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The map label for this gene is trmE

Identifier: 62185497

GI number: 62185497

Start: 1048237

End: 1049568

Strand: Direct

Name: trmE

Synonym: CAB897

Alternate gene names: 62185497

Gene position: 1048237-1049568 (Clockwise)

Preceding gene: 62185493

Following gene: 62185498

Centisome position: 91.6

GC content: 43.77

Gene sequence:

>1332_bases
ATGATTAAAAACGATACTATTGCTGCGATAGCCACACCTCCCGGAGAGGGAAGTATTGCCATTGTCCGTATATCGGGCCC
AGAAGCTATTCAGATCACAGACAAAATTTTCTCCGGATCCGTACCGTCGTTTTCTTCCCACACAGCACATCTAGGTACGG
TAAGTTACAATGGCCAGCAGATCGATCAAACCCTTTTATTAATTATGCGAGCTCCGCGTTCATTCACGGGAGAAGATGTT
GTCGAACTCCAGTGTCATGGAGGCTATTTTTCCTGCTCTCAAATCTTAGCAGCTTTGATAGCCGAAGGAGCTCGCCCTGC
TCTCCCTGGAGAATTTTCTCAACGCGCTTTTCTCAATGGAAAAATTGATCTTATCCAAGCAGAAGCAATTCAAAATATTA
TTGCTGCTGATAATTTAGACGCTTTCCATATAGCGCAGAACCATTTCCAGGGACACTTCTCAAAGAAAGTTCAACAGATT
TCTTCGTTGATTATCGAATCTCTCGCCTTTATTGAAGTTCTTGCAGATTTCCCTGAAGAAGAACAGCCGGATATGCAAGA
ACCGATGAACCGACTCCACGAGGCGATATTGATCATCGACGATCTCATTGCTAGTTTTGATCAAGGGCAAAGGTTAGCTC
AAGGAACGAGTATTGTATTAGCCGGCCACCCTAATGCAGGAAAATCTTCTCTTCTTAACGCACTCACGAATAAAAATCGC
GCCATTGTTACGGATATCCCCGGAACTACAAGAGATATATTAGAAGAAAATTGGATGTTACAAGGAAAACGTATCCGACT
TATCGACTCTGCAGGACAGCGAGAAACCCATAATCCTATAGAACAAGAAGGTATTGAGCGGGCCATAGCTGCCATGGAAG
CGTCCGAAGCCATTCTTTGGGTTATGGATGCCTCACAACCTCCACCACCTCTTCCAGAAATCTTAATGCGCAAACCTTCC
CTTCTTCTTTGGAACAAATCCGACCTAGCGACACCACCTCATCTTGAAACCACCTTACCTCAATTAGCTGTCTCCGCAAA
AACAGGCGCAGGCATGATTGAGCTCAAACAATTTATCCAACAATGGATGCAAAAACAACAGTTGGGCAAGAATGGAAAAG
TCTTTCTTATTTCTTCGCGACACCATACAATACTGCAACACATGCGCACCTATTTGCTCTCAGCCCAGGAAGGATTACAG
AGTCAATCCCCCCCGGAGTTTATTGCTTTAGAACTAAGACAAGCTCTGCAGACTACAGGAAATCTTTCTGGATCTGAAAT
TAATGAAACCATACTGGGAGAAATTTTTAGCAGGTTTTGTATTGGGAAATAG

Upstream 100 bases:

>100_bases
CCAAACTAATTAAATAGAAATCGTTAAAAAACCTGCCTACATCTTTAATGGGGAACCTGATATCCTAACTCTCAAGTTAG
TTTTATTTTCATCCATTTTC

Downstream 100 bases:

>100_bases
AGATTGCATTAAAAATTTTATTCTTTCTACTTTGAACGAGCTCTTCCCCGATCCACAACCGTCGTTAACAGGGTGGGAAA
CTCCGTTTCAGCTCCTTGTG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIKNDTIAAIATPPGEGSIAIVRISGPEAIQITDKIFSGSVPSFSSHTAHLGTVSYNGQQIDQTLLLIMRAPRSFTGEDV
VELQCHGGYFSCSQILAALIAEGARPALPGEFSQRAFLNGKIDLIQAEAIQNIIAADNLDAFHIAQNHFQGHFSKKVQQI
SSLIIESLAFIEVLADFPEEEQPDMQEPMNRLHEAILIIDDLIASFDQGQRLAQGTSIVLAGHPNAGKSSLLNALTNKNR
AIVTDIPGTTRDILEENWMLQGKRIRLIDSAGQRETHNPIEQEGIERAIAAMEASEAILWVMDASQPPPPLPEILMRKPS
LLLWNKSDLATPPHLETTLPQLAVSAKTGAGMIELKQFIQQWMQKQQLGKNGKVFLISSRHHTILQHMRTYLLSAQEGLQ
SQSPPEFIALELRQALQTTGNLSGSEINETILGEIFSRFCIGK

Sequences:

>Translated_443_residues
MIKNDTIAAIATPPGEGSIAIVRISGPEAIQITDKIFSGSVPSFSSHTAHLGTVSYNGQQIDQTLLLIMRAPRSFTGEDV
VELQCHGGYFSCSQILAALIAEGARPALPGEFSQRAFLNGKIDLIQAEAIQNIIAADNLDAFHIAQNHFQGHFSKKVQQI
SSLIIESLAFIEVLADFPEEEQPDMQEPMNRLHEAILIIDDLIASFDQGQRLAQGTSIVLAGHPNAGKSSLLNALTNKNR
AIVTDIPGTTRDILEENWMLQGKRIRLIDSAGQRETHNPIEQEGIERAIAAMEASEAILWVMDASQPPPPLPEILMRKPS
LLLWNKSDLATPPHLETTLPQLAVSAKTGAGMIELKQFIQQWMQKQQLGKNGKVFLISSRHHTILQHMRTYLLSAQEGLQ
SQSPPEFIALELRQALQTTGNLSGSEINETILGEIFSRFCIGK
>Mature_443_residues
MIKNDTIAAIATPPGEGSIAIVRISGPEAIQITDKIFSGSVPSFSSHTAHLGTVSYNGQQIDQTLLLIMRAPRSFTGEDV
VELQCHGGYFSCSQILAALIAEGARPALPGEFSQRAFLNGKIDLIQAEAIQNIIAADNLDAFHIAQNHFQGHFSKKVQQI
SSLIIESLAFIEVLADFPEEEQPDMQEPMNRLHEAILIIDDLIASFDQGQRLAQGTSIVLAGHPNAGKSSLLNALTNKNR
AIVTDIPGTTRDILEENWMLQGKRIRLIDSAGQRETHNPIEQEGIERAIAAMEASEAILWVMDASQPPPPLPEILMRKPS
LLLWNKSDLATPPHLETTLPQLAVSAKTGAGMIELKQFIQQWMQKQQLGKNGKVFLISSRHHTILQHMRTYLLSAQEGLQ
SQSPPEFIALELRQALQTTGNLSGSEINETILGEIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=32.1888412017167, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI193082993, Length=450, Percent_Identity=31.5555555555556, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI193082988, Length=493, Percent_Identity=29.8174442190669, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI2367268, Length=458, Percent_Identity=37.9912663755458, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI87082120, Length=160, Percent_Identity=29.375, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=28.6334056399132, Blast_Score=163, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=32.5865580448065, Blast_Score=206, Evalue=8e-54,
Organism=Drosophila melanogaster, GI45550813, Length=476, Percent_Identity=28.781512605042, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CHLAB (Q5L4W0)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220282.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q5L4W0
- SMR:   Q5L4W0
- GeneID:   3337498
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB897
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q5L4W0
- ProtClustDB:   PRK05291
- BioCyc:   CABO218497:CAB897-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48575; Mature: 48575

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 82-82 BINDING 121-121 BINDING 443-443

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNDTIAAIATPPGEGSIAIVRISGPEAIQITDKIFSGSVPSFSSHTAHLGTVSYNGQQ
CCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEHHHHCCCCCCCCCCCEEEEEEECCCHH
IDQTLLLIMRAPRSFTGEDVVELQCHGGYFSCSQILAALIAEGARPALPGEFSQRAFLNG
HHHHHHHHHHCCCCCCCCCEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
KIDLIQAEAIQNIIAADNLDAFHIAQNHFQGHFSKKVQQISSLIIESLAFIEVLADFPEE
CEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQPDMQEPMNRLHEAILIIDDLIASFDQGQRLAQGTSIVLAGHPNAGKSSLLNALTNKNR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHCCCC
AIVTDIPGTTRDILEENWMLQGKRIRLIDSAGQRETHNPIEQEGIERAIAAMEASEAILW
EEEECCCCCHHHHHHHCCEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEE
VMDASQPPPPLPEILMRKPSLLLWNKSDLATPPHLETTLPQLAVSAKTGAGMIELKQFIQ
EEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
QWMQKQQLGKNGKVFLISSRHHTILQHMRTYLLSAQEGLQSQSPPEFIALELRQALQTTG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
NLSGSEINETILGEIFSRFCIGK
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKNDTIAAIATPPGEGSIAIVRISGPEAIQITDKIFSGSVPSFSSHTAHLGTVSYNGQQ
CCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEHHHHCCCCCCCCCCCEEEEEEECCCHH
IDQTLLLIMRAPRSFTGEDVVELQCHGGYFSCSQILAALIAEGARPALPGEFSQRAFLNG
HHHHHHHHHHCCCCCCCCCEEEEEECCCEECHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
KIDLIQAEAIQNIIAADNLDAFHIAQNHFQGHFSKKVQQISSLIIESLAFIEVLADFPEE
CEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EQPDMQEPMNRLHEAILIIDDLIASFDQGQRLAQGTSIVLAGHPNAGKSSLLNALTNKNR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCCHHHHHHHHHCCCC
AIVTDIPGTTRDILEENWMLQGKRIRLIDSAGQRETHNPIEQEGIERAIAAMEASEAILW
EEEECCCCCHHHHHHHCCEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEE
VMDASQPPPPLPEILMRKPSLLLWNKSDLATPPHLETTLPQLAVSAKTGAGMIELKQFIQ
EEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
QWMQKQQLGKNGKVFLISSRHHTILQHMRTYLLSAQEGLQSQSPPEFIALELRQALQTTG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
NLSGSEINETILGEIFSRFCIGK
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA