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The map label for this gene is prfA

Identifier: 54020410

GI number: 54020410

Start: 267464

End: 268549

Strand: Reverse

Name: prfA

Synonym: mhp243

Alternate gene names: 54020410

Gene position: 268549-267464 (Counterclockwise)

Preceding gene: 54020411

Following gene: 54020409

Centisome position: 30.08

GC content: 34.07

Gene sequence:

>1086_bases
ATGGAAAAAAAAATGTTTAATTCTCTGATGAAAATTCATCAGAAGTATCAGGATTTAAAGCAACTTTTAGAAACAGATCA
GATCCTTAATGACCAAAAACAGTATCTACAGATTGCAAAGGAAATTGCTAGTATTACAGAAATCATTGAAGTTTTTCAAA
AATTTCTTGATGATCAAAAAGTTCTTGAAGATGCAAAAACTATTTTAATTCAGGAAGATGATCCAGAATTAATCCAACTT
GCAAAAGTAGAAATTGCAACTATGAGCAAAAACATCGAAGAATATGAAAAAAAATTGTTAATTTTAATGCTCCCAAAAGA
TAAAAATGACGAAAAGGACGTAATTGTTGAGATCCGCGGAGCGGCTGGCGGCGATGAGGCAAACATTTTTGTCGGCGATC
TTTTTAAGATGTATCATAAATGAGCTGATTCCCAAAAAGCAAAAGTAAAAGTTTTAAGCTCCTCACTTGCTTTAGCAGGC
GGGTTTAGTCAAATTATTTTTCAAATTTCAGGTCAAAAAATTTACTCAAAACTAAAATTTGAATCTGGAGTTCACCGGGT
TCAAAGAGTTCCTGCAACTGAAACAATGGGCCGAATTCATACCTCAACTGCAACAGTTACGGTTATGCCAAAAATTGATG
AAAAAATTGAGATTGAAATTAACCCTTCTGATCTAAAAATTGATACCTATCGATCTTCAGGAGCAGGGGGTCAATCAGTA
AATACAACGGATTCAGCCGTGCGGATAACCCATATTCCCACCGGTATTGTCGTAACTTCACAGGATGAAAGATCGCAAAT
TGGGAATAAAGAGATTGCAATGGGAATTCTCAAGTCCAAAATTTATAATCTTGAACTGCAAAAACAACAGCAAAAACAGT
CAGATTTTCGTAAATTAGCAGGTTCAGGAGCTCGTTCTGAAAAAATTAGAACTTATAATTATCCCCAAGATCGACTAACA
GACCATCGAATTAATTTTTCTACCTCACTAAAACCAATAATTCAAGGGAACCTAAATCCAATAATTGAAGCCTTACTTGC
CCAAGAAAAAACGGAACTAATTCTCCAAAATTATGCTAATAAATAA

Upstream 100 bases:

>100_bases
CAGCAAGGTGAATCAAATTTGATAATTTCATAGAAAAAGAAAATTCAAACATTTAGTTTTGGCTTTTAAAAAAACAAAAC
TAAATGTTTTTTAACTAATT

Downstream 100 bases:

>100_bases
GCGCAAATTAGAACTTTTAAAAGAAAAACAGCGATATAATTTACCTCTTAAGATCTCAAAATTGGAAAATTTAAAGTTAG
AACTGGACTATCCGATCCAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MEKKMFNSLMKIHQKYQDLKQLLETDQILNDQKQYLQIAKEIASITEIIEVFQKFLDDQKVLEDAKTILIQEDDPELIQL
AKVEIATMSKNIEEYEKKLLILMLPKDKNDEKDVIVEIRGAAGGDEANIFVGDLFKMYHKWADSQKAKVKVLSSSLALAG
GFSQIIFQISGQKIYSKLKFESGVHRVQRVPATETMGRIHTSTATVTVMPKIDEKIEIEINPSDLKIDTYRSSGAGGQSV
NTTDSAVRITHIPTGIVVTSQDERSQIGNKEIAMGILKSKIYNLELQKQQQKQSDFRKLAGSGARSEKIRTYNYPQDRLT
DHRINFSTSLKPIIQGNLNPIIEALLAQEKTELILQNYANK

Sequences:

>Translated_361_residues
MEKKMFNSLMKIHQKYQDLKQLLETDQILNDQKQYLQIAKEIASITEIIEVFQKFLDDQKVLEDAKTILIQEDDPELIQL
AKVEIATMSKNIEEYEKKLLILMLPKDKNDEKDVIVEIRGAAGGDEANIFVGDLFKMYHK*ADSQKAKVKVLSSSLALAG
GFSQIIFQISGQKIYSKLKFESGVHRVQRVPATETMGRIHTSTATVTVMPKIDEKIEIEINPSDLKIDTYRSSGAGGQSV
NTTDSAVRITHIPTGIVVTSQDERSQIGNKEIAMGILKSKIYNLELQKQQQKQSDFRKLAGSGARSEKIRTYNYPQDRLT
DHRINFSTSLKPIIQGNLNPIIEALLAQEKTELILQNYANK
>Mature_361_residues
MEKKMFNSLMKIHQKYQDLKQLLETDQILNDQKQYLQIAKEIASITEIIEVFQKFLDDQKVLEDAKTILIQEDDPELIQL
AKVEIATMSKNIEEYEKKLLILMLPKDKNDEKDVIVEIRGAAGGDEANIFVGDLFKMYHK*ADSQKAKVKVLSSSLALAG
GFSQIIFQISGQKIYSKLKFESGVHRVQRVPATETMGRIHTSTATVTVMPKIDEKIEIEINPSDLKIDTYRSSGAGGQSV
NTTDSAVRITHIPTGIVVTSQDERSQIGNKEIAMGILKSKIYNLELQKQQQKQSDFRKLAGSGARSEKIRTYNYPQDRLT
DHRINFSTSLKPIIQGNLNPIIEALLAQEKTELILQNYANK

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=43.2343234323432, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI34577120, Length=353, Percent_Identity=37.3937677053824, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI166795305, Length=208, Percent_Identity=41.8269230769231, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1787462, Length=354, Percent_Identity=44.9152542372881, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=34.1176470588235, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=282, Percent_Identity=38.2978723404255, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=335, Percent_Identity=38.8059701492537, Blast_Score=202, Evalue=8e-53,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=38.0664652567976, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40666; Mature: 40666

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKMFNSLMKIHQKYQDLKQLLETDQILNDQKQYLQIAKEIASITEIIEVFQKFLDDQK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VLEDAKTILIQEDDPELIQLAKVEIATMSKNIEEYEKKLLILMLPKDKNDEKDVIVEIRG
HHHHHHEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHCEEEEECCCCCCCCCCEEEEEEC
AAGGDEANIFVGDLFKMYHKADSQKAKVKVLSSSLALAGGFSQIIFQISGQKIYSKLKFE
CCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHH
SGVHRVQRVPATETMGRIHTSTATVTVMPKIDEKIEIEINPSDLKIDTYRSSGAGGQSVN
HHHHHHHHCCCHHHHHHEEECCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCC
TTDSAVRITHIPTGIVVTSQDERSQIGNKEIAMGILKSKIYNLELQKQQQKQSDFRKLAG
CCCCEEEEEECCCCEEEECCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
SGARSEKIRTYNYPQDRLTDHRINFSTSLKPIIQGNLNPIIEALLAQEKTELILQNYANK
CCCCCCCCEECCCCHHHCCCCEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKKMFNSLMKIHQKYQDLKQLLETDQILNDQKQYLQIAKEIASITEIIEVFQKFLDDQK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VLEDAKTILIQEDDPELIQLAKVEIATMSKNIEEYEKKLLILMLPKDKNDEKDVIVEIRG
HHHHHHEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHCEEEEECCCCCCCCCCEEEEEEC
AAGGDEANIFVGDLFKMYHKADSQKAKVKVLSSSLALAGGFSQIIFQISGQKIYSKLKFE
CCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHH
SGVHRVQRVPATETMGRIHTSTATVTVMPKIDEKIEIEINPSDLKIDTYRSSGAGGQSVN
HHHHHHHHCCCHHHHHHEEECCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCC
TTDSAVRITHIPTGIVVTSQDERSQIGNKEIAMGILKSKIYNLELQKQQQKQSDFRKLAG
CCCCEEEEEECCCCEEEECCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
SGARSEKIRTYNYPQDRLTDHRINFSTSLKPIIQGNLNPIIEALLAQEKTELILQNYANK
CCCCCCCCEECCCCHHHCCCCEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA