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The map label for this gene is leuA

Identifier: 52786690

GI number: 52786690

Start: 2845956

End: 2847512

Strand: Reverse

Name: leuA

Synonym: BLi02958

Alternate gene names: 52786690

Gene position: 2847512-2845956 (Counterclockwise)

Preceding gene: 52786691

Following gene: 52786689

Centisome position: 67.43

GC content: 49.58

Gene sequence:

>1557_bases
GTGCGAAAAATTAACTTTTTTGATACGACACTCCGTGACGGTGAACAGTCGCCGGGCGTTAATTTAAACACGCAGGAGAA
GCTGGCGATCGCCCAGCAGCTTGAAAAACTCGGGGTCAACATTATGGAAGCGGGCTTTCCCGCTTCATCCCCCGGGGATT
TCAATGCAGTAAAAGAAATCGCAAGGACGATTAAAAACTGTTCGGTAACAGGGCTTGCCCGCTCTGTCAAAGGAGATATC
GACTCGGCGTGGGAAGCGCTCAAAGACGGAGCTGAACCAAGGCTTCACATTTTTATCGCCACTTCTGACATCCACTTAAA
ATATAAGCTGAAAAAAACGCGCGAACAGGTTCTGGAACAGGCGGTCGCAATGGTGAAATACGCCAAAGAACGCTTCCCGA
TCGTCCAGTGGTCTGCCGAAGATGCGTGCCGGACTGATCTTCCGTTTTTAGCCGAGATTGTAAGCGAAGTGATCGCCGCG
GGAGCGGATGTCGTCAATCTGCCGGATACGGTCGGTTATTTAGCGCCTGCCGAGTACGGAAATATCTTCCGCTATATTAA
AGAAAATGCCCGCAATGCCGACAAAGTCCAGCTTTCAGCCCATTGCCACGATGACTTGGGCATGGCTGTCGCCAACTCTT
TGGCTGCGATTGAAAACGGAGCGGATCAAGTCGAATCGGTCATCAACGGCATAGGCGAAAGAGCGGGGAACGCCTCGCTT
GAAGAAATCGCCGTCGCCCTCCATATCCGAAAAGATTTCTACCAGGCGGAATCAACGATTCAGCTGAATGAGATTAAACG
GACAAGTGATTTAGTGAGCAAGCTGACCGGCATGGCCGTGCCGCGGAACAAAGCGGTTGTCGGCGATAATGCCTTTGCCC
ACGAATCAGGCATTCACCAGGACGGCTTCTTAAAAGAAAAAACAACATATGAAATTATTTCTCCGGAACTTGTCGGCGTG
AAAACGGATGCGCTTGTGCTCGGAAAGCATTCAGGGCGCCACGCATTTAAAGATAAGCTGCAAAACCTCGGATTCCAGCT
TGGAGAAGAAGAAATCAATAAATTCTTCAACATTTTTAAAGAGCTTACCGGAAAGAAAAAGGAATTTACGGATGAAGATC
TCATCTCGCTGATACTCGAAGAGAAAACGGCAGACCGCAAAATCGGCTATGATTTTATTTCACTGCAGGTTCATTACGGC
ACCTCCCAGGTTCCGACAGCAACAGTCTCCCTTAAAGATCAGGAAACGGATCAGGTCATCCAGGAAGCGGCGACCGGAGC
GGGAAGCGTTGAAGCGGTGTACAACACGCTGAAGCGCTGTATGGATAAAGAAGTCCAGCTCCTTGATTACCGGATTCAAT
CCAACCGCAAGGGACAGGATGCATTCGCGCAAGTATACGTCAGGGTGATGATCGATGGAAAAGAATCGGGAGGACGCGGG
GTTGCCCAGGATGTTCTCGAAGCATCTGCCAAAGCCTACTTGAATGCTGTGAACCGCTATCTCGTATTGAAGACGAATAC
GGAAGGATTGTCAAAACAGGCGGCTGTAGGATCATAA

Upstream 100 bases:

>100_bases
GCAAGCGAAAACGAACACCAAATCGAAGTGGTCGGCAGAAAGCTTCGCGAAATGATGCCGTTTGTCAAGCAGGGCAAGAA
AAAGGAAGCGGTGGTAGCCG

Downstream 100 bases:

>100_bases
CAGTAAGAAGGAAGGAGTTTGTTGAATTTGAAAAAACGAATCGCACTTTTGCCCGGTGACGGAATTGGTCCCGAAGTATT
GGACGCGGCTGTTGATGTGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAQQLEKLGVNIMEAGFPASSPGDFNAVKEIARTIKNCSVTGLARSVKGDI
DSAWEALKDGAEPRLHIFIATSDIHLKYKLKKTREQVLEQAVAMVKYAKERFPIVQWSAEDACRTDLPFLAEIVSEVIAA
GADVVNLPDTVGYLAPAEYGNIFRYIKENARNADKVQLSAHCHDDLGMAVANSLAAIENGADQVESVINGIGERAGNASL
EEIAVALHIRKDFYQAESTIQLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKTTYEIISPELVGV
KTDALVLGKHSGRHAFKDKLQNLGFQLGEEEINKFFNIFKELTGKKKEFTDEDLISLILEEKTADRKIGYDFISLQVHYG
TSQVPTATVSLKDQETDQVIQEAATGAGSVEAVYNTLKRCMDKEVQLLDYRIQSNRKGQDAFAQVYVRVMIDGKESGGRG
VAQDVLEASAKAYLNAVNRYLVLKTNTEGLSKQAAVGS

Sequences:

>Translated_518_residues
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAQQLEKLGVNIMEAGFPASSPGDFNAVKEIARTIKNCSVTGLARSVKGDI
DSAWEALKDGAEPRLHIFIATSDIHLKYKLKKTREQVLEQAVAMVKYAKERFPIVQWSAEDACRTDLPFLAEIVSEVIAA
GADVVNLPDTVGYLAPAEYGNIFRYIKENARNADKVQLSAHCHDDLGMAVANSLAAIENGADQVESVINGIGERAGNASL
EEIAVALHIRKDFYQAESTIQLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKTTYEIISPELVGV
KTDALVLGKHSGRHAFKDKLQNLGFQLGEEEINKFFNIFKELTGKKKEFTDEDLISLILEEKTADRKIGYDFISLQVHYG
TSQVPTATVSLKDQETDQVIQEAATGAGSVEAVYNTLKRCMDKEVQLLDYRIQSNRKGQDAFAQVYVRVMIDGKESGGRG
VAQDVLEASAKAYLNAVNRYLVLKTNTEGLSKQAAVGS
>Mature_518_residues
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAQQLEKLGVNIMEAGFPASSPGDFNAVKEIARTIKNCSVTGLARSVKGDI
DSAWEALKDGAEPRLHIFIATSDIHLKYKLKKTREQVLEQAVAMVKYAKERFPIVQWSAEDACRTDLPFLAEIVSEVIAA
GADVVNLPDTVGYLAPAEYGNIFRYIKENARNADKVQLSAHCHDDLGMAVANSLAAIENGADQVESVINGIGERAGNASL
EEIAVALHIRKDFYQAESTIQLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKTTYEIISPELVGV
KTDALVLGKHSGRHAFKDKLQNLGFQLGEEEINKFFNIFKELTGKKKEFTDEDLISLILEEKTADRKIGYDFISLQVHYG
TSQVPTATVSLKDQETDQVIQEAATGAGSVEAVYNTLKRCMDKEVQLLDYRIQSNRKGQDAFAQVYVRVMIDGKESGGRG
VAQDVLEASAKAYLNAVNRYLVLKTNTEGLSKQAAVGS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=499, Percent_Identity=44.4889779559118, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320071, Length=371, Percent_Identity=27.7628032345013, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=28.1420765027322, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=539, Percent_Identity=24.860853432282, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=537, Percent_Identity=23.463687150838, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_BACLD (Q65GI8)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_080102.1
- RefSeq:   YP_092519.1
- ProteinModelPortal:   Q65GI8
- SMR:   Q65GI8
- STRING:   Q65GI8
- EnsemblBacteria:   EBBACT00000057452
- EnsemblBacteria:   EBBACT00000062509
- GeneID:   3028409
- GeneID:   3098441
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02958
- KEGG:   bli:BL00611
- NMPDR:   fig|279010.5.peg.3308
- eggNOG:   COG0119
- GeneTree:   EBGT00050000000890
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   BLIC279010-1:BLI02958-MONOMER
- BioCyc:   BLIC279010:BL00611-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56861; Mature: 56861

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAQQLEKLGVNIMEAGFPASSPGDFNAVKEI
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHH
ARTIKNCSVTGLARSVKGDIDSAWEALKDGAEPRLHIFIATSDIHLKYKLKKTREQVLEQ
HHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCEEEEEHHHHHHHHHHHH
AVAMVKYAKERFPIVQWSAEDACRTDLPFLAEIVSEVIAAGADVVNLPDTVGYLAPAEYG
HHHHHHHHHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHCCCCEECCCHHHCCCCCHHHH
NIFRYIKENARNADKVQLSAHCHDDLGMAVANSLAAIENGADQVESVINGIGERAGNASL
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCH
EEIAVALHIRKDFYQAESTIQLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCC
DGFLKEKTTYEIISPELVGVKTDALVLGKHSGRHAFKDKLQNLGFQLGEEEINKFFNIFK
CCCCCCCCCCEECCCCCCCEECCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
ELTGKKKEFTDEDLISLILEEKTADRKIGYDFISLQVHYGTSQVPTATVSLKDQETDQVI
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHHH
QEAATGAGSVEAVYNTLKRCMDKEVQLLDYRIQSNRKGQDAFAQVYVRVMIDGKESGGRG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCCH
VAQDVLEASAKAYLNAVNRYLVLKTNTEGLSKQAAVGS
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAQQLEKLGVNIMEAGFPASSPGDFNAVKEI
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHH
ARTIKNCSVTGLARSVKGDIDSAWEALKDGAEPRLHIFIATSDIHLKYKLKKTREQVLEQ
HHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCEEEEEHHHHHHHHHHHH
AVAMVKYAKERFPIVQWSAEDACRTDLPFLAEIVSEVIAAGADVVNLPDTVGYLAPAEYG
HHHHHHHHHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHCCCCEECCCHHHCCCCCHHHH
NIFRYIKENARNADKVQLSAHCHDDLGMAVANSLAAIENGADQVESVINGIGERAGNASL
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCH
EEIAVALHIRKDFYQAESTIQLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCC
DGFLKEKTTYEIISPELVGVKTDALVLGKHSGRHAFKDKLQNLGFQLGEEEINKFFNIFK
CCCCCCCCCCEECCCCCCCEECCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
ELTGKKKEFTDEDLISLILEEKTADRKIGYDFISLQVHYGTSQVPTATVSLKDQETDQVI
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHHH
QEAATGAGSVEAVYNTLKRCMDKEVQLLDYRIQSNRKGQDAFAQVYVRVMIDGKESGGRG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCCH
VAQDVLEASAKAYLNAVNRYLVLKTNTEGLSKQAAVGS
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA