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The map label for this gene is raiA [H]

Identifier: 59711169

GI number: 59711169

Start: 610517

End: 610870

Strand: Reverse

Name: raiA [H]

Synonym: VF_0562

Alternate gene names: 59711169

Gene position: 610870-610517 (Counterclockwise)

Preceding gene: 59711170

Following gene: 59711168

Centisome position: 21.08

GC content: 42.66

Gene sequence:

>354_bases
ATGAAAGTAGAAATCACTGGCAACAACATCGACATCACTCCAGGCATCCGTGAACGTATTGAAGGGAAGTTTAAAAAACT
AGAAGCAAAAAGAAACCTAGACATTATTGGACGTCACGCTAGCGTTACAAAACGCGCAAATGGTAAACACAAGGTAGAAG
CGTACGCAACTATCGCAGGTGGCAAAGTCGTAGCCTCTGCAGAGCAAGAAGATCTATTTGGTGCAATTAGAGAGATGTAT
CAAAAACTAGAACGCCAACTAGACAAACTACAACATAAAGGAGAAGCACGTCGAGCGACTCATGCTCAAGCTCCTGGAGT
TGAAGAACCAGAAGTTGAACTTGAAGAGGAATAA

Upstream 100 bases:

>100_bases
CGCTACAACACATCAAAAAAGTGATCTTGATCACTTTATTTTTAACTCTGGATACGTAATCTGAGTACATGCCAAACGGG
GCAAGCGAGAGGAAAATCAT

Downstream 100 bases:

>100_bases
TATTCAACTCTAAGATTCTTAAGAACAGCGCTCATTTGAGCGCTGTTTTTTCTTGACGCAAACTAACCAATTCCTTATTG
TGAATTAACTCTTAATTTAC

Product: stationary phase translation inhibitor and ribosome stability factor RaiA (YfiA)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MKVEITGNNIDITPGIRERIEGKFKKLEAKRNLDIIGRHASVTKRANGKHKVEAYATIAGGKVVASAEQEDLFGAIREMY
QKLERQLDKLQHKGEARRATHAQAPGVEEPEVELEEE

Sequences:

>Translated_117_residues
MKVEITGNNIDITPGIRERIEGKFKKLEAKRNLDIIGRHASVTKRANGKHKVEAYATIAGGKVVASAEQEDLFGAIREMY
QKLERQLDKLQHKGEARRATHAQAPGVEEPEVELEEE
>Mature_117_residues
MKVEITGNNIDITPGIRERIEGKFKKLEAKRNLDIIGRHASVTKRANGKHKVEAYATIAGGKVVASAEQEDLFGAIREMY
QKLERQLDKLQHKGEARRATHAQAPGVEEPEVELEEE

Specific function: During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation initiation and increase translation accuracy. When normal growth conditions are restored,

COG id: COG1544

COG function: function code J; Ribosome-associated protein Y (PSrp-1)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma(54) modulation protein family [H]

Homologues:

Organism=Escherichia coli, GI1788949, Length=99, Percent_Identity=43.4343434343434, Blast_Score=79, Evalue=9e-17,
Organism=Escherichia coli, GI1789595, Length=89, Percent_Identity=40.4494382022472, Blast_Score=62, Evalue=7e-12,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003489 [H]

Pfam domain/function: PF02482 Ribosomal_S30AE [H]

EC number: NA

Molecular weight: Translated: 13116; Mature: 13116

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEITGNNIDITPGIRERIEGKFKKLEAKRNLDIIGRHASVTKRANGKHKVEAYATIAG
CEEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCEEEEEEEEECC
GKVVASAEQEDLFGAIREMYQKLERQLDKLQHKGEARRATHAQAPGVEEPEVELEEE
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVEITGNNIDITPGIRERIEGKFKKLEAKRNLDIIGRHASVTKRANGKHKVEAYATIAG
CEEEECCCCEEECCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCEEEEEEEEECC
GKVVASAEQEDLFGAIREMYQKLERQLDKLQHKGEARRATHAQAPGVEEPEVELEEE
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]