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The map label for this gene is fosB

Identifier: 57650875

GI number: 57650875

Start: 2388866

End: 2389285

Strand: Direct

Name: fosB

Synonym: SACOL2326

Alternate gene names: 57650875

Gene position: 2388866-2389285 (Clockwise)

Preceding gene: 57650874

Following gene: 57650877

Centisome position: 85.03

GC content: 29.05

Gene sequence:

>420_bases
ATGTTAAAATCTATTAATCATATATGCTTTTCAGTCAGAAATTTAAACGATTCAATACATTTTTATAGAGATATTTTACT
TGGGAAATTGCTATTGACTGGTAAAAAAACTGCTTATTTTGAGCTTGCAGGCCTATGGATTGCTTTAAATGAAGAAAAAG
ATATACCACGTAATGAAATTCACTTTTCATATACACATATAGCTTTCACTATAGATGACAGCGAATTTAAATATTGGCAT
CAGAGGTTAAAAGATAATAACGTGAATATTTTAGAAGGAAGAGTTAGAGATATTAGAGATAGACAATCAATTTACTTTAC
CGACCCTGATGGTCATAAGCTAGAATTACATACTGGCACACTTGAGAACAGATTAAATTATTATAAAGAGGCTAAACCAC
ATATGACATTTTACAAATAA

Upstream 100 bases:

>100_bases
ATGCAGTTGCGCAGATCATCGTATAAAAATTAATGACGTCATTTCAAAAATCGATACAAAAATAATTTATTATAAAAATT
CTAAGAAAGAAGTGAAGCAG

Downstream 100 bases:

>100_bases
GGTGTCATTATAAAAAGGCCTCTTGAACTCCGTTAAAATTTTAATTAATTATTATATAATAAGAGAACTTTTCAAACAAT
ACAGTTGTTATTTTTGCTAT

Product: fosfomycin resistance protein FosB

Products: NA

Alternate protein names: Fosfomycin resistance protein

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAGLWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWH
QRLKDNNVNILEGRVRDIRDRQSIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK

Sequences:

>Translated_139_residues
MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAGLWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWH
QRLKDNNVNILEGRVRDIRDRQSIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK
>Mature_139_residues
MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAGLWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWH
QRLKDNNVNILEGRVRDIRDRQSIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK

Specific function: Metallothiol transferase which confers resistance to fosfomycin by catalyzing the addition of a thiol cofactor to fosfomycin. L-cysteine is probably the physiological thiol donor

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family. FosB subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOSB_STAA1 (A7X5T8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442907.1
- ProteinModelPortal:   A7X5T8
- SMR:   A7X5T8
- STRING:   A7X5T8
- EnsemblBacteria:   EBSTAT00000003047
- GeneID:   5559219
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2317
- eggNOG:   COG0346
- GeneTree:   EBGT00050000025824
- HOGENOM:   HBG704761
- OMA:   QDRLNYY
- ProtClustDB:   PRK04101
- BioCyc:   SAUR418127:SAHV_2317-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01512
- InterPro:   IPR004360
- InterPro:   IPR022858

Pfam domain/function: PF00903 Glyoxalase

EC number: NA

Molecular weight: Translated: 16648; Mature: 16648

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAGLWIALNEEKDIPRNEI
CCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCE
HFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQSIYFTDPDGHKLELHTGT
EEEEEEEEEEEECCHHHHHHHHHCCCCCEEEECHHHCCCCCCCEEEECCCCCEEEEECCC
LENRLNYYKEAKPHMTFYK
HHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAGLWIALNEEKDIPRNEI
CCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCE
HFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQSIYFTDPDGHKLELHTGT
EEEEEEEEEEEECCHHHHHHHHHCCCCCEEEECHHHCCCCCCCEEEECCCCCEEEEECCC
LENRLNYYKEAKPHMTFYK
HHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA