERAVAEHTRISR

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=456, Percent_Identity=26.5350877192982, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI183227688, Length=321, Percent_Identity=27.1028037383178, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI31541964, Length=328, Percent_Identity=27.7439024390244, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI68303542, Length=328, Percent_Identity=27.7439024390244, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=373, Percent_Identity=23.8605898123324, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324993, Length=363, Percent_Identity=24.7933884297521, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24647570, Length=292, Percent_Identity=28.0821917808219, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221473854, Length=401, Percent_Identity=23.9401496259352, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706858, Length=401, Percent_Identity=23.9401496259352, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78710009, Length=266, Percent_Identity=26.6917293233083, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 49076; Mature: 49076

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRASNQLFDAYFMQPEMREIFSDEGRVQGMLDFEAALARAQARVGLIPPEVVADIELSC
CCCCHHHHHHHHHCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
DARLFDFDALAIAIGSAGNSAIPLVKALGKQIAARSAEAERYVHMGATSQDVMDSGLILQ
CCEEEEHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHH
LRRAIVLLEHDLTRLADAMAEQAQHHAGTPLAGRTWLQQATPVTLGMKIAGWLGAVTRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QRLNEIKPRLLCLQFGGASGSLAALGDQAFSVAEALAGELQLALPEQPWHTQRDRLVEFA
HHHHHCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
SLLGLIAGSLGKLGRDVSLLMQTEVGEVFEPSAPGKGGSSTMPHKRNPVGAAVMISTATR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
APGLVATMLAAMPQEHERSLGLWHAEWETLPELCCLVSGSLQQALQVIPGLQVDAERMAV
CCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEE
NLQSTKGLILAEAVSIALAQRIGRDAAHHLVEQCCRRAVEQGAHLRQVLDETPQVSEQFS
EHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCC
SDELDRLLDPAHYLGQARHWVERAVAEHTRISR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRASNQLFDAYFMQPEMREIFSDEGRVQGMLDFEAALARAQARVGLIPPEVVADIELSC
CCCCHHHHHHHHHCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
DARLFDFDALAIAIGSAGNSAIPLVKALGKQIAARSAEAERYVHMGATSQDVMDSGLILQ
CCEEEEHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCHHHHH
LRRAIVLLEHDLTRLADAMAEQAQHHAGTPLAGRTWLQQATPVTLGMKIAGWLGAVTRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QRLNEIKPRLLCLQFGGASGSLAALGDQAFSVAEALAGELQLALPEQPWHTQRDRLVEFA
HHHHHCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
SLLGLIAGSLGKLGRDVSLLMQTEVGEVFEPSAPGKGGSSTMPHKRNPVGAAVMISTATR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
APGLVATMLAAMPQEHERSLGLWHAEWETLPELCCLVSGSLQQALQVIPGLQVDAERMAV
CCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEE
NLQSTKGLILAEAVSIALAQRIGRDAAHHLVEQCCRRAVEQGAHLRQVLDETPQVSEQFS
EHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCC
SDELDRLLDPAHYLGQARHWVERAVAEHTRISR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]