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The map label for this gene is leuD

Identifier: 52786687

GI number: 52786687

Start: 2842752

End: 2843351

Strand: Reverse

Name: leuD

Synonym: BLi02955

Alternate gene names: 52786687

Gene position: 2843351-2842752 (Counterclockwise)

Preceding gene: 52786688

Following gene: 52786686

Centisome position: 67.34

GC content: 46.67

Gene sequence:

>600_bases
ATGGAGCCATTAAAGACCCATAACGGAATAGCGGCGGTGCTAAACAGAATCAACGTAGACACGGACCAGATCATTCCGAA
GCAATTTTTAAAAAGGATCGAAAGAACGGGATACGGCCGTTTTGCTTTCTTTGACTGGCGCTATCTGGATAACGGCGATC
CCAATCCTGATTTTGAACTGAACCGGCCGGAATACAAAGGCGCTTCGATTTTAATCGCCGGCGAAAACTTCGGCTGCGGC
TCTTCAAGGGAACACGCGCCATGGGCGCTTGACGACTACGGATTTAAAATCATTATCGCGCCGTCTTTTGCCGATATCTT
TCACCAAAACTGTTTTAAAAACGGCATGCTGCCGATCCGCCTTCCTTATGAGGCGTGGAAAGAGCTTGCTGAACAGTATG
AGTATCAATCTTTGACGATGACGGTCGATCTCGAAAAACAGACGATAACAGATCATGCCGGGCGGCAGATTGCCTTTGAG
GTCGATCCGCATTGGAAAGAGATGCTGCTGAACGGTTACGACGAAATTTCTTTAACATTGCTCCTTGAAGAAGAAATCGA
ACAATTTGAGAAACAGAGAAGTTCTTGGCTGCAAGCATAA

Upstream 100 bases:

>100_bases
CGTCAGCCCGGCGATGGCTGCGATGGCCGCGATTCACGGCCGCTTTGTCGATGTCAGAAAATATTACCAAGAGACAACAG
CTGTATAGAGGAGTGACAAG

Downstream 100 bases:

>100_bases
AAAAGCTGTCCGATGTAGATCGGAGGCTTTTTTTGCTGGCGGCAGCATACTGCCGCCGGTTCTTTCTTGTGTTTAGCGGG
CCGGGTTGATACACTTGTTA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MEPLKTHNGIAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLDNGDPNPDFELNRPEYKGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRLPYEAWKELAEQYEYQSLTMTVDLEKQTITDHAGRQIAFE
VDPHWKEMLLNGYDEISLTLLLEEEIEQFEKQRSSWLQA

Sequences:

>Translated_199_residues
MEPLKTHNGIAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLDNGDPNPDFELNRPEYKGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRLPYEAWKELAEQYEYQSLTMTVDLEKQTITDHAGRQIAFE
VDPHWKEMLLNGYDEISLTLLLEEEIEQFEKQRSSWLQA
>Mature_199_residues
MEPLKTHNGIAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLDNGDPNPDFELNRPEYKGASILIAGENFGCG
SSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIRLPYEAWKELAEQYEYQSLTMTVDLEKQTITDHAGRQIAFE
VDPHWKEMLLNGYDEISLTLLLEEEIEQFEKQRSSWLQA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=200, Percent_Identity=49, Blast_Score=189, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=199, Percent_Identity=48.7437185929648, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_BACLD (Q65GJ1)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080099.1
- RefSeq:   YP_092516.1
- ProteinModelPortal:   Q65GJ1
- SMR:   Q65GJ1
- STRING:   Q65GJ1
- EnsemblBacteria:   EBBACT00000057041
- EnsemblBacteria:   EBBACT00000059588
- GeneID:   3027951
- GeneID:   3098434
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02955
- KEGG:   bli:BL00615
- NMPDR:   fig|279010.5.peg.3305
- eggNOG:   COG0066
- GeneTree:   EBGT00050000002923
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   BLIC279010-1:BLI02955-MONOMER
- BioCyc:   BLIC279010:BL00615-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23166; Mature: 23166

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLKTHNGIAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLDNGDPNPDFEL
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
NRPEYKGASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIR
CCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEE
LPYEAWKELAEQYEYQSLTMTVDLEKQTITDHAGRQIAFEVDPHWKEMLLNGYDEISLTL
ECHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCEEEEEECCCHHHHHHCCCCCEEEEE
LLEEEIEQFEKQRSSWLQA
EEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPLKTHNGIAAVLNRINVDTDQIIPKQFLKRIERTGYGRFAFFDWRYLDNGDPNPDFEL
CCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCC
NRPEYKGASILIAGENFGCGSSREHAPWALDDYGFKIIIAPSFADIFHQNCFKNGMLPIR
CCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCEEEE
LPYEAWKELAEQYEYQSLTMTVDLEKQTITDHAGRQIAFEVDPHWKEMLLNGYDEISLTL
ECHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCEEEEEECCCHHHHHHCCCCCEEEEE
LLEEEIEQFEKQRSSWLQA
EEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA