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The map label for this gene is nupC [H]

Identifier: 52787905

GI number: 52787905

Start: 4063387

End: 4064568

Strand: Reverse

Name: nupC [H]

Synonym: BLi04228

Alternate gene names: 52787905

Gene position: 4064568-4063387 (Counterclockwise)

Preceding gene: 52787906

Following gene: 52787904

Centisome position: 96.26

GC content: 46.79

Gene sequence:

>1182_bases
ATGAAATATCTCATCGGGCTCATTGGTTTATTTGTCGTCTTAGGGCTTGCATGGCTGGTCAGCAGCGGAAAGAAAAGAGT
GAAATACCGCCCGGTTATCGTCATGATCGTGCTGCAATTCGCATTGGGATATATATTGCTGAACACGGGTGTCGGAAACT
ATCTCGTCGGCGGTTTTGCCAAGGGCTTCGGCTATTTGCTTGAATATGCGGCAGAAGGTGTGAATTTTGTCTTTGGCGGA
CTGGTGAATGTGAATGAATCCACATTCTTCATCAGCGTACTTCTACCGATTGTTTTTATATCTGCTTTAATCGGTATTCT
GCAATATTGGAGAATCCTTCCGTTTATTATAAAATATATTGGTCTTGCGCTCAGCAAAATCAACGGGATGGGCAAACTGG
AGTCATACAATGCCGTGGCTTCGGCGATACTGGGGCAATCTGAAGTATTCATTTCGTTAAAAAAGCAGCTTGGCCTGCTG
CCTAAACACAGACTTTATACGCTGTGCGCTTCGGCGATGTCGACCGTCTCGATGTCTATCGTCGGCGCCTATATGACGAT
GCTGAAGCCCGAATACGTTGTCACCGCGCTCGTATTGAACTTATTCGGCGGATTTATTATCGCTTCGATTATCAATCCTT
ATGAAGTCGATCGAGAACATGATATGGTAGAGGTACAGGAAGAGGAAAAACAATCCTTCTTTGAAATGCTGGGCGAATAT
ATTATGGACGGCTTTAAAGTCGCCGTCGTCGTGGCCGCCATGCTGATCGGATTTGTCGCGATCATCGCAATGATTAACGG
CATTTTCAGCGCCGCACTAGGTATATCATTCCAGGAACTGCTCGGATTTATTTTTGCGCCGTTTGCCTTCCTGATGGGTA
TTCCATGGCATGAAGCGGTGAATGCTGGAAGTATCATGGCAACCAAAATGGTCTCAAATGAATTTGTCGCGATGCAGTCT
CTCGCACAAGGCAGCTTCCATTTCAGCGGCCGCACCGAAGCGATTGTTTCCGTCTTCCTCGTCTCATTTGCCAATTTCTC
ATCGATCGGTATTATCGCCGGTGCGGTTAAAGGACTGAATGAGAAACAAGGAAACGTCGTCGCGCGCTTCGGGTTGAAAT
TGCTGTACGGCGCAACGCTCGTCAGTTTCTTGACAGCGACCGTTGTAGGATTGATCTATTGA

Upstream 100 bases:

>100_bases
AAATGAAGATAACTACTAAGATCCAGTGCGGCGGGAAGCGGAAGCTTCCTGCGCGCAGATACAAAAATGTAAGCGAATAC
ATCACCAGGAGGATCAAAAC

Downstream 100 bases:

>100_bases
TAAACATTGAAAAAAGGACTGGTGATCACAATGAGAATGGTTGATATCATCACAAAAAAACAAAATGGAGAAGAATTGAC
AACAGAAGAAATTCAATTTT

Product: NupC

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKYLIGLIGLFVVLGLAWLVSSGKKRVKYRPVIVMIVLQFALGYILLNTGVGNYLVGGFAKGFGYLLEYAAEGVNFVFGG
LVNVNESTFFISVLLPIVFISALIGILQYWRILPFIIKYIGLALSKINGMGKLESYNAVASAILGQSEVFISLKKQLGLL
PKHRLYTLCASAMSTVSMSIVGAYMTMLKPEYVVTALVLNLFGGFIIASIINPYEVDREHDMVEVQEEEKQSFFEMLGEY
IMDGFKVAVVVAAMLIGFVAIIAMINGIFSAALGISFQELLGFIFAPFAFLMGIPWHEAVNAGSIMATKMVSNEFVAMQS
LAQGSFHFSGRTEAIVSVFLVSFANFSSIGIIAGAVKGLNEKQGNVVARFGLKLLYGATLVSFLTATVVGLIY

Sequences:

>Translated_393_residues
MKYLIGLIGLFVVLGLAWLVSSGKKRVKYRPVIVMIVLQFALGYILLNTGVGNYLVGGFAKGFGYLLEYAAEGVNFVFGG
LVNVNESTFFISVLLPIVFISALIGILQYWRILPFIIKYIGLALSKINGMGKLESYNAVASAILGQSEVFISLKKQLGLL
PKHRLYTLCASAMSTVSMSIVGAYMTMLKPEYVVTALVLNLFGGFIIASIINPYEVDREHDMVEVQEEEKQSFFEMLGEY
IMDGFKVAVVVAAMLIGFVAIIAMINGIFSAALGISFQELLGFIFAPFAFLMGIPWHEAVNAGSIMATKMVSNEFVAMQS
LAQGSFHFSGRTEAIVSVFLVSFANFSSIGIIAGAVKGLNEKQGNVVARFGLKLLYGATLVSFLTATVVGLIY
>Mature_393_residues
MKYLIGLIGLFVVLGLAWLVSSGKKRVKYRPVIVMIVLQFALGYILLNTGVGNYLVGGFAKGFGYLLEYAAEGVNFVFGG
LVNVNESTFFISVLLPIVFISALIGILQYWRILPFIIKYIGLALSKINGMGKLESYNAVASAILGQSEVFISLKKQLGLL
PKHRLYTLCASAMSTVSMSIVGAYMTMLKPEYVVTALVLNLFGGFIIASIINPYEVDREHDMVEVQEEEKQSFFEMLGEY
IMDGFKVAVVVAAMLIGFVAIIAMINGIFSAALGISFQELLGFIFAPFAFLMGIPWHEAVNAGSIMATKMVSNEFVAMQS
LAQGSFHFSGRTEAIVSVFLVSFANFSSIGIIAGAVKGLNEKQGNVVARFGLKLLYGATLVSFLTATVVGLIY

Specific function: Transports Nucleosides With A High Affinity Except Guanosine And Deoxyguanosine. Driven By A Proton Motive Force. [C]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI11545853, Length=418, Percent_Identity=26.3157894736842, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI42542381, Length=419, Percent_Identity=25.5369928400955, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI227116277, Length=418, Percent_Identity=22.9665071770335, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1788737, Length=392, Percent_Identity=59.6938775510204, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1788485, Length=410, Percent_Identity=31.7073170731707, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788488, Length=410, Percent_Identity=31.9512195121951, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560276, Length=391, Percent_Identity=25.3196930946292, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71991794, Length=388, Percent_Identity=22.9381443298969, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45552517, Length=400, Percent_Identity=25, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19921868, Length=400, Percent_Identity=25, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI45552519, Length=400, Percent_Identity=25, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI281360430, Length=401, Percent_Identity=24.9376558603491, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 42644; Mature: 42644

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550462; 8867804; 9384377 [H]