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The map label for this gene is gidB

Identifier: 52788045

GI number: 52788045

Start: 4215801

End: 4216520

Strand: Reverse

Name: gidB

Synonym: BLi04373

Alternate gene names: 52788045

Gene position: 4216520-4215801 (Counterclockwise)

Preceding gene: 52788046

Following gene: 52788044

Centisome position: 99.85

GC content: 42.78

Gene sequence:

>720_bases
ATGAACATTGAGCAATTTACAGCCGGTTTGGAGAAGAAAGGCATTTCCCTTTCCTCCTTCCAGCTGGAGCAATTTGAGAC
TTATTACGAATGGCTTGTTGAATGGAACGAAAAAATGAATTTAACCTCGATTACCGAGAAAAAAGAAGTGTACTTAAAGC
ATTTTTACGATTCCATCGCCGCCTCTTTTTATGTTGATTTCAAAAAGATGAACACTCTCTGTGATGTTGGAGCGGGTGCC
GGATTTCCAAGCCTTCCGATTAAAATCTGCTTTCCTCACCTTCACGTCACAATCGTTGACTCACTGAACAAACGGATTCA
CTTTTTGAATCAGCTGTCAGACGCTTTGAAGCTGGAAAACACAGCTTTTTACCATGACCGCGCGGAAACATTCGGAAGAT
CGAAAGACCACAGAGAAAGCTATGACGTTGTGACGGCACGCGCTGTCGCCCGCCTTTCGGTTCTCAGCGAGCTCTGCCTT
CCGCTTGTGAAAAAAGACGGTTTATTCGTAGCATTAAAAGCCGCTTCGGCTGATGAAGAAATTGAAACGGGCAAAAAAGC
CATCAAAACGCTTGGAGGCAAAATTGAAACCGTACATTCTTTTCAGCTGCCAATAGAAGAAAGCGAAAGAAACATCATTG
TCATCAAAAAACAATCGCAGACACCGAAGAAATTTCCAAGAAAGCCTGGAACACCTAATAAATCTCCTATTGAAGGTTAA

Upstream 100 bases:

>100_bases
TCACGGATTTCCGGCGTCAATCCTGCTGATATTTCCATACTGCTCGTTTATTTAGAGCAGGGACGGATTGCCAAGGTTGC
GGAATAGAAAGGGTGACGGC

Downstream 100 bases:

>100_bases
ATTATTCGTTTTCTTCAAATTTCGTGATGTCACAGAAGGAAAATTCATGAGAAAATAGAATTATAAAAATGGCAGTGTTT
AAAGGTGGTGTAGGTACATG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNIEQFTAGLEKKGISLSSFQLEQFETYYEWLVEWNEKMNLTSITEKKEVYLKHFYDSIAASFYVDFKKMNTLCDVGAGA
GFPSLPIKICFPHLHVTIVDSLNKRIHFLNQLSDALKLENTAFYHDRAETFGRSKDHRESYDVVTARAVARLSVLSELCL
PLVKKDGLFVALKAASADEEIETGKKAIKTLGGKIETVHSFQLPIEESERNIIVIKKQSQTPKKFPRKPGTPNKSPIEG

Sequences:

>Translated_239_residues
MNIEQFTAGLEKKGISLSSFQLEQFETYYEWLVEWNEKMNLTSITEKKEVYLKHFYDSIAASFYVDFKKMNTLCDVGAGA
GFPSLPIKICFPHLHVTIVDSLNKRIHFLNQLSDALKLENTAFYHDRAETFGRSKDHRESYDVVTARAVARLSVLSELCL
PLVKKDGLFVALKAASADEEIETGKKAIKTLGGKIETVHSFQLPIEESERNIIVIKKQSQTPKKFPRKPGTPNKSPIEG
>Mature_239_residues
MNIEQFTAGLEKKGISLSSFQLEQFETYYEWLVEWNEKMNLTSITEKKEVYLKHFYDSIAASFYVDFKKMNTLCDVGAGA
GFPSLPIKICFPHLHVTIVDSLNKRIHFLNQLSDALKLENTAFYHDRAETFGRSKDHRESYDVVTARAVARLSVLSELCL
PLVKKDGLFVALKAASADEEIETGKKAIKTLGGKIETVHSFQLPIEESERNIIVIKKQSQTPKKFPRKPGTPNKSPIEG

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=207, Percent_Identity=35.2657004830918, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_BACLD (Q65CN3)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_081437.1
- RefSeq:   YP_093874.1
- ProteinModelPortal:   Q65CN3
- SMR:   Q65CN3
- STRING:   Q65CN3
- EnsemblBacteria:   EBBACT00000056222
- EnsemblBacteria:   EBBACT00000062106
- GeneID:   3029937
- GeneID:   3098295
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04373
- KEGG:   bli:BL00108
- NMPDR:   fig|279010.5.peg.3902
- eggNOG:   COG0357
- GeneTree:   EBGT00050000000933
- HOGENOM:   HBG686577
- OMA:   HLWDSLR
- ProtClustDB:   PRK00107
- BioCyc:   BLIC279010-1:BLI04373-MONOMER
- BioCyc:   BLIC279010:BL00108-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27073; Mature: 27073

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEQFTAGLEKKGISLSSFQLEQFETYYEWLVEWNEKMNLTSITEKKEVYLKHFYDSIA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ASFYVDFKKMNTLCDVGAGAGFPSLPIKICFPHLHVTIVDSLNKRIHFLNQLSDALKLEN
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TAFYHDRAETFGRSKDHRESYDVVTARAVARLSVLSELCLPLVKKDGLFVALKAASADEE
CHHEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
IETGKKAIKTLGGKIETVHSFQLPIEESERNIIVIKKQSQTPKKFPRKPGTPNKSPIEG
HHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIEQFTAGLEKKGISLSSFQLEQFETYYEWLVEWNEKMNLTSITEKKEVYLKHFYDSIA
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ASFYVDFKKMNTLCDVGAGAGFPSLPIKICFPHLHVTIVDSLNKRIHFLNQLSDALKLEN
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TAFYHDRAETFGRSKDHRESYDVVTARAVARLSVLSELCLPLVKKDGLFVALKAASADEE
CHHEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
IETGKKAIKTLGGKIETVHSFQLPIEESERNIIVIKKQSQTPKKFPRKPGTPNKSPIEG
HHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA