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The map label for this gene is lepA [H]

Identifier: 57651977

GI number: 57651977

Start: 1671897

End: 1673720

Strand: Reverse

Name: lepA [H]

Synonym: SACOL1641

Alternate gene names: 57651977

Gene position: 1673720-1671897 (Counterclockwise)

Preceding gene: 57651979

Following gene: 57651976

Centisome position: 59.58

GC content: 33.99

Gene sequence:

>1824_bases
ATGGATAATGAGCAACGCTTAAAAAGAAGAGAGAATATAAGGAATTTCTCGATTATAGCACATATTGACCACGGAAAATC
TACATTGGCTGATAGAATTTTAGAAAATACCAAATCAGTTGAAACAAGAGATATGCAAGATCAGTTACTAGATTCAATGG
ATTTAGAAAGAGAACGTGGTATTACAATCAAATTAAACGCAGTACGTTTAAAGTACGAAGCTAAAGATGGAAATACTTAT
ACATTCCATTTAATCGATACGCCTGGACACGTCGATTTTACATATGAAGTGTCACGTTCTTTGGCAGCTTGTGAGGGCGC
GATTTTAGTAGTAGATGCGGCTCAAGGTATCGAAGCACAAACATTAGCAAATGTTTATTTAGCATTAGATAATGAGTTAG
AGTTATTGCCTGTTATTAACAAAATTGATTTACCTGCTGCAGAACCTGAACGCGTGAAACAAGAAATTGAAGATATGATA
GGTTTAGACCAAGACGATGTTGTTTTAGCAAGTGCTAAATCTAACATTGGAATTGAAGAGATACTAGAGAAAATAGTTGA
AGTTGTGCCAGCTCCAGATGGTGACCCAGAAGCACCACTAAAAGCGTTAATATTTGATTCTGAGTATGATCCATATAGAG
GGGTAATTTCATCGATAAGAATTGTGGACGGTGTTGTTAAAGCCGGAGATAAAATTCGAATGATGGCCACTGGTAAAGAG
TTCGAAGTAACAGAAGTTGGAATTAATACACCTAAGCAGCTTCCAGTTGATGAATTAACAGTTGGTGATGTTGGTTATAT
TATTGCAAGTATTAAAAATGTTGATGATTCTAGGGTTGGTGACACCATCACATTAGCTAGTAGACCTGCATCAGAACCAT
TGCAAGGTTATAAGAAAATGAATCCAATGGTATATTGCGGACTGTTCCCAATAGATAACAAAAATTATAATGATTTAAGA
GAAGCATTAGAAAAATTACAATTGAATGATGCATCATTAGAATTTGAGCCTGAATCGTCACAAGCATTAGGTTTTGGTTA
TAGAACTGGTTTCTTAGGTATGTTACACATGGAAATAATTCAAGAAAGAATTGAAAGAGAATTTGGTATTGAATTAATTG
CAACTGCACCATCTGTAATTTATCAATGTGTTTTAAGGGACGGTTCAGAAGTGACGGTTGATAACCCAGCACAAATGCCA
GATCGTGATAAAATTGATAAAATATTTGAGCCATATGTTCGTGCAACTATGATGGTTCCAAATGACTATGTCGGTGCAGT
AATGGAATTATGTCAACGTAAACGTGGACAATTTATAAATATGGACTATTTAGATGATATTCGTGTAAATATTGTTTATG
AATTACCTTTAGCTGAAGTTGTATTTGATTTCTTCGATCAACTTAAATCTAATACTAAAGGATATGCATCATTTGATTAT
GAATTCATCGAAAATAAAGAAAGTAATTTAGTCAAGATGGATATTTTATTAAATGGTGATAAAGTGGATGCGCTAAGCTT
CATAGTTCATAGAGATTTTGCATATGAACGTGGTAAAGCATTAGTTGAAAAACTTAAAACGTTAATTCCAAGACAGCAAT
TTGAAGTACCTGTACAGGCTGCAATAGGACAAAAAATTGTAGCGCGTACAAATATTAAATCAATGGGTAAAAACGTTTTA
GCTAAATGTTATGGCGGTGACATAAGCCGTAAACGTAAATTACTTGAAAAACAAAAAGCAGGTAAAGCTAAGATGAAAGC
AGTTGGTAATGTTGAAATTCCACAAGATGCTTTCTTGGCTGTATTGAAAATGGATGATGAATAA

Upstream 100 bases:

>100_bases
ATCCAAGATTATGGCGTTTGCATTTACCTATTAAAAACGTTATTATATCAAAGATGCGAAAGATAATACGGGTTTATTTT
ATGAAAGTGAGAAGGATAAA

Downstream 100 bases:

>100_bases
TTTTAAAAAATCAATTAACAATTTACAATGAATAAAGTTTAATAACTAAAAAGAGGGAGCCTAGGATAAATTAACGTCCT
GGGCTTTACAATGTTATATT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRDMQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTY
TFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISSIRIVDGVVKAGDKIRMMATGKE
FEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDDSRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLR
EALEKLQLNDASLEFEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVTVDNPAQMP
DRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVNIVYELPLAEVVFDFFDQLKSNTKGYASFDY
EFIENKESNLVKMDILLNGDKVDALSFIVHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVL
AKCYGGDISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE

Sequences:

>Translated_607_residues
MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRDMQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTY
TFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISSIRIVDGVVKAGDKIRMMATGKE
FEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDDSRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLR
EALEKLQLNDASLEFEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVTVDNPAQMP
DRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVNIVYELPLAEVVFDFFDQLKSNTKGYASFDY
EFIENKESNLVKMDILLNGDKVDALSFIVHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVL
AKCYGGDISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE
>Mature_607_residues
MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRDMQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTY
TFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISSIRIVDGVVKAGDKIRMMATGKE
FEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDDSRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLR
EALEKLQLNDASLEFEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVTVDNPAQMP
DRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVNIVYELPLAEVVFDFFDQLKSNTKGYASFDY
EFIENKESNLVKMDILLNGDKVDALSFIVHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVL
AKCYGGDISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=50.2495840266223, Blast_Score=632, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=140, Percent_Identity=42.8571428571429, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI18390331, Length=253, Percent_Identity=32.0158102766798, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=37.5838926174497, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=45.5223880597015, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=45.5223880597015, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=45.5223880597015, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI217272894, Length=146, Percent_Identity=36.986301369863, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI217272892, Length=146, Percent_Identity=36.986301369863, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=41.7391304347826, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=41.7391304347826, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=41.7391304347826, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI53729339, Length=270, Percent_Identity=27.4074074074074, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI53729337, Length=270, Percent_Identity=27.4074074074074, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI34147630, Length=314, Percent_Identity=25.796178343949, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=56.9023569023569, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=532, Percent_Identity=29.3233082706767, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789738, Length=182, Percent_Identity=35.7142857142857, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1790835, Length=152, Percent_Identity=29.6052631578947, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=256, Percent_Identity=29.296875, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789737, Length=290, Percent_Identity=28.2758620689655, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790412, Length=290, Percent_Identity=28.2758620689655, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1789108, Length=282, Percent_Identity=24.822695035461, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=41.9934640522876, Blast_Score=502, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17556745, Length=475, Percent_Identity=24, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=41.0958904109589, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=166, Percent_Identity=36.144578313253, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=138, Percent_Identity=38.4057971014493, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=138, Percent_Identity=38.4057971014493, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=32.3308270676692, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=261, Percent_Identity=31.8007662835249, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=283, Percent_Identity=26.8551236749117, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=43.7603993344426, Blast_Score=514, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6323098, Length=189, Percent_Identity=36.5079365079365, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=37.5796178343949, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=37.5796178343949, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=42.8571428571429, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=162, Percent_Identity=35.8024691358025, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=245, Percent_Identity=28.1632653061224, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322675, Length=141, Percent_Identity=29.7872340425532, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=43.7603993344426, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582462, Length=205, Percent_Identity=37.0731707317073, Blast_Score=108, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=42.2535211267606, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24585709, Length=158, Percent_Identity=35.4430379746835, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585711, Length=158, Percent_Identity=35.4430379746835, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585713, Length=158, Percent_Identity=35.4430379746835, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=38.4105960264901, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363316, Length=300, Percent_Identity=30, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17864358, Length=300, Percent_Identity=30, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=31.8518518518519, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28572034, Length=221, Percent_Identity=28.0542986425339, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921738, Length=234, Percent_Identity=32.4786324786325, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 68176; Mature: 68176

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRDMQDQLLDSMDLERERG
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCC
ITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHH
TLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMIGLDQDDVVLASAKSNIGIEE
HHHEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHH
ILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISSIRIVDGVVKAGDKIRMMATGKE
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
FEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDDSRVGDTITLASRPASEPLQGYKKM
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHC
NPMVYCGLFPIDNKNYNDLREALEKLQLNDASLEFEPESSQALGFGYRTGFLGMLHMEII
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEECCHHHHHHHHHHHHHH
QERIEREFGIELIATAPSVIYQCVLRDGSEVTVDNPAQMPDRDKIDKIFEPYVRATMMVP
HHHHHHHHCEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEECC
NDYVGAVMELCQRKRGQFINMDYLDDIRVNIVYELPLAEVVFDFFDQLKSNTKGYASFDY
CHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCEEECH
EFIENKESNLVKMDILLNGDKVDALSFIVHRDFAYERGKALVEKLKTLIPRQQFEVPVQA
HHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHH
AIGQKIVARTNIKSMGKNVLAKCYGGDISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLA
HHCCHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHEEE
VLKMDDE
EEEECCC
>Mature Secondary Structure
MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRDMQDQLLDSMDLERERG
CCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCC
ITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCHH
TLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMIGLDQDDVVLASAKSNIGIEE
HHHEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHH
ILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISSIRIVDGVVKAGDKIRMMATGKE
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
FEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDDSRVGDTITLASRPASEPLQGYKKM
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHC
NPMVYCGLFPIDNKNYNDLREALEKLQLNDASLEFEPESSQALGFGYRTGFLGMLHMEII
CCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCCCEEECCHHHHHHHHHHHHHH
QERIEREFGIELIATAPSVIYQCVLRDGSEVTVDNPAQMPDRDKIDKIFEPYVRATMMVP
HHHHHHHHCEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEECC
NDYVGAVMELCQRKRGQFINMDYLDDIRVNIVYELPLAEVVFDFFDQLKSNTKGYASFDY
CHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCEEECH
EFIENKESNLVKMDILLNGDKVDALSFIVHRDFAYERGKALVEKLKTLIPRQQFEVPVQA
HHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHH
AIGQKIVARTNIKSMGKNVLAKCYGGDISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLA
HHCCHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHEEE
VLKMDDE
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA