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The map label for this gene is nth [H]

Identifier: 62185498

GI number: 62185498

Start: 1049558

End: 1050217

Strand: Direct

Name: nth [H]

Synonym: CAB898

Alternate gene names: 62185498

Gene position: 1049558-1050217 (Clockwise)

Preceding gene: 62185497

Following gene: 62185499

Centisome position: 91.71

GC content: 41.21

Gene sequence:

>660_bases
TTGGGAAATAGAGATTGCATTAAAAATTTTATTCTTTCTACTTTGAACGAGCTCTTCCCCGATCCACAACCGTCGTTAAC
AGGGTGGGAAACTCCGTTTCAGCTCCTTGTGGCTATTGTACTTTCAGGAAATTCCACCGATAAAGCTGTAAATGCTGTCA
CCCCAAGGTTGTTCTCCCTTGCTCCTGATGCGCAGGCTTTAGTGCAACTCCCCTTAGAAGACTTATACTTCATCATCTCG
CCTTGCGGCCTCGGCAGAAGAAAAGCTGCCTACTTACATCATTTAGCACAGATATTGTTAGAAAAATATCATGGGGAACC
TCCGGCTTCTCTAGAGCTATTAACACAATTGCCCGGAGTGGGAAGGAAAACTGCATCGGTTTTCTTAGGAATTATTTATA
AAATACCGACCTTTCCTGTAGATACACATATCTTAAGATTATCCCAACGTTGGGGAATCTCTAATAAACGCAGTCCTTCA
GCAGCAGAAAAAGATCTCGTTCTTTTTTTCGGAGAAGCTAACTCTCCAAAACTCCATTTACAACTCATCTACTACGCCAG
AGAGTACTGCCCTGCTCTCTACCACGACACAAATAAATGCAAAATATGTGCATATTTAATCCGAAGATCTTATGAAAAAA
AAACAAACAAAACCCTCTAA

Upstream 100 bases:

>100_bases
ATTGCTTTAGAACTAAGACAAGCTCTGCAGACTACAGGAAATCTTTCTGGATCTGAAATTAATGAAACCATACTGGGAGA
AATTTTTAGCAGGTTTTGTA

Downstream 100 bases:

>100_bases
GAAATAATATGTTCTGTTTTTAAAAATAGAAATAGTTTCATATAAAGTTTTTCCTCTGTATATGTCATGGATGAAATTTA
CTTTGCTTTCTAACGTAGAA

Product: putative DNA repair protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MGNRDCIKNFILSTLNELFPDPQPSLTGWETPFQLLVAIVLSGNSTDKAVNAVTPRLFSLAPDAQALVQLPLEDLYFIIS
PCGLGRRKAAYLHHLAQILLEKYHGEPPASLELLTQLPGVGRKTASVFLGIIYKIPTFPVDTHILRLSQRWGISNKRSPS
AAEKDLVLFFGEANSPKLHLQLIYYAREYCPALYHDTNKCKICAYLIRRSYEKKTNKTL

Sequences:

>Translated_219_residues
MGNRDCIKNFILSTLNELFPDPQPSLTGWETPFQLLVAIVLSGNSTDKAVNAVTPRLFSLAPDAQALVQLPLEDLYFIIS
PCGLGRRKAAYLHHLAQILLEKYHGEPPASLELLTQLPGVGRKTASVFLGIIYKIPTFPVDTHILRLSQRWGISNKRSPS
AAEKDLVLFFGEANSPKLHLQLIYYAREYCPALYHDTNKCKICAYLIRRSYEKKTNKTL
>Mature_218_residues
GNRDCIKNFILSTLNELFPDPQPSLTGWETPFQLLVAIVLSGNSTDKAVNAVTPRLFSLAPDAQALVQLPLEDLYFIISP
CGLGRRKAAYLHHLAQILLEKYHGEPPASLELLTQLPGVGRKTASVFLGIIYKIPTFPVDTHILRLSQRWGISNKRSPSA
AEKDLVLFFGEANSPKLHLQLIYYAREYCPALYHDTNKCKICAYLIRRSYEKKTNKTL

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=171, Percent_Identity=25.1461988304094, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787920, Length=198, Percent_Identity=34.8484848484849, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17554540, Length=182, Percent_Identity=29.6703296703297, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550361, Length=121, Percent_Identity=33.0578512396694, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24626; Mature: 24495

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRDCIKNFILSTLNELFPDPQPSLTGWETPFQLLVAIVLSGNSTDKAVNAVTPRLFSL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCHHHHHC
APDAQALVQLPLEDLYFIISPCGLGRRKAAYLHHLAQILLEKYHGEPPASLELLTQLPGV
CCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
GRKTASVFLGIIYKIPTFPVDTHILRLSQRWGISNKRSPSAAEKDLVLFFGEANSPKLHL
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
QLIYYAREYCPALYHDTNKCKICAYLIRRSYEKKTNKTL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GNRDCIKNFILSTLNELFPDPQPSLTGWETPFQLLVAIVLSGNSTDKAVNAVTPRLFSL
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCHHHHHC
APDAQALVQLPLEDLYFIISPCGLGRRKAAYLHHLAQILLEKYHGEPPASLELLTQLPGV
CCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
GRKTASVFLGIIYKIPTFPVDTHILRLSQRWGISNKRSPSAAEKDLVLFFGEANSPKLHL
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
QLIYYAREYCPALYHDTNKCKICAYLIRRSYEKKTNKTL
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]