Click here to switch to the map view.

The map label for this gene is hemB [H]

Identifier: 52786675

GI number: 52786675

Start: 2827955

End: 2828929

Strand: Reverse

Name: hemB [H]

Synonym: BLi02943

Alternate gene names: 52786675

Gene position: 2828929-2827955 (Counterclockwise)

Preceding gene: 52786676

Following gene: 52786674

Centisome position: 66.99

GC content: 49.85

Gene sequence:

>975_bases
ATGGAAGCATCATTTCAAAGACACCGCCGCCTGCGGACGACAGCAGGTATGCGGAAATTGGTAAGGGAAACCGAGTTAAG
AGCATCTGATTTCATTTATCCGATATTTGTTACCGAGGAAGAGAACGTCAAAAGCGAAGTTCCTTCTATGCCGGGCGTGT
ACCAGCTGTCTTTGGACCGTTTGAAAGAGGAGATGGAGGAAGTCGCAAGCCTCGGCATTCAATCTGTGATCGTCTTCGGC
GTGCCTGAGCACAAAGATGATGTGGGAAGCGGAGCCTATCACGATCACGGCATCGTCCAAAAAGCGATTGCACAAATTAA
ACAGGACTTTCCTGAACTTGTCGTTATCGCCGATACGTGCCTATGCGAGTACACGGACCACGGTCACTGCGGCCTGGTGG
AAAACGGTGAAATTTTAAATGACGAATCGCTTGTTCTTTTGGCAAAAACCGCTGTCAGCCAAGCCCGTGCAGGTGCTGAC
ATTATTGCGCCGTCAAACATGATGGACGGTTTCGTTGCCGCTATCAGGGAAGCGCTTGATCAGGAAGGATTTACAAATGT
GCCGATCATGTCGTACGCTGTAAAATATGCGAGCGCTTTTTACGGACCGTTCCGCGATGCTGCGGGAAGCAGTCCGCAGT
TTGGCGACCGCAAGACGTACCAAATGGATTATGCCAACCGCAGGGAAGCTCTCCGGGAGGCAAAATCCGATGTGGAAGAA
GGCGCCGATGTTCTGATCGTCAAGCCGACGCTTTCATATCTTGATATCGTCAGAGAAGTGAAGAACGAATTTAACCTGCC
TGTTGTCGGATACAATGTGAGCGGGGAGTATGCTATGGTCAAAGCCGCCGCCCAAAACGGATGGATTGAAGAAAAAGCTC
TTGTTCTCGAAATGCTGACAAGCATGAAGCGGGCTGGCGCCGATTTGATCATTACGTACTTTGCAAAAGACGCGGCAAAA
TGGATTTCGGAATAA

Upstream 100 bases:

>100_bases
GCTGTCGGACTACGGAATTCCATCATATATGCCGGACGTCTATACAATCGACGGAATGCTGCGCGAAATGTGCCGCATTT
CAAGAAAGGGAGAATGACTA

Downstream 100 bases:

>100_bases
GAATTTGAACTGGGGGTTTTAGAGGATGCGGAGCTATGAGAAGTCTAAAGCGGCATTCGAAGAGGCGCAACGAGTCATGC
CGGGCGGGGTGAACAGCCCG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEASFQRHRRLRTTAGMRKLVRETELRASDFIYPIFVTEEENVKSEVPSMPGVYQLSLDRLKEEMEEVASLGIQSVIVFG
VPEHKDDVGSGAYHDHGIVQKAIAQIKQDFPELVVIADTCLCEYTDHGHCGLVENGEILNDESLVLLAKTAVSQARAGAD
IIAPSNMMDGFVAAIREALDQEGFTNVPIMSYAVKYASAFYGPFRDAAGSSPQFGDRKTYQMDYANRREALREAKSDVEE
GADVLIVKPTLSYLDIVREVKNEFNLPVVGYNVSGEYAMVKAAAQNGWIEEKALVLEMLTSMKRAGADLIITYFAKDAAK
WISE

Sequences:

>Translated_324_residues
MEASFQRHRRLRTTAGMRKLVRETELRASDFIYPIFVTEEENVKSEVPSMPGVYQLSLDRLKEEMEEVASLGIQSVIVFG
VPEHKDDVGSGAYHDHGIVQKAIAQIKQDFPELVVIADTCLCEYTDHGHCGLVENGEILNDESLVLLAKTAVSQARAGAD
IIAPSNMMDGFVAAIREALDQEGFTNVPIMSYAVKYASAFYGPFRDAAGSSPQFGDRKTYQMDYANRREALREAKSDVEE
GADVLIVKPTLSYLDIVREVKNEFNLPVVGYNVSGEYAMVKAAAQNGWIEEKALVLEMLTSMKRAGADLIITYFAKDAAK
WISE
>Mature_324_residues
MEASFQRHRRLRTTAGMRKLVRETELRASDFIYPIFVTEEENVKSEVPSMPGVYQLSLDRLKEEMEEVASLGIQSVIVFG
VPEHKDDVGSGAYHDHGIVQKAIAQIKQDFPELVVIADTCLCEYTDHGHCGLVENGEILNDESLVLLAKTAVSQARAGAD
IIAPSNMMDGFVAAIREALDQEGFTNVPIMSYAVKYASAFYGPFRDAAGSSPQFGDRKTYQMDYANRREALREAKSDVEE
GADVLIVKPTLSYLDIVREVKNEFNLPVVGYNVSGEYAMVKAAAQNGWIEEKALVLEMLTSMKRAGADLIITYFAKDAAK
WISE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=49.0196078431373, Blast_Score=290, Evalue=9e-79,
Organism=Escherichia coli, GI87081728, Length=316, Percent_Identity=51.8987341772152, Blast_Score=315, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=42.0382165605096, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21358291, Length=304, Percent_Identity=41.4473684210526, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35934; Mature: 35934

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEASFQRHRRLRTTAGMRKLVRETELRASDFIYPIFVTEEENVKSEVPSMPGVYQLSLDR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHCCCCCCCHHHHHHH
LKEEMEEVASLGIQSVIVFGVPEHKDDVGSGAYHDHGIVQKAIAQIKQDFPELVVIADTC
HHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEEHHHH
LCEYTDHGHCGLVENGEILNDESLVLLAKTAVSQARAGADIIAPSNMMDGFVAAIREALD
HHCCCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHH
QEGFTNVPIMSYAVKYASAFYGPFRDAAGSSPQFGDRKTYQMDYANRREALREAKSDVEE
CCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHC
GADVLIVKPTLSYLDIVREVKNEFNLPVVGYNVSGEYAMVKAAAQNGWIEEKALVLEMLT
CCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCHHEEEEHHHCCCHHHHHHHHHHHH
SMKRAGADLIITYFAKDAAKWISE
HHHHCCCCEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MEASFQRHRRLRTTAGMRKLVRETELRASDFIYPIFVTEEENVKSEVPSMPGVYQLSLDR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHCCCCCCCHHHHHHH
LKEEMEEVASLGIQSVIVFGVPEHKDDVGSGAYHDHGIVQKAIAQIKQDFPELVVIADTC
HHHHHHHHHHCCHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEEHHHH
LCEYTDHGHCGLVENGEILNDESLVLLAKTAVSQARAGADIIAPSNMMDGFVAAIREALD
HHCCCCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHH
QEGFTNVPIMSYAVKYASAFYGPFRDAAGSSPQFGDRKTYQMDYANRREALREAKSDVEE
CCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHC
GADVLIVKPTLSYLDIVREVKNEFNLPVVGYNVSGEYAMVKAAAQNGWIEEKALVLEMLT
CCCEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCHHEEEEHHHCCCHHHHHHHHHHHH
SMKRAGADLIITYFAKDAAKWISE
HHHHCCCCEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377 [H]