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The map label for this gene is purN [H]

Identifier: 59712536

GI number: 59712536

Start: 2158304

End: 2158945

Strand: Direct

Name: purN [H]

Synonym: VF_1929

Alternate gene names: 59712536

Gene position: 2158304-2158945 (Clockwise)

Preceding gene: 59712535

Following gene: 172087736

Centisome position: 74.49

GC content: 39.25

Gene sequence:

>642_bases
ATGATGAAGAATATTGTTGTTTTAGTTTCAGGTAATGGCAGCAACTTACAAGCCTTTATTGATGCTTGTGGCAATAAAAT
CCCTAATGCACGTATCGCTGCTGTTATTTCTAACAAAAGTGATGCTTACGGATTACAGAGAGCCATTGATGCTGATATTA
ATGTGCATTCTTTAAATGCCAAAGCATATGACAGTCGTGAGCTATATGATGACGCTCTAGCTACTCTAATCGATCTTCAC
AAACCCGATATCATTATCCTTGCTGGATTCATGCGAATTTTAAGTGAAGCATTTGTCACTCGATACCAAGGAAAAATGCT
CAATATCCACCCTTCTCTTTTGCCAAAATACACGGGATTACATACTCATCAACGTGCTATTGATGCTGGAGATAAAGAGC
ATGGAACAAGTGTGCATTTCGTCACTCCAGAATTAGATGGTGGACCTGTTATCCTTCAAGCTAAAGTTCCTATCTTTGAA
AATGATAATGCAGAAGATGTAGCATCTCGTGTACAAGCCCAAGAACATGTCATTTACCCTATGGTAGCGAATTGGTTGGT
AGAAGAAAGATTAACCATGGTCGATGGGAAAGCGATTCTTGATGGTACCGAATTAGGTCTATCCGGTTTAGAGACAGAAT
AG

Upstream 100 bases:

>100_bases
TTCATAAAGAAAAAATAAAAGGTGATCATTTAATCGATCACCTTTTTTATAGCTATTATTTATGTTCGAATAGTTCTCAT
TACAAATTAAAAAGATAGGT

Downstream 100 bases:

>100_bases
ATACAAAAAAGCCGCTTAAATAACTAAGCGGCTTTTTAAGTTCTATGTACTTTTAATGCTAAAGAGCACTATTTACTGGA
CACTCAGATGGAGGTGGTGC

Product: phosphoribosylglycinamide formyltransferase 1

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MMKNIVVLVSGNGSNLQAFIDACGNKIPNARIAAVISNKSDAYGLQRAIDADINVHSLNAKAYDSRELYDDALATLIDLH
KPDIIILAGFMRILSEAFVTRYQGKMLNIHPSLLPKYTGLHTHQRAIDAGDKEHGTSVHFVTPELDGGPVILQAKVPIFE
NDNAEDVASRVQAQEHVIYPMVANWLVEERLTMVDGKAILDGTELGLSGLETE

Sequences:

>Translated_213_residues
MMKNIVVLVSGNGSNLQAFIDACGNKIPNARIAAVISNKSDAYGLQRAIDADINVHSLNAKAYDSRELYDDALATLIDLH
KPDIIILAGFMRILSEAFVTRYQGKMLNIHPSLLPKYTGLHTHQRAIDAGDKEHGTSVHFVTPELDGGPVILQAKVPIFE
NDNAEDVASRVQAQEHVIYPMVANWLVEERLTMVDGKAILDGTELGLSGLETE
>Mature_213_residues
MMKNIVVLVSGNGSNLQAFIDACGNKIPNARIAAVISNKSDAYGLQRAIDADINVHSLNAKAYDSRELYDDALATLIDLH
KPDIIILAGFMRILSEAFVTRYQGKMLNIHPSLLPKYTGLHTHQRAIDAGDKEHGTSVHFVTPELDGGPVILQAKVPIFE
NDNAEDVASRVQAQEHVIYPMVANWLVEERLTMVDGKAILDGTELGLSGLETE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=44.0677966101695, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=44.0677966101695, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=44.0677966101695, Blast_Score=155, Evalue=3e-38,
Organism=Escherichia coli, GI1788846, Length=206, Percent_Identity=61.1650485436893, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1787483, Length=165, Percent_Identity=31.5151515151515, Blast_Score=86, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=176, Percent_Identity=34.0909090909091, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=36.1809045226131, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=45.4545454545455, Blast_Score=167, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23272; Mature: 23272

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKNIVVLVSGNGSNLQAFIDACGNKIPNARIAAVISNKSDAYGLQRAIDADINVHSLNA
CCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCC
KAYDSRELYDDALATLIDLHKPDIIILAGFMRILSEAFVTRYQGKMLNIHPSLLPKYTGL
CCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCC
HTHQRAIDAGDKEHGTSVHFVTPELDGGPVILQAKVPIFENDNAEDVASRVQAQEHVIYP
CHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHCCHHH
MVANWLVEERLTMVDGKAILDGTELGLSGLETE
HHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCC
>Mature Secondary Structure
MMKNIVVLVSGNGSNLQAFIDACGNKIPNARIAAVISNKSDAYGLQRAIDADINVHSLNA
CCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCC
KAYDSRELYDDALATLIDLHKPDIIILAGFMRILSEAFVTRYQGKMLNIHPSLLPKYTGL
CCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEECHHHCCHHCCC
HTHQRAIDAGDKEHGTSVHFVTPELDGGPVILQAKVPIFENDNAEDVASRVQAQEHVIYP
CHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCEECCCCHHHHHHHHHHHHCCHHH
MVANWLVEERLTMVDGKAILDGTELGLSGLETE
HHHHHHHHHHHHHCCCCEEECCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]