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The map label for this gene is ycnB [H]

Identifier: 57650958

GI number: 57650958

Start: 2472216

End: 2473613

Strand: Reverse

Name: ycnB [H]

Synonym: SACOL2413

Alternate gene names: 57650958

Gene position: 2473613-2472216 (Counterclockwise)

Preceding gene: 57650960

Following gene: 57650957

Centisome position: 88.05

GC content: 34.12

Gene sequence:

>1398_bases
ATGGGTGGATTTTTTGGATTATTAAATGAAACACTATTAGTGACGGCTTTACCAAGTATTATGAAAGATTTTGAAATTTC
ATATACACAAGTTCAATGGCTGACAACAGCTTTTTTATTGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATAC
AACGTTATACAACAAGACAAGTGTTTTTAGTGGGTATTTCTATCTTTTTCTTAGGTACATTACTCGGCGGCTTGAGTCCG
CACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCGTTAGGCGCAGGTATTATGATGCCATTGATGATGACAACGAT
TTTGGATGTTTTCCAACCACATGAACGCGGTAAATATATGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATTG
GACCTACTCTTTCAGGTTACCTTGTTGAATATTTTAACTGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCAGCTGTG
ACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGAACTACAATTAAAGTACCTATTGATTTTATTTCTGTCATTTT
TTCTGTACTAGGTTTCGGCGGGTTATTGTATGGAACGAGTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTAG
TATCTATGATTGGAGGCGTTGTTTTAGTCGCATTATTTGTATTACGTCAATATCGGCTATCAACACCATTATTAAATTTT
GCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATTATTATGGGTGTCACAATGGTATCGATGATTGGTTCGGAAAC
GATTTTACCTATCTTTGTGCAAAATTTATTGCATCGTTCAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATTG
TTATGGCATTTATGTCGATGACTTCGGGTGCTTTATATGAAAAGTTTGGTCCTAGAAATCTTGCTTTAGTAGGTATGGCG
ATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGATGAACAAACATCAACAATCATGTTGGCAACAGTTTATGCGAT
TCGAATGGTTGGTATCGCGTTAGGATTAATTCCAGTAATGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCAC
ATGGTTCATCTATGACAAACACAGTACAACAAATTGCTGGTTCTATCGGAACAGCTGCATTAATTACTATTTTATCCCAT
GCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAATGGCATGAACAAAATTGATATGATGAATCAAATTAAAGTTGA
TACGATGTTGCATGGTTATCACGCTGGATTTTTATTTGCATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATGT
TACAAGGTAAGAAAAAAGAAGTTGATTCTCGTCAATAA

Upstream 100 bases:

>100_bases
ATTACCGGATATCTAAAAATGTGTGTCGTTTTTTAGATGGTGAGGGGGGAAGCTTTAAATGTCGAAGAAACAAAAATTAA
CGATGATTATTACTATGCTG

Downstream 100 bases:

>100_bases
ATATAAGTTGCTAGCTATATAAAGCTTTTTAGCAAAAGTTCAACATATTGACTTATCCGGCATTTCAGATTAAAATATTT
TTATTCCGATTAGAATAATA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSP
HFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAV
TFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNF
AVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSH
ASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Sequences:

>Translated_465_residues
MGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSP
HFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAV
TFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNF
AVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSH
ASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ
>Mature_464_residues
GGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPH
FATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVT
FLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFA
VFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAI
VVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHA
SKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=446, Percent_Identity=24.4394618834081, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788710, Length=407, Percent_Identity=24.8157248157248, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1790195, Length=408, Percent_Identity=25.4901960784314, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081983, Length=394, Percent_Identity=23.8578680203046, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788392, Length=401, Percent_Identity=23.4413965087282, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=26.7295597484277, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=138, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50541; Mature: 50410

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQ
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]