Click here to switch to the map view.

The map label for this gene is hisS

Identifier: 54020360

GI number: 54020360

Start: 163685

End: 164971

Strand: Reverse

Name: hisS

Synonym: mhp138

Alternate gene names: 54020360

Gene position: 164971-163685 (Counterclockwise)

Preceding gene: 54020361

Following gene: 54020359

Centisome position: 18.48

GC content: 26.03

Gene sequence:

>1287_bases
GTGAAAAAAAAGTTAAATTTATGCCCAAAAGGAACCTATGATTTCTTTGGCCAAGGTGCTAAAATTTTCATTGATGTTCG
TAAAGTTTTTTTTAATCAGGCAAAAAAATTTAATTTTTCTTATATTGAAACACCAATTTTTGAATATGCAAATATTTTTC
TAACAACAAATCAAATCGCAGATATTGTAAGTAAAGAATTATATAAATTTTTTGATAAATCAGGACGTGAATTGGCACTA
AGACCTGAAGGTACAGCCCCAATTATGCGCTCGGTAGCCCAGCATAAATTATTCCAAACAGAAAAGAAATTTTTCTATTT
TGGCCCAATGTTTCGCTATGAAAACCCTCAAAAAGGTCGATTTCGTCAGTTTTATCAAGCCGGATTTGAAATTATTAATT
ATAAAAAAGATTCACTGGAATTCCAAATTCTTGAAATTATTCTTTTAATTAAATCGATTTTTAAAGACCTTGGGATAAAT
GAATATGAAATTAAAATTAATTTCCTTTCAAATCTAACAACAAGGAATATTTATAAAAAAAATTTGGCCCAATATTTTGA
AAAATTCAGTGATAAACTTGAGCCAATTTCAAAAATACGGATTAAGAAAAATCCACTACGGATTCTTGATGATAAAATTG
AGCAGGAAAAAGAATTTGTCAAATTAGCTCCTAAAATAAATACTTTTTGAACAATGGAAGATAAAAATATTTTTAATAGA
ATAACTTCAATTCTGGAAGAATTTAAAATCATCTATAAAGTTGATTATAATTTAGTCCGTGGTCTTGATTATTATGATGA
TTTTGTTTTTGAATTTATTGATACTTCACAAACACTTGGGACAAAATTAGCGCTTGTAGGAGGAGGTTGTTATAATAATT
TGCCGACAAAATTTGGGCTTAATAATTTTAAAAGTATTGGAATGGCCTTTGGAATTGAACGCTTAATTGAGATTATAAAA
TCAAAAAAGAACATAAAAGAGCAAAATCTTGACTTTTTTTTATTAAGCTTTACCGATAAAGAAATTTTGCTAAATTTTAA
ATTAGCAAAAATATTACGAAAAGAAAATTTTTTAGTCGATTTAAATAAAACACCATTTTCAGTAAGTAAAGGTTTTCAAT
TAGCCAAAAAATCTGGAGCTAAATTTGTTTTTTTCTTTGAAAAAAATCAAGCAAAAAATTATATTTCTCTAAAAAATTTG
CAAACTGGAAAGAATGAACAAATTTTATATACTGAAATTAATTTTGAATATCTAAATTCAATTATCAAAGCGAGTGAAAA
TGCATAA

Upstream 100 bases:

>100_bases
TATGAAAAATAATATAAATCAACTTTTTCTATTGTGGGGAAAATCAAAAATGTTGATTTTTTTCTAATATTTTATTTGAT
AACTTAAAAAAAGGTAAAAA

Downstream 100 bases:

>100_bases
TTCGAAAACTTTATCCAAATCTGATCTCGGAAAAACAGTTACAATTCAAGGTTGAGTACAAAATATTAGGAAAATTAAAG
GCAAAAGTTTCGTGATAATC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKKKLNLCPKGTYDFFGQGAKIFIDVRKVFFNQAKKFNFSYIETPIFEYANIFLTTNQIADIVSKELYKFFDKSGRELAL
RPEGTAPIMRSVAQHKLFQTEKKFFYFGPMFRYENPQKGRFRQFYQAGFEIINYKKDSLEFQILEIILLIKSIFKDLGIN
EYEIKINFLSNLTTRNIYKKNLAQYFEKFSDKLEPISKIRIKKNPLRILDDKIEQEKEFVKLAPKINTFWTMEDKNIFNR
ITSILEEFKIIYKVDYNLVRGLDYYDDFVFEFIDTSQTLGTKLALVGGGCYNNLPTKFGLNNFKSIGMAFGIERLIEIIK
SKKNIKEQNLDFFLLSFTDKEILLNFKLAKILRKENFLVDLNKTPFSVSKGFQLAKKSGAKFVFFFEKNQAKNYISLKNL
QTGKNEQILYTEINFEYLNSIIKASENA

Sequences:

>Translated_428_residues
MKKKLNLCPKGTYDFFGQGAKIFIDVRKVFFNQAKKFNFSYIETPIFEYANIFLTTNQIADIVSKELYKFFDKSGRELAL
RPEGTAPIMRSVAQHKLFQTEKKFFYFGPMFRYENPQKGRFRQFYQAGFEIINYKKDSLEFQILEIILLIKSIFKDLGIN
EYEIKINFLSNLTTRNIYKKNLAQYFEKFSDKLEPISKIRIKKNPLRILDDKIEQEKEFVKLAPKINTF*TMEDKNIFNR
ITSILEEFKIIYKVDYNLVRGLDYYDDFVFEFIDTSQTLGTKLALVGGGCYNNLPTKFGLNNFKSIGMAFGIERLIEIIK
SKKNIKEQNLDFFLLSFTDKEILLNFKLAKILRKENFLVDLNKTPFSVSKGFQLAKKSGAKFVFFFEKNQAKNYISLKNL
QTGKNEQILYTEINFEYLNSIIKASENA
>Mature_428_residues
MKKKLNLCPKGTYDFFGQGAKIFIDVRKVFFNQAKKFNFSYIETPIFEYANIFLTTNQIADIVSKELYKFFDKSGRELAL
RPEGTAPIMRSVAQHKLFQTEKKFFYFGPMFRYENPQKGRFRQFYQAGFEIINYKKDSLEFQILEIILLIKSIFKDLGIN
EYEIKINFLSNLTTRNIYKKNLAQYFEKFSDKLEPISKIRIKKNPLRILDDKIEQEKEFVKLAPKINTF*TMEDKNIFNR
ITSILEEFKIIYKVDYNLVRGLDYYDDFVFEFIDTSQTLGTKLALVGGGCYNNLPTKFGLNNFKSIGMAFGIERLIEIIK
SKKNIKEQNLDFFLLSFTDKEILLNFKLAKILRKENFLVDLNKTPFSVSKGFQLAKKSGAKFVFFFEKNQAKNYISLKNL
QTGKNEQILYTEINFEYLNSIIKASENA

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=407, Percent_Identity=30.958230958231, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71993693, Length=448, Percent_Identity=20.3125, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993686, Length=448, Percent_Identity=20.3125, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24643061, Length=359, Percent_Identity=26.4623955431755, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50197; Mature: 50197

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLNLCPKGTYDFFGQGAKIFIDVRKVFFNQAKKFNFSYIETPIFEYANIFLTTNQIA
CCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHCCCCHHHHCHHHHHHHEEEEHHHHH
DIVSKELYKFFDKSGRELALRPEGTAPIMRSVAQHKLFQTEKKFFYFGPMFRYENPQKGR
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCCHH
FRQFYQAGFEIINYKKDSLEFQILEIILLIKSIFKDLGINEYEIKINFLSNLTTRNIYKK
HHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHHHH
NLAQYFEKFSDKLEPISKIRIKKNPLRILDDKIEQEKEFVKLAPKINTFTMEDKNIFNRI
HHHHHHHHHHHHHCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHH
TSILEEFKIIYKVDYNLVRGLDYYDDFVFEFIDTSQTLGTKLALVGGGCYNNLPTKFGLN
HHHHHHHHHHHEECHHHHHCCCHHHHHHHHHHHCCHHHCCEEEEEECCCCCCCCCCCCCC
NFKSIGMAFGIERLIEIIKSKKNIKEQNLDFFLLSFTDKEILLNFKLAKILRKENFLVDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEE
NKTPFSVSKGFQLAKKSGAKFVFFFEKNQAKNYISLKNLQTGKNEQILYTEINFEYLNSI
CCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHH
IKASENA
HHCCCCC
>Mature Secondary Structure
MKKKLNLCPKGTYDFFGQGAKIFIDVRKVFFNQAKKFNFSYIETPIFEYANIFLTTNQIA
CCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHCCCCHHHHCHHHHHHHEEEEHHHHH
DIVSKELYKFFDKSGRELALRPEGTAPIMRSVAQHKLFQTEKKFFYFGPMFRYENPQKGR
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCCHH
FRQFYQAGFEIINYKKDSLEFQILEIILLIKSIFKDLGINEYEIKINFLSNLTTRNIYKK
HHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHHHH
NLAQYFEKFSDKLEPISKIRIKKNPLRILDDKIEQEKEFVKLAPKINTFTMEDKNIFNRI
HHHHHHHHHHHHHCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHH
TSILEEFKIIYKVDYNLVRGLDYYDDFVFEFIDTSQTLGTKLALVGGGCYNNLPTKFGLN
HHHHHHHHHHHEECHHHHHCCCHHHHHHHHHHHCCHHHCCEEEEEECCCCCCCCCCCCCC
NFKSIGMAFGIERLIEIIKSKKNIKEQNLDFFLLSFTDKEILLNFKLAKILRKENFLVDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEE
NKTPFSVSKGFQLAKKSGAKFVFFFEKNQAKNYISLKNLQTGKNEQILYTEINFEYLNSI
CCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCCCCCCEEEEEECCHHHHHHH
IKASENA
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA