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The map label for this gene is gidA [H]

Identifier: 52788046

GI number: 52788046

Start: 4216534

End: 4218420

Strand: Reverse

Name: gidA [H]

Synonym: BLi04374

Alternate gene names: 52788046

Gene position: 4218420-4216534 (Counterclockwise)

Preceding gene: 52788047

Following gene: 52788045

Centisome position: 99.9

GC content: 49.55

Gene sequence:

>1887_bases
ATGGGCTATGAAGCAGGCCAATATGACGTCGTTGTCGTCGGCGCCGGACACGCCGGTGTTGAAGCGGCGCTGGCGTCAGC
CAGACAAGGCGCAAAAACGCTTGTCTTAACGATTAACCTTGATATGGTTGCTTTCATGCCATGTAACCCGTCTGTCGGCG
GACCTGCAAAAGGAATCGTCGTCCGCGAAATTGACGCGCTCGGCGGGGAAATGGCTAAAAATATTGATAAAACGCATATC
CAAATGAGGCTTTTAAATACAGGAAAAGGACCTGCCGTTCGGGCGCTTCGGGCTCAGGCTGATAAATTCCAATATCAGCA
TGAAATGAAAAAGACACTTGAGAATGAACCGAATTTGACCATGCTGCAAGGAATGGTCGAACGCCTTCTTATTGAAGACG
GGGAATGCCGGGGCGTTATAACCCAGACAGGGGCGGAGTACCGTTCTAAGACCGTTGTTCTGACGACGGGGACATTTTTG
AGAGGGAAAATCATTCTTGGCGATCTGTCATACTCCAGCGGACCGAACAACCAGCAGCCGTCCATCAAGCTGTCTGAACA
TCTTGAAGAGCTCGGCTTTGACCTCGTCCGTTTTAAAACAGGGACGCCGCCGCGGGTCAACAGTCATTCAATTGACTACA
GCAAAACGGAAATCCAGCCCGGTGATGAAGTGCCGCGGGCCTTTTCGTATGAGACGGTGGAATATATTACAGACCAGCTT
CCATGCTGGCTCACATATACAAGTCCGGAAACGCACGAAATCATTGACAACAATTTGCATCGTTCACCGATGTACTCCGG
CATGATCAAAGGAACGGGACCGAGATATTGCCCGTCTATTGAAGACAAGGTCGTCCGCTTTAACGACAAGCCGAGACACC
AAATCTTTCTTGAGCCTGAAGGGCGCAACACCCAGGAAGTCTATGTCCAGGGATTGTCGACAAGCCTTCCAGAAGATGTG
CAGCGAAAAATGCTTTCAACTATTCCCGGCCTTGAGAATGTGCAAATGATGCGGGCGGGGTATGCGATTGAATATGATGC
GATCGTACCGACACAGCTGTGGCCGACATTGGAAACGAAAAAAATTCCGGGACTCTTCACAGCCGGACAGATTAACGGAA
CATCAGGCTATGAAGAAGCGGCCGGACAGGGAATTATGGCCGGTATCAATGCGGGCCGGAAAGCGCTTGGCAAAGAAGAG
GTCATTCTCAGCCGCTCAGACGCTTATATCGGCGTATTGATCGACGACCTTGTGACAAAAGGAACAAACGAACCATACCG
CCTGCTGACTTCGCGTGCAGAGTACAGGCTGCTCCTGCGTCACGACAATGCAGACTTGCGCCTGACCGAGATCGGCTATC
AGATCGGCTTGATTTCTGAAGAAAGATATCAAAAGTTTCAAGAGAAAAAAGCGGCCATTGAAGCAGAGAAAAAACGCCTT
CATTCTGTTATTATCAAACCGACAAAGGAAAATCAGGAATATATCCGTTCACTGGGCGGCAGCGAGCTCAAAGACGGCAT
TCGCGCGACCGACCTGATGAAGCGGCCTGAAATGAATTATGAAACCGTCACTGCTCTTGCGCCGCCTGAACAAAAAGTCG
CCGGCGACGTTGCCGAACAGGTGGAAATTCAAATTAAGTATGAAGGATATATTGAAAAGTCCTTGCAGCAGGTCGAAAAA
CTAAAGAAAATGGAAAATAAAAAGATTCCTGACAGAATCGACTATGACGCGATCAAAGGCATTGCAACGGAAGCGCGCCA
AAAATTGAAAGAGGTCAGACCGCTGTCTGTCGCACAGGCCTCACGGATTTCCGGCGTCAATCCTGCTGATATTTCCATAC
TGCTCGTTTATTTAGAGCAGGGACGGATTGCCAAGGTTGCGGAATAG

Upstream 100 bases:

>100_bases
GAAGTTCTTGGGGAGATCATTGGCGATGCTGTACACGAAAGTCTGATCGACCAGCTCTTCTCTCAATTTTGCTTAGGAAA
ATAAAGGAGGAACAAAGCAT

Downstream 100 bases:

>100_bases
AAAGGGTGACGGCATGAACATTGAGCAATTTACAGCCGGTTTGGAGAAGAAAGGCATTTCCCTTTCCTCCTTCCAGCTGG
AGCAATTTGAGACTTATTAC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 628; Mature: 627

Protein sequence:

>628_residues
MGYEAGQYDVVVVGAGHAGVEAALASARQGAKTLVLTINLDMVAFMPCNPSVGGPAKGIVVREIDALGGEMAKNIDKTHI
QMRLLNTGKGPAVRALRAQADKFQYQHEMKKTLENEPNLTMLQGMVERLLIEDGECRGVITQTGAEYRSKTVVLTTGTFL
RGKIILGDLSYSSGPNNQQPSIKLSEHLEELGFDLVRFKTGTPPRVNSHSIDYSKTEIQPGDEVPRAFSYETVEYITDQL
PCWLTYTSPETHEIIDNNLHRSPMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRNTQEVYVQGLSTSLPEDV
QRKMLSTIPGLENVQMMRAGYAIEYDAIVPTQLWPTLETKKIPGLFTAGQINGTSGYEEAAGQGIMAGINAGRKALGKEE
VILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTEIGYQIGLISEERYQKFQEKKAAIEAEKKRL
HSVIIKPTKENQEYIRSLGGSELKDGIRATDLMKRPEMNYETVTALAPPEQKVAGDVAEQVEIQIKYEGYIEKSLQQVEK
LKKMENKKIPDRIDYDAIKGIATEARQKLKEVRPLSVAQASRISGVNPADISILLVYLEQGRIAKVAE

Sequences:

>Translated_628_residues
MGYEAGQYDVVVVGAGHAGVEAALASARQGAKTLVLTINLDMVAFMPCNPSVGGPAKGIVVREIDALGGEMAKNIDKTHI
QMRLLNTGKGPAVRALRAQADKFQYQHEMKKTLENEPNLTMLQGMVERLLIEDGECRGVITQTGAEYRSKTVVLTTGTFL
RGKIILGDLSYSSGPNNQQPSIKLSEHLEELGFDLVRFKTGTPPRVNSHSIDYSKTEIQPGDEVPRAFSYETVEYITDQL
PCWLTYTSPETHEIIDNNLHRSPMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRNTQEVYVQGLSTSLPEDV
QRKMLSTIPGLENVQMMRAGYAIEYDAIVPTQLWPTLETKKIPGLFTAGQINGTSGYEEAAGQGIMAGINAGRKALGKEE
VILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTEIGYQIGLISEERYQKFQEKKAAIEAEKKRL
HSVIIKPTKENQEYIRSLGGSELKDGIRATDLMKRPEMNYETVTALAPPEQKVAGDVAEQVEIQIKYEGYIEKSLQQVEK
LKKMENKKIPDRIDYDAIKGIATEARQKLKEVRPLSVAQASRISGVNPADISILLVYLEQGRIAKVAE
>Mature_627_residues
GYEAGQYDVVVVGAGHAGVEAALASARQGAKTLVLTINLDMVAFMPCNPSVGGPAKGIVVREIDALGGEMAKNIDKTHIQ
MRLLNTGKGPAVRALRAQADKFQYQHEMKKTLENEPNLTMLQGMVERLLIEDGECRGVITQTGAEYRSKTVVLTTGTFLR
GKIILGDLSYSSGPNNQQPSIKLSEHLEELGFDLVRFKTGTPPRVNSHSIDYSKTEIQPGDEVPRAFSYETVEYITDQLP
CWLTYTSPETHEIIDNNLHRSPMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPEGRNTQEVYVQGLSTSLPEDVQ
RKMLSTIPGLENVQMMRAGYAIEYDAIVPTQLWPTLETKKIPGLFTAGQINGTSGYEEAAGQGIMAGINAGRKALGKEEV
ILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTEIGYQIGLISEERYQKFQEKKAAIEAEKKRLH
SVIIKPTKENQEYIRSLGGSELKDGIRATDLMKRPEMNYETVTALAPPEQKVAGDVAEQVEIQIKYEGYIEKSLQQVEKL
KKMENKKIPDRIDYDAIKGIATEARQKLKEVRPLSVAQASRISGVNPADISILLVYLEQGRIAKVAE

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=633, Percent_Identity=42.654028436019, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI19882217, Length=658, Percent_Identity=41.0334346504559, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI183227703, Length=673, Percent_Identity=40.1188707280832, Blast_Score=453, Evalue=1e-127,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=51.685393258427, Blast_Score=636, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=617, Percent_Identity=41.1669367909238, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6321202, Length=618, Percent_Identity=43.5275080906149, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=43.4850863422292, Blast_Score=482, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69814; Mature: 69683

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYEAGQYDVVVVGAGHAGVEAALASARQGAKTLVLTINLDMVAFMPCNPSVGGPAKGIV
CCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCCEE
VREIDALGGEMAKNIDKTHIQMRLLNTGKGPAVRALRAQADKFQYQHEMKKTLENEPNLT
EEEHHHHCHHHHHCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MLQGMVERLLIEDGECRGVITQTGAEYRSKTVVLTTGTFLRGKIILGDLSYSSGPNNQQP
HHHHHHHHHHHCCCCCCEEEECCCHHHHCCEEEEEECCEEEEEEEEEECCCCCCCCCCCC
SIKLSEHLEELGFDLVRFKTGTPPRVNSHSIDYSKTEIQPGDEVPRAFSYETVEYITDQL
CEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
PCWLTYTSPETHEIIDNNLHRSPMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPE
CEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCC
GRNTQEVYVQGLSTSLPEDVQRKMLSTIPGLENVQMMRAGYAIEYDAIVPTQLWPTLETK
CCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCHHCCCCCCC
KIPGLFTAGQINGTSGYEEAAGQGIMAGINAGRKALGKEEVILSRSDAYIGVLIDDLVTK
CCCCEEEECCCCCCCCHHHHCCCCEEECCCHHHHHCCCCCEEEECCCCEEEEEHHHHHHC
GTNEPYRLLTSRAEYRLLLRHDNADLRLTEIGYQIGLISEERYQKFQEKKAAIEAEKKRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEEECEEEEECCHHHHHHHHHHHHHHHHHHHHH
HSVIIKPTKENQEYIRSLGGSELKDGIRATDLMKRPEMNYETVTALAPPEQKVAGDVAEQ
HHEEECCCCCCHHHHHHCCCHHHHHCHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCEE
VEIQIKYEGYIEKSLQQVEKLKKMENKKIPDRIDYDAIKGIATEARQKLKEVRPLSVAQA
EEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
SRISGVNPADISILLVYLEQGRIAKVAE
HHCCCCCCCCCEEEEEEECCCCEEECCC
>Mature Secondary Structure 
GYEAGQYDVVVVGAGHAGVEAALASARQGAKTLVLTINLDMVAFMPCNPSVGGPAKGIV
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCCCCCCCCCEE
VREIDALGGEMAKNIDKTHIQMRLLNTGKGPAVRALRAQADKFQYQHEMKKTLENEPNLT
EEEHHHHCHHHHHCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MLQGMVERLLIEDGECRGVITQTGAEYRSKTVVLTTGTFLRGKIILGDLSYSSGPNNQQP
HHHHHHHHHHHCCCCCCEEEECCCHHHHCCEEEEEECCEEEEEEEEEECCCCCCCCCCCC
SIKLSEHLEELGFDLVRFKTGTPPRVNSHSIDYSKTEIQPGDEVPRAFSYETVEYITDQL
CEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
PCWLTYTSPETHEIIDNNLHRSPMYSGMIKGTGPRYCPSIEDKVVRFNDKPRHQIFLEPE
CEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCC
GRNTQEVYVQGLSTSLPEDVQRKMLSTIPGLENVQMMRAGYAIEYDAIVPTQLWPTLETK
CCCHHHHHEECCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCHHCCCCCCC
KIPGLFTAGQINGTSGYEEAAGQGIMAGINAGRKALGKEEVILSRSDAYIGVLIDDLVTK
CCCCEEEECCCCCCCCHHHHCCCCEEECCCHHHHHCCCCCEEEECCCCEEEEEHHHHHHC
GTNEPYRLLTSRAEYRLLLRHDNADLRLTEIGYQIGLISEERYQKFQEKKAAIEAEKKRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEEECEEEEECCHHHHHHHHHHHHHHHHHHHHH
HSVIIKPTKENQEYIRSLGGSELKDGIRATDLMKRPEMNYETVTALAPPEQKVAGDVAEQ
HHEEECCCCCCHHHHHHCCCHHHHHCHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCEE
VEIQIKYEGYIEKSLQQVEKLKKMENKKIPDRIDYDAIKGIATEARQKLKEVRPLSVAQA
EEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
SRISGVNPADISILLVYLEQGRIAKVAE
HHCCCCCCCCCEEEEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA