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The map label for this gene is leuB [H]

Identifier: 52786689

GI number: 52786689

Start: 2844816

End: 2845937

Strand: Reverse

Name: leuB [H]

Synonym: BLi02957

Alternate gene names: 52786689

Gene position: 2845937-2844816 (Counterclockwise)

Preceding gene: 52786690

Following gene: 52786688

Centisome position: 67.4

GC content: 51.34

Gene sequence:

>1122_bases
TTGTTGAATTTGAAAAAACGAATCGCACTTTTGCCCGGTGACGGAATTGGTCCCGAAGTATTGGACGCGGCTGTTGATGT
GCTGAAAAGCGTTGCTGAACATTATCAGCATGAATTCGAATTTGAATACGGCCTGATCGGCGGGGCGGCAATTGATGAGG
CAGGAGCGCCGCTTCCTGAGGAAACTGTCGCCGCCTGCAAAGCTGCGGACGCCATTTTGCTCGGAGCTGTAGGCGGGCCG
AAATGGGATCAAAATCCTTCTGAGCTGCGGCCTGAAAAAGGGCTGCTCGCCATCCGCAAGCAGCTCGATCTTTTTGCCAA
TCTGCGGCCTGTTAAAGTATTTGAAAGCCTTGCGGGAGCTTCTCCGTTAAAAACCGAATATATCGAAGGTGTTGACTTTG
TCATCGTTCGCGAGCTGACGGGCGGCTTGTATTTTGGAAAGCCGAGCGAGCAGTACGTCAATCAGAACGGCGAGGAAGAA
GCGGTTGACACGCTGTTTTATAAAAAGTCGGAAATGGAAAGGGTCATCAGGGAAGCCTTCCAAATGGCGCAGAGTCGAAA
AGGCAAAGTGACCTCCGTTGATAAAGCCAACGTCCTTGAGTCAAGCAAACTATGGCGCAAGACGGCGGAAGAAGTGGCGA
AAGAGTTTCCTGACGTCAAGCTTGAGCACATGCTCGTCGACAATGCAGCCATGCAGCTCATCTATGCGCCTGGACAGTTT
GATATTATCGTGACAGAAAACATGTTCGGCGATATTTTAAGCGATGAAGCATCGATGCTGACCGGATCGCTCGGCATGCT
GCCGTCGGCCAGCTTATCAAGCTCTGGACTTCATTTATACGAACCTGTGCACGGCTCGGCTCCTGATATCGCGGGACAGA
ACATTGCCAATCCGCTTGCGGCGATCTTGTCCGCGGCTATGATGCTCAGGACGTCATTCGGTCTTGAAGCTGAAGCGCAA
GCTGTTGAACATGCAGTCGATCAAGTTCTCCGGGCAGGAAAACGGACGAAGGACCTGGCAAAAGGCTCGGAACATTGCAC
AACTCAATCGATTACAGGGGAAGTAAAAGCCGCGCTGGCAGACGACAATGCCATTTCAAACATCATGACCGCTTACGTTT
AA

Upstream 100 bases:

>100_bases
CTACTTGAATGCTGTGAACCGCTATCTCGTATTGAAGACGAATACGGAAGGATTGTCAAAACAGGCGGCTGTAGGATCAT
AACAGTAAGAAGGAAGGAGT

Downstream 100 bases:

>100_bases
GGAAAATGGATATTGGTAGAGGGAGGAAGAAAGATGATGCCTCGAACGATCATCGAAAAAATTTGGGATCAACACGTGAT
TAAAAAAGGTGAAGGGAAGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLNLKKRIALLPGDGIGPEVLDAAVDVLKSVAEHYQHEFEFEYGLIGGAAIDEAGAPLPEETVAACKAADAILLGAVGGP
KWDQNPSELRPEKGLLAIRKQLDLFANLRPVKVFESLAGASPLKTEYIEGVDFVIVRELTGGLYFGKPSEQYVNQNGEEE
AVDTLFYKKSEMERVIREAFQMAQSRKGKVTSVDKANVLESSKLWRKTAEEVAKEFPDVKLEHMLVDNAAMQLIYAPGQF
DIIVTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLYEPVHGSAPDIAGQNIANPLAAILSAAMMLRTSFGLEAEAQ
AVEHAVDQVLRAGKRTKDLAKGSEHCTTQSITGEVKAALADDNAISNIMTAYV

Sequences:

>Translated_373_residues
MLNLKKRIALLPGDGIGPEVLDAAVDVLKSVAEHYQHEFEFEYGLIGGAAIDEAGAPLPEETVAACKAADAILLGAVGGP
KWDQNPSELRPEKGLLAIRKQLDLFANLRPVKVFESLAGASPLKTEYIEGVDFVIVRELTGGLYFGKPSEQYVNQNGEEE
AVDTLFYKKSEMERVIREAFQMAQSRKGKVTSVDKANVLESSKLWRKTAEEVAKEFPDVKLEHMLVDNAAMQLIYAPGQF
DIIVTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLYEPVHGSAPDIAGQNIANPLAAILSAAMMLRTSFGLEAEAQ
AVEHAVDQVLRAGKRTKDLAKGSEHCTTQSITGEVKAALADDNAISNIMTAYV
>Mature_373_residues
MLNLKKRIALLPGDGIGPEVLDAAVDVLKSVAEHYQHEFEFEYGLIGGAAIDEAGAPLPEETVAACKAADAILLGAVGGP
KWDQNPSELRPEKGLLAIRKQLDLFANLRPVKVFESLAGASPLKTEYIEGVDFVIVRELTGGLYFGKPSEQYVNQNGEEE
AVDTLFYKKSEMERVIREAFQMAQSRKGKVTSVDKANVLESSKLWRKTAEEVAKEFPDVKLEHMLVDNAAMQLIYAPGQF
DIIVTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLYEPVHGSAPDIAGQNIANPLAAILSAAMMLRTSFGLEAEAQ
AVEHAVDQVLRAGKRTKDLAKGSEHCTTQSITGEVKAALADDNAISNIMTAYV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=358, Percent_Identity=34.3575418994413, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI28178816, Length=363, Percent_Identity=31.404958677686, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=32.5878594249201, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4758582, Length=329, Percent_Identity=31.306990881459, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=31.6109422492401, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28178819, Length=174, Percent_Identity=34.4827586206897, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI87081683, Length=351, Percent_Identity=51.8518518518518, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788101, Length=344, Percent_Identity=36.9186046511628, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787381, Length=383, Percent_Identity=26.6318537859008, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17550882, Length=345, Percent_Identity=32.7536231884058, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71986051, Length=366, Percent_Identity=32.7868852459016, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25144293, Length=366, Percent_Identity=31.6939890710383, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=328, Percent_Identity=27.7439024390244, Blast_Score=121, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=50.6775067750677, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6322097, Length=367, Percent_Identity=36.5122615803815, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=33.4269662921348, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=360, Percent_Identity=32.7777777777778, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24643270, Length=358, Percent_Identity=34.6368715083799, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24643268, Length=358, Percent_Identity=34.6368715083799, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=32.7683615819209, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=32.7683615819209, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24661184, Length=349, Percent_Identity=31.2320916905444, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI161078635, Length=343, Percent_Identity=27.9883381924198, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078633, Length=343, Percent_Identity=27.6967930029155, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650122, Length=343, Percent_Identity=27.6967930029155, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078637, Length=343, Percent_Identity=27.9883381924198, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078639, Length=338, Percent_Identity=28.4023668639053, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20130355, Length=345, Percent_Identity=24.3478260869565, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 40191; Mature: 40191

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLKKRIALLPGDGIGPEVLDAAVDVLKSVAEHYQHEFEFEYGLIGGAAIDEAGAPLPE
CCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHCCCCCCCH
ETVAACKAADAILLGAVGGPKWDQNPSELRPEKGLLAIRKQLDLFANLRPVKVFESLAGA
HHHHHHHHHCCEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
SPLKTEYIEGVDFVIVRELTGGLYFGKPSEQYVNQNGEEEAVDTLFYKKSEMERVIREAF
CCCHHHHHCCCHHEEEEEHHCCEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QMAQSRKGKVTSVDKANVLESSKLWRKTAEEVAKEFPDVKLEHMLVDNAAMQLIYAPGQF
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCE
DIIVTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLYEPVHGSAPDIAGQNIANPLA
EEEEECHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
AILSAAMMLRTSFGLEAEAQAVEHAVDQVLRAGKRTKDLAKGSEHCTTQSITGEVKAALA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCHHHHHHHHHHHHC
DDNAISNIMTAYV
CCHHHHHHHHHCC
>Mature Secondary Structure
MLNLKKRIALLPGDGIGPEVLDAAVDVLKSVAEHYQHEFEFEYGLIGGAAIDEAGAPLPE
CCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHCCCCCCCH
ETVAACKAADAILLGAVGGPKWDQNPSELRPEKGLLAIRKQLDLFANLRPVKVFESLAGA
HHHHHHHHHCCEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
SPLKTEYIEGVDFVIVRELTGGLYFGKPSEQYVNQNGEEEAVDTLFYKKSEMERVIREAF
CCCHHHHHCCCHHEEEEEHHCCEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QMAQSRKGKVTSVDKANVLESSKLWRKTAEEVAKEFPDVKLEHMLVDNAAMQLIYAPGQF
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCE
DIIVTENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLYEPVHGSAPDIAGQNIANPLA
EEEEECHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
AILSAAMMLRTSFGLEAEAQAVEHAVDQVLRAGKRTKDLAKGSEHCTTQSITGEVKAALA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHCCHHHHHHHHHHHHC
DDNAISNIMTAYV
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA