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The map label for this gene is trpF

Identifier: 52786144

GI number: 52786144

Start: 2344175

End: 2344831

Strand: Reverse

Name: trpF

Synonym: BLi02400

Alternate gene names: 52786144

Gene position: 2344831-2344175 (Counterclockwise)

Preceding gene: 52786145

Following gene: 52786143

Centisome position: 55.53

GC content: 49.77

Gene sequence:

>657_bases
ATGAAGAAACCGAGTCTAAAATATTGCGGGATTCATTCATTGGAAGATTTAAAGGTGACAGCCCGCTCGAAAGCCGATTA
TCTCGGATTTATTTTCGCCGAAAGCAAACGGTCGGTAGACCCGGCGGATGTGAAACGGTGGTGCGGCGAAACGGATACGC
CAGGTAAAAAGCTTGTCGGCGTTTTTGTTAATGAAAACATGAGCCGAATGGCTGAAATCGTAAAAGATGCAGGCTTGGAC
GTGATCCAGCTTCACGGAGATGAAACGGCGGCAGACATCAAGCGGCTTAAAAGCATGACAGACTGCGAGATATGGAAGGC
GCTCCCTCACGGGAATGATACCGTACAGAGCATGGCTTCTTTCGCACCTTACATCGACGGGTATGTCATCGACTCGTCTG
TCAAAGGGATGAGAGGGGGAACGGGAGTTTCGTTTTCGTGGGACAGCGTCCCGCTGTATATAGATGCAGCCCAAAGAGAA
GGCAAGCGCCTGTTTATCGCCGGAGGCGTGAACCCGGACACAATCGCAGATCTTTTAAAGCGGCGTCCGCCGGGAATCGA
CTTGGCAAGCGGAATCGAAGAAAGAGGCAGAAAAAGCGAAAAGCTGATCAGCCTTTTAGAAGAAAGGATGTTCGAACATG
TATTCATATCCGAATGA

Upstream 100 bases:

>100_bases
TAAAAACGTGCGGAGCCCAAGCCGTGCTTGTCGGCGAATCGCTGATGAGAGAGACGTCACAGCTGAAGGCTGTCTATGCG
TTATTCGGAGAGGACGGCTG

Downstream 100 bases:

>100_bases
ACAAGGCAGGTTTGGTGATTTTGGCGGGAAATTCGTCCCGGAAACACTCATGCAGCCTTTGGAAGAAATCGAAAAAGCGT
TTCTTGAACTGAAAGACGAC

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKKPSLKYCGIHSLEDLKVTARSKADYLGFIFAESKRSVDPADVKRWCGETDTPGKKLVGVFVNENMSRMAEIVKDAGLD
VIQLHGDETAADIKRLKSMTDCEIWKALPHGNDTVQSMASFAPYIDGYVIDSSVKGMRGGTGVSFSWDSVPLYIDAAQRE
GKRLFIAGGVNPDTIADLLKRRPPGIDLASGIEERGRKSEKLISLLEERMFEHVFISE

Sequences:

>Translated_218_residues
MKKPSLKYCGIHSLEDLKVTARSKADYLGFIFAESKRSVDPADVKRWCGETDTPGKKLVGVFVNENMSRMAEIVKDAGLD
VIQLHGDETAADIKRLKSMTDCEIWKALPHGNDTVQSMASFAPYIDGYVIDSSVKGMRGGTGVSFSWDSVPLYIDAAQRE
GKRLFIAGGVNPDTIADLLKRRPPGIDLASGIEERGRKSEKLISLLEERMFEHVFISE
>Mature_218_residues
MKKPSLKYCGIHSLEDLKVTARSKADYLGFIFAESKRSVDPADVKRWCGETDTPGKKLVGVFVNENMSRMAEIVKDAGLD
VIQLHGDETAADIKRLKSMTDCEIWKALPHGNDTVQSMASFAPYIDGYVIDSSVKGMRGGTGVSFSWDSVPLYIDAAQRE
GKRLFIAGGVNPDTIADLLKRRPPGIDLASGIEERGRKSEKLISLLEERMFEHVFISE

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=202, Percent_Identity=28.7128712871287, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320210, Length=220, Percent_Identity=31.3636363636364, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_BACLD (Q65I34)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079564.2
- RefSeq:   YP_091973.1
- HSSP:   Q56320
- ProteinModelPortal:   Q65I34
- SMR:   Q65I34
- STRING:   Q65I34
- EnsemblBacteria:   EBBACT00000056449
- EnsemblBacteria:   EBBACT00000059719
- GeneID:   3028761
- GeneID:   3100694
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02400
- KEGG:   bli:BL02772
- NMPDR:   fig|279010.5.peg.250
- eggNOG:   COG0135
- GeneTree:   EBGT00050000002322
- HOGENOM:   HBG554803
- ProtClustDB:   PRK01222
- BioCyc:   BLIC279010-1:BLI02400-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 24113; Mature: 24113

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPSLKYCGIHSLEDLKVTARSKADYLGFIFAESKRSVDPADVKRWCGETDTPGKKLVG
CCCCCCCCCCCCCCHHHEEHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHEEE
VFVNENMSRMAEIVKDAGLDVIQLHGDETAADIKRLKSMTDCEIWKALPHGNDTVQSMAS
EEECCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH
FAPYIDGYVIDSSVKGMRGGTGVSFSWDSVPLYIDAAQREGKRLFIAGGVNPDTIADLLK
HHHHHCCEEECCCCCCCCCCCCCEEECCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHH
RRPPGIDLASGIEERGRKSEKLISLLEERMFEHVFISE
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKPSLKYCGIHSLEDLKVTARSKADYLGFIFAESKRSVDPADVKRWCGETDTPGKKLVG
CCCCCCCCCCCCCCHHHEEHHCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCHHHEEE
VFVNENMSRMAEIVKDAGLDVIQLHGDETAADIKRLKSMTDCEIWKALPHGNDTVQSMAS
EEECCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHH
FAPYIDGYVIDSSVKGMRGGTGVSFSWDSVPLYIDAAQREGKRLFIAGGVNPDTIADLLK
HHHHHCCEEECCCCCCCCCCCCCEEECCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHH
RRPPGIDLASGIEERGRKSEKLISLLEERMFEHVFISE
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA