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The map label for this gene is aspS

Identifier: 54020359

GI number: 54020359

Start: 161989

End: 163698

Strand: Reverse

Name: aspS

Synonym: mhp137

Alternate gene names: 54020359

Gene position: 163698-161989 (Counterclockwise)

Preceding gene: 54020360

Following gene: 54020358

Centisome position: 18.34

GC content: 32.22

Gene sequence:

>1710_bases
GTGAAAATGCATAATTCGAAAACTTTATCCAAATCTGATCTCGGAAAAACAGTTACAATTCAAGGTTGAGTACAAAATAT
TAGGAAAATTAAAGGCAAAAGTTTCGTGATAATCCGTGATTATCAAGGAATTTTTCAAGCTGTTGTAACTCAAAATTCAA
AAGTTGATCGATTCACCAAGGAATCTGTTGTCAAAGTTCAAGGAATTCTTACTCAGCGGATAAATCCAAATACCAAAATT
CATAACGGTGATCTTGAGATTCTAGTTGATAATTTAGAGACAATTTCATTAGCCGATCAGATTCCTTTTGAAATTAGTGA
TCAGCTAGAAGTAAGCGAGGATTTACGACTACAATTTCGTTATTTGGATCTACGACGTCAAATAATGAAGGAAAATTTAA
CTTTTCGTTATAAAATTTTCCATTATATTCGTTGTTTTTTATACAAAAACAACTTTATTGAAATTGAAACTCCAATTCTT
TCTAAATCAACACCAGAAGGTGCTAGATCATTTTTAGTTCCAACACGGCAAAAAGGCAAATTTTTCGCACTTCCGCAATC
ACCACAAATTTATAAACAGCTTTTGATGGTTTCTGCTTTTGAAAAATACTTCCAGTTTGCTCGTGTTTTTCGCGATGAAG
ATCTCAGAAAAGATCGTCAATTTGAATTTTTACAGCTTGATTTAGAATTTGGATTTGCCACTTTTGAAAAAATTTCAGAG
GAAATTGAAGCACTAATACGTGATTTATGAACAAAAATTTTCCCTAATTTAGCAATAGATTTCCCAAAACTTACTTATTT
TGAAGCAATTAAGAACTATGGAACTGACAAACCTGATCTCCGTTTTGAGACTAAATTATTTGATTTAGACTTTCTTAATA
ATATAGACTTGCTTTTTCAAGGAAAAGTTCGTGGACTTTTCCTTGAAAAAGTTTTAATTACAAAACCAGAATACCGAATT
TTTGTCGAAAAAATTCAACAAAATAATGCAAAAACACTTTTATACCTGCATATTTTTGAGGGAAAAATTACTTATTTTTC
CTTTAAAACTAGTCAAAAACTCTTAATAAAAACTAAAATTGAGAACTGAATTTTGCAAAATAACTACCACACTGGCACAC
TATTTCTAATTGCTGCTGATTATAAGATTGCTTCGCAGGCACTTGGAGCACTTAGAAATTTAGTCGCAAAAAAATATAAA
CTGATTAACGGGGATCAATTTAAATTTGCCTGAATTATTAACTGGCCACTTTTTGAATTAGATATAAATAACAAACTTAC
CTCAGCTCACCATCCTTTTACTGCCCCAACTTTAGAAAGTCAACAATTTTTGCATAAAAATCCTTTAAAAGTCCAAGCTC
AGGCATATGATCTTGTTCTTAATGGGTTTGAATTAGGCTCAGGGTCAATTCGAATTCACAATGTGGAATTACAAAAGCAA
GTTTTTACAATTCTCGGGCTTGATCAAGACCAAATTCATACTCAATTTGGAGCGCTGTTACGCGCATTTAATTACGGCGT
TCCTCCTCATGGTGGCTTCGCTTTTGGCCTTGATCGACTCATTATGATTTTAAGAAAAACGAATTCGATCCGTGATGTTA
TTGCTTTTCCGGTGAATTCAAAAGGTTTAGACCTATTGCTTGATTCACCAACTGAAATTGACCAAAATTTACTTGCTGAA
TTTGGTTTTAAGAAGACTGACAAAAGCTAA

Upstream 100 bases:

>100_bases
AAAAATTATATTTCTCTAAAAAATTTGCAAACTGGAAAGAATGAACAAATTTTATATACTGAAATTAATTTTGAATATCT
AAATTCAATTATCAAAGCGA

Downstream 100 bases:

>100_bases
ACTACCAAAAAAAATAAATAAAAATTATTTTTTTTGGTATAATTTATTATATAATTTTATAAGTAGTTGGGCAAAAATGA
TATATGATAAAATAGAAAAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MKMHNSKTLSKSDLGKTVTIQGWVQNIRKIKGKSFVIIRDYQGIFQAVVTQNSKVDRFTKESVVKVQGILTQRINPNTKI
HNGDLEILVDNLETISLADQIPFEISDQLEVSEDLRLQFRYLDLRRQIMKENLTFRYKIFHYIRCFLYKNNFIEIETPIL
SKSTPEGARSFLVPTRQKGKFFALPQSPQIYKQLLMVSAFEKYFQFARVFRDEDLRKDRQFEFLQLDLEFGFATFEKISE
EIEALIRDLWTKIFPNLAIDFPKLTYFEAIKNYGTDKPDLRFETKLFDLDFLNNIDLLFQGKVRGLFLEKVLITKPEYRI
FVEKIQQNNAKTLLYLHIFEGKITYFSFKTSQKLLIKTKIENWILQNNYHTGTLFLIAADYKIASQALGALRNLVAKKYK
LINGDQFKFAWIINWPLFELDINNKLTSAHHPFTAPTLESQQFLHKNPLKVQAQAYDLVLNGFELGSGSIRIHNVELQKQ
VFTILGLDQDQIHTQFGALLRAFNYGVPPHGGFAFGLDRLIMILRKTNSIRDVIAFPVNSKGLDLLLDSPTEIDQNLLAE
FGFKKTDKS

Sequences:

>Translated_569_residues
MKMHNSKTLSKSDLGKTVTIQG*VQNIRKIKGKSFVIIRDYQGIFQAVVTQNSKVDRFTKESVVKVQGILTQRINPNTKI
HNGDLEILVDNLETISLADQIPFEISDQLEVSEDLRLQFRYLDLRRQIMKENLTFRYKIFHYIRCFLYKNNFIEIETPIL
SKSTPEGARSFLVPTRQKGKFFALPQSPQIYKQLLMVSAFEKYFQFARVFRDEDLRKDRQFEFLQLDLEFGFATFEKISE
EIEALIRDL*TKIFPNLAIDFPKLTYFEAIKNYGTDKPDLRFETKLFDLDFLNNIDLLFQGKVRGLFLEKVLITKPEYRI
FVEKIQQNNAKTLLYLHIFEGKITYFSFKTSQKLLIKTKIEN*ILQNNYHTGTLFLIAADYKIASQALGALRNLVAKKYK
LINGDQFKFA*IINWPLFELDINNKLTSAHHPFTAPTLESQQFLHKNPLKVQAQAYDLVLNGFELGSGSIRIHNVELQKQ
VFTILGLDQDQIHTQFGALLRAFNYGVPPHGGFAFGLDRLIMILRKTNSIRDVIAFPVNSKGLDLLLDSPTEIDQNLLAE
FGFKKTDKS
>Mature_569_residues
MKMHNSKTLSKSDLGKTVTIQG*VQNIRKIKGKSFVIIRDYQGIFQAVVTQNSKVDRFTKESVVKVQGILTQRINPNTKI
HNGDLEILVDNLETISLADQIPFEISDQLEVSEDLRLQFRYLDLRRQIMKENLTFRYKIFHYIRCFLYKNNFIEIETPIL
SKSTPEGARSFLVPTRQKGKFFALPQSPQIYKQLLMVSAFEKYFQFARVFRDEDLRKDRQFEFLQLDLEFGFATFEKISE
EIEALIRDL*TKIFPNLAIDFPKLTYFEAIKNYGTDKPDLRFETKLFDLDFLNNIDLLFQGKVRGLFLEKVLITKPEYRI
FVEKIQQNNAKTLLYLHIFEGKITYFSFKTSQKLLIKTKIEN*ILQNNYHTGTLFLIAADYKIASQALGALRNLVAKKYK
LINGDQFKFA*IINWPLFELDINNKLTSAHHPFTAPTLESQQFLHKNPLKVQAQAYDLVLNGFELGSGSIRIHNVELQKQ
VFTILGLDQDQIHTQFGALLRAFNYGVPPHGGFAFGLDRLIMILRKTNSIRDVIAFPVNSKGLDLLLDSPTEIDQNLLAE
FGFKKTDKS

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=35.8931552587646, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI45439306, Length=262, Percent_Identity=30.9160305343511, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=38.5786802030457, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1790571, Length=296, Percent_Identity=29.3918918918919, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789256, Length=295, Percent_Identity=28.4745762711864, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=586, Percent_Identity=34.3003412969283, Blast_Score=300, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=28.9285714285714, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=619, Percent_Identity=33.4410339256866, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6323011, Length=291, Percent_Identity=26.4604810996564, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=244, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24584738, Length=588, Percent_Identity=36.0544217687075, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI17136276, Length=242, Percent_Identity=28.9256198347107, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 65391; Mature: 65391

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMHNSKTLSKSDLGKTVTIQGVQNIRKIKGKSFVIIRDYQGIFQAVVTQNSKVDRFTKE
CCCCCCCCCCHHHCCCEEEEHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCHHHHHHH
SVVKVQGILTQRINPNTKIHNGDLEILVDNLETISLADQIPFEISDQLEVSEDLRLQFRY
HHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEEEHHCCCCCCCCCCCCCCCCHHHHHHH
LDLRRQIMKENLTFRYKIFHYIRCFLYKNNFIEIETPILSKSTPEGARSFLVPTRQKGKF
HHHHHHHHHCCCCEEEHHHHHHHHHEEECCEEEEECCCCCCCCCCHHHHCCCCCCCCCCE
FALPQSPQIYKQLLMVSAFEKYFQFARVFRDEDLRKDRQFEFLQLDLEFGFATFEKISEE
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEEECCCHHHHHHHHHH
IEALIRDLTKIFPNLAIDFPKLTYFEAIKNYGTDKPDLRFETKLFDLDFLNNIDLLFQGK
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEEEEEHHCCCCCEEEECC
VRGLFLEKVLITKPEYRIFVEKIQQNNAKTLLYLHIFEGKITYFSFKTSQKLLIKTKIEN
HHHEEHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCEEEHHHHHHH
ILQNNYHTGTLFLIAADYKIASQALGALRNLVAKKYKLINGDQFKFAIINWPLFELDINN
HHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEEEEECCC
KLTSAHHPFTAPTLESQQFLHKNPLKVQAQAYDLVLNGFELGSGSIRIHNVELQKQVFTI
CCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEEEEEEECEEECCCCEEEEEEEHHHHHHHH
LGLDQDQIHTQFGALLRAFNYGVPPHGGFAFGLDRLIMILRKTNSIRDVIAFPVNSKGLD
HCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCE
LLLDSPTEIDQNLLAEFGFKKTDKS
EEECCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKMHNSKTLSKSDLGKTVTIQGVQNIRKIKGKSFVIIRDYQGIFQAVVTQNSKVDRFTKE
CCCCCCCCCCHHHCCCEEEEHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCHHHHHHH
SVVKVQGILTQRINPNTKIHNGDLEILVDNLETISLADQIPFEISDQLEVSEDLRLQFRY
HHHHHHHHHHHCCCCCCEEECCCEEEEEECCCEEEHHCCCCCCCCCCCCCCCCHHHHHHH
LDLRRQIMKENLTFRYKIFHYIRCFLYKNNFIEIETPILSKSTPEGARSFLVPTRQKGKF
HHHHHHHHHCCCCEEEHHHHHHHHHEEECCEEEEECCCCCCCCCCHHHHCCCCCCCCCCE
FALPQSPQIYKQLLMVSAFEKYFQFARVFRDEDLRKDRQFEFLQLDLEFGFATFEKISEE
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEEEECCCHHHHHHHHHH
IEALIRDLTKIFPNLAIDFPKLTYFEAIKNYGTDKPDLRFETKLFDLDFLNNIDLLFQGK
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCCCCCCCEEEEEEEEEHHCCCCCEEEECC
VRGLFLEKVLITKPEYRIFVEKIQQNNAKTLLYLHIFEGKITYFSFKTSQKLLIKTKIEN
HHHEEHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCCEEEHHHHHHH
ILQNNYHTGTLFLIAADYKIASQALGALRNLVAKKYKLINGDQFKFAIINWPLFELDINN
HHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEEEEECCC
KLTSAHHPFTAPTLESQQFLHKNPLKVQAQAYDLVLNGFELGSGSIRIHNVELQKQVFTI
CCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEEEEEEECEEECCCCEEEEEEEHHHHHHHH
LGLDQDQIHTQFGALLRAFNYGVPPHGGFAFGLDRLIMILRKTNSIRDVIAFPVNSKGLD
HCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCE
LLLDSPTEIDQNLLAEFGFKKTDKS
EEECCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12466555 [H]