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The map label for this gene is oppF

Identifier: 54020375

GI number: 54020375

Start: 201254

End: 202585

Strand: Reverse

Name: oppF

Synonym: mhp166

Alternate gene names: 54020375

Gene position: 202585-201254 (Counterclockwise)

Preceding gene: 54020376

Following gene: 54020374

Centisome position: 22.69

GC content: 29.13

Gene sequence:

>1332_bases
ATGGAAGATAAAAAAAGATTTTACCTAGAAAAAAATAGTTATTTTTTTGGCAAAATAGTAAAGAATATTTACCGCGCACT
TACTCCCGGTATTGAAAAAATGCTTGAATTTAAAGGCAAATCACCAATTGTCAGCTTTCGAAATGTTGATATTACCTATG
GAAGTGGTTCAAGAAAAAATAAAGTAATTCATGATATTAGTTTTAATATTTATGAAGGTGAAGTTCTATCTTTTGTTGGC
GAATCTGGTTCTGGAAAATCAACAACAGGTTCAGCACTTGCGGGTTTAGTTCCCCGTAGTTTTGGCCAAATTGTAATTAA
CGGATTTGAGCTACCAAAAAATATCCGGAAAATAAAGGCAAAAATTCTTGGAAAACTAGTCTCAAATGTGCAAATGATTT
TCCAAGATCCATTATCTTCATTAAATCCTTATAAAAATATTTATGATGTAATTACAGAAGGTCTTGTAAATTTAGAACAA
CGCCAAAAAGGTTCAACGAAAATTTTATTTTCACAACATTATTACCAAAATACATTTGAATCTTTAGTTTCTGCCCTTAA
AAAGCAAAAAATTTCTCCAGAAATTATCCAGCAAATTAATAATAATTTCTATAAAAATACAAAGGATATTCCTGTTGAAA
ATTTGTTTAAAACTCTAATAAATTACCTTGAAAAACTAAGAGTTATCGAGACTAAAATTATTGACTATATTCAAAAAAAG
TCTTTATTTTTACAGGATTTTTTAAAAAAACCACTAAAAGATATTACCTATAAATATGTTATTGACATGTTAGCCTCTGT
TGGACTTGATAGCTCAGTTTTATCCCGTTATCCACTTGAATTCTCAGGGGGCCAACAACAAAGAATTGGAATTTGCCGTG
CTTTATTATTAAGGCCGAAAATTCTTGTTGCCGATGAGCCAATTTCAGCACTTGATGTCTCAATTCAAGCACAAATTATT
AATATTTTTAAGGATTTAAAAGAAAAATATAACCTGACAATCTTTTTTATTTCCCATGATCTACGAATGGTTGAATATAT
CTCTGATCGAATCGCGGTAATTTATCGCGGAAGAATTTTAGAAATTGGACCTTCAAAGGAGATAATTAAAAATTTTTTGC
ATCCTTATACAAAATCATTAATTGAATCAATTCCAACAATTGAATCAAAACGAGGATCGCTTGCTGGTTATGTCTATGAT
CCAAAAATACATCAATATGATGAAAAAAATCAACCTCAATGAATTAATTTAGGAAATAATCACTATATTTTAGCAACTTT
TGCCGAACTAAAAAGGTGAAAATTAGGAGACTATAATTATGAAAAAATCTAA

Upstream 100 bases:

>100_bases
CGAGTTAAATCAGCTTTACTTGATCCAAAAGCACCAAAAGTTGTCCCACCAAAAATTATTTACCAAAAATGACTTCAATT
TGCAAAGATGAGGCAAGAAA

Downstream 100 bases:

>100_bases
TATTTTGCTTAGTTATTTATTAGCAGGCGGAATTCTTGCTGGGGCTTCAGCCGGAATTATGGGTGGAATTAAAATCTATT
CAGAAAATAATGAACTAGGT

Product: oligopeptide ABC transporter ATP-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KE [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MEDKKRFYLEKNSYFFGKIVKNIYRALTPGIEKMLEFKGKSPIVSFRNVDITYGSGSRKNKVIHDISFNIYEGEVLSFVG
ESGSGKSTTGSALAGLVPRSFGQIVINGFELPKNIRKIKAKILGKLVSNVQMIFQDPLSSLNPYKNIYDVITEGLVNLEQ
RQKGSTKILFSQHYYQNTFESLVSALKKQKISPEIIQQINNNFYKNTKDIPVENLFKTLINYLEKLRVIETKIIDYIQKK
SLFLQDFLKKPLKDITYKYVIDMLASVGLDSSVLSRYPLEFSGGQQQRIGICRALLLRPKILVADEPISALDVSIQAQII
NIFKDLKEKYNLTIFFISHDLRMVEYISDRIAVIYRGRILEIGPSKEIIKNFLHPYTKSLIESIPTIESKRGSLAGYVYD
PKIHQYDEKNQPQWINLGNNHYILATFAELKRWKLGDYNYEKI

Sequences:

>Translated_443_residues
MEDKKRFYLEKNSYFFGKIVKNIYRALTPGIEKMLEFKGKSPIVSFRNVDITYGSGSRKNKVIHDISFNIYEGEVLSFVG
ESGSGKSTTGSALAGLVPRSFGQIVINGFELPKNIRKIKAKILGKLVSNVQMIFQDPLSSLNPYKNIYDVITEGLVNLEQ
RQKGSTKILFSQHYYQNTFESLVSALKKQKISPEIIQQINNNFYKNTKDIPVENLFKTLINYLEKLRVIETKIIDYIQKK
SLFLQDFLKKPLKDITYKYVIDMLASVGLDSSVLSRYPLEFSGGQQQRIGICRALLLRPKILVADEPISALDVSIQAQII
NIFKDLKEKYNLTIFFISHDLRMVEYISDRIAVIYRGRILEIGPSKEIIKNFLHPYTKSLIESIPTIESKRGSLAGYVYD
PKIHQYDEKNQPQ*INLGNNHYILATFAELKR*KLGDYNYEKI
>Mature_443_residues
MEDKKRFYLEKNSYFFGKIVKNIYRALTPGIEKMLEFKGKSPIVSFRNVDITYGSGSRKNKVIHDISFNIYEGEVLSFVG
ESGSGKSTTGSALAGLVPRSFGQIVINGFELPKNIRKIKAKILGKLVSNVQMIFQDPLSSLNPYKNIYDVITEGLVNLEQ
RQKGSTKILFSQHYYQNTFESLVSALKKQKISPEIIQQINNNFYKNTKDIPVENLFKTLINYLEKLRVIETKIIDYIQKK
SLFLQDFLKKPLKDITYKYVIDMLASVGLDSSVLSRYPLEFSGGQQQRIGICRALLLRPKILVADEPISALDVSIQAQII
NIFKDLKEKYNLTIFFISHDLRMVEYISDRIAVIYRGRILEIGPSKEIIKNFLHPYTKSLIESIPTIESKRGSLAGYVYD
PKIHQYDEKNQPQ*INLGNNHYILATFAELKR*KLGDYNYEKI

Specific function: Component of the oligopeptide permease, a binding protein-dependent transport system. Necessary for genetic competence but not sporulation. Probably responsible for energy coupling to the transport system [H]

COG id: COG1124

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1787500, Length=123, Percent_Identity=52.0325203252033, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI145693107, Length=141, Percent_Identity=50.354609929078, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789962, Length=146, Percent_Identity=44.5205479452055, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787758, Length=127, Percent_Identity=44.8818897637795, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994883, Length=114, Percent_Identity=44.7368421052632, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1788506, Length=124, Percent_Identity=41.9354838709677, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789963, Length=168, Percent_Identity=38.0952380952381, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787547, Length=124, Percent_Identity=37.0967741935484, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787759, Length=176, Percent_Identity=31.25, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787548, Length=134, Percent_Identity=37.3134328358209, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1789891, Length=125, Percent_Identity=36.8, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI48994997, Length=107, Percent_Identity=37.3831775700935, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789032, Length=125, Percent_Identity=39.2, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787029, Length=119, Percent_Identity=38.655462184874, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786398, Length=125, Percent_Identity=40, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788644, Length=162, Percent_Identity=29.0123456790123, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788225, Length=121, Percent_Identity=35.5371900826446, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787089, Length=105, Percent_Identity=40.9523809523809, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789890, Length=138, Percent_Identity=34.0579710144928, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787370, Length=120, Percent_Identity=40, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788761, Length=136, Percent_Identity=27.9411764705882, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787712, Length=109, Percent_Identity=33.9449541284404, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081709, Length=112, Percent_Identity=33.0357142857143, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081791, Length=119, Percent_Identity=34.453781512605, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789672, Length=137, Percent_Identity=29.1970802919708, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788450, Length=119, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787164, Length=93, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=105, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786253, Length=105, Percent_Identity=31.4285714285714, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790162, Length=98, Percent_Identity=34.6938775510204, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790467, Length=108, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786563, Length=101, Percent_Identity=34.6534653465347, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789864, Length=157, Percent_Identity=28.0254777070064, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789873, Length=83, Percent_Identity=32.5301204819277, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17565938, Length=93, Percent_Identity=39.7849462365591, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115534520, Length=99, Percent_Identity=36.3636363636364, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324985, Length=91, Percent_Identity=39.5604395604396, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 50613; Mature: 50613

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKKRFYLEKNSYFFGKIVKNIYRALTPGIEKMLEFKGKSPIVSFRNVDITYGSGSRKN
CCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCC
KVIHDISFNIYEGEVLSFVGESGSGKSTTGSALAGLVPRSFGQIVINGFELPKNIRKIKA
CEEEEEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHEEECHHCHHHHHHHHH
KILGKLVSNVQMIFQDPLSSLNPYKNIYDVITEGLVNLEQRQKGSTKILFSQHYYQNTFE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
SLVSALKKQKISPEIIQQINNNFYKNTKDIPVENLFKTLINYLEKLRVIETKIIDYIQKK
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFLQDFLKKPLKDITYKYVIDMLASVGLDSSVLSRYPLEFSGGQQQRIGICRALLLRPK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
ILVADEPISALDVSIQAQIINIFKDLKEKYNLTIFFISHDLRMVEYISDRIAVIYRGRIL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHEEEECCEEE
EIGPSKEIIKNFLHPYTKSLIESIPTIESKRGSLAGYVYDPKIHQYDEKNQPQINLGNNH
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCC
YILATFAELKRKLGDYNYEKI
EEEEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEDKKRFYLEKNSYFFGKIVKNIYRALTPGIEKMLEFKGKSPIVSFRNVDITYGSGSRKN
CCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCC
KVIHDISFNIYEGEVLSFVGESGSGKSTTGSALAGLVPRSFGQIVINGFELPKNIRKIKA
CEEEEEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHEEECHHCHHHHHHHHH
KILGKLVSNVQMIFQDPLSSLNPYKNIYDVITEGLVNLEQRQKGSTKILFSQHYYQNTFE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
SLVSALKKQKISPEIIQQINNNFYKNTKDIPVENLFKTLINYLEKLRVIETKIIDYIQKK
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLFLQDFLKKPLKDITYKYVIDMLASVGLDSSVLSRYPLEFSGGQQQRIGICRALLLRPK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
ILVADEPISALDVSIQAQIINIFKDLKEKYNLTIFFISHDLRMVEYISDRIAVIYRGRIL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHEEEECCEEE
EIGPSKEIIKNFLHPYTKSLIESIPTIESKRGSLAGYVYDPKIHQYDEKNQPQINLGNNH
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCC
YILATFAELKRKLGDYNYEKI
EEEEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]