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The map label for this gene is tyrS [H]

Identifier: 57238143

GI number: 57238143

Start: 1323963

End: 1325168

Strand: Reverse

Name: tyrS [H]

Synonym: CJE1407

Alternate gene names: 57238143

Gene position: 1325168-1323963 (Counterclockwise)

Preceding gene: 57238144

Following gene: 57238142

Centisome position: 74.54

GC content: 32.42

Gene sequence:

>1206_bases
ATGGATATAAAAAAAATATTAGCAGAAGTTAAGCGTGGTTGTGCAGAGCTTATTGATGAAGAAAGAATAGAAAAATTAAT
TAAAAATTATTATGAAAAGGGTGAAAATTTCTTTATAAAGGCGGGGTTTGATCCTACAGCGCCTGATTTACATTTAGGTC
ATAGCGTGGTTTTAACAAAAATGGCTTTTTTGCAAAAACATGGAGCTATTGTGCAGTTTTTGATTGGAGATTTTACAGGA
CAAATTGGCGATCCAAGTGGAAAAAGTGCAACGCGTAAAAAGCTGGATAAAGAGCAAGTTTTAATCAATGCTAAAACTTA
TGAAACGCAGGTTTTTAAGGTTTTGGATAAAGAAAAAACACAGATTAAGTTTAATTCTACTTGGCTTAATGAACTTGGAG
CTGCGGGTATAGTAGAGCTTACTTCAACTTTTAGTGTAGCTAGAATGCTTGAGCGTGACGATTTTACCAAGAGATTTAAA
GAGCAAAGTCCTATTTCGATTTGCGAGTTTTTGTATCCTTTACTTCAAGGATATGATAGTGTGGCTTTAAAAAGCGATAT
TGAAATGGGTGGAACGGATCAAAAATTTAATCTTTTGATGGGAAGACAGCTTCAAAGAGTTTATAACATAGGAAAAGAAC
AAGCTGTGATTATGATGCCTTTACTCGAAGGTTTAGACGGTGTAAACAAAATGAGTAAGAGTTTAAATAATTACATTGGT
GTTACAGAAAAAGCTAATGATATGTATGCTAAAATTTTAAGTATTAGTGATGAATTGATGTTTAGATATTATGAACTTTT
AAGTCAAAAAAGTCTAGAAGAAATTGCGCAAATAAAAAAAGATATAGAACAAAGCAATTTACATCCTAAAAAAGCTAAAG
AAAATTTAGCTTTAGAGATTACAGAGCGTTTTCACTCCAAAGAAGAGGCTAATAATGCTAAGTCTGAATTTGATAGAATT
CATTCTCAAAATGCTTTGCCAAGTGATATGGCTGAGTTTGAAATACAAGGAAAAATTTGGCTTGCTAAGGCTTTGGTTGA
ATGTGGTTTGGAAAGTTCTACTTCTGCGGCAAGAAGAAGCATCAGTGCTAATGCAGTAAGTGTAAATTCTCAAAAAGTTA
GCGACGAGCAAATGTATTTGAAACAAGGAGAATACATCTTACAAATAGGTAAAAGAAAATTTGCAAAATTAAAGGTAAAA
GAATGA

Upstream 100 bases:

>100_bases
TCAATCCAGATAGTGTCAAGGATAGGTTAAAATCTCGTTGTAAAATTTTAGATTTTACATCTTTAAATGATGCTTATAAA
GAAGGTTAAAAAGGTTTATT

Downstream 100 bases:

>100_bases
ATTTACAACCCTTACAAATAGGAAAACATACTATAAAATATCCCATTTTTCAAGGGGGAATGGGACTAGGCATAAGCTGG
GATAGATTAGCTTCTGCAGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTKMAFLQKHGAIVQFLIGDFTG
QIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKTQIKFNSTWLNELGAAGIVELTSTFSVARMLERDDFTKRFK
EQSPISICEFLYPLLQGYDSVALKSDIEMGGTDQKFNLLMGRQLQRVYNIGKEQAVIMMPLLEGLDGVNKMSKSLNNYIG
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQSNLHPKKAKENLALEITERFHSKEEANNAKSEFDRI
HSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRSISANAVSVNSQKVSDEQMYLKQGEYILQIGKRKFAKLKVK
E

Sequences:

>Translated_401_residues
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTKMAFLQKHGAIVQFLIGDFTG
QIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKTQIKFNSTWLNELGAAGIVELTSTFSVARMLERDDFTKRFK
EQSPISICEFLYPLLQGYDSVALKSDIEMGGTDQKFNLLMGRQLQRVYNIGKEQAVIMMPLLEGLDGVNKMSKSLNNYIG
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQSNLHPKKAKENLALEITERFHSKEEANNAKSEFDRI
HSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRSISANAVSVNSQKVSDEQMYLKQGEYILQIGKRKFAKLKVK
E
>Mature_401_residues
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTKMAFLQKHGAIVQFLIGDFTG
QIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKTQIKFNSTWLNELGAAGIVELTSTFSVARMLERDDFTKRFK
EQSPISICEFLYPLLQGYDSVALKSDIEMGGTDQKFNLLMGRQLQRVYNIGKEQAVIMMPLLEGLDGVNKMSKSLNNYIG
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQSNLHPKKAKENLALEITERFHSKEEANNAKSEFDRI
HSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRSISANAVSVNSQKVSDEQMYLKQGEYILQIGKRKFAKLKVK
E

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=431, Percent_Identity=27.1461716937355, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1787925, Length=393, Percent_Identity=29.2620865139949, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=291, Percent_Identity=27.1477663230241, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=25.0569476082005, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130383, Length=442, Percent_Identity=25.3393665158371, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45465; Mature: 45465

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
MAFLQKHGAIVQFLIGDFTGQIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKT
HHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHEEEECHHHHHHHHHHHHCHHH
QIKFNSTWLNELGAAGIVELTSTFSVARMLERDDFTKRFKEQSPISICEFLYPLLQGYDS
EEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
VALKSDIEMGGTDQKFNLLMGRQLQRVYNIGKEQAVIMMPLLEGLDGVNKMSKSLNNYIG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCHHHHHHHHHHHHCC
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQSNLHPKKAKENLALEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
TERFHSKEEANNAKSEFDRIHSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCHHHHHHHHHHHCCCCCHHHHHHH
ISANAVSVNSQKVSDEQMYLKQGEYILQIGKRKFAKLKVKE
CCCCEEECCCHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
MAFLQKHGAIVQFLIGDFTGQIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKT
HHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHEEEECHHHHHHHHHHHHCHHH
QIKFNSTWLNELGAAGIVELTSTFSVARMLERDDFTKRFKEQSPISICEFLYPLLQGYDS
EEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
VALKSDIEMGGTDQKFNLLMGRQLQRVYNIGKEQAVIMMPLLEGLDGVNKMSKSLNNYIG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCHHHHHHHHHHHHCC
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQSNLHPKKAKENLALEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
TERFHSKEEANNAKSEFDRIHSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCHHHHHHHHHHHCCCCCHHHHHHH
ISANAVSVNSQKVSDEQMYLKQGEYILQIGKRKFAKLKVKE
CCCCEEECCCHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]