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The map label for this gene is miaA

Identifier: 52842902

GI number: 52842902

Start: 3048511

End: 3049476

Strand: Reverse

Name: miaA

Synonym: lpg2696

Alternate gene names: 52842902

Gene position: 3049476-3048511 (Counterclockwise)

Preceding gene: 52842903

Following gene: 52842901

Centisome position: 89.75

GC content: 40.06

Gene sequence:

>966_bases
TTGATGATTGTCGGATGTTATTGCATGAATAAGCTAGTGTTTTGTTTAATGGGACCAACTGCTTCAGGAAAGACAGGATT
AGCATGCGAGTTGCTAACTCATTTCCCTTTTGAAATTATTAGTGTTGATTCCGCCATGATTTATAGAGATATGAATATTG
GGACAGCAAAACCTTCAATCCACGAATTACAGAGAGCGCCTCATTATTTAATTGATATTAAAGATCCGGTTGAATCCTAT
TCGGCAGCTCAGTTTTGTACAGACGCTTTGTCTTTGTGTGCTGAAATCATCAAAAGAGGGAATATTCCTTTGTTAGTAGG
TGGAACTATGATGTATTTTAATGCCCTGCAAAAAGGGTTAGCCACTTTGCCTGAAGCCGATGAAGCTGTTCGTAAACGAC
TGGAGGAGGAAGCCTTATCACAGGGTTGGGATTTTCTTTATCAAAAGCTAAGTCAATTAGATCCGGTAACAGCAGCTCGC
ATTCATGCTCATGATACGCAAAGAATTCAAAGAGCGCTGGAAGTTTATTACTTAACTGGTTCAACGTTATCAACCTATTT
GACCGGACCCCATGAACGGCCTGATTATTATTTTGTTAATCTTGCTCTTTTTCCTGAGCAAAGATCCTGGTTGCATGAAC
GTATCGCGCAACGGTTTGATGCGATGCTTAGTGAGGGATTCATAGAAGAAGTTCAACAATTGCAAGCAAAATGGCCTATT
CAAATCAACTTGCCCTCCATGCGATGTGTTGGATATAGGCAAATACTCCAGCACCTTGCAGGCCACTATGATTATGAAAC
TATGCGAGAAAAAGGTATAGCAGCAACAAGGCAGCTGGCCAAACGGCAATTAACCTGGTTGAGGCATTGGGAGGGGGCTT
TATTTTATGATTCGCAAAATGTGGGATTTAATACAGATATTATAGCGAAAATAAGGGAAATATTAGATAATACTGTCTCT
AATTGA

Upstream 100 bases:

>100_bases
CCAAAACAATTAACAGTTGAAGAGCGTATTGAAATGAATCAATTGTTGCTGGAACTCTCTCTTCAAGAAGAGAAATGTAA
TACATGTAAAGCGTTAACCA

Downstream 100 bases:

>100_bases
CATCATGCCGCAAAGGAACAACATGAGGAAAACAACAAAAGAACCTGCAAGTGCTAAAAAAAATTATGTCGTACATAGAC
ATCAATTACCATTGAGTTGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MMIVGCYCMNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQRAPHYLIDIKDPVESY
SAAQFCTDALSLCAEIIKRGNIPLLVGGTMMYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAAR
IHAHDTQRIQRALEVYYLTGSTLSTYLTGPHERPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPI
QINLPSMRCVGYRQILQHLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVS
N

Sequences:

>Translated_321_residues
MMIVGCYCMNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQRAPHYLIDIKDPVESY
SAAQFCTDALSLCAEIIKRGNIPLLVGGTMMYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAAR
IHAHDTQRIQRALEVYYLTGSTLSTYLTGPHERPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPI
QINLPSMRCVGYRQILQHLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVS
N
>Mature_321_residues
MMIVGCYCMNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSIHELQRAPHYLIDIKDPVESY
SAAQFCTDALSLCAEIIKRGNIPLLVGGTMMYFNALQKGLATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAAR
IHAHDTQRIQRALEVYYLTGSTLSTYLTGPHERPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPI
QINLPSMRCVGYRQILQHLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQNVGFNTDIIAKIREILDNTVS
N

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=28.7539936102236, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1790613, Length=300, Percent_Identity=51.6666666666667, Blast_Score=311, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI25144712, Length=269, Percent_Identity=24.907063197026, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=298, Percent_Identity=32.8859060402685, Blast_Score=145, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24649830, Length=236, Percent_Identity=31.3559322033898, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36677; Mature: 36677

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIVGCYCMNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSI
CEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHEEECCHHHHEEECCCCCCCCHH
HELQRAPHYLIDIKDPVESYSAAQFCTDALSLCAEIIKRGNIPLLVGGTMMYFNALQKGL
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHH
ATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQRIQRALEVYYLTG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEC
STLSTYLTGPHERPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPI
CHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
QINLPSMRCVGYRQILQHLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQN
EECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
VGFNTDIIAKIREILDNTVSN
CCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMIVGCYCMNKLVFCLMGPTASGKTGLACELLTHFPFEIISVDSAMIYRDMNIGTAKPSI
CEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHEEECCHHHHEEECCCCCCCCHH
HELQRAPHYLIDIKDPVESYSAAQFCTDALSLCAEIIKRGNIPLLVGGTMMYFNALQKGL
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHH
ATLPEADEAVRKRLEEEALSQGWDFLYQKLSQLDPVTAARIHAHDTQRIQRALEVYYLTG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEC
STLSTYLTGPHERPDYYFVNLALFPEQRSWLHERIAQRFDAMLSEGFIEEVQQLQAKWPI
CHHHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
QINLPSMRCVGYRQILQHLAGHYDYETMREKGIAATRQLAKRQLTWLRHWEGALFYDSQN
EECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
VGFNTDIIAKIREILDNTVSN
CCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA