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The map label for this gene is trmE

Identifier: 52788047

GI number: 52788047

Start: 4218437

End: 4219816

Strand: Reverse

Name: trmE

Synonym: BLi04375

Alternate gene names: 52788047

Gene position: 4219816-4218437 (Counterclockwise)

Preceding gene: 52788048

Following gene: 52788046

Centisome position: 99.93

GC content: 48.55

Gene sequence:

>1380_bases
ATGGATACCATCGCTGCGATTTCAACGCCGATGGGAGAAGGGGCGATTGCGATCGTCCGGATGAGCGGCCCTGAGGCTCT
TGCGATCGCCGATAAAGTGTATAAAGGACCTCGCGGAAAAAGGCTGAGTTCCGTCGATTCACATACGATTAACTACGGTC
ATATCGTTGATCCGGAAACGGAAAAAGTCGTGGAAGAAGTCATGGTTTCGGTACTGAAAGCGCCGAAAACTTTTACGCGG
GAAGATATTGTAGAAATCAACTGCCACGGCGGACTTGTGACTGTCAACCAGGTGCTTCAGCTCGTTTTGAGAGAGGGAGC
GCGTCTGGCGGAACCGGGAGAATTTACGAAAAGAGCCTTTTTGAACGGAAGAATCGATCTTTCCCAGGCTGAAGCTGTCA
TGGATCTCATCAGGGCCAAAACAGACCGGGCAATGAATGTGGCCATGAATCAGATGGAAGGAAGGCTGTCTTCACTGATC
AAACGGTTGAGGGCCGAGATTCTTGAAACATTGGCCCACGTTGAAGTGAATATTGACTATCCGGAATACGATGACGTTGA
AGAAATGACGCACAAGATGCTGATTGAAAAAGCGACAAAGGTCAAAAAGGAAATCGAGGCGCTGCTGACGACTTCAGAGC
AGGGTAAAATATTAAGAGAAGGCATCTCGACAGTTATCATCGGGCGGCCCAATGTCGGCAAGTCCTCGCTTTTAAACAGT
CTCGTCCACGAGACAAAAGCGATCGTAACGGATATTCCGGGCACGACGCGGGATGTCATTGAAGAATATGTGAATGTCAG
AGGCGTGCCGCTGCGTCTCGTCGATACAGCCGGGATCCGCGAGACCGAAGACATCGTCGAGCGAATCGGCGTTGAACGTT
CAAGACAGGTATTAAAAGAAGCAGATTTGATTTTGCTTGTCCTCAATTACAGCGAGTCCCTTTCAGATGAAGACATCAAG
CTCTTTGAAGCAACGAAGGGAATGGACATCATCGTCATTGTCAATAAGACCGATCTTGAGCAAAAGCTTGATCTTGATCG
GGTTCGGGAATTGGCTGGGAATCAGCCTGTCGTGACGACTTCCTTATTAAAAGAGGAAGGGATTGATGAGCTGGAGGAAG
CCATTCAATCGCTGTTTTTCACAGGCGCGATCGAGAGCGGCGACTTGACATATGTCAGCAATACAAGGCATATTTCACTG
CTGCATGAGGCGAAGCGCGCCATCACTGACGCATTGGAGGGCATCGAAAACGATGTGCCGATCGATATGGTTCAAATTGA
TTTAACAAGATGCTGGGAAGTTCTTGGGGAGATCATTGGCGATGCTGTACACGAAAGTCTGATCGACCAGCTCTTCTCTC
AATTTTGCTTAGGAAAATAA

Upstream 100 bases:

>100_bases
TTTGATTACAATAAAAGTTTTTCATCCGTATACGATGTTTGGAAGATGACCCGGCAAATGGGGATAATAGATATAGGAAT
GAAAAAGAGAGGTGAAAGCC

Downstream 100 bases:

>100_bases
AGGAGGAACAAAGCATATGGGCTATGAAGCAGGCCAATATGACGTCGTTGTCGTCGGCGCCGGACACGCCGGTGTTGAAG
CGGCGCTGGCGTCAGCCAGA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDTIAAISTPMGEGAIAIVRMSGPEALAIADKVYKGPRGKRLSSVDSHTINYGHIVDPETEKVVEEVMVSVLKAPKTFTR
EDIVEINCHGGLVTVNQVLQLVLREGARLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSSLI
KRLRAEILETLAHVEVNIDYPEYDDVEEMTHKMLIEKATKVKKEIEALLTTSEQGKILREGISTVIIGRPNVGKSSLLNS
LVHETKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKEADLILLVLNYSESLSDEDIK
LFEATKGMDIIVIVNKTDLEQKLDLDRVRELAGNQPVVTTSLLKEEGIDELEEAIQSLFFTGAIESGDLTYVSNTRHISL
LHEAKRAITDALEGIENDVPIDMVQIDLTRCWEVLGEIIGDAVHESLIDQLFSQFCLGK

Sequences:

>Translated_459_residues
MDTIAAISTPMGEGAIAIVRMSGPEALAIADKVYKGPRGKRLSSVDSHTINYGHIVDPETEKVVEEVMVSVLKAPKTFTR
EDIVEINCHGGLVTVNQVLQLVLREGARLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSSLI
KRLRAEILETLAHVEVNIDYPEYDDVEEMTHKMLIEKATKVKKEIEALLTTSEQGKILREGISTVIIGRPNVGKSSLLNS
LVHETKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKEADLILLVLNYSESLSDEDIK
LFEATKGMDIIVIVNKTDLEQKLDLDRVRELAGNQPVVTTSLLKEEGIDELEEAIQSLFFTGAIESGDLTYVSNTRHISL
LHEAKRAITDALEGIENDVPIDMVQIDLTRCWEVLGEIIGDAVHESLIDQLFSQFCLGK
>Mature_459_residues
MDTIAAISTPMGEGAIAIVRMSGPEALAIADKVYKGPRGKRLSSVDSHTINYGHIVDPETEKVVEEVMVSVLKAPKTFTR
EDIVEINCHGGLVTVNQVLQLVLREGARLAEPGEFTKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSSLI
KRLRAEILETLAHVEVNIDYPEYDDVEEMTHKMLIEKATKVKKEIEALLTTSEQGKILREGISTVIIGRPNVGKSSLLNS
LVHETKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKEADLILLVLNYSESLSDEDIK
LFEATKGMDIIVIVNKTDLEQKLDLDRVRELAGNQPVVTTSLLKEEGIDELEEAIQSLFFTGAIESGDLTYVSNTRHISL
LHEAKRAITDALEGIENDVPIDMVQIDLTRCWEVLGEIIGDAVHESLIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=33.6170212765957, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=32.0717131474104, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=32.8976034858388, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=32.9764453961456, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=39.344262295082, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=467, Percent_Identity=31.0492505353319, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323665, Length=499, Percent_Identity=31.8637274549098, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45550813, Length=490, Percent_Identity=32.2448979591837, Blast_Score=215, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BACLD (Q65CN1)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_081439.1
- RefSeq:   YP_093876.1
- HSSP:   Q9X1F8
- ProteinModelPortal:   Q65CN1
- SMR:   Q65CN1
- STRING:   Q65CN1
- EnsemblBacteria:   EBBACT00000057045
- EnsemblBacteria:   EBBACT00000059968
- GeneID:   3029930
- GeneID:   3098037
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi04375
- KEGG:   bli:BL00110
- NMPDR:   fig|279010.5.peg.3957
- eggNOG:   COG0486
- GeneTree:   EBGT00070000032012
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   BLIC279010-1:BLI04375-MONOMER
- BioCyc:   BLIC279010:BL00110-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50886; Mature: 50886

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 85-85 BINDING 124-124 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIAAISTPMGEGAIAIVRMSGPEALAIADKVYKGPRGKRLSSVDSHTINYGHIVDPET
CCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
EKVVEEVMVSVLKAPKTFTREDIVEINCHGGLVTVNQVLQLVLREGARLAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSSLIKRLRAEILETLAHVEVNIDY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PEYDDVEEMTHKMLIEKATKVKKEIEALLTTSEQGKILREGISTVIIGRPNVGKSSLLNS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHHHHHH
LVHETKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKE
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHH
ADLILLVLNYSESLSDEDIKLFEATKGMDIIVIVNKTDLEQKLDLDRVRELAGNQPVVTT
CCEEEEEEECCCCCCCCCHHHEECCCCCEEEEEEECCCCHHHCCHHHHHHHHCCCCEEHH
SLLKEEGIDELEEAIQSLFFTGAIESGDLTYVSNTRHISLLHEAKRAITDALEGIENDVP
HHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
IDMVQIDLTRCWEVLGEIIGDAVHESLIDQLFSQFCLGK
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTIAAISTPMGEGAIAIVRMSGPEALAIADKVYKGPRGKRLSSVDSHTINYGHIVDPET
CCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
EKVVEEVMVSVLKAPKTFTREDIVEINCHGGLVTVNQVLQLVLREGARLAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHH
LNGRIDLSQAEAVMDLIRAKTDRAMNVAMNQMEGRLSSLIKRLRAEILETLAHVEVNIDY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PEYDDVEEMTHKMLIEKATKVKKEIEALLTTSEQGKILREGISTVIIGRPNVGKSSLLNS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCHHHHHHH
LVHETKAIVTDIPGTTRDVIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKE
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHH
ADLILLVLNYSESLSDEDIKLFEATKGMDIIVIVNKTDLEQKLDLDRVRELAGNQPVVTT
CCEEEEEEECCCCCCCCCHHHEECCCCCEEEEEEECCCCHHHCCHHHHHHHHCCCCEEHH
SLLKEEGIDELEEAIQSLFFTGAIESGDLTYVSNTRHISLLHEAKRAITDALEGIENDVP
HHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
IDMVQIDLTRCWEVLGEIIGDAVHESLIDQLFSQFCLGK
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA