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The map label for this gene is hydB [H]

Identifier: 57238138

GI number: 57238138

Start: 1316080

End: 1317795

Strand: Reverse

Name: hydB [H]

Synonym: CJE1402

Alternate gene names: 57238138

Gene position: 1317795-1316080 (Counterclockwise)

Preceding gene: 57238139

Following gene: 57238137

Centisome position: 74.12

GC content: 35.31

Gene sequence:

>1716_bases
ATGAGTCAAAAAATAATCGTAGATCCTATTACAAGAATTGAAGGGCATTTAAGAGTTGAAGTTGTTGTTGATGATAACAA
TGTGGTAAAAGAAGCTTATGCGGGCTCTACTTTGTGGCGTGGTATTGAAACCATAGTAAAAGGGCGTGATCCAAGAGATG
CGGGTTTTATGACGCAAAGAATTTGCGGAGTTTGTACTTTTTCACATTATAAAGCAGGTATAGTTGCTGTTGAAAATGCT
TTAGGTATTACTCCGCCTTTAAATGCGCTTTTAACAAGAACTTTAATGAATGCGGCTTTATTTTTACACGATCATATTGT
GCATTTTTATCAACTTCATGGGCTTGATTGGGCTGATGTGGTAAGTGCTTTAAGTGCTGATGTTAAAAAAGCAAGCGATG
AAGCTTTTAAATACACTTCAAATCCTTATGCAACAGGTGCGGATAAACTTCTTGAAGTGCAACAAAGACTTAAAACTTTT
GTAGATAAAGGAAATTTAGGACCTTTTGCTAATGCGTACTATGGTCATCCAACTTATCGTTTAAGTCCTGAGCAAAATTT
AATCGTTCTTTCTCACTATCTAGAATGTTTAAGAATTCAAAGAATCATTGCTCAATGTATGGCGATTTTTGGAGCTAAAA
ATCCACATCCACAAAGCTTAACCGTTGGTGGTGTAACTTGCGTTATGGATTTACTCGATCCTGCAAGAATGGGTGAATAT
ATGGTTAAATTCCAAGAAGTGCAAGACTTTGTTAATCGTGCGTATTATCCTGATCTTGTGATGGTAGGAAAAGCTTATGC
ACATGAAGCAAGTGTTTTAAATGATATAGGGGTAAATAATCTTTATACCTTTAAAGAATTCCAAATCGGAAGAGATGAAT
GGCTTTTTGAAAGTGGTATTATCAAAAATGGAGATTTAAGCAAGGTTTATGAAGTAGAAGAAGATAAGATTACTGAAGAG
GCAACTCATTCTTGGTATGCAGACAATGAACCTTTACATCCTTATGACGGCAAAACAAATCCAAATTATACAGGACTTGT
AGATGGGGAAAGCGTAGATCATCATGGAAATAATGTTCATTCTAAAGTGTTTGATACTAAGGGTAAATATAGTTGGATTA
AAGCTCCACGCTATGAAGGAAACCCTATGCAAGTAGGACCTTTAGCAAATATCGTTGTAAATTATGCTAAAGGTAATCAA
AATGTTGTTCCTGTAGTAGATGAGTTCTTAAAAGAAACAGGACTTCCTTTAAATGCTGTATTTAGTACACTTGGAAGAAC
AGCTGCTCGTTGTATTGAAGCAAAAATCGTTGCAAATAATGCTTTAAAAGCGTTTAATAATTTAGTTGAAAATTTAAAAG
TAGACCAGAGTACTTGTGCTCCTTATGTGATCGATAATTCTAAAGAATACAAAGGACGCTATATGGGGCATGTGCCACGC
GGAACATTAAGCCATTGGTGTAGAATTAAAAATGGTGTTATTGAAAATTGGCAAGCTGTGGTGCCTTCTACTTGGAATGC
AAGTCCAAAAGACGCAAATGGCGTAGGTGGAAGTTATGAGCAATGTTTGATAGGACTTAAAATCGCTGATGTAAAACAAC
CACTTGAAATCATTAGAAAAATTCACTCTTATGATCCTTGTATTGCTTGTGCGGTGCATGTAATGGATACTAAGGGCAAT
AATCTAAGTGAATATAAAGTGAATGTCAATTTATAA

Upstream 100 bases:

>100_bases
GTGTTTCAGATAAAATCGGTATAGGTGTGCTTACACTCACAGGCGTTGCTATAGCAGCACACGCAGTTATATCTTCCATG
CAAAAAGATAAGGAATAAAA

Downstream 100 bases:

>100_bases
GGAGGCTTTCATGCAAAACAAAGAAGAAAAATTGCAAAGAAAAGCTGAATATGAATTTAGCATAGGTTTGCGTTTAACGC
ACTGGGTAAGAGCTATTGCT

Product: quinone-reactive Ni/Fe-hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSQKIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQRICGVCTFSHYKAGIVAVENA
LGITPPLNALLTRTLMNAALFLHDHIVHFYQLHGLDWADVVSALSADVKKASDEAFKYTSNPYATGADKLLEVQQRLKTF
VDKGNLGPFANAYYGHPTYRLSPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
MVKFQEVQDFVNRAYYPDLVMVGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGIIKNGDLSKVYEVEEDKITEE
ATHSWYADNEPLHPYDGKTNPNYTGLVDGESVDHHGNNVHSKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNQ
NVVPVVDEFLKETGLPLNAVFSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRKIHSYDPCIACAVHVMDTKGN
NLSEYKVNVNL

Sequences:

>Translated_571_residues
MSQKIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQRICGVCTFSHYKAGIVAVENA
LGITPPLNALLTRTLMNAALFLHDHIVHFYQLHGLDWADVVSALSADVKKASDEAFKYTSNPYATGADKLLEVQQRLKTF
VDKGNLGPFANAYYGHPTYRLSPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
MVKFQEVQDFVNRAYYPDLVMVGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGIIKNGDLSKVYEVEEDKITEE
ATHSWYADNEPLHPYDGKTNPNYTGLVDGESVDHHGNNVHSKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNQ
NVVPVVDEFLKETGLPLNAVFSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRKIHSYDPCIACAVHVMDTKGN
NLSEYKVNVNL
>Mature_570_residues
SQKIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQRICGVCTFSHYKAGIVAVENAL
GITPPLNALLTRTLMNAALFLHDHIVHFYQLHGLDWADVVSALSADVKKASDEAFKYTSNPYATGADKLLEVQQRLKTFV
DKGNLGPFANAYYGHPTYRLSPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEYM
VKFQEVQDFVNRAYYPDLVMVGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGIIKNGDLSKVYEVEEDKITEEA
THSWYADNEPLHPYDGKTNPNYTGLVDGESVDHHGNNVHSKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNQN
VVPVVDEFLKETGLPLNAVFSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPRG
TLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRKIHSYDPCIACAVHVMDTKGNN
LSEYKVNVNL

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=588, Percent_Identity=40.8163265306122, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1789368, Length=591, Percent_Identity=41.4551607445008, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 63613; Mature: 63482

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQR
CCCCEEECCHHHCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
ICGVCTFSHYKAGIVAVENALGITPPLNALLTRTLMNAALFLHDHIVHFYQLHGLDWADV
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VSALSADVKKASDEAFKYTSNPYATGADKLLEVQQRLKTFVDKGNLGPFANAYYGHPTYR
HHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
LSPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCHHHHHHH
MVKFQEVQDFVNRAYYPDLVMVGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHCCC
IKNGDLSKVYEVEEDKITEEATHSWYADNEPLHPYDGKTNPNYTGLVDGESVDHHGNNVH
CCCCCCHHHEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH
SKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNQNVVPVVDEFLKETGLPLNAV
HHHHCCCCCEEEEECCCCCCCCEEECCHHHHHEEECCCCCCCHHHHHHHHHHCCCCHHHH
FSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCHHHCCCCCCCCCC
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRK
HHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHHHHH
IHSYDPCIACAVHVMDTKGNNLSEYKVNVNL
HHCCCCHHHHHEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure 
SQKIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQR
CCCEEECCHHHCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
ICGVCTFSHYKAGIVAVENALGITPPLNALLTRTLMNAALFLHDHIVHFYQLHGLDWADV
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VSALSADVKKASDEAFKYTSNPYATGADKLLEVQQRLKTFVDKGNLGPFANAYYGHPTYR
HHHHHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
LSPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCHHHHHHH
MVKFQEVQDFVNRAYYPDLVMVGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHCCC
IKNGDLSKVYEVEEDKITEEATHSWYADNEPLHPYDGKTNPNYTGLVDGESVDHHGNNVH
CCCCCCHHHEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH
SKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNQNVVPVVDEFLKETGLPLNAV
HHHHCCCCCEEEEECCCCCCCCEEECCHHHHHEEECCCCCCCHHHHHHHHHHCCCCHHHH
FSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCHHHCCCCCCCCCC
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRK
HHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCHHHHEECCCHHHHHHHHHHHHH
IHSYDPCIACAVHVMDTKGNNLSEYKVNVNL
HHCCCCHHHHHEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]