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The map label for this gene is rotA [H]

Identifier: 59711328

GI number: 59711328

Start: 788641

End: 789201

Strand: Reverse

Name: rotA [H]

Synonym: VF_0721

Alternate gene names: 59711328

Gene position: 789201-788641 (Counterclockwise)

Preceding gene: 59711329

Following gene: 59711327

Centisome position: 27.24

GC content: 37.61

Gene sequence:

>561_bases
ATGATTAAAATGATTTCAAAAGCCGCATTAATTATTGCTGCTCTTAGCACAAGTCAAATGGCTCTAGCAGAATCGGTAAA
ATTTGAAACCACATTGGGTAACTTTACTGTTGAACTAAATTCAGAGAAAGCTCCTATTTCTGTGGCAAACTTCATCCGTT
ATGTTGAAGATGGCAGTTATGTTGGAACTCAATTTCACCGAGTAATCCCAGGCTTCATGGCTCAAGGTGGTGGTTTTGAT
AAAAATATGATAAAAAAGCCAAGCTATGGGCCTATCAAAAATGAAGGCTATAACGGTTTAGGTAATGACACTGCTACAAT
TGCAATGGCTCGAACTAACAACCCAGATTCTGCCACTCGCCAATTCTACATCAACCTAAATGACAATGATTTCTTAAACG
CAAGTCAGCGTCCACCGGGTTATGCAGTGTTTGGTAAAGTAACACAAGGTTTTGATGTGATTCAAAAAATGGCAACTAAG
AAAACCAAAGCTCTACCAAATGGTATGCGCGATATTCCAGTTGAGCCAATTATCATAACTAAAGCAACATTAATTAAATA
A

Upstream 100 bases:

>100_bases
ATCAAGACATTGAACAAGTTCTTAATGACTTATTAAATGCAGTATTAGATAAAATCGCTGTTGATGAAGAATTACAAACA
TACATTAAAGGTAACTTTTA

Downstream 100 bases:

>100_bases
TTTAGTTAATATAGCACTACTCTAAAGCAGCTTTCGGGCTGCTTTTTCTATCTATTTCAATAAGAACAAATAACAAGGAG
CGTTTCATGTCTCATTCCAT

Product: peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MIKMISKAALIIAALSTSQMALAESVKFETTLGNFTVELNSEKAPISVANFIRYVEDGSYVGTQFHRVIPGFMAQGGGFD
KNMIKKPSYGPIKNEGYNGLGNDTATIAMARTNNPDSATRQFYINLNDNDFLNASQRPPGYAVFGKVTQGFDVIQKMATK
KTKALPNGMRDIPVEPIIITKATLIK

Sequences:

>Translated_186_residues
MIKMISKAALIIAALSTSQMALAESVKFETTLGNFTVELNSEKAPISVANFIRYVEDGSYVGTQFHRVIPGFMAQGGGFD
KNMIKKPSYGPIKNEGYNGLGNDTATIAMARTNNPDSATRQFYINLNDNDFLNASQRPPGYAVFGKVTQGFDVIQKMATK
KTKALPNGMRDIPVEPIIITKATLIK
>Mature_186_residues
MIKMISKAALIIAALSTSQMALAESVKFETTLGNFTVELNSEKAPISVANFIRYVEDGSYVGTQFHRVIPGFMAQGGGFD
KNMIKKPSYGPIKNEGYNGLGNDTATIAMARTNNPDSATRQFYINLNDNDFLNASQRPPGYAVFGKVTQGFDVIQKMATK
KTKALPNGMRDIPVEPIIITKATLIK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI64276486, Length=130, Percent_Identity=36.9230769230769, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI24308049, Length=182, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1789763, Length=180, Percent_Identity=48.3333333333333, Blast_Score=160, Evalue=4e-41,
Organism=Escherichia coli, GI1786736, Length=163, Percent_Identity=47.239263803681, Blast_Score=137, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71980594, Length=147, Percent_Identity=30.6122448979592, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71980590, Length=147, Percent_Identity=30.6122448979592, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17552780, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17986117, Length=163, Percent_Identity=31.2883435582822, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136360, Length=190, Percent_Identity=28.4210526315789, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20274; Mature: 20274

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMISKAALIIAALSTSQMALAESVKFETTLGNFTVELNSEKAPISVANFIRYVEDGSY
CCCHHHHHEEEEHHHCCHHHHHHHHCEEEEEECEEEEEECCCCCCCHHHHHHHHHCCCCE
VGTQFHRVIPGFMAQGGGFDKNMIKKPSYGPIKNEGYNGLGNDTATIAMARTNNPDSATR
EHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCE
QFYINLNDNDFLNASQRPPGYAVFGKVTQGFDVIQKMATKKTKALPNGMRDIPVEPIIIT
EEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
KATLIK
CEECCC
>Mature Secondary Structure
MIKMISKAALIIAALSTSQMALAESVKFETTLGNFTVELNSEKAPISVANFIRYVEDGSY
CCCHHHHHEEEEHHHCCHHHHHHHHCEEEEEECEEEEEECCCCCCCHHHHHHHHHCCCCE
VGTQFHRVIPGFMAQGGGFDKNMIKKPSYGPIKNEGYNGLGNDTATIAMARTNNPDSATR
EHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCE
QFYINLNDNDFLNASQRPPGYAVFGKVTQGFDVIQKMATKKTKALPNGMRDIPVEPIIIT
EEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
KATLIK
CEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]