Click here to switch to the map view.

The map label for this gene is iolD

Identifier: 54020367

GI number: 54020367

Start: 178213

End: 180183

Strand: Reverse

Name: iolD

Synonym: mhp149

Alternate gene names: 54020367

Gene position: 180183-178213 (Counterclockwise)

Preceding gene: 54020368

Following gene: 54020366

Centisome position: 20.18

GC content: 34.45

Gene sequence:

>1971_bases
ATGGCAAAGACAATTCGACTAACAACCGCACAGGCAATTATTAAATTTATTGATAATGTTTATCTAAAAATTGATGCAAA
AACAACCAAATTTGTCTATGGAATAGCTACTATTTTCGGTCATGGAAACGTTTTAGGATTAGGCGAAGCGCTTGCAAATA
TTGACCATAGTCTAAAATTAATTCAGGGCAAAAATGAACAAGGAATGGCTCATCTTGCCCTTGGTTTTACAAAGCAAAAA
CATCGCAGACAAATTATTGCTTGTACTTCCTCAGTTGGTCCTGGAGCTGCAAATATGTTAACAGCAGCCGGAACTGCAAC
TGCTAATAATCTTCCATTATTACTTTTTCCAGGCGATACTTTTGCCTCAAGAAGACCCGATCCTGTGCTTCAACAAATCG
AACAAATCCATGATTTAAACATCACTACAAATGATGCTTTTAAACCTTTTTCGAAATTCTGAGATCGAATTAATCGTCCT
GAACAAATTATTAATTCACTAATTCATGCTTTTGATATTCTCACTGATCCTGCAAATACCGGAGCAGTAACAATTTGCCT
TCCACAAGATGTTCAAGCTGAAGTCTATGATTTCCCTGAATGATTTTTTGAGAAAAGAGTCTATGAAATCAAAAGGATAA
AGGCTGATCGCAAAGATATAGAAAAATTAGTTGATCTAATCAAAAATGCCCAGCAACCTTTGGTGATCATCGGTGGCGGT
ATTCGTTATTCCCAGGCAAGTAGTCAACTTCTTGAATTTCTAAGAAAAACAAAAATTCCTTTTAGTTTTACCCAGGCCGG
AAAAAGTTCAATTCCTTCATCTTTAAAGCAAAATTTAGGCGGAATTGGTGTAACAGGAACTCTTATTGCTAATTTATATG
CTAAAAAAGCCGATTTAATCATTGGTCTAGGAACAAAATATTCTGATTTTACAACCGGCTCAAAAACCCAATTTGCTAAA
GAAGCCAAATTTGTTAATATTAATATTAAATCTTTTGATGCCCGGAAAATGAATGGACTAGCAATTATTGCCGATCTTAA
AGTTGCCCTTGAAAGTCTAAATTACTTACTTAAAAATACACAAGAATTTGGATATATTAAAAAGATCAAAGAAAATAAAG
AACTTTTAAAGGCAAAAGACCAATGAGAAGACTATCTTAAAAAGATCTATGAACTTGAAAAACCACTTGAAAACAAAGCG
CTTGTCCTTGATAAAAATCAAAACTCGCCAGAAAAATTCGCCTTAGCAATTAAGGAGCATTACCAAAAAGATATTCACAT
TTTTAGCCAAAGTCGCGCTTTGTATCTAATCAGGAAATATATTGATCCTAAAGCTTCAATTACCGCTGCAGCGGGTTCAC
TTCCTGGGGATCTCCAAAGACTTTGAGAGACAGATGAATTTGGATCTTATAATGTTGAATATGGCTATTCTTGCATGGGA
CATGAGATTCAAGCAGCAATTGGTTCATCAATTGCCCTTGGTTTTAGACCAACTTATGCCCTTGTTGGCGATGGATCTTT
TCTGATGCTTCATAGCGAAATGCTTACGGCAATTCAGGAACAAGTTCCAGTTGTGATTATCCTCTTTGATAATTCCGGTT
TTGGATGTATTAATAATTTGCAGGTTGGATCAAATATTAGATCTTTTGAGACTGAATTTTATACAAGATCACAGAAAAAT
CTAAATGAATATGGTTCTCTAGTGATTACCGAATTTGCCACAATTGCAAAAGGTTATGGATTTAGATCTGTTTTTGCCAG
GTCAGAACAGGAATTTATTATGGCCCTAAAACAGTCAAAAAGAACGAAAAAACCAATGTTAATTGAGATCAAAGTTTATC
CTAAATCAATGACTCCTGGCTTTGAGTCTTTCTGACAAACCGGCCTTTCACTTGTATCTAAAAATAAAGAAATTGAAGAA
CTTGCCAAAGAAAATGAAAAAATCCTTAAGGAGAATAACAATTTTAACTAA

Upstream 100 bases:

>100_bases
TAACAATTGATGATGGTTATAAAGCGGTTTTACTTGCAAAAGCTGCAAAAATATCTCTAAAAGAAAAACGATTAGTGAAA
ATAAGTGAGTTAGAGGAATA

Downstream 100 bases:

>100_bases
TGAATAAAATCTGAAAACTAAAAAATGTTAAAGTCGGTGTTGCTCCAATTTTATGAACAAATGATGATATGCCTGAATTA
GGAGGAGATATTTCCTTTGA

Product: myo-inositol catabolism protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MAKTIRLTTAQAIIKFIDNVYLKIDAKTTKFVYGIATIFGHGNVLGLGEALANIDHSLKLIQGKNEQGMAHLALGFTKQK
HRRQIIACTSSVGPGAANMLTAAGTATANNLPLLLFPGDTFASRRPDPVLQQIEQIHDLNITTNDAFKPFSKFWDRINRP
EQIINSLIHAFDILTDPANTGAVTICLPQDVQAEVYDFPEWFFEKRVYEIKRIKADRKDIEKLVDLIKNAQQPLVIIGGG
IRYSQASSQLLEFLRKTKIPFSFTQAGKSSIPSSLKQNLGGIGVTGTLIANLYAKKADLIIGLGTKYSDFTTGSKTQFAK
EAKFVNINIKSFDARKMNGLAIIADLKVALESLNYLLKNTQEFGYIKKIKENKELLKAKDQWEDYLKKIYELEKPLENKA
LVLDKNQNSPEKFALAIKEHYQKDIHIFSQSRALYLIRKYIDPKASITAAAGSLPGDLQRLWETDEFGSYNVEYGYSCMG
HEIQAAIGSSIALGFRPTYALVGDGSFLMLHSEMLTAIQEQVPVVIILFDNSGFGCINNLQVGSNIRSFETEFYTRSQKN
LNEYGSLVITEFATIAKGYGFRSVFARSEQEFIMALKQSKRTKKPMLIEIKVYPKSMTPGFESFWQTGLSLVSKNKEIEE
LAKENEKILKENNNFN

Sequences:

>Translated_656_residues
MAKTIRLTTAQAIIKFIDNVYLKIDAKTTKFVYGIATIFGHGNVLGLGEALANIDHSLKLIQGKNEQGMAHLALGFTKQK
HRRQIIACTSSVGPGAANMLTAAGTATANNLPLLLFPGDTFASRRPDPVLQQIEQIHDLNITTNDAFKPFSKF*DRINRP
EQIINSLIHAFDILTDPANTGAVTICLPQDVQAEVYDFPE*FFEKRVYEIKRIKADRKDIEKLVDLIKNAQQPLVIIGGG
IRYSQASSQLLEFLRKTKIPFSFTQAGKSSIPSSLKQNLGGIGVTGTLIANLYAKKADLIIGLGTKYSDFTTGSKTQFAK
EAKFVNINIKSFDARKMNGLAIIADLKVALESLNYLLKNTQEFGYIKKIKENKELLKAKDQ*EDYLKKIYELEKPLENKA
LVLDKNQNSPEKFALAIKEHYQKDIHIFSQSRALYLIRKYIDPKASITAAAGSLPGDLQRL*ETDEFGSYNVEYGYSCMG
HEIQAAIGSSIALGFRPTYALVGDGSFLMLHSEMLTAIQEQVPVVIILFDNSGFGCINNLQVGSNIRSFETEFYTRSQKN
LNEYGSLVITEFATIAKGYGFRSVFARSEQEFIMALKQSKRTKKPMLIEIKVYPKSMTPGFESF*QTGLSLVSKNKEIEE
LAKENEKILKENNNFN
>Mature_655_residues
AKTIRLTTAQAIIKFIDNVYLKIDAKTTKFVYGIATIFGHGNVLGLGEALANIDHSLKLIQGKNEQGMAHLALGFTKQKH
RRQIIACTSSVGPGAANMLTAAGTATANNLPLLLFPGDTFASRRPDPVLQQIEQIHDLNITTNDAFKPFSKF*DRINRPE
QIINSLIHAFDILTDPANTGAVTICLPQDVQAEVYDFPE*FFEKRVYEIKRIKADRKDIEKLVDLIKNAQQPLVIIGGGI
RYSQASSQLLEFLRKTKIPFSFTQAGKSSIPSSLKQNLGGIGVTGTLIANLYAKKADLIIGLGTKYSDFTTGSKTQFAKE
AKFVNINIKSFDARKMNGLAIIADLKVALESLNYLLKNTQEFGYIKKIKENKELLKAKDQ*EDYLKKIYELEKPLENKAL
VLDKNQNSPEKFALAIKEHYQKDIHIFSQSRALYLIRKYIDPKASITAAAGSLPGDLQRL*ETDEFGSYNVEYGYSCMGH
EIQAAIGSSIALGFRPTYALVGDGSFLMLHSEMLTAIQEQVPVVIILFDNSGFGCINNLQVGSNIRSFETEFYTRSQKNL
NEYGSLVITEFATIAKGYGFRSVFARSEQEFIMALKQSKRTKKPMLIEIKVYPKSMTPGFESF*QTGLSLVSKNKEIEEL
AKENEKILKENNNFN

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=620, Percent_Identity=24.3548387096774, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1786717, Length=568, Percent_Identity=22.3591549295775, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI87081685, Length=516, Percent_Identity=23.8372093023256, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=22.5524475524476, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788716, Length=541, Percent_Identity=21.6266173752311, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=511, Percent_Identity=22.7005870841487, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531301, Length=511, Percent_Identity=22.7005870841487, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=294, Percent_Identity=26.8707482993197, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922626, Length=621, Percent_Identity=21.0950080515298, Blast_Score=83, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 72500; Mature: 72369

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIRLTTAQAIIKFIDNVYLKIDAKTTKFVYGIATIFGHGNVLGLGEALANIDHSLKL
CCCEEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHCCCHHEEE
IQGKNEQGMAHLALGFTKQKHRRQIIACTSSVGPGAANMLTAAGTATANNLPLLLFPGDT
EECCCCCCCEEEEECCHHHHHCCEEEEEECCCCCCHHHHHEECCCCCCCCCEEEEECCCC
FASRRPDPVLQQIEQIHDLNITTNDAFKPFSKFDRINRPEQIINSLIHAFDILTDPANTG
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQAEVYDFPEFFEKRVYEIKRIKADRKDIEKLVDLIKNAQQPLVIIGGGIR
EEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCC
YSQASSQLLEFLRKTKIPFSFTQAGKSSIPSSLKQNLGGIGVTGTLIANLYAKKADLIIG
CHHHHHHHHHHHHHCCCCCCHHHCCHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
LGTKYSDFTTGSKTQFAKEAKFVNINIKSFDARKMNGLAIIADLKVALESLNYLLKNTQE
CCCCCCCCCCCCCHHHHHCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHH
FGYIKKIKENKELLKAKDQEDYLKKIYELEKPLENKALVLDKNQNSPEKFALAIKEHYQK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
DIHIFSQSRALYLIRKYIDPKASITAAAGSLPGDLQRLETDEFGSYNVEYGYSCMGHEIQ
HHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHH
AAIGSSIALGFRPTYALVGDGSFLMLHSEMLTAIQEQVPVVIILFDNSGFGCINNLQVGS
HHHCCCEEECCCCCEEEEECCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCC
NIRSFETEFYTRSQKNLNEYGSLVITEFATIAKGYGFRSVFARSEQEFIMALKQSKRTKK
CCHHHHHHHHHCCHHCHHHHHCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCC
PMLIEIKVYPKSMTPGFESFQTGLSLVSKNKEIEELAKENEKILKENNNFN
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AKTIRLTTAQAIIKFIDNVYLKIDAKTTKFVYGIATIFGHGNVLGLGEALANIDHSLKL
CCEEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEEHHHHHHCCCHHEEE
IQGKNEQGMAHLALGFTKQKHRRQIIACTSSVGPGAANMLTAAGTATANNLPLLLFPGDT
EECCCCCCCEEEEECCHHHHHCCEEEEEECCCCCCHHHHHEECCCCCCCCCEEEEECCCC
FASRRPDPVLQQIEQIHDLNITTNDAFKPFSKFDRINRPEQIINSLIHAFDILTDPANTG
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQAEVYDFPEFFEKRVYEIKRIKADRKDIEKLVDLIKNAQQPLVIIGGGIR
EEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCC
YSQASSQLLEFLRKTKIPFSFTQAGKSSIPSSLKQNLGGIGVTGTLIANLYAKKADLIIG
CHHHHHHHHHHHHHCCCCCCHHHCCHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCEEEE
LGTKYSDFTTGSKTQFAKEAKFVNINIKSFDARKMNGLAIIADLKVALESLNYLLKNTQE
CCCCCCCCCCCCCHHHHHCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCHH
FGYIKKIKENKELLKAKDQEDYLKKIYELEKPLENKALVLDKNQNSPEKFALAIKEHYQK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
DIHIFSQSRALYLIRKYIDPKASITAAAGSLPGDLQRLETDEFGSYNVEYGYSCMGHEIQ
HHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHH
AAIGSSIALGFRPTYALVGDGSFLMLHSEMLTAIQEQVPVVIILFDNSGFGCINNLQVGS
HHHCCCEEECCCCCEEEEECCCEEEEHHHHHHHHHHCCCEEEEEECCCCCCEECCCCCCC
NIRSFETEFYTRSQKNLNEYGSLVITEFATIAKGYGFRSVFARSEQEFIMALKQSKRTKK
CCHHHHHHHHHCCHHCHHHHHCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCCC
PMLIEIKVYPKSMTPGFESFQTGLSLVSKNKEIEELAKENEKILKENNNFN
CEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA