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The map label for this gene is braB [H]

Identifier: 62185499

GI number: 62185499

Start: 1050371

End: 1051600

Strand: Direct

Name: braB [H]

Synonym: CAB899

Alternate gene names: 62185499

Gene position: 1050371-1051600 (Clockwise)

Preceding gene: 62185498

Following gene: 62185500

Centisome position: 91.79

GC content: 38.54

Gene sequence:

>1230_bases
ATGAATAAAAACGCTTCTCATAGGGCTAGTTCTAAAAAAGAGCTCTCTGTTTGGTCGATAGGAGGCTCTATTTTCGCCAT
GTTCTTTGGAGCGGGCAATATAGTATTTCCACTAGCCTTAGGTTATCATTACCATGCCCACCCTTGGTTTGCTTGTTTCG
GCATGATCCTTAGCGCAGTCTGCGTTCCCCTACTGGGCTTATTCAGTATGTTATTATATTCCGGAGATTATAAAGCTTTT
TTTTCTTCCATTGGAAAAATGCCCGGAATGTTTTTCATCGTAGCCATTCTATGTTTGATAGGTCCTTTTGGAGGGATACC
TAGAGCGATCGCCGTTTCGCATGCTACTCTAGTGTCGCTATCCGATTCAAAAACAACATGGATTCCTCATCTTCCTGTTT
TCAGTTTGATTTGTTGTGTTTTGATTTATCTATTTGCTTGTAAATTCAGTAAACTTATCCAATGGTTAGGGTCTGTATTT
TTCCCCGTAATGTTAGTTACTCTAATTTGGATAATCTTTAAAGGATTGGTCATTCCTGCAAATCCTAGCTTTATGACATC
AGTAAGTTCCAGAGAAGCTTGGTCAGCAGGAATCATCGAAGGCTTCAATACTATGGACCTTCTTGCAGCTTTCTTCTTTT
GCTCTATTGTTGTAATTTCTATAAGACAACTGTTAGAAAATAGAGAGGCGAGTGATGAAACACCTTTAAACTTTCAAAAA
ATCAACAAAAAAGACAAACGCACTTTAGGTTTAGCTTTTGCTTTGGCTGCAGCACTTCTTGGATTAATTTATCTAGGATT
TGCGTTGTGTGCTTCTCGGCACGCAGGTTTGCTAGCCCATGTAAGTAAAGGACAAATTCTAGGAAGAATTTCTGCTATTG
CTCTCGGACCAAATAGTTTATTAACCGGTGTATGTGTGTTTGTAGCTTGCTTAACGACAGAGATAGCTTTAGTTGGGATC
TTTGCTGACTTTTTAGCCCGCATCATCTCATCAAAAAGAATGACGTACTCTAATGCAGTAATTTTCACTCTGATACCCTC
ATACCTAATTTCTATTTTAAACTTCGAAAATATCAGTCTCCTTCTATTACCTTTACTACAGCTAAGTTATCCTGCATTAA
TCGCTTTGACTTGCGGAAGTATTGCTTATAAGTTGTGGAATTTCCGACACGTCCAAGCTTTGTTTTATTTAACCCTCTCT
CTTACAATCGTTTTACGGTTGGTGAGTTGA

Upstream 100 bases:

>100_bases
CTCTGTATATGTCATGGATGAAATTTACTTTGCTTTCTAACGTAGAAATCTTTTAGACTGGGCACCTACTTACTACCTCG
ATATCGCAATTTTTAATATA

Downstream 100 bases:

>100_bases
GTTAACGCTTGTATTTGCTAACTAGCATAAAGTTATGGTTTTAAACGCTTTAGCTGGCTTTCGTCAAACAGCGATGGATT
ACCTCGCAACGAATCGCCAC

Product: putative branched-chain amino acid transport protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MNKNASHRASSKKELSVWSIGGSIFAMFFGAGNIVFPLALGYHYHAHPWFACFGMILSAVCVPLLGLFSMLLYSGDYKAF
FSSIGKMPGMFFIVAILCLIGPFGGIPRAIAVSHATLVSLSDSKTTWIPHLPVFSLICCVLIYLFACKFSKLIQWLGSVF
FPVMLVTLIWIIFKGLVIPANPSFMTSVSSREAWSAGIIEGFNTMDLLAAFFFCSIVVISIRQLLENREASDETPLNFQK
INKKDKRTLGLAFALAAALLGLIYLGFALCASRHAGLLAHVSKGQILGRISAIALGPNSLLTGVCVFVACLTTEIALVGI
FADFLARIISSKRMTYSNAVIFTLIPSYLISILNFENISLLLLPLLQLSYPALIALTCGSIAYKLWNFRHVQALFYLTLS
LTIVLRLVS

Sequences:

>Translated_409_residues
MNKNASHRASSKKELSVWSIGGSIFAMFFGAGNIVFPLALGYHYHAHPWFACFGMILSAVCVPLLGLFSMLLYSGDYKAF
FSSIGKMPGMFFIVAILCLIGPFGGIPRAIAVSHATLVSLSDSKTTWIPHLPVFSLICCVLIYLFACKFSKLIQWLGSVF
FPVMLVTLIWIIFKGLVIPANPSFMTSVSSREAWSAGIIEGFNTMDLLAAFFFCSIVVISIRQLLENREASDETPLNFQK
INKKDKRTLGLAFALAAALLGLIYLGFALCASRHAGLLAHVSKGQILGRISAIALGPNSLLTGVCVFVACLTTEIALVGI
FADFLARIISSKRMTYSNAVIFTLIPSYLISILNFENISLLLLPLLQLSYPALIALTCGSIAYKLWNFRHVQALFYLTLS
LTIVLRLVS
>Mature_409_residues
MNKNASHRASSKKELSVWSIGGSIFAMFFGAGNIVFPLALGYHYHAHPWFACFGMILSAVCVPLLGLFSMLLYSGDYKAF
FSSIGKMPGMFFIVAILCLIGPFGGIPRAIAVSHATLVSLSDSKTTWIPHLPVFSLICCVLIYLFACKFSKLIQWLGSVF
FPVMLVTLIWIIFKGLVIPANPSFMTSVSSREAWSAGIIEGFNTMDLLAAFFFCSIVVISIRQLLENREASDETPLNFQK
INKKDKRTLGLAFALAAALLGLIYLGFALCASRHAGLLAHVSKGQILGRISAIALGPNSLLTGVCVFVACLTTEIALVGI
FADFLARIISSKRMTYSNAVIFTLIPSYLISILNFENISLLLLPLLQLSYPALIALTCGSIAYKLWNFRHVQALFYLTLS
LTIVLRLVS

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=387, Percent_Identity=26.3565891472868, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 44727; Mature: 44727

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNASHRASSKKELSVWSIGGSIFAMFFGAGNIVFPLALGYHYHAHPWFACFGMILSAV
CCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
CVPLLGLFSMLLYSGDYKAFFSSIGKMPGMFFIVAILCLIGPFGGIPRAIAVSHATLVSL
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEE
SDSKTTWIPHLPVFSLICCVLIYLFACKFSKLIQWLGSVFFPVMLVTLIWIIFKGLVIPA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCC
NPSFMTSVSSREAWSAGIIEGFNTMDLLAAFFFCSIVVISIRQLLENREASDETPLNFQK
CCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
INKKDKRTLGLAFALAAALLGLIYLGFALCASRHAGLLAHVSKGQILGRISAIALGPNSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHEEECCHHH
LTGVCVFVACLTTEIALVGIFADFLARIISSKRMTYSNAVIFTLIPSYLISILNFENISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCCCHHH
LLLPLLQLSYPALIALTCGSIAYKLWNFRHVQALFYLTLSLTIVLRLVS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKNASHRASSKKELSVWSIGGSIFAMFFGAGNIVFPLALGYHYHAHPWFACFGMILSAV
CCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
CVPLLGLFSMLLYSGDYKAFFSSIGKMPGMFFIVAILCLIGPFGGIPRAIAVSHATLVSL
HHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHEEEE
SDSKTTWIPHLPVFSLICCVLIYLFACKFSKLIQWLGSVFFPVMLVTLIWIIFKGLVIPA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCC
NPSFMTSVSSREAWSAGIIEGFNTMDLLAAFFFCSIVVISIRQLLENREASDETPLNFQK
CCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
INKKDKRTLGLAFALAAALLGLIYLGFALCASRHAGLLAHVSKGQILGRISAIALGPNSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHEEECCHHH
LTGVCVFVACLTTEIALVGIFADFLARIISSKRMTYSNAVIFTLIPSYLISILNFENISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHCCCCHHH
LLLPLLQLSYPALIALTCGSIAYKLWNFRHVQALFYLTLSLTIVLRLVS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]