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The map label for this gene is oprF [H]

Identifier: 66045337

GI number: 66045337

Start: 2444715

End: 2445749

Strand: Direct

Name: oprF [H]

Synonym: Psyr_2097

Alternate gene names: 66045337

Gene position: 2444715-2445749 (Clockwise)

Preceding gene: 66045336

Following gene: 66045343

Centisome position: 40.12

GC content: 56.23

Gene sequence:

>1035_bases
ATGAAACTGAAAAACACCTTGGGCTTGGCCATTGGTACTATTGTTGCCGCAACTTCGTTCGGCGCGCTGGCTCAAGGCCA
AGGCGCGGTCGAAATCGAAGGCTTCGCCAAGAAAGAGTACTACGACAGCGATCGTAACTTCAAAAACGACGGCAACCTGT
TCGGCGGCTCCGTTGGCTACTTCCTGACTGACGATGTTGAGCTGCGTCTGGGCTACGACGAAGTCCACAACGTTCGTAGC
GACAGCGGCAAGAACATCAAGGGCGCAGACACTGCTCTGGACGCTCTGTACCACTTCAACAACCCAGGCGACATGCTGCG
TCCATACGTATCTGCCGGCTTCTCTGACCAGAGCATCGGTCAAGACGCTCGCCGTGGCCGTGACGGTTCCACCTTCGCCA
ACATCGGCGGCGGCGCCAAGCTGTACTTCACTGACAACTTCTATGCCCGTGCAGGCGTTGAAGCTCAGTACAACATCGAC
CAAGGCAACACCGAGTGGGCGCCAAGCGTCGGTATCGGTGTAAACTTCGGCGGCGGCAGCAAGAAAGTTGAAGCGGCTCC
AGCTCCAGTAGCTGAAGTGTGCTCCGACAGCGACAACGACGGCGTGTGCGACAACGTCGACAAGTGCCCGGACACCCCAG
CCAACGTTACCGTTGACGCTGATGGCTGCCCAGCAGTTGCCGAAGTGGTTCGTGTTGAGCTGGACGTGAAGTTCGACTTC
GACAAATCCGTAGTCAAGCCTAGCAGCTACGGCGACATCAAGAACCTCGCTGACTTCATGCAGCAGTACCCACAGACCAC
CACCACTGTTGAAGGTCACACTGACTCCGTCGGTCCTGACGCTTACAACCAAAAACTGTCCGAGCGTCGTGCAAACGCCG
TTAAACAGGTTCTGGTTAACCAGTACGGTGTTGGCGCTAGCCGCGTAAACTCGGTTGGTTACGGCGAAACCAAGCCAGTT
GCTGACAACGCAACTGAAGCAGGTCGCGCAGTTAACCGTCGCGTAGAAGCAGAAGTAGAAGCTCAAGCTAAGTAA

Upstream 100 bases:

>100_bases
TGCAAATATCTCTAACAAACTGGCAAGGTCTGGTAGACTTGCCGCTGAGTTGTCCCCCTGTGTGGGACTGCTTAATAATC
ATCAGATGGGGATTTAACGG

Downstream 100 bases:

>100_bases
TTAGCCGCTTGTACTGAAAAGCCCGGCTCAGGCCGGGCTTTTCTTTGCCTGCGATTTGGCAGGGTGCTTGTTCAGACGGG
CCTGGTGTCAGCTGGCATTC

Product: thrombospondin type 3 repeat-containing OmpA/MotB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKLKNTLGLAIGTIVAATSFGALAQGQGAVEIEGFAKKEYYDSDRNFKNDGNLFGGSVGYFLTDDVELRLGYDEVHNVRS
DSGKNIKGADTALDALYHFNNPGDMLRPYVSAGFSDQSIGQDARRGRDGSTFANIGGGAKLYFTDNFYARAGVEAQYNID
QGNTEWAPSVGIGVNFGGGSKKVEAAPAPVAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
DKSVVKPSSYGDIKNLADFMQQYPQTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLVNQYGVGASRVNSVGYGETKPV
ADNATEAGRAVNRRVEAEVEAQAK

Sequences:

>Translated_344_residues
MKLKNTLGLAIGTIVAATSFGALAQGQGAVEIEGFAKKEYYDSDRNFKNDGNLFGGSVGYFLTDDVELRLGYDEVHNVRS
DSGKNIKGADTALDALYHFNNPGDMLRPYVSAGFSDQSIGQDARRGRDGSTFANIGGGAKLYFTDNFYARAGVEAQYNID
QGNTEWAPSVGIGVNFGGGSKKVEAAPAPVAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
DKSVVKPSSYGDIKNLADFMQQYPQTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLVNQYGVGASRVNSVGYGETKPV
ADNATEAGRAVNRRVEAEVEAQAK
>Mature_344_residues
MKLKNTLGLAIGTIVAATSFGALAQGQGAVEIEGFAKKEYYDSDRNFKNDGNLFGGSVGYFLTDDVELRLGYDEVHNVRS
DSGKNIKGADTALDALYHFNNPGDMLRPYVSAGFSDQSIGQDARRGRDGSTFANIGGGAKLYFTDNFYARAGVEAQYNID
QGNTEWAPSVGIGVNFGGGSKKVEAAPAPVAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
DKSVVKPSSYGDIKNLADFMQQYPQTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLVNQYGVGASRVNSVGYGETKPV
ADNATEAGRAVNRRVEAEVEAQAK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=130, Percent_Identity=36.1538461538462, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=40.4040404040404, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787191, Length=117, Percent_Identity=35.8974358974359, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 36619; Mature: 36619

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKNTLGLAIGTIVAATSFGALAQGQGAVEIEGFAKKEYYDSDRNFKNDGNLFGGSVGY
CCCCHHHHHHHHHHHHHHHHCHHHCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCEECE
FLTDDVELRLGYDEVHNVRSDSGKNIKGADTALDALYHFNNPGDMLRPYVSAGFSDQSIG
EEECCEEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHCC
QDARRGRDGSTFANIGGGAKLYFTDNFYARAGVEAQYNIDQGNTEWAPSVGIGVNFGGGS
HHHHCCCCCCCEEECCCCEEEEEECCEEEECCCCEEEECCCCCCCCCCCCCEEEEECCCC
KKVEAAPAPVAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
CEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHEEEEEEEEECC
DKSVVKPSSYGDIKNLADFMQQYPQTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLVN
CCHHCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QYGVGASRVNSVGYGETKPVADNATEAGRAVNRRVEAEVEAQAK
HCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLKNTLGLAIGTIVAATSFGALAQGQGAVEIEGFAKKEYYDSDRNFKNDGNLFGGSVGY
CCCCHHHHHHHHHHHHHHHHCHHHCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCEECE
FLTDDVELRLGYDEVHNVRSDSGKNIKGADTALDALYHFNNPGDMLRPYVSAGFSDQSIG
EEECCEEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHCC
QDARRGRDGSTFANIGGGAKLYFTDNFYARAGVEAQYNIDQGNTEWAPSVGIGVNFGGGS
HHHHCCCCCCCEEECCCCEEEEEECCEEEECCCCEEEECCCCCCCCCCCCCEEEEECCCC
KKVEAAPAPVAEVCSDSDNDGVCDNVDKCPDTPANVTVDADGCPAVAEVVRVELDVKFDF
CEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHEEEEEEEEECC
DKSVVKPSSYGDIKNLADFMQQYPQTTTTVEGHTDSVGPDAYNQKLSERRANAVKQVLVN
CCHHCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QYGVGASRVNSVGYGETKPVADNATEAGRAVNRRVEAEVEAQAK
HCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]