Click here to switch to the map view.

The map label for this gene is proJ

Identifier: 52785900

GI number: 52785900

Start: 2097983

End: 2099104

Strand: Reverse

Name: proJ

Synonym: BLi02149

Alternate gene names: 52785900

Gene position: 2099104-2097983 (Counterclockwise)

Preceding gene: 52785901

Following gene: 52785899

Centisome position: 49.71

GC content: 48.04

Gene sequence:

>1122_bases
ATGACCTCTAATCAACAGAGGGTAGTTGTCAAAGTGGGAAGCAGTTCTTTAACAAGTCTTCACGGCGAAATCAGCAGCAA
AAAGCTCGAAAAGCTCGTTGAGCAGATTGTCCGCCTCAAAGATGAAGGACACGAAGTCCTCCTTGTTTCATCTGGCGCTG
TAGCAGCCGGTTACCGGAAGCTCGGCTTTATCAACAGGCCGGATACATTGCCTGAAAAACAAGCATCCGCTTCTGTCGGC
CAAGGCTTATTAATCGAAGCGTATTCCAAGCTTTTTTTATCCCACGGCTATATCGCTTCACAAATTTTAATCACAAAAAG
CGATTTTTCAGATGAAAAACGGTACAACAATGTGAGAAATACGATGAACGTGCTGCTGAAACGCGGCATTATTCCGATCA
TCAACGAAAATGATACGGTTACCGTCAATCGGCTCAAATTCGGAGACAATGACACGCTTTCTGCTAAAGTGGCGGGTCTG
ATCGACGCCGACCTGCTGACCATCCTCTCTGATATAGACGGCCTTTATGACGCGAATCCGCGAAAAACGCCGGACGCCGC
ACTCGTCCAGGAAGTAACTGAAATCACGCCGGAAATAGAAGCTTCAGCAGGGGATGCAGGAAGCGAAGTCGGAACCGGCG
GCATGAAGTCCAAACTGGACGCTTTTAAAATTACAATGGCTTCAGGCATCAAAGGGTTTTTAGGGCGCGCGGACACCCCC
GGCATTTTGCACGAAGCGATCTGCGGGACTGCGAGGGGAACGTATTTTATACCGAATGCGGATTCACACGCCCTCAACCA
TAAAAAGCAATGGATAGCATTTAACTCTGGACCTGAAGGAGACATCGTCGTCCACAACGAAAGCAAAGAGTTTATTATCG
ACCGTAAAAAAAGCCTGTATCCTTCTAGTATTTACGACATCAACGGCCGTTTCAGCAACGGATCCGTCGTTCGAATCCTT
GATCAGGAAGGAGAGGAAATCGGCCTCGGCGTCGTCAACTTTTCCTCATCCCAGCTTCGTAAATGGAAGAAGGATACGAA
TCAGGAAACAGCCGCTTCCTCACAGGCTGTCGTTGACAGAGAAGCGTTTGTCTGTCATTTAGAGCTATCCGTTCCGCTTT
AA

Upstream 100 bases:

>100_bases
AAGCCGCAAAACGATCCAAAACGATCAGTCTCGAACTGGAACAATTAGTCGCAGCAAAGTAAATGCCCGTAACGAAGAAA
AATACTACAGGAGTGATAAA

Downstream 100 bases:

>100_bases
AAAACAAATCAAAGGAGGAATTGACATGACTGCGACGAGCGAGAGCATCAGTGCACAAATCGAAGAAAAAGCGATAAAGG
CAAAAACGGCCGCAAAATCT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 2; GK 2

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTSNQQRVVVKVGSSSLTSLHGEISSKKLEKLVEQIVRLKDEGHEVLLVSSGAVAAGYRKLGFINRPDTLPEKQASASVG
QGLLIEAYSKLFLSHGYIASQILITKSDFSDEKRYNNVRNTMNVLLKRGIIPIINENDTVTVNRLKFGDNDTLSAKVAGL
IDADLLTILSDIDGLYDANPRKTPDAALVQEVTEITPEIEASAGDAGSEVGTGGMKSKLDAFKITMASGIKGFLGRADTP
GILHEAICGTARGTYFIPNADSHALNHKKQWIAFNSGPEGDIVVHNESKEFIIDRKKSLYPSSIYDINGRFSNGSVVRIL
DQEGEEIGLGVVNFSSSQLRKWKKDTNQETAASSQAVVDREAFVCHLELSVPL

Sequences:

>Translated_373_residues
MTSNQQRVVVKVGSSSLTSLHGEISSKKLEKLVEQIVRLKDEGHEVLLVSSGAVAAGYRKLGFINRPDTLPEKQASASVG
QGLLIEAYSKLFLSHGYIASQILITKSDFSDEKRYNNVRNTMNVLLKRGIIPIINENDTVTVNRLKFGDNDTLSAKVAGL
IDADLLTILSDIDGLYDANPRKTPDAALVQEVTEITPEIEASAGDAGSEVGTGGMKSKLDAFKITMASGIKGFLGRADTP
GILHEAICGTARGTYFIPNADSHALNHKKQWIAFNSGPEGDIVVHNESKEFIIDRKKSLYPSSIYDINGRFSNGSVVRIL
DQEGEEIGLGVVNFSSSQLRKWKKDTNQETAASSQAVVDREAFVCHLELSVPL
>Mature_372_residues
TSNQQRVVVKVGSSSLTSLHGEISSKKLEKLVEQIVRLKDEGHEVLLVSSGAVAAGYRKLGFINRPDTLPEKQASASVGQ
GLLIEAYSKLFLSHGYIASQILITKSDFSDEKRYNNVRNTMNVLLKRGIIPIINENDTVTVNRLKFGDNDTLSAKVAGLI
DADLLTILSDIDGLYDANPRKTPDAALVQEVTEITPEIEASAGDAGSEVGTGGMKSKLDAFKITMASGIKGFLGRADTPG
ILHEAICGTARGTYFIPNADSHALNHKKQWIAFNSGPEGDIVVHNESKEFIIDRKKSLYPSSIYDINGRFSNGSVVRILD
QEGEEIGLGVVNFSSSQLRKWKKDTNQETAASSQAVVDREAFVCHLELSVPL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=292, Percent_Identity=31.1643835616438, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI62912457, Length=290, Percent_Identity=30.6896551724138, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1786437, Length=350, Percent_Identity=39.1428571428571, Blast_Score=251, Evalue=7e-68,
Organism=Caenorhabditis elegans, GI17569969, Length=343, Percent_Identity=29.4460641399417, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=343, Percent_Identity=29.4460641399417, Blast_Score=131, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=348, Percent_Identity=36.4942528735632, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6320506, Length=392, Percent_Identity=33.4183673469388, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=32.0689655172414, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB2_BACLD (Q65IS8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079312.1
- RefSeq:   YP_091729.1
- ProteinModelPortal:   Q65IS8
- SMR:   Q65IS8
- STRING:   Q65IS8
- EnsemblBacteria:   EBBACT00000056993
- EnsemblBacteria:   EBBACT00000061067
- GeneID:   3031438
- GeneID:   3100671
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02149
- KEGG:   bli:BL01983
- NMPDR:   fig|279010.5.peg.2845
- eggNOG:   COG0263
- GeneTree:   EBGT00050000001887
- HOGENOM:   HBG507643
- OMA:   NVRNTMN
- ProtClustDB:   PRK13402
- BioCyc:   BLIC279010-1:BLI02149-MONOMER
- BioCyc:   BLIC279010:BL01983-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40474; Mature: 40343

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNQQRVVVKVGSSSLTSLHGEISSKKLEKLVEQIVRLKDEGHEVLLVSSGAVAAGYRK
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
LGFINRPDTLPEKQASASVGQGLLIEAYSKLFLSHGYIASQILITKSDFSDEKRYNNVRN
HCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHH
TMNVLLKRGIIPIINENDTVTVNRLKFGDNDTLSAKVAGLIDADLLTILSDIDGLYDANP
HHHHHHHCCCEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RKTPDAALVQEVTEITPEIEASAGDAGSEVGTGGMKSKLDAFKITMASGIKGFLGRADTP
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEHHHCHHHHHCCCCCC
GILHEAICGTARGTYFIPNADSHALNHKKQWIAFNSGPEGDIVVHNESKEFIIDRKKSLY
HHHHHHHHCCCCCCEECCCCCCHHHCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCC
PSSIYDINGRFSNGSVVRILDQEGEEIGLGVVNFSSSQLRKWKKDTNQETAASSQAVVDR
CCCEEECCCEECCCCEEEEEECCCCEEEEEEEECCHHHHHHHHHCCCHHHHCCCHHHCCC
EAFVCHLELSVPL
CEEEEEEEEECCC
>Mature Secondary Structure 
TSNQQRVVVKVGSSSLTSLHGEISSKKLEKLVEQIVRLKDEGHEVLLVSSGAVAAGYRK
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
LGFINRPDTLPEKQASASVGQGLLIEAYSKLFLSHGYIASQILITKSDFSDEKRYNNVRN
HCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHH
TMNVLLKRGIIPIINENDTVTVNRLKFGDNDTLSAKVAGLIDADLLTILSDIDGLYDANP
HHHHHHHCCCEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RKTPDAALVQEVTEITPEIEASAGDAGSEVGTGGMKSKLDAFKITMASGIKGFLGRADTP
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEEHHHCHHHHHCCCCCC
GILHEAICGTARGTYFIPNADSHALNHKKQWIAFNSGPEGDIVVHNESKEFIIDRKKSLY
HHHHHHHHCCCCCCEECCCCCCHHHCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCC
PSSIYDINGRFSNGSVVRILDQEGEEIGLGVVNFSSSQLRKWKKDTNQETAASSQAVVDR
CCCEEECCCEECCCCEEEEEECCCCEEEEEEEECCHHHHHHHHHCCCHHHHCCCHHHCCC
EAFVCHLELSVPL
CEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA