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The map label for this gene is leuC

Identifier: 52786688

GI number: 52786688

Start: 2843364

End: 2844782

Strand: Reverse

Name: leuC

Synonym: BLi02956

Alternate gene names: 52786688

Gene position: 2844782-2843364 (Counterclockwise)

Preceding gene: 52786689

Following gene: 52786687

Centisome position: 67.37

GC content: 50.46

Gene sequence:

>1419_bases
ATGATGCCTCGAACGATCATCGAAAAAATTTGGGATCAACACGTGATTAAAAAAGGTGAAGGGAAGCCTGACCTTCTCTA
TATCGATCTGCATCTGATACATGAGGTAACGTCGCCTCAGGCCTTTGAAGGCCTGAGGCAAAAGAACAGAAAAGTCAGAC
GGCCTCAAAATACGTTTGCCACGATGGACCACAACATTCCGACTGTCAATCGGTTTGAGATTAAAGATGATGTCGCTAAA
CGGCAGGTCAGTGCGCTCGAACGGAATTGCGAAGAATTCGGCATCCGCCTTGCAGACTTGTCAAGCGTAGACCAGGGGAT
CGTCCATGTCATCGGGCCTGAACTGGGATTGACGCTCCCGGGCAAAACGATCGTTTGCGGAGACAGCCATACATCCACAC
ACGGAGCGTTTGGCGCGCTCGCTTTCGGAATCGGAACGAGCGAAGTCGAACACGTCTTGTCAACGCAGACGCTTTGGCAG
CAAAAACCGAAGACGCTGGAAATCCGCGTTGACGGCAAACTGCAAAAAGGAGTGACGGCTAAAGACGTCATTCTGGCGAT
CATCGGAAAATACGGGGTTAAATTCGGTGCGGGCTATGTCATTGAATATACCGGCGAAGTGTTCCGAAACATGTCGATGG
ACGAACGGATGACGGTTTGCAACATGTCAATCGAAGCTGGGGCAAGAGCTGGATTGATTGCACCGGATGAGACGACGTTT
GAATATGTCAAAACAAAAAAATATGCGCCAAAAGGCGAGGATTTTGAGAAAGCTGTAGAAGAATGGAAGAACCTGAAAAC
CGATCCGGGGGCGGTCTACGACCGGACGATCGTGATGGACGGAAGCGCCATTTCTCCGATGGTGACGTGGGGCATCAACC
CTGGCATGGTGCTGCCGGTTGATGAAACGATTCCGGGGCCTGAGGCTTTCTTGCAGGAAGACGATAAAAAAGAAGCGCAG
CGCGCATATGAATATATGGGTCTTGAACCATATAAAAAAATCGAGGACATTACAATTGAGCATGTCTTTATCGGTTCATG
CACCAACTCGCGTATGACAGATCTTCGCCAGGCGGCCGAAATGATTAAAGGTCAAAAAGTCGCCGACGGCGTCAGAGCGA
TCGTCGTACCCGGCTCGCAAAGCGTCAAAATCCAAGCGGAAAAAGAAGGGCTTCACAAGATTTTCATCGAAGCGGGCTTT
GAATGGAGAGAATCGGGATGCAGCATGTGTTTGAGCATGAACAATGATGTTGTCCCTGAAGGAGAGCGCTGCGCTTCGAC
TTCCAACCGCAATTTTGAAGGCAGGCAGGGCAAAGGAGCCAGAACGCATCTCGTCAGCCCGGCGATGGCTGCGATGGCCG
CGATTCACGGCCGCTTTGTCGATGTCAGAAAATATTACCAAGAGACAACAGCTGTATAG

Upstream 100 bases:

>100_bases
AGGGGAAGTAAAAGCCGCGCTGGCAGACGACAATGCCATTTCAAACATCATGACCGCTTACGTTTAAGGAAAATGGATAT
TGGTAGAGGGAGGAAGAAAG

Downstream 100 bases:

>100_bases
AGGAGTGACAAGATGGAGCCATTAAAGACCCATAACGGAATAGCGGCGGTGCTAAACAGAATCAACGTAGACACGGACCA
GATCATTCCGAAGCAATTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MMPRTIIEKIWDQHVIKKGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKNRKVRRPQNTFATMDHNIPTVNRFEIKDDVAK
RQVSALERNCEEFGIRLADLSSVDQGIVHVIGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QKPKTLEIRVDGKLQKGVTAKDVILAIIGKYGVKFGAGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDETTF
EYVKTKKYAPKGEDFEKAVEEWKNLKTDPGAVYDRTIVMDGSAISPMVTWGINPGMVLPVDETIPGPEAFLQEDDKKEAQ
RAYEYMGLEPYKKIEDITIEHVFIGSCTNSRMTDLRQAAEMIKGQKVADGVRAIVVPGSQSVKIQAEKEGLHKIFIEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGRFVDVRKYYQETTAV

Sequences:

>Translated_472_residues
MMPRTIIEKIWDQHVIKKGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKNRKVRRPQNTFATMDHNIPTVNRFEIKDDVAK
RQVSALERNCEEFGIRLADLSSVDQGIVHVIGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QKPKTLEIRVDGKLQKGVTAKDVILAIIGKYGVKFGAGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDETTF
EYVKTKKYAPKGEDFEKAVEEWKNLKTDPGAVYDRTIVMDGSAISPMVTWGINPGMVLPVDETIPGPEAFLQEDDKKEAQ
RAYEYMGLEPYKKIEDITIEHVFIGSCTNSRMTDLRQAAEMIKGQKVADGVRAIVVPGSQSVKIQAEKEGLHKIFIEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGRFVDVRKYYQETTAV
>Mature_472_residues
MMPRTIIEKIWDQHVIKKGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKNRKVRRPQNTFATMDHNIPTVNRFEIKDDVAK
RQVSALERNCEEFGIRLADLSSVDQGIVHVIGPELGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQ
QKPKTLEIRVDGKLQKGVTAKDVILAIIGKYGVKFGAGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDETTF
EYVKTKKYAPKGEDFEKAVEEWKNLKTDPGAVYDRTIVMDGSAISPMVTWGINPGMVLPVDETIPGPEAFLQEDDKKEAQ
RAYEYMGLEPYKKIEDITIEHVFIGSCTNSRMTDLRQAAEMIKGQKVADGVRAIVVPGSQSVKIQAEKEGLHKIFIEAGF
EWRESGCSMCLSMNNDVVPEGERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGRFVDVRKYYQETTAV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=368, Percent_Identity=28.804347826087, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI41352693, Length=462, Percent_Identity=25.1082251082251, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=60.9913793103448, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI87081781, Length=342, Percent_Identity=27.4853801169591, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=25.6130790190736, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI2367097, Length=349, Percent_Identity=23.7822349570201, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=379, Percent_Identity=30.0791556728232, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=379, Percent_Identity=30.0791556728232, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=325, Percent_Identity=29.8461538461538, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=456, Percent_Identity=25.6578947368421, Blast_Score=105, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=56.9002123142251, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6320440, Length=456, Percent_Identity=26.9736842105263, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6322261, Length=384, Percent_Identity=29.9479166666667, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=27.6243093922652, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=27.2264631043257, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=27.2264631043257, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161076999, Length=393, Percent_Identity=27.2264631043257, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28571643, Length=392, Percent_Identity=26.0204081632653, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24645686, Length=456, Percent_Identity=26.0964912280702, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17137564, Length=461, Percent_Identity=24.7288503253796, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_BACLD (Q65GJ0)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080100.1
- RefSeq:   YP_092517.1
- ProteinModelPortal:   Q65GJ0
- STRING:   Q65GJ0
- EnsemblBacteria:   EBBACT00000054208
- EnsemblBacteria:   EBBACT00000061323
- GeneID:   3028056
- GeneID:   3098435
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02956
- KEGG:   bli:BL00613
- NMPDR:   fig|279010.5.peg.3306
- eggNOG:   COG0065
- GeneTree:   EBGT00050000000860
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   BLIC279010-1:BLI02956-MONOMER
- BioCyc:   BLIC279010:BL00613-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 52415; Mature: 52415

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRTIIEKIWDQHVIKKGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKNRKVRRPQNTFA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHE
TMDHNIPTVNRFEIKDDVAKRQVSALERNCEEFGIRLADLSSVDQGIVHVIGPELGLTLP
EECCCCCCCEEEECHHHHHHHHHHHHHHCHHHHCCEEEHHHHHHCCCEEEECCCCCCCCC
GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQKPKTLEIRVDGKLQKGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCCCCH
KDVILAIIGKYGVKFGAGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDETTF
HHHHHHHHHHHCHHHCCCEEEEECHHHHHCCCCHHHHEEEECCHHCCCCCCCCCCCCCHH
EYVKTKKYAPKGEDFEKAVEEWKNLKTDPGAVYDRTIVMDGSAISPMVTWGINPGMVLPV
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEC
DETIPGPEAFLQEDDKKEAQRAYEYMGLEPYKKIEDITIEHVFIGSCTNSRMTDLRQAAE
CCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHEEEEEEEEECCCCHHHHHHHHHHH
MIKGQKVADGVRAIVVPGSQSVKIQAEKEGLHKIFIEAGFEWRESGCSMCLSMNNDVVPE
HHCCCHHHCCCEEEEECCCCCEEEEECCCCHHEEHHHCCCHHHHCCCCEEEECCCCCCCC
GERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGRFVDVRKYYQETTAV
CHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMPRTIIEKIWDQHVIKKGEGKPDLLYIDLHLIHEVTSPQAFEGLRQKNRKVRRPQNTFA
CCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHE
TMDHNIPTVNRFEIKDDVAKRQVSALERNCEEFGIRLADLSSVDQGIVHVIGPELGLTLP
EECCCCCCCEEEECHHHHHHHHHHHHHHCHHHHCCEEEHHHHHHCCCEEEECCCCCCCCC
GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQKPKTLEIRVDGKLQKGVTA
CCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCCCCH
KDVILAIIGKYGVKFGAGYVIEYTGEVFRNMSMDERMTVCNMSIEAGARAGLIAPDETTF
HHHHHHHHHHHCHHHCCCEEEEECHHHHHCCCCHHHHEEEECCHHCCCCCCCCCCCCCHH
EYVKTKKYAPKGEDFEKAVEEWKNLKTDPGAVYDRTIVMDGSAISPMVTWGINPGMVLPV
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEC
DETIPGPEAFLQEDDKKEAQRAYEYMGLEPYKKIEDITIEHVFIGSCTNSRMTDLRQAAE
CCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHEEEEEEEEECCCCHHHHHHHHHHH
MIKGQKVADGVRAIVVPGSQSVKIQAEKEGLHKIFIEAGFEWRESGCSMCLSMNNDVVPE
HHCCCHHHCCCEEEEECCCCCEEEEECCCCHHEEHHHCCCHHHHCCCCEEEECCCCCCCC
GERCASTSNRNFEGRQGKGARTHLVSPAMAAMAAIHGRFVDVRKYYQETTAV
CHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA