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The map label for this gene is pheA [H]

Identifier: 59711168

GI number: 59711168

Start: 609224

End: 610402

Strand: Reverse

Name: pheA [H]

Synonym: VF_0561

Alternate gene names: 59711168

Gene position: 610402-609224 (Counterclockwise)

Preceding gene: 59711169

Following gene: 59711167

Centisome position: 21.07

GC content: 36.39

Gene sequence:

>1179_bases
ATGACTGACACTCACTACTCCCTTGATGATATTCGTTTACGCCTTAATGAACTTGATAATGAATTATTAAAGCTCTTTTC
AGAACGTCGTAAACTCAGTCTTGATGTTGCTAAAAGTAAAGTTAAAACCGCGAAACCTGTTCGAGATCCTAAACGAGAGC
AACAATTACTTGTTAAACTCATTGAGAACGGTAAAGACTTCGATCTTGATGCTTATTACATTACACAGTTATTTCATACC
ATTATTGAAGATTCAGTTTTACTTCAGCAAGAATACTTCCAAAATCTAGCAAACCCTGACTTAAGTAGAAAACCCGTAGC
AAGAGTGGCTTACCTTGGCTCAAAAGGCTCATATTCAAATCTAGCAAGCCGTCGTTATTTCAGCAAAAAGAATATTGAAT
TAGCTGAGTTAGGCTGTGAAAACTTTAAAGAAGTCATTAAAACCGTTGAGTCAGGTCATGCTGATTACGGCGTATTGCCA
ATTGAAAATACAAGCTCAGGTTCGATTAACCAAGTTTATGATTTACTCCAACACACTAGCCTATACATTGTTGGGGAATT
AACTCAAAAAATTGATCATTGTTTATTAACAACGACTGAAACCTCTCTTGAATCAATTAAAACCTTGTATTCACACCCAC
AGCCACATGAACAATGTAGCGAATTTTTAAATCGACTCAATAATGTTGAGCTAATTTCTTGTGCAAGCACTGCTGATGCG
ATGATCACTGTAAAAGAATTAAACTCACCTGAAGTGGCAGCAATTGGTAACTCTGATAGTGGAAAGCTATATGGACTGCA
ATCCCTAATCACTAATATTTCGAATCAAACAGAAAACCAAACACGTTTTATCGTTGTTGCAAGAAAGCCTGTGGATGTAT
CAGAGCAAATTCCAGCTAAAACAACATTAATAATGTCCACAGCTCAAGATGCTGGTTCATTGGTTGAATCTCTATTGGTA
TTACAAAAATACGGCATCAATATGAGCAAGCTAGAATCTCGCCCTATTATGGGAAATCCTTGGGAAGAAATGTTCTATGT
TGATCTAGAAGCGCATCTAAAATCCGATGCTATGACTTCTGCTATCGAAGAATTAACGGCTATAACTCGCTATCTAAAAG
TACTTGGTTGCTATCCAATTGAAAATGTAGAACCAACAGCTATTCCATTATCAAATTGA

Upstream 100 bases:

>100_bases
ATTCTTAAGAACAGCGCTCATTTGAGCGCTGTTTTTTCTTGACGCAAACTAACCAATTCCTTATTGTGAATTAACTCTTA
ATTTACTAGGTTTGCATTTC

Downstream 100 bases:

>100_bases
TTACATCGAATAAAAGGTAAACATGAAAGTTATCATTGCTTCTCAAAACCCAGCGAAGATTGCCGCTGTTGAATCAGCAT
TTAATCTTGCGTTTCCTAAT

Product: fused chorismate mutase P/prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTDTHYSLDDIRLRLNELDNELLKLFSERRKLSLDVAKSKVKTAKPVRDPKREQQLLVKLIENGKDFDLDAYYITQLFHT
IIEDSVLLQQEYFQNLANPDLSRKPVARVAYLGSKGSYSNLASRRYFSKKNIELAELGCENFKEVIKTVESGHADYGVLP
IENTSSGSINQVYDLLQHTSLYIVGELTQKIDHCLLTTTETSLESIKTLYSHPQPHEQCSEFLNRLNNVELISCASTADA
MITVKELNSPEVAAIGNSDSGKLYGLQSLITNISNQTENQTRFIVVARKPVDVSEQIPAKTTLIMSTAQDAGSLVESLLV
LQKYGINMSKLESRPIMGNPWEEMFYVDLEAHLKSDAMTSAIEELTAITRYLKVLGCYPIENVEPTAIPLSN

Sequences:

>Translated_392_residues
MTDTHYSLDDIRLRLNELDNELLKLFSERRKLSLDVAKSKVKTAKPVRDPKREQQLLVKLIENGKDFDLDAYYITQLFHT
IIEDSVLLQQEYFQNLANPDLSRKPVARVAYLGSKGSYSNLASRRYFSKKNIELAELGCENFKEVIKTVESGHADYGVLP
IENTSSGSINQVYDLLQHTSLYIVGELTQKIDHCLLTTTETSLESIKTLYSHPQPHEQCSEFLNRLNNVELISCASTADA
MITVKELNSPEVAAIGNSDSGKLYGLQSLITNISNQTENQTRFIVVARKPVDVSEQIPAKTTLIMSTAQDAGSLVESLLV
LQKYGINMSKLESRPIMGNPWEEMFYVDLEAHLKSDAMTSAIEELTAITRYLKVLGCYPIENVEPTAIPLSN
>Mature_391_residues
TDTHYSLDDIRLRLNELDNELLKLFSERRKLSLDVAKSKVKTAKPVRDPKREQQLLVKLIENGKDFDLDAYYITQLFHTI
IEDSVLLQQEYFQNLANPDLSRKPVARVAYLGSKGSYSNLASRRYFSKKNIELAELGCENFKEVIKTVESGHADYGVLPI
ENTSSGSINQVYDLLQHTSLYIVGELTQKIDHCLLTTTETSLESIKTLYSHPQPHEQCSEFLNRLNNVELISCASTADAM
ITVKELNSPEVAAIGNSDSGKLYGLQSLITNISNQTENQTRFIVVARKPVDVSEQIPAKTTLIMSTAQDAGSLVESLLVL
QKYGINMSKLESRPIMGNPWEEMFYVDLEAHLKSDAMTSAIEELTAITRYLKVLGCYPIENVEPTAIPLSN

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=378, Percent_Identity=54.4973544973545, Blast_Score=414, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324013, Length=305, Percent_Identity=23.2786885245902, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 44031; Mature: 43899

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTHYSLDDIRLRLNELDNELLKLFSERRKLSLDVAKSKVKTAKPVRDPKREQQLLVKL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IENGKDFDLDAYYITQLFHTIIEDSVLLQQEYFQNLANPDLSRKPVARVAYLGSKGSYSN
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCCHHH
LASRRYFSKKNIELAELGCENFKEVIKTVESGHADYGVLPIENTSSGSINQVYDLLQHTS
HHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHCH
LYIVGELTQKIDHCLLTTTETSLESIKTLYSHPQPHEQCSEFLNRLNNVELISCASTADA
HEEEHHHHHHHHHHEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCC
MITVKELNSPEVAAIGNSDSGKLYGLQSLITNISNQTENQTRFIVVARKPVDVSEQIPAK
EEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCCCC
TTLIMSTAQDAGSLVESLLVLQKYGINMSKLESRPIMGNPWEEMFYVDLEAHLKSDAMTS
CEEEEECHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHEEEEHHHHHHHHHHHH
AIEELTAITRYLKVLGCYPIENVEPTAIPLSN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDTHYSLDDIRLRLNELDNELLKLFSERRKLSLDVAKSKVKTAKPVRDPKREQQLLVKL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IENGKDFDLDAYYITQLFHTIIEDSVLLQQEYFQNLANPDLSRKPVARVAYLGSKGSYSN
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCCHHH
LASRRYFSKKNIELAELGCENFKEVIKTVESGHADYGVLPIENTSSGSINQVYDLLQHTS
HHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHCH
LYIVGELTQKIDHCLLTTTETSLESIKTLYSHPQPHEQCSEFLNRLNNVELISCASTADA
HEEEHHHHHHHHHHEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCC
MITVKELNSPEVAAIGNSDSGKLYGLQSLITNISNQTENQTRFIVVARKPVDVSEQIPAK
EEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCCCC
TTLIMSTAQDAGSLVESLLVLQKYGINMSKLESRPIMGNPWEEMFYVDLEAHLKSDAMTS
CEEEEECHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHEEEEHHHHHHHHHHHH
AIEELTAITRYLKVLGCYPIENVEPTAIPLSN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1512561; 1444388 [H]