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The map label for this gene is cca [H]

Identifier: 62185492

GI number: 62185492

Start: 1039255

End: 1040487

Strand: Direct

Name: cca [H]

Synonym: CAB890

Alternate gene names: 62185492

Gene position: 1039255-1040487 (Clockwise)

Preceding gene: 62185491

Following gene: 62185493

Centisome position: 90.81

GC content: 41.36

Gene sequence:

>1233_bases
ATGACAACAATCGCCCTAGAAGCTGCAAAAAAGATTCTCCTGAAATTGCGTAATGCCGGCTACCAAGCCTATTTTGTTGG
TGGTTGTGTTCGCGATATGCTCATGGGCAAGCCCATAGAAGAAATTGACATAGCTACTAGCGCTCCTCCCGTTATTGTTT
CTACTATCTTTCCTGACACACTAGCCATAGGAGCCGCTTTCGGTATTATCGTCGTTAAAGAAAACAACCGCTTATTCGAA
GTAGCTACATTTCGTAGTGATGAAAATTATACAGACGGACGGCATCCTGAACGTGTGATATTTTCATCAATGAAAGAAGA
TGCTTTACGCCGTGATTTTACAATAAACGGGATGTATTACGATCCCTTTGAAGAAAAGCTCTATGATCTTGTTGAAGGAA
GAAGAGATCTCGAAAAGCGTGTCATACGTGCTATTGGTCATCCTAAATTACGTTTCTCCGAAGATAAACTCCGTATATTA
CGTGCTATACGTTTTAGTTCCTCTTTAGGATTTACTTTAGATCCTGCTACCGAACGTGCCATTATTAAGGAAGCCCCTTC
GTTGGTATGTTCCGTATCTCCAGAAAGAATCTGGCAGGAACTAAAGAAAATGCTGAAAAAAAATCCTTACGAAGCTCTAT
CCTTGCTGATACAGCTGAAAATCATGACGATAATTTTCCCCGAGCTTCGGGATATTCCTCCAAGCTTACTACGCACAAGT
AGCGAATTTGCTAAAAGACTGAATCCGCTAGATTTCCCTGAAATTTGCTTTTTACTTCCTCTATTTCAAGGTGTGAGCGA
AGAAGCGGCTTGTGTAGCTTTTACTCGTTTACGAATCTCAAACAAAGATTTGAAACTCATACAACTGTGGTATCAAGCAC
TTCCTCAGTTCCAGAATGTCAGTAATAATCGTGTGTTCTGGGCGCACTTTCTAGCCTCCCCGACAGCACACCTATTTCTA
TCTCTATTTTCAGCAACCCAAAAAGATCCTTCCCAACAACAACATTTCATCGCCCGCGTTCAAGAACTAGAAGAACGCTT
AGAACAATTCATTGTGAGAATCAAAACTGCGTCGCCCTTAGTTTCTGCTCCTGATCTCATCGCTAGGGGGATTGCACCAG
GCAGACTACTCGGAGATCTATTAAAAGAAGCCGAGATACTTTCTATAGAAAACGAGTGTCATGATAAAGAAAAAATTTTA
ACCCTGCTAAAGGAAAAGGGGTTCTGGAAGTAA

Upstream 100 bases:

>100_bases
TTCAAGAAGACACGATCTTAGAGAATAAAGCTCCGGTAATTATCAGAAGAGCTCCAGAAGCTATAGCTTAAATTTCTCTT
CTTCGTTATTTAGGGATGTA

Downstream 100 bases:

>100_bases
GATAAATCACAAAATAAACTTTTCCCTTAAACACGTCATCAATTTTATTTCTTTAGATTTAATTTTTTAGCTCTTATTAT
TTTTCCTATAATTATTCAAA

Product: putative poly A polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTTIALEAAKKILLKLRNAGYQAYFVGGCVRDMLMGKPIEEIDIATSAPPVIVSTIFPDTLAIGAAFGIIVVKENNRLFE
VATFRSDENYTDGRHPERVIFSSMKEDALRRDFTINGMYYDPFEEKLYDLVEGRRDLEKRVIRAIGHPKLRFSEDKLRIL
RAIRFSSSLGFTLDPATERAIIKEAPSLVCSVSPERIWQELKKMLKKNPYEALSLLIQLKIMTIIFPELRDIPPSLLRTS
SEFAKRLNPLDFPEICFLLPLFQGVSEEAACVAFTRLRISNKDLKLIQLWYQALPQFQNVSNNRVFWAHFLASPTAHLFL
SLFSATQKDPSQQQHFIARVQELEERLEQFIVRIKTASPLVSAPDLIARGIAPGRLLGDLLKEAEILSIENECHDKEKIL
TLLKEKGFWK

Sequences:

>Translated_410_residues
MTTIALEAAKKILLKLRNAGYQAYFVGGCVRDMLMGKPIEEIDIATSAPPVIVSTIFPDTLAIGAAFGIIVVKENNRLFE
VATFRSDENYTDGRHPERVIFSSMKEDALRRDFTINGMYYDPFEEKLYDLVEGRRDLEKRVIRAIGHPKLRFSEDKLRIL
RAIRFSSSLGFTLDPATERAIIKEAPSLVCSVSPERIWQELKKMLKKNPYEALSLLIQLKIMTIIFPELRDIPPSLLRTS
SEFAKRLNPLDFPEICFLLPLFQGVSEEAACVAFTRLRISNKDLKLIQLWYQALPQFQNVSNNRVFWAHFLASPTAHLFL
SLFSATQKDPSQQQHFIARVQELEERLEQFIVRIKTASPLVSAPDLIARGIAPGRLLGDLLKEAEILSIENECHDKEKIL
TLLKEKGFWK
>Mature_409_residues
TTIALEAAKKILLKLRNAGYQAYFVGGCVRDMLMGKPIEEIDIATSAPPVIVSTIFPDTLAIGAAFGIIVVKENNRLFEV
ATFRSDENYTDGRHPERVIFSSMKEDALRRDFTINGMYYDPFEEKLYDLVEGRRDLEKRVIRAIGHPKLRFSEDKLRILR
AIRFSSSLGFTLDPATERAIIKEAPSLVCSVSPERIWQELKKMLKKNPYEALSLLIQLKIMTIIFPELRDIPPSLLRTSS
EFAKRLNPLDFPEICFLLPLFQGVSEEAACVAFTRLRISNKDLKLIQLWYQALPQFQNVSNNRVFWAHFLASPTAHLFLS
LFSATQKDPSQQQHFIARVQELEERLEQFIVRIKTASPLVSAPDLIARGIAPGRLLGDLLKEAEILSIENECHDKEKILT
LLKEKGFWK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=38.3495145631068, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI87081691, Length=235, Percent_Identity=37.4468085106383, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789436, Length=216, Percent_Identity=29.1666666666667, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=205, Percent_Identity=33.6585365853659, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321016, Length=233, Percent_Identity=29.1845493562232, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=204, Percent_Identity=39.2156862745098, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357337, Length=204, Percent_Identity=39.2156862745098, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46809; Mature: 46678

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIALEAAKKILLKLRNAGYQAYFVGGCVRDMLMGKPIEEIDIATSAPPVIVSTIFPDT
CCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCHHHHCCCCCCCCEEEEHHCCHH
LAIGAAFGIIVVKENNRLFEVATFRSDENYTDGRHPERVIFSSMKEDALRRDFTINGMYY
HHHHHHEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCEEE
DPFEEKLYDLVEGRRDLEKRVIRAIGHPKLRFSEDKLRILRAIRFSSSLGFTLDPATERA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCEECCHHHHH
IIKEAPSLVCSVSPERIWQELKKMLKKNPYEALSLLIQLKIMTIIFPELRDIPPSLLRTS
HHHHCCHHEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCH
SEFAKRLNPLDFPEICFLLPLFQGVSEEAACVAFTRLRISNKDLKLIQLWYQALPQFQNV
HHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
SNNRVFWAHFLASPTAHLFLSLFSATQKDPSQQQHFIARVQELEERLEQFIVRIKTASPL
CCCEEEEEHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAPDLIARGIAPGRLLGDLLKEAEILSIENECHDKEKILTLLKEKGFWK
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTIALEAAKKILLKLRNAGYQAYFVGGCVRDMLMGKPIEEIDIATSAPPVIVSTIFPDT
CCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCHHHHCCCCCCCCEEEEHHCCHH
LAIGAAFGIIVVKENNRLFEVATFRSDENYTDGRHPERVIFSSMKEDALRRDFTINGMYY
HHHHHHEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCEEE
DPFEEKLYDLVEGRRDLEKRVIRAIGHPKLRFSEDKLRILRAIRFSSSLGFTLDPATERA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCEECCHHHHH
IIKEAPSLVCSVSPERIWQELKKMLKKNPYEALSLLIQLKIMTIIFPELRDIPPSLLRTS
HHHHCCHHEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCH
SEFAKRLNPLDFPEICFLLPLFQGVSEEAACVAFTRLRISNKDLKLIQLWYQALPQFQNV
HHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
SNNRVFWAHFLASPTAHLFLSLFSATQKDPSQQQHFIARVQELEERLEQFIVRIKTASPL
CCCEEEEEHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAPDLIARGIAPGRLLGDLLKEAEILSIENECHDKEKILTLLKEKGFWK
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA