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The map label for this gene is ispA [H]

Identifier: 59711319

GI number: 59711319

Start: 779768

End: 780673

Strand: Reverse

Name: ispA [H]

Synonym: VF_0712

Alternate gene names: 59711319

Gene position: 780673-779768 (Counterclockwise)

Preceding gene: 59711320

Following gene: 59711318

Centisome position: 26.94

GC content: 38.41

Gene sequence:

>906_bases
ATGGACAAGATGACTGATTCACTAAAACAACAACTTACATTTTTTCAAAATAGAAACATTGAACAATTAAATCAGTGGCT
TGAAAAAATCGAAATTCAGCAACAACCACTAACTGAAGCAATGAAATATTCATTGTTGTTAGGTGGCAAACGAGCTCGAC
CATATTTAGTTTATATCACAGCACAAATGCTTGGCATTAAAGCTGAATTTGCTGATAGTGCAGCCGCAGCTATTGAATGT
GTCCACTCTTATTCATTAATCCATGATGATTTACCAGCAATGGATAATGATGATCTTCGTCGCGGTCATGCTACTTGCCA
TATAAAATATGATGAAGCAACAGCGATTCTTGCTGGTGATGCGCTCCAAACACTTGCTTTTGAAATATTAGCGAATGGTC
AATTAAGTGACGATGCAGAAACATCACGCATTGATATGATCCGCGTATTATCAAAAGCATCAGGTGCATCAGGTATGTGC
ATGGGACAATCATTAGATTTACAAGCTGAAGGTAAAAAAGTTGACCTTGCCTCTCTTGAGCGCATTCATGCTAATAAAAC
GGGTGCTCTTATTAAGTGTGCGGTTCAACTCGGTGCTCTTTGTGCTGGTGATAAAGGCAAAGCCATACTTCCACAATTAG
AAATCTATGCAGATGCTATTGGTCTCGCCTTTCAAGTACAAGATGATATCTTAGATATCATTAGTGATACTGAAACTCTC
GGAAAACCACAAGGTTCTGATCTAGAATTAGATAAAAGCACCTATCCTGCTTTACTTGGCCTTGAAGGCGCTAAACATAA
AGCAGAACAACTGTATCAAACAGCAATTGAAGCATTAAACGACATCCCTTATAACACGGATGCAATGCAAGCGTTCGCTC
AATACGTTATTGAGCGTAAAAACTAA

Upstream 100 bases:

>100_bases
ATTTCTCTAGCACGAGCTGGACAAGAAAAACTAACTCAAGCAGAACAACGAGTAGAAATTCTTCTTCAAGCTGACGATAA
CGCAGAACTAACACCTTTTG

Downstream 100 bases:

>100_bases
AACAGATAAGCGCTAACTACTATGTCATTAGATATTTCAAAATACCCAATTCTGGCTTTGGCAAATACACCAGATGAATT
ACGTTCTTTACCAAAAGAGA

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDKMTDSLKQQLTFFQNRNIEQLNQWLEKIEIQQQPLTEAMKYSLLLGGKRARPYLVYITAQMLGIKAEFADSAAAAIEC
VHSYSLIHDDLPAMDNDDLRRGHATCHIKYDEATAILAGDALQTLAFEILANGQLSDDAETSRIDMIRVLSKASGASGMC
MGQSLDLQAEGKKVDLASLERIHANKTGALIKCAVQLGALCAGDKGKAILPQLEIYADAIGLAFQVQDDILDIISDTETL
GKPQGSDLELDKSTYPALLGLEGAKHKAEQLYQTAIEALNDIPYNTDAMQAFAQYVIERKN

Sequences:

>Translated_301_residues
MDKMTDSLKQQLTFFQNRNIEQLNQWLEKIEIQQQPLTEAMKYSLLLGGKRARPYLVYITAQMLGIKAEFADSAAAAIEC
VHSYSLIHDDLPAMDNDDLRRGHATCHIKYDEATAILAGDALQTLAFEILANGQLSDDAETSRIDMIRVLSKASGASGMC
MGQSLDLQAEGKKVDLASLERIHANKTGALIKCAVQLGALCAGDKGKAILPQLEIYADAIGLAFQVQDDILDIISDTETL
GKPQGSDLELDKSTYPALLGLEGAKHKAEQLYQTAIEALNDIPYNTDAMQAFAQYVIERKN
>Mature_301_residues
MDKMTDSLKQQLTFFQNRNIEQLNQWLEKIEIQQQPLTEAMKYSLLLGGKRARPYLVYITAQMLGIKAEFADSAAAAIEC
VHSYSLIHDDLPAMDNDDLRRGHATCHIKYDEATAILAGDALQTLAFEILANGQLSDDAETSRIDMIRVLSKASGASGMC
MGQSLDLQAEGKKVDLASLERIHANKTGALIKCAVQLGALCAGDKGKAILPQLEIYADAIGLAFQVQDDILDIISDTETL
GKPQGSDLELDKSTYPALLGLEGAKHKAEQLYQTAIEALNDIPYNTDAMQAFAQYVIERKN

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786623, Length=297, Percent_Identity=55.5555555555556, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789578, Length=269, Percent_Identity=29.7397769516729, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17505681, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24651612, Length=186, Percent_Identity=33.8709677419355, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32981; Mature: 32981

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKMTDSLKQQLTFFQNRNIEQLNQWLEKIEIQQQPLTEAMKYSLLLGGKRARPYLVYIT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEE
AQMLGIKAEFADSAAAAIECVHSYSLIHDDLPAMDNDDLRRGHATCHIKYDEATAILAGD
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCCEEEEEHH
ALQTLAFEILANGQLSDDAETSRIDMIRVLSKASGASGMCMGQSLDLQAEGKKVDLASLE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEECHHHHH
RIHANKTGALIKCAVQLGALCAGDKGKAILPQLEIYADAIGLAFQVQDDILDIISDTETL
HHHCCCCCHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHEEEEHHHHHHHHHCHHHH
GKPQGSDLELDKSTYPALLGLEGAKHKAEQLYQTAIEALNDIPYNTDAMQAFAQYVIERK
CCCCCCCEEECCCCCCHHEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
N
C
>Mature Secondary Structure
MDKMTDSLKQQLTFFQNRNIEQLNQWLEKIEIQQQPLTEAMKYSLLLGGKRARPYLVYIT
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEE
AQMLGIKAEFADSAAAAIECVHSYSLIHDDLPAMDNDDLRRGHATCHIKYDEATAILAGD
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCCEEEEEHH
ALQTLAFEILANGQLSDDAETSRIDMIRVLSKASGASGMCMGQSLDLQAEGKKVDLASLE
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEECHHHHH
RIHANKTGALIKCAVQLGALCAGDKGKAILPQLEIYADAIGLAFQVQDDILDIISDTETL
HHHCCCCCHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHEEEEHHHHHHHHHCHHHH
GKPQGSDLELDKSTYPALLGLEGAKHKAEQLYQTAIEALNDIPYNTDAMQAFAQYVIERK
CCCCCCCEEECCCCCCHHEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2089044; 9278503 [H]