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The map label for this gene is hemB

Identifier: 57650540

GI number: 57650540

Start: 1746055

End: 1747029

Strand: Reverse

Name: hemB

Synonym: SACOL1715

Alternate gene names: 57650540

Gene position: 1747029-1746055 (Counterclockwise)

Preceding gene: 57650541

Following gene: 57650539

Centisome position: 62.18

GC content: 35.49

Gene sequence:

>975_bases
ATGAAATTTGATAGACATAGAAGATTGAGATCATCAGCGACAATGAGAGATATGGTTAGAGAGAATCATGTAAGAAAAGA
AGATTTAATATATCCAATTTTTGTAGTTGAAAAAGACGATGTGAAAAAAGAAATTAAGTCATTGCCAGGTGTATACCAAA
TCAGTTTGAATTTACTTGAAAGTGAATTAAAAGAAGCTTATGACTTAGGCATACGTGCCATTATGTTTTTCGGTGTTCCA
AACTCAAAAGATGATATAGGTACTGGTGCATACATTCACGATGGTGTTATTCAACAGGCAACACGTATTGCTAAAAAAAT
GTATGATGACTTATTAATTGTTGCAGACACTTGTTTATGTGAATATACTGATCATGGTCATTGTGGCGTGATTGATGACC
ATACACATGACGTTGACAATGATAAATCATTGCCACTACTTGTTAAAACAGCAATTTCTCAAGTGGAAGCTGGTGCTGAT
ATTATTGCGCCAAGTAATATGATGGATGGTTTTGTTGCTGAAATTCGTCGTGGATTAGATGAAGCCGGCTATTACAATAT
TCCTATAATGAGTTATGGTGTCAAGTATGCATCAAGTTTCTTTGGACCTTTTAGAGATGCAGCAGATTCAGCGCCATCAT
TTGGGGATAGAAAAACGTATCAGATGGACCCTGCTAACCGTTTGGAAGCACTTCGTGAATTAGAAAGTGATCTTAAAGAA
GGGTGCGACATGATGATTGTTAAACCTGCTCTAAGTTATTTAGATATAGTTCGAGATGTTAAAAATCATACGAATGTTCC
AGTTGTTGCATATAATGTGAGTGGAGAATATAGTATGACTAAAGCAGCGGCACAAAATGGTTGGATAGATGAAGAACGTG
TCGTTATGGAACAAATGGTTTCAATGAAACGTGCAGGTGCTGATATGATTATTACGTATTTTGCAAAGGACATTTGTCGC
TATTTAGATAAATAA

Upstream 100 bases:

>100_bases
CAGCACGGACCATTAAATCATATCAACAACCAGTAACAATTGCAGAAATTCAAACACTCGAATCACTAATTGAAAAGATT
TTAGAAAGTAGGGGCTAAAA

Downstream 100 bases:

>100_bases
GGTTTTATATTTATGATTTTCCATAAACTGTAGGAGGAATTTACTTTATGAGATATACGAAATCAGAAGAAGCAATGAAG
GTTGCTGAAACTTTAATGCC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKEIKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVP
NSKDDIGTGAYIHDGVIQQATRIAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRKTYQMDPANRLEALRELESDLKE
GCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGEYSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICR
YLDK

Sequences:

>Translated_324_residues
MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKEIKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVP
NSKDDIGTGAYIHDGVIQQATRIAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRKTYQMDPANRLEALRELESDLKE
GCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGEYSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICR
YLDK
>Mature_324_residues
MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKEIKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVP
NSKDDIGTGAYIHDGVIQQATRIAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRKTYQMDPANRLEALRELESDLKE
GCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGEYSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICR
YLDK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family

Homologues:

Organism=Homo sapiens, GI189083849, Length=301, Percent_Identity=42.1926910299003, Blast_Score=258, Evalue=7e-69,
Organism=Escherichia coli, GI87081728, Length=314, Percent_Identity=49.3630573248408, Blast_Score=301, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6321398, Length=316, Percent_Identity=42.0886075949367, Blast_Score=252, Evalue=6e-68,
Organism=Drosophila melanogaster, GI21358291, Length=318, Percent_Identity=37.7358490566038, Blast_Score=231, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM2_STAA8 (Q2FXR3)

Other databases:

- EMBL:   U89396
- EMBL:   CP000253
- RefSeq:   YP_500277.1
- ProteinModelPortal:   Q2FXR3
- SMR:   Q2FXR3
- STRING:   Q2FXR3
- EnsemblBacteria:   EBSTAT00000028723
- GeneID:   3919690
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_01772
- eggNOG:   COG0113
- GeneTree:   EBGT00050000024030
- HOGENOM:   HBG285270
- OMA:   ADLCFCE
- ProtClustDB:   PRK09283
- BioCyc:   SAUR93061:SAOUHSC_01772-MONOMER
- InterPro:   IPR001731
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11458
- PIRSF:   PIRSF001415
- PRINTS:   PR00144

Pfam domain/function: PF00490 ALAD

EC number: =4.2.1.24

Molecular weight: Translated: 36583; Mature: 36583

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: ACT_SITE 195-195 ACT_SITE 248-248 BINDING 205-205 BINDING 217-217 BINDING 274-274 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKEIKSLPGVYQISLNLLE
CCCHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEEEEECHHHHHHHHHCCCEEEHHHHHHH
SELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATRIAKKMYDDLLIVADTCLC
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGADIIAPSNMMDGFVAEIRRGLD
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCHH
EAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRKTYQMDPANRLEALRELESDLKE
HCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHC
GCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGEYSMTKAAAQNGWIDEERVVMEQMV
CCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHH
SMKRAGADMIITYFAKDICRYLDK
HHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKEIKSLPGVYQISLNLLE
CCCHHHHHHHHHHHHHHHHHHHCCCHHHCEEEEEEEECHHHHHHHHHCCCEEEHHHHHHH
SELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATRIAKKMYDDLLIVADTCLC
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGADIIAPSNMMDGFVAEIRRGLD
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCHH
EAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRKTYQMDPANRLEALRELESDLKE
HCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHC
GCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGEYSMTKAAAQNGWIDEERVVMEQMV
CCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHH
SMKRAGADMIITYFAKDICRYLDK
HHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9358061