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The map label for this gene is hemL

Identifier: 52786674

GI number: 52786674

Start: 2826634

End: 2827929

Strand: Reverse

Name: hemL

Synonym: BLi02942

Alternate gene names: 52786674

Gene position: 2827929-2826634 (Counterclockwise)

Preceding gene: 52786675

Following gene: 52786673

Centisome position: 66.97

GC content: 51.93

Gene sequence:

>1296_bases
ATGCGGAGCTATGAGAAGTCTAAAGCGGCATTCGAAGAGGCGCAACGAGTCATGCCGGGCGGGGTGAACAGCCCGGTCAG
AGCCTTTAAATCAGTCAACATGGACCCGATTTTTATGGAGCGGGGAAAAGGATCAAAAATCTATGATATTGACGGAAACG
AATACATCGATTATGTCCTGTCATGGGGACCGCTTATTTTGGGCCACAGCAATGAAAAAGTGGTGAAAGAGATTCAAAAG
GCGGCAGAACACGGGACAAGCTTCGGTGCGCCGACAGAACTGGAAACCGAGCTTGCCGAGCTTGTCATCGACCGCGTTCC
TTCCATAGAAATCGTCCGGATGGTGAGCTCCGGTACGGAGGCGACGATGAGCGCCTTGCGGCTGGCGCGCGGATACACAG
GCCGTAACAAAATCGTAAAATTTGAAGGCTGCTACCATGGACACGGTGATTCCCTGCTGATTAAAGCGGGCTCGGGAGTA
GCGACACTCGGCCTCCCTGACAGCCCCGGAGTTCCGGAAAGCATTGCAAAAAACACGATTACCGTTCCTTACAATGATCT
GGAAAGCATGAAACTTGTCTTTCAAGAATTCGGAGATGACATTGCAGGCGTCATCGTTGAACCTGTCGCTGGCAATATGG
GCGTCGTACCGCCGGTTAAAGGGTTCCTCGAAGGGCTTCGCGAGCTGACTGAGACGCATGGCGCCCTGCTGATATTTGAT
GAAGTGATGACAGGATTCAGGGTGGATTACAACTGTGCCCAAGGCTATTTCGGGGTGACGCCTGATTTGACATGCCTCGG
AAAAGTGATCGGCGGCGGTCTTCCTGTCGGCGCTTATGGAGGAAAGGCGGAAATCATGGAGAAGATCGCGCCGAGCGGTC
CGATCTATCAGGCCGGGACGCTGTCCGGCAACCCGCTTGCCATGACAGCCGGACTTGAAACGTTGAAACAGCTGACCCCT
GAATCCTACCGGGAATTCTCCCGCAAAGCTGACCGCTTGGAAAAAGGAATTTCTGAAGCTGCAGAGAAAAATGGCATTCC
GTGTACATTCAACAGGGCGGGGTCTATGATCGGCTTTTTCTTCACAAATGGACCGGTCATCAATTATGATACAGCGAAAC
AGTCTGACCTCGGACTGTTTGCCGAATATTATAAAGGAATGGCCGATGAAGGCGTGTTTCTGCCGCCTTCACAGTTTGAA
GGGCTGTTCTTGTCAACCGCTCATACGGATGACGACATTGAGCATACGATTAAAGCTGCTGAACGCGTATTCGAAAGAAT
CAGCCGCTCCAGATAA

Upstream 100 bases:

>100_bases
AGCATGAAGCGGGCTGGCGCCGATTTGATCATTACGTACTTTGCAAAAGACGCGGCAAAATGGATTTCGGAATAAGAATT
TGAACTGGGGGTTTTAGAGG

Downstream 100 bases:

>100_bases
AGAAAAGGGCTGCCGGACATTGCCGGCGGCTCTTTTTTGCATGGTGCCGAAAACGGCTGCTGATTTTTCTGTTCATATTC
TGCCTGCTTTTCACATACAT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MRSYEKSKAAFEEAQRVMPGGVNSPVRAFKSVNMDPIFMERGKGSKIYDIDGNEYIDYVLSWGPLILGHSNEKVVKEIQK
AAEHGTSFGAPTELETELAELVIDRVPSIEIVRMVSSGTEATMSALRLARGYTGRNKIVKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPESIAKNTITVPYNDLESMKLVFQEFGDDIAGVIVEPVAGNMGVVPPVKGFLEGLRELTETHGALLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEKIAPSGPIYQAGTLSGNPLAMTAGLETLKQLTP
ESYREFSRKADRLEKGISEAAEKNGIPCTFNRAGSMIGFFFTNGPVINYDTAKQSDLGLFAEYYKGMADEGVFLPPSQFE
GLFLSTAHTDDDIEHTIKAAERVFERISRSR

Sequences:

>Translated_431_residues
MRSYEKSKAAFEEAQRVMPGGVNSPVRAFKSVNMDPIFMERGKGSKIYDIDGNEYIDYVLSWGPLILGHSNEKVVKEIQK
AAEHGTSFGAPTELETELAELVIDRVPSIEIVRMVSSGTEATMSALRLARGYTGRNKIVKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPESIAKNTITVPYNDLESMKLVFQEFGDDIAGVIVEPVAGNMGVVPPVKGFLEGLRELTETHGALLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEKIAPSGPIYQAGTLSGNPLAMTAGLETLKQLTP
ESYREFSRKADRLEKGISEAAEKNGIPCTFNRAGSMIGFFFTNGPVINYDTAKQSDLGLFAEYYKGMADEGVFLPPSQFE
GLFLSTAHTDDDIEHTIKAAERVFERISRSR
>Mature_431_residues
MRSYEKSKAAFEEAQRVMPGGVNSPVRAFKSVNMDPIFMERGKGSKIYDIDGNEYIDYVLSWGPLILGHSNEKVVKEIQK
AAEHGTSFGAPTELETELAELVIDRVPSIEIVRMVSSGTEATMSALRLARGYTGRNKIVKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPESIAKNTITVPYNDLESMKLVFQEFGDDIAGVIVEPVAGNMGVVPPVKGFLEGLRELTETHGALLIFD
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEKIAPSGPIYQAGTLSGNPLAMTAGLETLKQLTP
ESYREFSRKADRLEKGISEAAEKNGIPCTFNRAGSMIGFFFTNGPVINYDTAKQSDLGLFAEYYKGMADEGVFLPPSQFE
GLFLSTAHTDDDIEHTIKAAERVFERISRSR

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=311, Percent_Identity=31.8327974276527, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI13994255, Length=330, Percent_Identity=26.0606060606061, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI284507298, Length=230, Percent_Identity=34.3478260869565, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI24119277, Length=314, Percent_Identity=27.3885350318471, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=54.9528301886792, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1789016, Length=381, Percent_Identity=30.1837270341207, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1788044, Length=350, Percent_Identity=32.2857142857143, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789759, Length=328, Percent_Identity=30.7926829268293, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI145693181, Length=318, Percent_Identity=31.4465408805031, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787560, Length=351, Percent_Identity=29.3447293447293, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786991, Length=286, Percent_Identity=26.2237762237762, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71992977, Length=344, Percent_Identity=28.1976744186047, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=331, Percent_Identity=28.7009063444109, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25144274, Length=180, Percent_Identity=32.2222222222222, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324432, Length=369, Percent_Identity=27.9132791327913, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323470, Length=302, Percent_Identity=27.1523178807947, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357415, Length=307, Percent_Identity=26.3843648208469, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA2_BACLD (Q65GK4)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_080086.1
- RefSeq:   YP_092503.1
- ProteinModelPortal:   Q65GK4
- SMR:   Q65GK4
- STRING:   Q65GK4
- EnsemblBacteria:   EBBACT00000054352
- EnsemblBacteria:   EBBACT00000062166
- GeneID:   3028404
- GeneID:   3098394
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02942
- KEGG:   bli:BL00628
- NMPDR:   fig|279010.5.peg.3269
- eggNOG:   COG0001
- GeneTree:   EBGT00070000031737
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- ProtClustDB:   PRK00062
- BioCyc:   BLIC279010-1:BLI02942-MONOMER
- BioCyc:   BLIC279010:BL00628-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46641; Mature: 46641

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYEKSKAAFEEAQRVMPGGVNSPVRAFKSVNMDPIFMERGKGSKIYDIDGNEYIDYVL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHH
SWGPLILGHSNEKVVKEIQKAAEHGTSFGAPTELETELAELVIDRVPSIEIVRMVSSGTE
HCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHH
ATMSALRLARGYTGRNKIVKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPESIAKNTI
HHHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEECCCCEEEECCCCCCCCCHHHHCCEE
TVPYNDLESMKLVFQEFGDDIAGVIVEPVAGNMGVVPPVKGFLEGLRELTETHGALLIFD
ECCCCCHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEH
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEKIAPSGPIYQAGT
HHHHCCEECEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGLETLKQLTPESYREFSRKADRLEKGISEAAEKNGIPCTFNRAGSMIGFF
CCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEE
FTNGPVINYDTAKQSDLGLFAEYYKGMADEGVFLPPSQFEGLFLSTAHTDDDIEHTIKAA
ECCCCEEECCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCEEEEECCCCHHHHHHHHHH
ERVFERISRSR
HHHHHHHHCCC
>Mature Secondary Structure
MRSYEKSKAAFEEAQRVMPGGVNSPVRAFKSVNMDPIFMERGKGSKIYDIDGNEYIDYVL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHH
SWGPLILGHSNEKVVKEIQKAAEHGTSFGAPTELETELAELVIDRVPSIEIVRMVSSGTE
HCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHH
ATMSALRLARGYTGRNKIVKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPESIAKNTI
HHHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEECCCCEEEECCCCCCCCCHHHHCCEE
TVPYNDLESMKLVFQEFGDDIAGVIVEPVAGNMGVVPPVKGFLEGLRELTETHGALLIFD
ECCCCCHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEH
EVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKAEIMEKIAPSGPIYQAGT
HHHHCCEECEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCC
LSGNPLAMTAGLETLKQLTPESYREFSRKADRLEKGISEAAEKNGIPCTFNRAGSMIGFF
CCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEE
FTNGPVINYDTAKQSDLGLFAEYYKGMADEGVFLPPSQFEGLFLSTAHTDDDIEHTIKAA
ECCCCEEECCCCCCCCHHHHHHHHCCCCCCCEECCCCCCCCEEEEECCCCHHHHHHHHHH
ERVFERISRSR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA