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The map label for this gene is hydA [H]

Identifier: 57238139

GI number: 57238139

Start: 1317798

End: 1318880

Strand: Reverse

Name: hydA [H]

Synonym: CJE1403

Alternate gene names: 57238139

Gene position: 1318880-1317798 (Counterclockwise)

Preceding gene: 57238140

Following gene: 57238138

Centisome position: 74.18

GC content: 38.6

Gene sequence:

>1083_bases
TTGAAAGACAATGATAGTATTACAAAAGCATTAGAAAAGTCAGGTTTTTCTAGAAGGGATTTTATGAAATGGGCAGGTGC
TATGACGGCATTTTTGGCTTTGCCAGCGAGTTTTACTCCTATGGTGGCAAAAGCTGCTGAGCTTGCTGATAGGCTTCCTG
TTGTGTGGCTTCATATGGCTGAGTGTACAGGCTGTAGTGAGAGTTTGTTAAGAAGTGATACTCCAACGATTGATAGTTTG
ATTTTTGATTATATTTCTTTAGAATATCACGAAACAGTGATGGCTGCAGCAGGTTGGCAAGCTGAAGAAAATTTAGAAAG
TGCCATTCAAAAGCATAAAAATAAATACATTTTAATGGTTGAAGGTGGTATCCCTATGGATGATACGGAGCATTTTTTAA
CCATAGGTGCACATGGAAAAACAGGCTATGAGCTTTCAAAAATGGCAAGTGAAAATGCTTTGGCGATTTTTGCTATAGGA
ACCTGTTCTAGCTTTGGAGGAATTCAAGCCGCAAGACCAAATCCTAGCAACGCGCAGCCTTTAAGCAAAGTAACAAGTAA
AACTGTGATCAATGTTCCAGGCTGTCCTCCAAGTGAAAAAAATATAGTGGGTAATGTACTTCATTATTTATTATTTGGTG
AGTTACCAGCACTTGATGTGTATAATAGACCAAAATGGGCTTATGGTTTAAGAATTCATGATCTTTGTGAAAGACGCGGA
CATTTTGATGCGGGTGAATTTGTACATGCTTTTGGAGATGAAGGAGCAAAACAAGGTTTTTGTCTTTATAAAGTAGGTTG
TAAAGGACCTTATACTTTTAATAACTGCTCAAGAGAGAGATTTAACCAGCACACTTCTTGGCCTATTCAAGCAGGGCATG
GTTGTATAGGTTGTTCTGAGCCTAATTTTTGGGATACTATGGGACCTTTTGAAGAGCCTATGGCTAGTCGTAAATTTGAT
ACTGTTTTTGGTTTGGGTGCGGATAGTGTTTCAGATAAAATCGGTATAGGTGTGCTTACACTCACAGGCGTTGCTATAGC
AGCACACGCAGTTATATCTTCCATGCAAAAAGATAAGGAATAA

Upstream 100 bases:

>100_bases
TATTATTTATAGAGAATAAATTTCATGTTATAAAGGAAATATAATGATTGATTATCATCAAATAGAGTCGCGTTTATCGG
CTCTTGAAAAGCTTCCTTCT

Downstream 100 bases:

>100_bases
AAATGAGTCAAAAAATAATCGTAGATCCTATTACAAGAATTGAAGGGCATTTAAGAGTTGAAGTTGTTGTTGATGATAAC
AATGTGGTAAAAGAAGCTTA

Product: quinone-reactive Ni/Fe-hydrogenase, small subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKDNDSITKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMAECTGCSESLLRSDTPTIDSL
IFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMVEGGIPMDDTEHFLTIGAHGKTGYELSKMASENALAIFAIG
TCSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVYNRPKWAYGLRIHDLCERRG
HFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSEPNFWDTMGPFEEPMASRKFD
TVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE

Sequences:

>Translated_360_residues
MKDNDSITKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMAECTGCSESLLRSDTPTIDSL
IFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMVEGGIPMDDTEHFLTIGAHGKTGYELSKMASENALAIFAIG
TCSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVYNRPKWAYGLRIHDLCERRG
HFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSEPNFWDTMGPFEEPMASRKFD
TVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE
>Mature_360_residues
MKDNDSITKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMAECTGCSESLLRSDTPTIDSL
IFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMVEGGIPMDDTEHFLTIGAHGKTGYELSKMASENALAIFAIG
TCSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVYNRPKWAYGLRIHDLCERRG
HFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSEPNFWDTMGPFEEPMASRKFD
TVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=340, Percent_Identity=45, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=45.8904109589041, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 39302; Mature: 39302

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00024 HEMOPEXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNDSITKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEHH
ECTGCSESLLRSDTPTIDSLIFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMV
HHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
EGGIPMDDTEHFLTIGAHGKTGYELSKMASENALAIFAIGTCSSFGGIQAARPNPSNAQP
ECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCH
LSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVYNRPKWAYGLRIHDLCERRG
HHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHCC
HFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSE
CCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCC
PNFWDTMGPFEEPMASRKFDTVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE
CCCCCCCCCHHHHHHHCCCHHHEECCCCHHCHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDNDSITKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEHH
ECTGCSESLLRSDTPTIDSLIFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMV
HHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
EGGIPMDDTEHFLTIGAHGKTGYELSKMASENALAIFAIGTCSSFGGIQAARPNPSNAQP
ECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCH
LSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVYNRPKWAYGLRIHDLCERRG
HHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHHHHHHCC
HFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSE
CCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCC
PNFWDTMGPFEEPMASRKFDTVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE
CCCCCCCCCHHHHHHHCCCHHHEECCCCHHCHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]