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The map label for this gene is spoT [H]

Identifier: 57238144

GI number: 57238144

Start: 1325180

End: 1327375

Strand: Reverse

Name: spoT [H]

Synonym: CJE1408

Alternate gene names: 57238144

Gene position: 1327375-1325180 (Counterclockwise)

Preceding gene: 57238145

Following gene: 57238143

Centisome position: 74.66

GC content: 30.74

Gene sequence:

>2196_bases
TTGAAACCAATCGATGAAGAATTATTGCTTGAACAATTAATCGATAATGTAAAAAATTGTAAAGATTTAGAAGCTGCTAA
GGCTTTGCTTTTCGAAATTTGTGGCCATGATGCTATTCTTGAAAAAGCGGTAGATTACTGTATATTTTGCCATGAAGGGC
AGTTTAGAAAAAGTGGAGAGCCTTATGCGGTACATCCTATTTTAGTAGCAACACTGGTTGGATTTTTAAGTGAAAATAAA
AGCAATATTTTAGCTGCTTTATTGCATGATGTGATCGAAGATACAAACTGTACTAAAGAAGAACTTAGAGAGCAGTTTGG
TTCTGAGGTTTTAAAACTTGTTTTAGGGCTTACTAAAATTATAGAAATTAGAGAAGATAATCTCATTTCATCAAAATCTA
AAAAAAGTTTAACTAAATCAGCCTTGACTTTCAGAAATATGCTTTTAGCAAGCATTGAAGATGTTGGGGTTTTGATTGTG
AAGTTATGTGATAGGCTGCATAATATGCTTACTCTTGATATTCTAAGAGAAGATAAGCAAAAAAGAATCAGCGAAGAAAC
TTTAGTTGTATATGCCCCAATAGCCCATAGACTTGGTATTTCAAGCATAAAAAATTATTTGGAAGATCTTAGTTTTAAAT
ATCTTATGCCAGATGAGTATAAGCTTATTGACAATTATATCAACTCCAATAATCAAGAAATGCAATTAGGTTTAAATGAA
TTTATTTCCAAAATCGAACTTTTATTTTTGAGCAATGGCTTTAGACAAGGTAGTTTTGAAATTCAAAAACGCATTAAACA
TAGCTATTCTATTTATCTTAAAATGCAAAGAAAAGGCATTGGTATTGAAGAAGTTCTTGATTTGCTTGGGGTAAGAATTT
TGGTTGAAAAAGTAAGTGATTGTTATTTAGCTTTGGGAATTTTACATACACATTTTAATCCCTTGGTTTCGCGTTTTAAG
GATTATATAGCCTTGCCTAAACAAAATGGTTATCAAACCATACATACCACACTTTTTGATGCTAAAAGTATTATAGAAGC
ACAAATTCGAACTTTTGATATGCATAAAATTGCGGAATTTGGTATAGCAGCGCATTGGAAATATAAAGAAGATGGCAGTG
TGGTTGCGCCTCGTCTTGATTGGCTTACAGATATTTCAATGCAAAGTATAAACAATCTTGAAAATGCAGAAGATTATAAT
GCTATTGAACTTTATGAATATGCTAAAGATAGTCTTTATGTTGAAGATGTCGCAGTTTATTCTCCTAAGGGAGAAATTTT
CACTTTGCCTCGTGGTGCAACGGTTCTTGATTTTGCTTATGAAGTGCATACCAAAGTAGGGCTTCATGCAAAAAGTGCTT
ATGTAAATCGTATTAAAGTGCCTTTGTTAACCGAGCTTAAAAATGGCGATATTGTGCGAGTGGTTACTAGCAATGATAAA
TTTTATCGCTGTTCTTGGATAGATAGTGTTAAAACAGGAAAAGCAAAAGCAAGTATAAGGGAATTTTGTAAGCAAAAAAT
CAGAGAGATTAATCTAGCAAGTTCTATTAATATGTTAAGTTTTATTTTTGGAGTTGATAAAAACAGAATTGAAACTTGGA
TAGAAAAAGAAAATTTAGCCAGACGTATTAGACAAGTAGCAACAGATAGTGCTTATTTGAAAGATATAGTTAATGGTCTT
AAAAAATACGCCAAGAAGTCATATTGGTTTGATAAATATGAGATTAAAGAACAAAAAATAGGAAATTTTACTCTATATTG
TAACCATAAAATCGCTAATGTGGATTTTGATTTTTGCTGCCATCCAAAAAGAGGTGATGCTGTTTTAGCTTTTGTTAAAG
GTGGTAACGCTATTGTCCATCATAAGCTTTGCGATAGAGCTGATAAAATGATAGATAATAATCAAGATATGGTATTTATA
AAATGGGATTCTAACATCCCTAAATCTTATAAATTGATTTTTTCTTTGGAAAATAAAAAAGGTGTTTTAGCGGAATTTTT
GGCATTTTTAGCTAAAATGCAGATAAATTTATTAACAATTAATTTATCAAGTGATTTAAATTCGGCGGTAGATTATTTTG
AGATTACAATGGAAATTCCTGATAATATCAATCCAGATAGTGTCAAGGATAGGTTAAAATCTCGTTGTAAAATTTTAGAT
TTTACATCTTTAAATGATGCTTATAAAGAAGGTTAA

Upstream 100 bases:

>100_bases
GGAGCAACCCCTTTGGTAGACTTTGATAAAAATAAAAATAAACTTGCAGATATAGCTTTATATGAAATTGCAGAAAATAA
AATCACTTTAGAGGGTTTAG

Downstream 100 bases:

>100_bases
AAAGGTTTATTATGGATATAAAAAAAATATTAGCAGAAGTTAAGCGTGGTTGTGCAGAGCTTATTGATGAAGAAAGAATA
GAAAAATTAATTAAAAATTA

Product: RelA/SpoT family protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MKPIDEELLLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPILVATLVGFLSENK
SNILAALLHDVIEDTNCTKEELREQFGSEVLKLVLGLTKIIEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIV
KLCDRLHNMLTLDILREDKQKRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNE
FISKIELLFLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGILHTHFNPLVSRFK
DYIALPKQNGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYN
AIELYEYAKDSLYVEDVAVYSPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDK
FYRCSWIDSVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVATDSAYLKDIVNGL
KKYAKKSYWFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFI
KWDSNIPKSYKLIFSLENKKGVLAEFLAFLAKMQINLLTINLSSDLNSAVDYFEITMEIPDNINPDSVKDRLKSRCKILD
FTSLNDAYKEG

Sequences:

>Translated_731_residues
MKPIDEELLLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPILVATLVGFLSENK
SNILAALLHDVIEDTNCTKEELREQFGSEVLKLVLGLTKIIEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIV
KLCDRLHNMLTLDILREDKQKRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNE
FISKIELLFLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGILHTHFNPLVSRFK
DYIALPKQNGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYN
AIELYEYAKDSLYVEDVAVYSPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDK
FYRCSWIDSVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVATDSAYLKDIVNGL
KKYAKKSYWFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFI
KWDSNIPKSYKLIFSLENKKGVLAEFLAFLAKMQINLLTINLSSDLNSAVDYFEITMEIPDNINPDSVKDRLKSRCKILD
FTSLNDAYKEG
>Mature_731_residues
MKPIDEELLLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPILVATLVGFLSENK
SNILAALLHDVIEDTNCTKEELREQFGSEVLKLVLGLTKIIEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIV
KLCDRLHNMLTLDILREDKQKRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNE
FISKIELLFLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGILHTHFNPLVSRFK
DYIALPKQNGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYN
AIELYEYAKDSLYVEDVAVYSPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDK
FYRCSWIDSVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVATDSAYLKDIVNGL
KKYAKKSYWFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFI
KWDSNIPKSYKLIFSLENKKGVLAEFLAFLAKMQINLLTINLSSDLNSAVDYFEITMEIPDNINPDSVKDRLKSRCKILD
FTSLNDAYKEG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=651, Percent_Identity=34.1013824884793, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789147, Length=679, Percent_Identity=28.7187039764359, Blast_Score=278, Evalue=7e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 83736; Mature: 83736

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIDEELLLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGE
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCC
PYAVHPILVATLVGFLSENKSNILAALLHDVIEDTNCTKEELREQFGSEVLKLVLGLTKI
CEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIVKLCDRLHNMLTLDILREDKQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNE
HHCCCCEEEEEECHHHHCCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCEEHHCHHH
FISKIELLFLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSD
HHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
CYLALGILHTHFNPLVSRFKDYIALPKQNGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYNAIELYEYAKDSLYVEDVAVY
CCEEEEEECCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEHEEEE
SPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDK
CCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHEECCCCCEEEEEECCCC
FYRCSWIDSVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLA
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RRIRQVATDSAYLKDIVNGLKKYAKKSYWFDKYEIKEQKIGNFTLYCNHKIANVDFDFCC
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCEEECCCCCEEC
HPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFIKWDSNIPKSYKLIFSLENKK
CCCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCC
GVLAEFLAFLAKMQINLLTINLSSDLNSAVDYFEITMEIPDNINPDSVKDRLKSRCKILD
CHHHHHHHHHHHCEEEEEEEEECCCHHHHHEEEEEEEECCCCCCHHHHHHHHHHHCEEEE
FTSLNDAYKEG
ECCCCHHHCCC
>Mature Secondary Structure
MKPIDEELLLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGE
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCC
PYAVHPILVATLVGFLSENKSNILAALLHDVIEDTNCTKEELREQFGSEVLKLVLGLTKI
CEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIVKLCDRLHNMLTLDILREDKQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNE
HHCCCCEEEEEECHHHHCCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCEEHHCHHH
FISKIELLFLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSD
HHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
CYLALGILHTHFNPLVSRFKDYIALPKQNGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYNAIELYEYAKDSLYVEDVAVY
CCEEEEEECCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEHEEEE
SPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDK
CCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHEECCCCCEEEEEECCCC
FYRCSWIDSVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLA
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RRIRQVATDSAYLKDIVNGLKKYAKKSYWFDKYEIKEQKIGNFTLYCNHKIANVDFDFCC
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCEEECCCCCEEC
HPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFIKWDSNIPKSYKLIFSLENKK
CCCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCC
GVLAEFLAFLAKMQINLLTINLSSDLNSAVDYFEITMEIPDNINPDSVKDRLKSRCKILD
CHHHHHHHHHHHCEEEEEEEEECCCHHHHHEEEEEEEECCCCCCHHHHHHHHHHHCEEEE
FTSLNDAYKEG
ECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]