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The map label for this gene is waaA [H]

Identifier: 57237009

GI number: 57237009

Start: 742568

End: 743725

Strand: Direct

Name: waaA [H]

Synonym: CJE0807

Alternate gene names: 57237009

Gene position: 742568-743725 (Clockwise)

Preceding gene: 57237008

Following gene: 57237010

Centisome position: 41.77

GC content: 25.99

Gene sequence:

>1158_bases
TTGATCTTTTTTTATTATTTTTTAACTTGGACGGCATTTTTATTTTGTGCTGTCTTTATTCTTTTGCTTTCTTTTTTAAA
ATCCAAATACAAAACTAGTTTAAAATCCCGATTTTTTCTTTATAAAAATTTACATCAAGAAAAAGCTGATGTACATTTTC
ATGCTTGTTCTTACGGGGAGGTAAGAAGTATTAAAACTTTGGTTTTGAAATTTGATTCAAGAATAACTACTATTACTCAA
ACAGGTTTTGAATGTGCAAAAGAATTTTGTAAAAAGGTAAATTATCTTGCTTTTGAAAATTTTTTGCCTTTTTGGTTTAA
ACCTTGTAAGGTTTTGGTAATTTTTGAAGCAGAATATTGGCTTATGCTTGTTTTTATGGCACGTATTTATAAGGCTAAGA
TTATTTTGCTTAATGCAAGAATATCTGATAAATCTTATCATTCTTATCAAAGATTTTCTTTTTTTTATAAAAAAATTTTT
TCTTATATTGATGAAGTTTTTGCTCAAAGCGATTTAGATAAAGCAAGACTTGAAAGTTTGGGAGCTAAAAATGTTAAAAT
TTTTAAAAATATTAAGGCAAATTTAGAAATTAAAAATAATAAAATTTATGCCAAACCTAAGGAAAAGCTTATCATTTTTG
CTAGTACGCATAAAGATGAAGAAGAGCTTTTGCTTGATTATTTTAAACTAGAAGAAAATGAAAAACTCATTATCGCCCCA
CGACACCCTGAGCGTTTTAAAGAAGTTGAAAATTTGCTTTTAAACAAAGGTTTGGAATTTGAAAAATTTAGTTCTTTAAA
AGATGAAAATAAGAAATTTGCTAAAAAAATTTTGCTTTTAGATGCTTTAGGAGAGCTTGTAAATTTTTATACAATTTCTG
ATGTTGTTGTGCTTGGAGGTTCTTTCATAGAAGGCATAGGAGGACACAATCCTATAGAAGTGGCATATTTTGACAATGTA
TTAATCAGTGGGAAATTTATTCATAATCAAAAGGCTTTATTTGAAGAAGTAGAAAATGTATATTTTTGCGAAAATTTAAA
AGATTTAAATGATAAAATTCATTATTTAAATTTAAAAGCTAAAATTTCCAAAAAAGGAAATTTAGATCTTATCATACAGA
CCATACAAAAGGGAATTGATGCAAGAAAAAGCTTATAA

Upstream 100 bases:

>100_bases
AAATTTACGATAAGACCTATCTATCTGTTGTAAAAGGCGAAGAGATTGTAACTTGCCCCCATTGTGGAAGAATTCTTTAT
AAAGAGCAAGAAGAACAAAA

Downstream 100 bases:

>100_bases
ACTCTTAGCTTTGCAAGAAAAAATTTCAAATCGTGAAGCTAAAGATTTGATTGATAAGGGTTGTGTTTTTTCACATGGAA
AAAAAGTTGTGGTTGCTAGA

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KdtA protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MIFFYYFLTWTAFLFCAVFILLLSFLKSKYKTSLKSRFFLYKNLHQEKADVHFHACSYGEVRSIKTLVLKFDSRITTITQ
TGFECAKEFCKKVNYLAFENFLPFWFKPCKVLVIFEAEYWLMLVFMARIYKAKIILLNARISDKSYHSYQRFSFFYKKIF
SYIDEVFAQSDLDKARLESLGAKNVKIFKNIKANLEIKNNKIYAKPKEKLIIFASTHKDEEELLLDYFKLEENEKLIIAP
RHPERFKEVENLLLNKGLEFEKFSSLKDENKKFAKKILLLDALGELVNFYTISDVVVLGGSFIEGIGGHNPIEVAYFDNV
LISGKFIHNQKALFEEVENVYFCENLKDLNDKIHYLNLKAKISKKGNLDLIIQTIQKGIDARKSL

Sequences:

>Translated_385_residues
MIFFYYFLTWTAFLFCAVFILLLSFLKSKYKTSLKSRFFLYKNLHQEKADVHFHACSYGEVRSIKTLVLKFDSRITTITQ
TGFECAKEFCKKVNYLAFENFLPFWFKPCKVLVIFEAEYWLMLVFMARIYKAKIILLNARISDKSYHSYQRFSFFYKKIF
SYIDEVFAQSDLDKARLESLGAKNVKIFKNIKANLEIKNNKIYAKPKEKLIIFASTHKDEEELLLDYFKLEENEKLIIAP
RHPERFKEVENLLLNKGLEFEKFSSLKDENKKFAKKILLLDALGELVNFYTISDVVVLGGSFIEGIGGHNPIEVAYFDNV
LISGKFIHNQKALFEEVENVYFCENLKDLNDKIHYLNLKAKISKKGNLDLIIQTIQKGIDARKSL
>Mature_385_residues
MIFFYYFLTWTAFLFCAVFILLLSFLKSKYKTSLKSRFFLYKNLHQEKADVHFHACSYGEVRSIKTLVLKFDSRITTITQ
TGFECAKEFCKKVNYLAFENFLPFWFKPCKVLVIFEAEYWLMLVFMARIYKAKIILLNARISDKSYHSYQRFSFFYKKIF
SYIDEVFAQSDLDKARLESLGAKNVKIFKNIKANLEIKNNKIYAKPKEKLIIFASTHKDEEELLLDYFKLEENEKLIIAP
RHPERFKEVENLLLNKGLEFEKFSSLKDENKKFAKKILLLDALGELVNFYTISDVVVLGGSFIEGIGGHNPIEVAYFDNV
LISGKFIHNQKALFEEVENVYFCENLKDLNDKIHYLNLKAKISKKGNLDLIIQTIQKGIDARKSL

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Inner Membrane-Anchored [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=350, Percent_Identity=28, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007507
- InterPro:   IPR003358 [H]

Pfam domain/function: PF04413 Glycos_transf_N; PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 45207; Mature: 45207

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFFYYFLTWTAFLFCAVFILLLSFLKSKYKTSLKSRFFLYKNLHQEKADVHFHACSYGE
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
VRSIKTLVLKFDSRITTITQTGFECAKEFCKKVNYLAFENFLPFWFKPCKVLVIFEAEYW
HHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEECCHHH
LMLVFMARIYKAKIILLNARISDKSYHSYQRFSFFYKKIFSYIDEVFAQSDLDKARLESL
HHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
GAKNVKIFKNIKANLEIKNNKIYAKPKEKLIIFASTHKDEEELLLDYFKLEENEKLIIAP
CCCCEEEEECCEEEEEEECCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEC
RHPERFKEVENLLLNKGLEFEKFSSLKDENKKFAKKILLLDALGELVNFYTISDVVVLGG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFIEGIGGHNPIEVAYFDNVLISGKFIHNQKALFEEVENVYFCENLKDLNDKIHYLNLKA
HHHHCCCCCCCEEEEEECCEEEECEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
KISKKGNLDLIIQTIQKGIDARKSL
EECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFFYYFLTWTAFLFCAVFILLLSFLKSKYKTSLKSRFFLYKNLHQEKADVHFHACSYGE
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
VRSIKTLVLKFDSRITTITQTGFECAKEFCKKVNYLAFENFLPFWFKPCKVLVIFEAEYW
HHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEECCHHH
LMLVFMARIYKAKIILLNARISDKSYHSYQRFSFFYKKIFSYIDEVFAQSDLDKARLESL
HHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
GAKNVKIFKNIKANLEIKNNKIYAKPKEKLIIFASTHKDEEELLLDYFKLEENEKLIIAP
CCCCEEEEECCEEEEEEECCEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEC
RHPERFKEVENLLLNKGLEFEKFSSLKDENKKFAKKILLLDALGELVNFYTISDVVVLGG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFIEGIGGHNPIEVAYFDNVLISGKFIHNQKALFEEVENVYFCENLKDLNDKIHYLNLKA
HHHHCCCCCCCEEEEEECCEEEECEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
KISKKGNLDLIIQTIQKGIDARKSL
EECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]