Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 66045325

GI number: 66045325

Start: 2430393

End: 2431286

Strand: Direct

Name: prpB [H]

Synonym: Psyr_2085

Alternate gene names: 66045325

Gene position: 2430393-2431286 (Clockwise)

Preceding gene: 66045324

Following gene: 66045326

Centisome position: 39.88

GC content: 60.29

Gene sequence:

>894_bases
ATGAGTCATGACAACAGCACTCCCGGCCAGCGTTTCCGCGATGCAGTTACCAGCGAGCAGCCGCTGCAAGTGGTCGGCGC
GATCAATGCCAACCACGCACTGCTCGCCAGGCGTGCCGGGTTCAAGGCTATTTATCTGTCGGGTGGCGGAGTCGCTGCAG
GATCTCTGGGCATTCCGGACCTGGGAATCACTGGGCTTGAAGACGTACTGATCGATGTGCGGCGCATCACCGACGTCTGC
GATCTGCCGTTGCTGGTGGACGTCGACACCGGTTTCGGCTCTTCGGCTTTCAATGTGGCGCGTACCGTGCGCTCGATGAT
CAAGGCCGGGGCGGCGGCGATTCACATCGAGGATCAGGTGGGCGCCAAGCGTTGCGGGCACCGTCCCAACAAGGAAATCG
TTTCTCAGCAGGAAATGGTCGACCGGATCAAGGCTGCCGTGGATGCCCGGACTGACGACAGCTTCGTGATCATGGCGCGT
ACCGATGCGCTGGCGGTTGAAGGGCTGGAGTCGGCGCTGGAGCGTGCCGCTGCCTGTATTGAAGCCGGTGCGGATATGGT
GTTTCCAGAGGCCATCACCGAACTGGCGATGTACAAGCAATTCGCCAACCGCGCAGGCGTGCCGATCCTCGCCAACATTA
CCGAGTTCGGCGCCACGCCGTTGTTTACGGTCGATGAGCTGCGCGAGGCGGACGTCTCGCTGGTGCTGTATCCGCTTTCC
GCATTCCGCGCGATGAACAAGGCTGCAGAAAATGTCTACGGCGCAATCCGCCGTGACGGCAGCCAGAAAAACGTGATTGA
CAGCATGCAGACGCGCATGGAGCTGTATGACGCGATCGATTACCACACATTCGAACAGAAGCTGGATGCGCTGTTTGCGC
AGAAGAAGGGGTGA

Upstream 100 bases:

>100_bases
TGGCCGAATTGTTGATGCGCCGCCATATCGGCGCGTCCAGACGCAACATCGAACGCCACTATCAGGCGGCTTCCCAGACG
ACTTCCGACCGAGGTAAACA

Downstream 100 bases:

>100_bases
TGCCTGTGAAATGCCGGGCTTCTGGCCGCAGGCCGTAGGAGCGAACTTGTTCGCGAAAGGGTTGTTATTCTCTGATAATG
CAACGTTGGAATCACTATCT

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSHDNSTPGQRFRDAVTSEQPLQVVGAINANHALLARRAGFKAIYLSGGGVAAGSLGIPDLGITGLEDVLIDVRRITDVC
DLPLLVDVDTGFGSSAFNVARTVRSMIKAGAAAIHIEDQVGAKRCGHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMAR
TDALAVEGLESALERAAACIEAGADMVFPEAITELAMYKQFANRAGVPILANITEFGATPLFTVDELREADVSLVLYPLS
AFRAMNKAAENVYGAIRRDGSQKNVIDSMQTRMELYDAIDYHTFEQKLDALFAQKKG

Sequences:

>Translated_297_residues
MSHDNSTPGQRFRDAVTSEQPLQVVGAINANHALLARRAGFKAIYLSGGGVAAGSLGIPDLGITGLEDVLIDVRRITDVC
DLPLLVDVDTGFGSSAFNVARTVRSMIKAGAAAIHIEDQVGAKRCGHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMAR
TDALAVEGLESALERAAACIEAGADMVFPEAITELAMYKQFANRAGVPILANITEFGATPLFTVDELREADVSLVLYPLS
AFRAMNKAAENVYGAIRRDGSQKNVIDSMQTRMELYDAIDYHTFEQKLDALFAQKKG
>Mature_296_residues
SHDNSTPGQRFRDAVTSEQPLQVVGAINANHALLARRAGFKAIYLSGGGVAAGSLGIPDLGITGLEDVLIDVRRITDVCD
LPLLVDVDTGFGSSAFNVARTVRSMIKAGAAAIHIEDQVGAKRCGHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMART
DALAVEGLESALERAAACIEAGADMVFPEAITELAMYKQFANRAGVPILANITEFGATPLFTVDELREADVSLVLYPLSA
FRAMNKAAENVYGAIRRDGSQKNVIDSMQTRMELYDAIDYHTFEQKLDALFAQKKG

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=290, Percent_Identity=75.1724137931034, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790445, Length=256, Percent_Identity=27.734375, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982926, Length=186, Percent_Identity=30.6451612903226, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17561814, Length=184, Percent_Identity=30.9782608695652, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32018; Mature: 31887

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDNSTPGQRFRDAVTSEQPLQVVGAINANHALLARRAGFKAIYLSGGGVAAGSLGIPD
CCCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCEEEEEECCCEEECCCCCCC
LGITGLEDVLIDVRRITDVCDLPLLVDVDTGFGSSAFNVARTVRSMIKAGAAAIHIEDQV
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECHHH
GAKRCGHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMARTDALAVEGLESALERAAACI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMVFPEAITELAMYKQFANRAGVPILANITEFGATPLFTVDELREADVSLVLYPLS
HHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCCEEHHHHHHCCHHEEEHHHH
AFRAMNKAAENVYGAIRRDGSQKNVIDSMQTRMELYDAIDYHTFEQKLDALFAQKKG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHDNSTPGQRFRDAVTSEQPLQVVGAINANHALLARRAGFKAIYLSGGGVAAGSLGIPD
CCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCEEEEEECCCEEECCCCCCC
LGITGLEDVLIDVRRITDVCDLPLLVDVDTGFGSSAFNVARTVRSMIKAGAAAIHIEDQV
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECHHH
GAKRCGHRPNKEIVSQQEMVDRIKAAVDARTDDSFVIMARTDALAVEGLESALERAAACI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMVFPEAITELAMYKQFANRAGVPILANITEFGATPLFTVDELREADVSLVLYPLS
HHCCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCCEEHHHHHHCCHHEEEHHHH
AFRAMNKAAENVYGAIRRDGSQKNVIDSMQTRMELYDAIDYHTFEQKLDALFAQKKG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9006051; 11677609; 10482501; 14575713 [H]