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The map label for this gene is purA [H]

Identifier: 54293477

GI number: 54293477

Start: 572935

End: 574230

Strand: Direct

Name: purA [H]

Synonym: lpl0526

Alternate gene names: 54293477

Gene position: 572935-574230 (Clockwise)

Preceding gene: 54293476

Following gene: 54293478

Centisome position: 17.12

GC content: 41.74

Gene sequence:

>1296_bases
ATGGGTAAGAATGTTGTTGTTTTGGGTACACAATGGGGAGATGAAGGCAAAGGCAAAATTGTTGATTTGCTGACACAAGA
TGCTCAAGTGGTTGTCCGTTATCAAGGGGGCCATAATGCAGGCCATACATTAAAAATCAATGGAGTAAAAACAGTACTTC
GTTTAATTCCATCCGGGATGCTTCGCCCTAACGTAACCTGCTATGTTGCAAATGGTGTGGTTTTATCACCTCAGGCATTA
CTTAGTGAAATTAAGGAGCTTGAGGGTAATGGTATCAATGTGAGGGAGCGATTACGCATTAGCTTGGCTTGCCCCCTGAT
TCTCCCTTACCACATTGCTTTGGATAAGGCTCGTGAGACACATATGGGCAAGTCTGCAATTGGAACAACAGGTCGTGGTA
TAGGTCCTGCTTATGAGGATAAAGTGGCTCGGCGCGCTTTAAGAGTAGGCGATTTATTTCATAGAGACCGATTTGCCAAT
AAATTAACAGAATTATTGGATTATCATAATTTTGTATTAACGCAATATTTCAAACAACCAGCTGTTGATTTGGAATCCCT
GTTGGATGAGTCATTACAATGGGCTGAAGAATTAAGACCAATGGTTTGCGATGTTTCTGCCTGTTTGCATGAACATAGAA
AACAAGGTGAAAATATTTTATTTGAAGGGGCGCAAGGTGTTTATTTAGATATTGATCATGGAACATACCCCTACGTAACT
TCTTCAAATACCTGTGTGGGATCGGTAATTAATGGTGCGGGATTTGGTCCTCGCTATATTGATTACGTGTTAGGCATTAC
AAAAGCTTACACAACTCGAGTTGGCGGTGGCCCATTCCCCACTGAATTACTTGATGACGTTGGAAAACGCATTGCGGAAC
GAGGACAGGAATTTGGTGCAGTAACTGGTAGACCCAGACGTTGTGGGTGGTTTGACGCTGTTTTATTAAAACGCTCCATT
GAGTTAAATAGTATTTCAGGACTCTGTGTTACCAAATTGGATGTTCTCGATGGTTTAGAGGTATTACGGATTGCTGTGGC
TTATAAAGACAGGGATGGCAATATATTATCTCGACCTCCTTTGGCAGCCGATGATTTCAACGATTTGTTACCAGTCTATG
AGGAACTTCCTGGTTGGCAGGAATCTACTGCTGATGTGACTGTGATGAGTGACTTACCCGCCAATGCAAGGGCTTATCTA
AAGCGAATTGAAGAGATATTAGGGATTCCTATTGATATGCTATCAACAGGACCTGAAAGGGATTCAACAATTACTTTACG
AGATCCTTTTCTTTGA

Upstream 100 bases:

>100_bases
TATTTATTAGCATCATCCATCTAATATAAATGCAGTTGGGTTTCTCGTAAAATGCAACTGCATTTTTAATTTTTACAGGT
ATGCAAACAAAGAGTAAATC

Downstream 100 bases:

>100_bases
TACAAAGATAAGTTTTGTAACCACAATGAGTGTCATGCAAGAATTTCTTGATATGACACTTTCTGCCTTTTAAGTATTAT
TGTTCTGTGTTTTAGCAAGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGMLRPNVTCYVANGVVLSPQAL
LSEIKELEGNGINVRERLRISLACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSI
ELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTVMSDLPANARAYL
KRIEEILGIPIDMLSTGPERDSTITLRDPFL

Sequences:

>Translated_431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGMLRPNVTCYVANGVVLSPQAL
LSEIKELEGNGINVRERLRISLACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSI
ELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTVMSDLPANARAYL
KRIEEILGIPIDMLSTGPERDSTITLRDPFL
>Mature_430_residues
GKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGMLRPNVTCYVANGVVLSPQALL
SEIKELEGNGINVRERLRISLACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFANK
LTELLDYHNFVLTQYFKQPAVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVTS
SNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSIE
LNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTVMSDLPANARAYLK
RIEEILGIPIDMLSTGPERDSTITLRDPFL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=42.093023255814, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=40.0932400932401, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=37.844611528822, Blast_Score=258, Evalue=7e-69,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=63.953488372093, Blast_Score=571, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=41.7249417249417, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=41.7249417249417, Blast_Score=321, Evalue=4e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=42.7251732101617, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=41.9811320754717, Blast_Score=338, Evalue=6e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47484; Mature: 47353

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGM
CCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECHHHHHHHHHCCCC
LRPNVTCYVANGVVLSPQALLSEIKELEGNGINVRERLRISLACPLILPYHIALDKARET
CCCCEEEEEECCEEECHHHHHHHHHHHCCCCCCHHHHHEEEEECHHHHHHHHHHHHHHHH
HMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFANKLTELLDYHNFVLTQYFKQP
HCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEE
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGA
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPP
CCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
LAADDFNDLLPVYEELPGWQESTADVTVMSDLPANARAYLKRIEEILGIPIDMLSTGPER
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCC
DSTITLRDPFL
CCEEEECCCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGM
CCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECHHHHHHHHHCCCC
LRPNVTCYVANGVVLSPQALLSEIKELEGNGINVRERLRISLACPLILPYHIALDKARET
CCCCEEEEEECCEEECHHHHHHHHHHHCCCCCCHHHHHEEEEECHHHHHHHHHHHHHHHH
HMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFANKLTELLDYHNFVLTQYFKQP
HCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AVDLESLLDESLQWAEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEE
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGA
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPP
CCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
LAADDFNDLLPVYEELPGWQESTADVTVMSDLPANARAYLKRIEEILGIPIDMLSTGPER
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCC
DSTITLRDPFL
CCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA