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The map label for this gene is prpC [H]

Identifier: 66045326

GI number: 66045326

Start: 2431446

End: 2432573

Strand: Direct

Name: prpC [H]

Synonym: Psyr_2086

Alternate gene names: 66045326

Gene position: 2431446-2432573 (Clockwise)

Preceding gene: 66045325

Following gene: 66045327

Centisome position: 39.9

GC content: 61.7

Gene sequence:

>1128_bases
ATGGCTGAAGCAAAAGTATTGAGTGGTGCCGGGCTGCGCGGGCAGGTTGCCGGGCAGACGGCGTTGTCCACGGTGGGCAT
GGCCGGTGCCGGGCTGACCTATCGCGGCTATGACGTGCGCGATCTGGCTGCCGAGGCGCGTTTTGAAGAAGTGGCCTACC
TGCTGCTGTACGGCGAACTGCCCAATCAGGCCGAATTGTCTGCCTATATGGACAAACTCAAGCGCCTGCGCGATCTGCCG
CAGGCCCTGAAGGAAGTGCTGGAGCGCATACCCGCCGACACTCATCCGATGGATGTCATGCGCACCGGTGCTTCCATGCT
CGGCACCCTGGAGCCGGAGCGCAGTTTCGATCAGCAGCGCGACTCGACCGGCCGTTTGCTCGCGGCGTTCCCGGCGATCA
TGTGCTACTGGTATCGCTTCAGTCACGACGGCAGGCGTATCGACTGCACCTCGGCCGAAGACTCCATCGGCGGGCATTTT
CTGCACCTGCTGCTGGACAAGGCGCCCAGCGAACTGCACCGCAAGGTCATGGACGTATCGCTGATTCTCTACGCCGAACA
CGAATTCAATGCCTCGACATTCACTGCCCGCGTCTGCGCGTCGACCCTGTCGGACCTGTATTCCTGCGTCACGGCAGCCA
TCGGCACCTTGCGCGGCCCGCTGCACGGCGGTGCCAACGAGGCTGCAATGGAAATGATTCAGCGCTTCGCCTCGGCTGAA
GAGGCAACCAAAGGCACGCTGGGCATGCTGGAGCGCAAGGAAAAGATCATGGGCTTTGGCCATGCGATCTACAAGGAGTC
CGACCCGCGCAACGAGGTGATCAAGGGCTGGTCGAAGAAACTCGCCGACGAAGTGGGCGATACGGTGCTGTTCCCGGTTT
CCGAGGCCATCGACAAGGTCATGTGGGAGCAGAAGAAACTGTTCCCCAACGCCGACTTCTACCATGCGTCGGCGTATCAC
TTCATGGGCATCCCGACCAAGCTGTTTACGCCGATCTTCGTCTGCTCACGCCTGACCGGCTGGGCCGCGCACGTTTTCGA
GCAGCGCGCCAACAACCGCATCATTCGCCCGAGCGCCGAATACATCGGCGTCGAACAGCGCGCGTTCGTACCCCTGGAAC
AGCGCTAA

Upstream 100 bases:

>100_bases
GGTTGTTATTCTCTGATAATGCAACGTTGGAATCACTATCTTCGCTAACAAGAGAAGCCTCGCTCCGTCCCATCACACAA
GAACAAAATTGGAGAACACC

Downstream 100 bases:

>100_bases
AAGGGGGCAGGGGAGCCGCCGACGAGCTCCCCACCCGTATTCCGAACACTCCGTGATCGAGTCCCGCAATGAACAGTGAA
TTTCGCAAACCGCTCCCTGG

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MAEAKVLSGAGLRGQVAGQTALSTVGMAGAGLTYRGYDVRDLAAEARFEEVAYLLLYGELPNQAELSAYMDKLKRLRDLP
QALKEVLERIPADTHPMDVMRTGASMLGTLEPERSFDQQRDSTGRLLAAFPAIMCYWYRFSHDGRRIDCTSAEDSIGGHF
LHLLLDKAPSELHRKVMDVSLILYAEHEFNASTFTARVCASTLSDLYSCVTAAIGTLRGPLHGGANEAAMEMIQRFASAE
EATKGTLGMLERKEKIMGFGHAIYKESDPRNEVIKGWSKKLADEVGDTVLFPVSEAIDKVMWEQKKLFPNADFYHASAYH
FMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAEYIGVEQRAFVPLEQR

Sequences:

>Translated_375_residues
MAEAKVLSGAGLRGQVAGQTALSTVGMAGAGLTYRGYDVRDLAAEARFEEVAYLLLYGELPNQAELSAYMDKLKRLRDLP
QALKEVLERIPADTHPMDVMRTGASMLGTLEPERSFDQQRDSTGRLLAAFPAIMCYWYRFSHDGRRIDCTSAEDSIGGHF
LHLLLDKAPSELHRKVMDVSLILYAEHEFNASTFTARVCASTLSDLYSCVTAAIGTLRGPLHGGANEAAMEMIQRFASAE
EATKGTLGMLERKEKIMGFGHAIYKESDPRNEVIKGWSKKLADEVGDTVLFPVSEAIDKVMWEQKKLFPNADFYHASAYH
FMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAEYIGVEQRAFVPLEQR
>Mature_374_residues
AEAKVLSGAGLRGQVAGQTALSTVGMAGAGLTYRGYDVRDLAAEARFEEVAYLLLYGELPNQAELSAYMDKLKRLRDLPQ
ALKEVLERIPADTHPMDVMRTGASMLGTLEPERSFDQQRDSTGRLLAAFPAIMCYWYRFSHDGRRIDCTSAEDSIGGHFL
HLLLDKAPSELHRKVMDVSLILYAEHEFNASTFTARVCASTLSDLYSCVTAAIGTLRGPLHGGANEAAMEMIQRFASAEE
ATKGTLGMLERKEKIMGFGHAIYKESDPRNEVIKGWSKKLADEVGDTVLFPVSEAIDKVMWEQKKLFPNADFYHASAYHF
MGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAEYIGVEQRAFVPLEQR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=377, Percent_Identity=22.5464190981432, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1786527, Length=367, Percent_Identity=52.8610354223433, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1786939, Length=359, Percent_Identity=32.8690807799443, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=354, Percent_Identity=22.8813559322034, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319850, Length=392, Percent_Identity=24.7448979591837, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324328, Length=380, Percent_Identity=23.6842105263158, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640124, Length=378, Percent_Identity=24.0740740740741, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640126, Length=378, Percent_Identity=24.0740740740741, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356863, Length=418, Percent_Identity=22.488038277512, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 41738; Mature: 41607

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAKVLSGAGLRGQVAGQTALSTVGMAGAGLTYRGYDVRDLAAEARFEEVAYLLLYGEL
CCCCCEECCCCCCCCCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHEEECCC
PNQAELSAYMDKLKRLRDLPQALKEVLERIPADTHPMDVMRTGASMLGTLEPERSFDQQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHC
DSTGRLLAAFPAIMCYWYRFSHDGRRIDCTSAEDSIGGHFLHLLLDKAPSELHRKVMDVS
CCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
LILYAEHEFNASTFTARVCASTLSDLYSCVTAAIGTLRGPLHGGANEAAMEMIQRFASAE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
EATKGTLGMLERKEKIMGFGHAIYKESDPRNEVIKGWSKKLADEVGDTVLFPVSEAIDKV
HHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECHHHHHHHH
MWEQKKLFPNADFYHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAE
HHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
YIGVEQRAFVPLEQR
HHCCCCCCCCCCCCC
>Mature Secondary Structure 
AEAKVLSGAGLRGQVAGQTALSTVGMAGAGLTYRGYDVRDLAAEARFEEVAYLLLYGEL
CCCCEECCCCCCCCCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHEEECCC
PNQAELSAYMDKLKRLRDLPQALKEVLERIPADTHPMDVMRTGASMLGTLEPERSFDQQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHC
DSTGRLLAAFPAIMCYWYRFSHDGRRIDCTSAEDSIGGHFLHLLLDKAPSELHRKVMDVS
CCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHH
LILYAEHEFNASTFTARVCASTLSDLYSCVTAAIGTLRGPLHGGANEAAMEMIQRFASAE
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
EATKGTLGMLERKEKIMGFGHAIYKESDPRNEVIKGWSKKLADEVGDTVLFPVSEAIDKV
HHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEECHHHHHHHH
MWEQKKLFPNADFYHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAE
HHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHH
YIGVEQRAFVPLEQR
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]