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The map label for this gene is proA2

Identifier: 52785899

GI number: 52785899

Start: 2096686

End: 2097957

Strand: Reverse

Name: proA2

Synonym: BLi02148

Alternate gene names: 52785899

Gene position: 2097957-2096686 (Counterclockwise)

Preceding gene: 52785900

Following gene: 52785898

Centisome position: 49.68

GC content: 50.31

Gene sequence:

>1272_bases
ATGACTGCGACGAGCGAGAGCATCAGTGCACAAATCGAAGAAAAAGCGATAAAGGCAAAAACGGCCGCAAAATCTTTGCG
GCTGGCAACTGAAAAAGAAAAGAACGAGGCGCTTGCTGCATTGGCCGACCACCTGAAACAAAACATGAGCTATATTCTCA
CTGAAAATGCCAAAGATATCGAAGCGGGACGAAAAAAAGGCTATGATGCAGCCTACATCGACCGCCTGTCGCTCAATGAA
GACAGGGTTCTGGATTTTGCCGATGGGCTGCTGGAAGTCGCGGAACTTGATGATCCGGTCGGAGAAACGCTATCAAGCTG
GACGCTCGACAACGGGTTAAAAGCGGAAAAAGTCAGCGTTCCTCTCGGCGTCATCGGCATGATCTATGAAGCGCGTCCGA
ACGTAACTGTCGATGCGACCGGGCTTGCTCTAAAATCAGGCAATGCGATCGTTTTGAAAGGCGGCTCTTCCGCCATTCAT
TCGAATACAGCGATCGTATCGGTCATGCATCAAGCGCTTGATTCGACAAACATCCCCCGCGATGCCGTTCAATTCATCGA
AAGCACAGACCGAAGCGCAACGAAGCAGCTCTTTAAGATGAAAGAACATATCGATGTACTCATCCCCCGGGGAGGCGGGG
CCCTCATTCAGGAAGTCGTTGAGAACGCAACCGTTCCGGTCCTTGAAACGGGAGTCGGCAACTGCCACATCTATATTGAC
CGGGAAGCGGACCCTGAAAAAGCGATCGATGTCATGATCAATGCGAAAACAGACCGCCCCGCCGTCTGCAATGCACTTGA
AACGCTGATCGTCCACGAAAAATGGCTTGAGGAAAACAGCGCCGCGCTTATCAATGCTTTAAACGAACACGGGATTCAAG
TTCACGGGGACGAAAAGGCTGTTCAAATGATTCCAGGCGCAAGACCAGCAGGGGAAGCCGATTGGCAGAACGAATATTTA
AGCCTTGATCTCGCGGTTAAAGTCGCAGGAAGTCTTGAAGAAGCCTGCGATCATATCGAAACATACGGAACAAAGCATTC
AGAAGCGATTATCACCGAAAATCCGGAAACAGCCAAAGCTTTTCTGAATACCGTTGATGCTGCAGCATTGTACCACAATG
CCTCAACCAGGTTCACCGACGGCGGCGCACTCGGATTCGGCGCTGAAATCGGAATTTCTACACAGAAGCTTCACGCCCGC
GGACCTATGGGTCTGAAAGAATTGACCACATGCAAATATATCATGAGAGGCGACGGCCATATCAGAAGATAA

Upstream 100 bases:

>100_bases
ACAGCCGCTTCCTCACAGGCTGTCGTTGACAGAGAAGCGTTTGTCTGTCATTTAGAGCTATCCGTTCCGCTTTAAAAAAC
AAATCAAAGGAGGAATTGAC

Downstream 100 bases:

>100_bases
ATAAAGAGACCTGTACTGAAACTGCACCTGGTTTGTCAGACACCATCTGACAATCCGGAGGTGCAGTTTTTTTCGGCTGA
ATTTACATCCTCATAAAACA

Product: hypothetical protein

Products: NA

Alternate protein names: GPR 2; Glutamate-5-semialdehyde dehydrogenase 2; Glutamyl-gamma-semialdehyde dehydrogenase 2; GSA dehydrogenase 2

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTATSESISAQIEEKAIKAKTAAKSLRLATEKEKNEALAALADHLKQNMSYILTENAKDIEAGRKKGYDAAYIDRLSLNE
DRVLDFADGLLEVAELDDPVGETLSSWTLDNGLKAEKVSVPLGVIGMIYEARPNVTVDATGLALKSGNAIVLKGGSSAIH
SNTAIVSVMHQALDSTNIPRDAVQFIESTDRSATKQLFKMKEHIDVLIPRGGGALIQEVVENATVPVLETGVGNCHIYID
READPEKAIDVMINAKTDRPAVCNALETLIVHEKWLEENSAALINALNEHGIQVHGDEKAVQMIPGARPAGEADWQNEYL
SLDLAVKVAGSLEEACDHIETYGTKHSEAIITENPETAKAFLNTVDAAALYHNASTRFTDGGALGFGAEIGISTQKLHAR
GPMGLKELTTCKYIMRGDGHIRR

Sequences:

>Translated_423_residues
MTATSESISAQIEEKAIKAKTAAKSLRLATEKEKNEALAALADHLKQNMSYILTENAKDIEAGRKKGYDAAYIDRLSLNE
DRVLDFADGLLEVAELDDPVGETLSSWTLDNGLKAEKVSVPLGVIGMIYEARPNVTVDATGLALKSGNAIVLKGGSSAIH
SNTAIVSVMHQALDSTNIPRDAVQFIESTDRSATKQLFKMKEHIDVLIPRGGGALIQEVVENATVPVLETGVGNCHIYID
READPEKAIDVMINAKTDRPAVCNALETLIVHEKWLEENSAALINALNEHGIQVHGDEKAVQMIPGARPAGEADWQNEYL
SLDLAVKVAGSLEEACDHIETYGTKHSEAIITENPETAKAFLNTVDAAALYHNASTRFTDGGALGFGAEIGISTQKLHAR
GPMGLKELTTCKYIMRGDGHIRR
>Mature_422_residues
TATSESISAQIEEKAIKAKTAAKSLRLATEKEKNEALAALADHLKQNMSYILTENAKDIEAGRKKGYDAAYIDRLSLNED
RVLDFADGLLEVAELDDPVGETLSSWTLDNGLKAEKVSVPLGVIGMIYEARPNVTVDATGLALKSGNAIVLKGGSSAIHS
NTAIVSVMHQALDSTNIPRDAVQFIESTDRSATKQLFKMKEHIDVLIPRGGGALIQEVVENATVPVLETGVGNCHIYIDR
EADPEKAIDVMINAKTDRPAVCNALETLIVHEKWLEENSAALINALNEHGIQVHGDEKAVQMIPGARPAGEADWQNEYLS
LDLAVKVAGSLEEACDHIETYGTKHSEAIITENPETAKAFLNTVDAAALYHNASTRFTDGGALGFGAEIGISTQKLHARG
PMGLKELTTCKYIMRGDGHIRR

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=34.8668280871671, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=34.8668280871671, Blast_Score=259, Evalue=4e-69,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=45.0602409638554, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17569969, Length=414, Percent_Identity=37.1980676328502, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17569967, Length=414, Percent_Identity=37.1980676328502, Blast_Score=275, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6324899, Length=440, Percent_Identity=36.8181818181818, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI21357643, Length=424, Percent_Identity=37.5, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA2_BACLD (Q65IS9)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079311.1
- RefSeq:   YP_091728.1
- ProteinModelPortal:   Q65IS9
- SMR:   Q65IS9
- STRING:   Q65IS9
- EnsemblBacteria:   EBBACT00000057838
- EnsemblBacteria:   EBBACT00000059563
- GeneID:   3031437
- GeneID:   3097479
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02148
- KEGG:   bli:BL01984
- NMPDR:   fig|279010.5.peg.2844
- eggNOG:   COG0014
- GeneTree:   EBGT00050000000020
- HOGENOM:   HBG318080
- OMA:   IPRDAVQ
- ProtClustDB:   CLSK929025
- BioCyc:   BLIC279010-1:BLI02148-MONOMER
- BioCyc:   BLIC279010:BL01984-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45601; Mature: 45470

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATSESISAQIEEKAIKAKTAAKSLRLATEKEKNEALAALADHLKQNMSYILTENAKDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
EAGRKKGYDAAYIDRLSLNEDRVLDFADGLLEVAELDDPVGETLSSWTLDNGLKAEKVSV
HHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEC
PLGVIGMIYEARPNVTVDATGLALKSGNAIVLKGGSSAIHSNTAIVSVMHQALDSTNIPR
CHHHHHHHEECCCCEEEEECCEEEECCCEEEEECCCCCHHCCHHHHHHHHHHHCCCCCCH
DAVQFIESTDRSATKQLFKMKEHIDVLIPRGGGALIQEVVENATVPVLETGVGNCHIYID
HHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCEEECCCCEEEEEEE
READPEKAIDVMINAKTDRPAVCNALETLIVHEKWLEENSAALINALNEHGIQVHGDEKA
CCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHH
VQMIPGARPAGEADWQNEYLSLDLAVKVAGSLEEACDHIETYGTKHSEAIITENPETAKA
HHCCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCHHHHH
FLNTVDAAALYHNASTRFTDGGALGFGAEIGISTQKLHARGPMGLKELTTCKYIMRGDGH
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCC
IRR
CCC
>Mature Secondary Structure 
TATSESISAQIEEKAIKAKTAAKSLRLATEKEKNEALAALADHLKQNMSYILTENAKDI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHH
EAGRKKGYDAAYIDRLSLNEDRVLDFADGLLEVAELDDPVGETLSSWTLDNGLKAEKVSV
HHHHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEC
PLGVIGMIYEARPNVTVDATGLALKSGNAIVLKGGSSAIHSNTAIVSVMHQALDSTNIPR
CHHHHHHHEECCCCEEEEECCEEEECCCEEEEECCCCCHHCCHHHHHHHHHHHCCCCCCH
DAVQFIESTDRSATKQLFKMKEHIDVLIPRGGGALIQEVVENATVPVLETGVGNCHIYID
HHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCEEECCCCEEEEEEE
READPEKAIDVMINAKTDRPAVCNALETLIVHEKWLEENSAALINALNEHGIQVHGDEKA
CCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHH
VQMIPGARPAGEADWQNEYLSLDLAVKVAGSLEEACDHIETYGTKHSEAIITENPETAKA
HHCCCCCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEECCCHHHHH
FLNTVDAAALYHNASTRFTDGGALGFGAEIGISTQKLHARGPMGLKELTTCKYIMRGDGH
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCC
IRR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA